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公开(公告)号:US20060292591A1
公开(公告)日:2006-12-28
申请号:US11349066
申请日:2006-02-06
申请人: Joe Gray , Colin Collins , Soo-in Hwang , Tony Godfrey , David Kowbel , Johanna Rommens
发明人: Joe Gray , Colin Collins , Soo-in Hwang , Tony Godfrey , David Kowbel , Johanna Rommens
CPC分类号: C12Q1/6886 , C07K14/47 , C07K14/4702 , C07K14/4705 , C07K14/82 , C12N9/1205 , C12N9/90 , C12Q2600/112 , C12Q2600/118 , G01N33/57484
摘要: The present invention relates to cDNA sequences from a region of amplification on chromosome 20 associated with disease. The sequences can be used in hybridization methods for the identification of chromosomal abnormalities associated with various diseases. The sequences can also be used for treatment of diseases.
摘要翻译: 本发明涉及与染色体20相关的扩增区域的cDNA序列。 该序列可以用于鉴定与各种疾病相关的染色体异常的杂交方法中。 序列也可用于治疗疾病。
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公开(公告)号:US5892010A
公开(公告)日:1999-04-06
申请号:US680395
申请日:1996-07-15
申请人: Joe Gray , Colin Collins , Soo-in Hwang , Tony Godfrey , David Kowbel , Johanna Rommens
发明人: Joe Gray , Colin Collins , Soo-in Hwang , Tony Godfrey , David Kowbel , Johanna Rommens
CPC分类号: C12N9/1205 , C07K14/47 , C07K14/4705 , C07K14/82 , C12N9/90 , C12Q1/6886 , C12Q2600/112 , C12Q2600/118
摘要: The present invention relates to cDNA sequences from a region of amplification on chromosome 20 associated with disease. The sequences can be used in hybridization methods for the identification of chromosomal abnormalities associated with various diseases. The sequences can also be used for treatment of diseases.
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公开(公告)号:US20070037202A1
公开(公告)日:2007-02-15
申请号:US11583305
申请日:2006-10-18
申请人: Colin Collins , Guiqing Huang , Joe Gray
发明人: Colin Collins , Guiqing Huang , Joe Gray
IPC分类号: C12Q1/68 , A61K31/704
CPC分类号: C07K14/82 , A61K38/00 , C07K14/4748 , G01N33/5011 , G01N33/5014 , G01N33/57484 , G01N2510/00 , G01N2800/52
摘要: This invention provides methods, reagents and kits for treating cancer in a patient or subject, e.g., a human. Accordingly, the present methods can be used to monitor the efficacy of a cancer treatment and to treat cancer, e.g., by inhibiting the expression and/or activity of ZNF217 in a neoplastic cell.
摘要翻译: 本发明提供了用于治疗患者或受试者例如人的癌症的方法,试剂和试剂盒。 因此,本发明的方法可用于监测癌症治疗的功效和治疗癌症,例如通过抑制肿瘤细胞中ZNF217的表达和/或活性。
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4.
公开(公告)号:US06465182B1
公开(公告)日:2002-10-15
申请号:US09302056
申请日:1999-04-29
申请人: Joe Gray , Dan Pinkel , Donna Albertson , Colin Collins , Russell Baldocchi
发明人: Joe Gray , Dan Pinkel , Donna Albertson , Colin Collins , Russell Baldocchi
IPC分类号: C12Q168
CPC分类号: C12Q1/6809 , C12Q1/6855 , C12Q2525/155 , C12Q2525/161 , C12Q2565/519 , C12Q2539/107 , C12Q2537/143
摘要: The present invention provides methods of determining relative copy number of target nucleic acid sequences and precise mapping of chromosomal abnormalities associated with disease. The methods of the invention use target nucleic acid sequences immobilized on a solid surface, to which a sample comprising two sets of differentially labeled nucleic acid sequences are hybridized. The hybridization of the labeled nucleic acid sequences to the solid surface is then detected using standard techniques.
摘要翻译: 本发明提供确定靶核酸序列的相对拷贝数和与疾病相关的染色体异常的精确定位的方法。 本发明的方法使用固定在固体表面上的靶核酸序列,其中包含两组差异标记的核酸序列的样品与之杂交。 然后使用标准技术检测标记的核酸序列与固体表面的杂交。
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5.
公开(公告)号:US20060257895A1
公开(公告)日:2006-11-16
申请号:US11361316
申请日:2006-02-24
申请人: Daniel Pinkel , Joe Gray , Anne Kallioniemi , Ollie-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
发明人: Daniel Pinkel , Joe Gray , Anne Kallioniemi , Ollie-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6809 , C12Q1/6841 , C12Q1/6886 , C12Q2600/156 , G06F15/025 , Y10S436/813 , C12Q2545/114 , C12Q2537/157
摘要: Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).
