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    Detection of chromosomal abnormalities associated with breast cancer
    3.
    发明申请
    Detection of chromosomal abnormalities associated with breast cancer 失效
    检测与乳腺癌相关的染色体异常

    公开(公告)号：US20060257895A1

    公开(公告)日：2006-11-16

    申请号：US11361316

    申请日：2006-02-24

    申请人： Daniel Pinkel ,  Joe Gray ,  Anne Kallioniemi ,  Ollie-Pekka Kallioniemi ,  Frederic Waldman ,  Masaru Sakamoto

    发明人： Daniel Pinkel ,  Joe Gray ,  Anne Kallioniemi ,  Ollie-Pekka Kallioniemi ,  Frederic Waldman ,  Masaru Sakamoto

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6809 ,  C12Q1/6841 ,  C12Q1/6886 ,  C12Q2600/156 ,  G06F15/025 ,  Y10S436/813 ,  C12Q2545/114 ,  C12Q2537/157

    摘要： Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).

    摘要翻译： 公开了包括使用原位杂交来检测一个或多个基因组中的异常核酸序列拷贝数的新方法，其中结合参考染色体扩增的多个基因座的重复序列基本上被去除和/或其杂交信号被抑制。 称为比较基因组杂交（CGH）的发明提供了确定一个或多个受试者基因组或其部分（例如肿瘤细胞）中核酸序列的相对拷贝数的方法，作为这些序列的位置的函数 参考基因组（例如，正常人类基因组）。 比较来自每个标记的对象核酸的信号的强度和/或来自标记的目标核酸序列的不同信号之间的比率差异，以确定一个或多个核酸序列中核酸序列的相对拷贝数 主题基因组作为沿着参考染色体扩散的位置的函数。 可以检测主题基因组中的扩增，重复和/或缺失。 还提供了确定受试细胞或细胞群体中基本上所有RNA或DNA序列的绝对拷贝数的方法。

    Comparative genomic hybridization
    5.
    发明申请
    Comparative genomic hybridization 失效

    公开(公告)号：US20060292608A1

    公开(公告)日：2006-12-28

    申请号：US11431094

    申请日：2006-05-08

    申请人： Daniel Pinkel ,  Joe Gray ,  Anne Kallioniemi ,  Olli-Pekka Kallioniemi ,  Frederic Waldman

    发明人： Daniel Pinkel ,  Joe Gray ,  Anne Kallioniemi ,  Olli-Pekka Kallioniemi ,  Frederic Waldman

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6841 ,  C12Q1/6809 ,  C12Q1/6853 ,  C12Q1/6886 ,  C12Q2600/158 ,  C12Q2545/114 ,  C12Q2545/113 ,  C12Q2537/143 ,  C12Q2525/151 ,  C12Q2563/107

    摘要： Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).

    Capillary array and related methods
    7.
    发明申请
    Capillary array and related methods 有权
    毛细管阵列及相关方法

    公开(公告)号：US20050048561A1

    公开(公告)日：2005-03-03

    申请号：US10960224

    申请日：2004-10-06

    申请人： Mack Fulwyler ,  Joe Gray

    发明人： Mack Fulwyler ,  Joe Gray

    IPC分类号： G01N33/558 ,  C12Q1/68 ,  C12M1/34 ,  G01N33/53

    CPC分类号： B01L3/502784 ,  B01J19/0046 ,  B01J2219/00479 ,  B01J2219/00511 ,  B01J2219/0052 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00657 ,  B01J2219/00722 ,  B01L2200/0673 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0487 ,  G01N33/558 ,  Y10S436/807 ,  Y10T436/117497

    摘要： The invention provides methods and devices for detecting the presence of one or more target analytes in a sample employing a channel having affixed therein one or more binding partners for each target analyte. Assays are carried out by transporting the sample through the channel to each successive binding partner so that target analyte present in said sample binds to the corresponding binding partner. The sample is then transported beyond the binding partner(s), followed by detection of any target analyte bound to each binding partner. In one embodiment, binding efficiency is increased by the use of segmented transport, wherein a first bolus or bubble of a fluid that is immiscible with the sample precedes the sample during transport and a second bolus or bubble of a fluid that is immiscible with the sample follows the sample. Many configurations are possible for the device of the invention. A preferred device includes: a substrate with a channel formed in its surface, and a cover element that overlies and seals the channel. Binding partner(s) are affixed to the surface of the cover element facing the channel lumen.

    摘要翻译： 本发明提供用于检测样品中存在一种或多种目标分析物的方法和装置，其中使用其中固定有每个目标分析物的一个或多个结合配偶体的通道。 通过将样品通过通道输送到每个连续的结合配偶体进行测定，使得存在于所述样品中的目标分析物与相应的结合配偶体结合。 然后将样品转移超过结合配偶体，随后检测与每个结合配偶体结合的任何目标分析物。 在一个实施方案中，通过使用分段运输来增加结合效率，其中在运输期间与样品不混溶的流体的第一推注或气泡先于样品，并且与样品不混溶的流体的第二推注或气泡 遵循样本。 许多配置对于本发明的设备是可能的。 优选的装置包括：具有在其表面中形成的通道的基底和覆盖并密封通道的盖元件。 绑定伙伴被固定在覆盖元件面向通道腔的表面上。