摘要翻译: 公开了包括使用原位杂交来检测一个或多个基因组中的异常核酸序列拷贝数的新方法,其中结合参考染色体扩增的多个基因座的重复序列基本上被去除和/或其杂交信号被抑制。 称为比较基因组杂交(CGH)的发明提供了确定一个或多个受试者基因组或其部分(例如肿瘤细胞)中核酸序列的相对拷贝数的方法,作为这些序列的位置的函数 参考基因组(例如,正常人类基因组)。 比较来自每个标记的对象核酸的信号的强度和/或来自标记的目标核酸序列的不同信号之间的比率差异,以确定一个或多个核酸序列中核酸序列的相对拷贝数 主题基因组作为沿着参考染色体扩散的位置的函数。 可以检测主题基因组中的扩增,重复和/或缺失。 还提供了确定受试细胞或细胞群体中基本上所有RNA或DNA序列的绝对拷贝数的方法。
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公开(公告)号:US20060063168A1
公开(公告)日:2006-03-23
申请号:US11060644
申请日:2005-02-16
申请人: Donna Albertson , Daniel Pinkel , Jane Fridyland , Bing Huey , Antoine Snijders , Joe Gray , Anne Kallioniemi , Olli-Pekka Kallioniemi , Frederic Waldman
发明人: Donna Albertson , Daniel Pinkel , Jane Fridyland , Bing Huey , Antoine Snijders , Joe Gray , Anne Kallioniemi , Olli-Pekka Kallioniemi , Frederic Waldman
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6841 , C12Q2600/156 , C12Q2565/601
摘要: The present invention provides a method of detecting nucleotide sequence differences between two nucleic acid samples. The method employs a comparative genomic hybridization (CGH) technique to analyze the sequence differences between the samples. This method permits the identification of small sequence differences (e.g., sequence divergence of 1% or less) in nucleic acid samples of high complexity (e.g., an entire genome).
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公开(公告)号:US20060292608A1
公开(公告)日:2006-12-28
申请号:US11431094
申请日:2006-05-08
CPC分类号: C12Q1/6841 , C12Q1/6809 , C12Q1/6853 , C12Q1/6886 , C12Q2600/158 , C12Q2545/114 , C12Q2545/113 , C12Q2537/143 , C12Q2525/151 , C12Q2563/107
摘要: Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).
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公开(公告)号:US20050137389A1
公开(公告)日:2005-06-23
申请号:US10932799
申请日:2004-09-01
申请人: Joe Gray , Daniel Pinkel
发明人: Joe Gray , Daniel Pinkel
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6886 , C12Q1/6811 , C12Q1/6841 , C12Q1/6853 , C12Q1/6879 , C12Q2600/156 , C12Q2563/131 , C12Q2563/107 , C12Q2525/151
摘要: The invention relates generally to the field of cytogenetics, and more particularly to methods for identifying and classifying chromosomes.
摘要翻译: 本发明一般涉及细胞遗传学领域,更具体地涉及鉴定和分类染色体的方法。
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公开(公告)号:US20050048561A1
公开(公告)日:2005-03-03
申请号:US10960224
申请日:2004-10-06
申请人: Mack Fulwyler , Joe Gray
发明人: Mack Fulwyler , Joe Gray
IPC分类号: G01N33/558 , C12Q1/68 , C12M1/34 , G01N33/53
CPC分类号: B01L3/502784 , B01J19/0046 , B01J2219/00479 , B01J2219/00511 , B01J2219/0052 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00628 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00657 , B01J2219/00722 , B01L2200/0673 , B01L2300/0636 , B01L2300/0816 , B01L2300/0838 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0487 , G01N33/558 , Y10S436/807 , Y10T436/117497
摘要: The invention provides methods and devices for detecting the presence of one or more target analytes in a sample employing a channel having affixed therein one or more binding partners for each target analyte. Assays are carried out by transporting the sample through the channel to each successive binding partner so that target analyte present in said sample binds to the corresponding binding partner. The sample is then transported beyond the binding partner(s), followed by detection of any target analyte bound to each binding partner. In one embodiment, binding efficiency is increased by the use of segmented transport, wherein a first bolus or bubble of a fluid that is immiscible with the sample precedes the sample during transport and a second bolus or bubble of a fluid that is immiscible with the sample follows the sample. Many configurations are possible for the device of the invention. A preferred device includes: a substrate with a channel formed in its surface, and a cover element that overlies and seals the channel. Binding partner(s) are affixed to the surface of the cover element facing the channel lumen.
摘要翻译: 本发明提供用于检测样品中存在一种或多种目标分析物的方法和装置,其中使用其中固定有每个目标分析物的一个或多个结合配偶体的通道。 通过将样品通过通道输送到每个连续的结合配偶体进行测定,使得存在于所述样品中的目标分析物与相应的结合配偶体结合。 然后将样品转移超过结合配偶体,随后检测与每个结合配偶体结合的任何目标分析物。 在一个实施方案中,通过使用分段运输来增加结合效率,其中在运输期间与样品不混溶的流体的第一推注或气泡先于样品,并且与样品不混溶的流体的第二推注或气泡 遵循样本。 许多配置对于本发明的设备是可能的。 优选的装置包括:具有在其表面中形成的通道的基底和覆盖并密封通道的盖元件。 绑定伙伴被固定在覆盖元件面向通道腔的表面上。
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公开(公告)号:US07785813B2
公开(公告)日:2010-08-31
申请号:US11471464
申请日:2006-06-19
申请人: Joe Gray , Graeme Hodgson , Douglas Hanahan , Jeffrey Hager , Oriol Casanovas
发明人: Joe Gray , Graeme Hodgson , Douglas Hanahan , Jeffrey Hager , Oriol Casanovas
CPC分类号: G01N33/57407 , C12Q1/6886 , C12Q2600/136 , C12Q2600/158 , G01N33/57496 , G01N2800/52
摘要: This invention provides methods employing prefoldin-4 (PFDN-4) nucleic acid and polypeptide sequences to detect cancer or a propensity to develop cancer, to monitor the efficacy of a cancer treatment, and/or for prognostic applications. Further, the invention provides methods of identifying inhibitors of PfDN-4 and methods of treating cancer by inhibiting the expression and/or activity of PFDN-4.
摘要翻译: 本发明提供了使用预折合蛋白-4(PFDN-4)核酸和多肽序列来检测癌症或倾向于发展癌症,监测癌症治疗的功效和/或预后应用的方法。 此外,本发明提供了鉴定PfDN-4抑制剂的方法和通过抑制PFDN-4的表达和/或活性来治疗癌症的方法。
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