公开(公告)号：US20060064763A1

公开(公告)日：2006-03-23

申请号：US11265114

申请日：2005-11-03

申请人： Keith Campbell ,  Ian Wilmut

发明人： Keith Campbell ,  Ian Wilmut

IPC分类号： A01K67/027

CPC分类号： C12N15/8773 ,  A61K35/54 ,  C12N15/873 ,  C12N15/8771

摘要： A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at the time of transfer, so that the donor nucleus is kept exposed to the recipient cytoplasm for a period of time. The diploid nucleus can be donated by a cell in either the G0 or G1 phase of the cell cycle at the time of transfer. Subsequently, the reconstituted embryo is activated. Correct ploidy is maintained during activation, for example, by incubating the reconstituted embryo in the presence of a microtubule inhibitor such as nocodazole. The reconstituted embryo may then give rise to one or more live animal births. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.

公开(公告)号：US20050172347A1

公开(公告)日：2005-08-04

申请号：US11068903

申请日：2005-03-02

申请人： Keith Campbell ,  Ian Wilmut

发明人： Keith Campbell ,  Ian Wilmut

IPC分类号： A01K67/02 ,  A61K35/54 ,  C12N5/10 ,  C12N15/02 ,  C12N15/873 ,  C12N15/877 ,  A01K67/027

CPC分类号： C12N15/8773 ,  A61K35/54 ,  C12N15/873 ,  C12N15/8771

摘要： A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at the time of transfer, so that the donor nucleus is kept exposed to the recipient cytoplasm for a period of time. The diploid nucleus can be donated by a cell in either the G0 or G1 phase of the cell cycle at the time of transfer. Subsequently, the reconstituted embryo is activated. Correct ploidy is maintained during activation, for example, by incubating the reconstituted embryo in the presence of a microtubule inhibitor such as nocodazole. The reconstituted embryo may then give rise to one or more live animal births. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.

摘要翻译： 重建动物胚胎的方法涉及将二倍体核转移到在第二次减数分裂期的中期被逮捕的卵母细胞。 卵母细胞在转移时不被激活，使得供者核细胞保持暴露于受体细胞质一段时间。 二倍体核可以在转移时由细胞G0或G1期的细胞捐献。 随后，重建的胚胎被激活。 在活化期间维持正确的倍性，例如通过在微管抑制剂例如诺考达唑的存在下孵育重构的胚胎。 然后，重建的胚胎可能会产生一种或多种活的动物分娩。 本发明可用于生产转基因动物以及具有高遗传优点的非转基因植物。

公开(公告)号：US20060156424A1

公开(公告)日：2006-07-13

申请号：US11265113

申请日：2005-11-03

申请人： Keith Campbell ,  Ian Wilmut

发明人： Keith Campbell ,  Ian Wilmut

IPC分类号： A01K67/027

CPC分类号： C12N15/8771 ,  A61K35/54 ,  C12N15/8773

摘要： A method of reconstituting an animal embryo involves transferring the nucleus from a quiescent donor cell into a suitable recipient cell. The donor cell is quiescent, in that it is caused to exit from the growth and division cycle at G1 and to arrest in the G0 state. Nuclear transfer may take place by cell fusion. The reconstituted embryo may then give rise to one or more animals. The invention is useful in, the production of transgenic animals as well as non-transgenics of high genetic merit.

摘要翻译： 重建动物胚胎的方法涉及将细胞核从静止供体细胞转移到合适的受体细胞中。 供体细胞是静止的，因为它被导致在G1期的生长和分裂周期退出并且在G0状态下停止。 核转移可能通过细胞融合发生。 然后，重构的胚胎可以产生一种或多种动物。 本发明可用于生产转基因动物以及具有高遗传优点的非转基因植物。

公开(公告)号：US20070033664A1

公开(公告)日：2007-02-08

申请号：US11543786

申请日：2006-10-06

申请人： Keith Campbell ,  Ian Wilmut

发明人： Keith Campbell ,  Ian Wilmut

IPC分类号： A01K67/027 ,  C12N5/06

CPC分类号： C12N15/8771 ,  A61K35/54 ,  C12N15/8773

摘要： A method of reconstituting an animal embryo involves transferring the nucleus from a quiescent donor cell into a suitable recipient cell. The donor cell is quiescent, in that it is caused to exit from the growth and division cycle at G1 and to arrest in the G0 state. Nuclear transfer may take place by cell fusion. The reconstituted embryo may then give rise to one or more animals. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.

公开(公告)号：US20050034181A1

公开(公告)日：2005-02-10

申请号：US10914161

申请日：2004-08-10

申请人： Keith Campbell ,  Ian Wilmut

发明人： Keith Campbell ,  Ian Wilmut

IPC分类号： A01K67/02 ,  A61K35/54 ,  C12N5/10 ,  C12N15/02 ,  C12N15/873 ,  C12N15/877 ,  A01K67/027

CPC分类号： C12N15/8773 ,  A61K35/54 ,  C12N15/873 ,  C12N15/8771

摘要： A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at the time of transfer, so that the donor nucleus is kept exposed to the recipient cytoplasm for a period of time. The diploid nucleus can be donated by a cell in either the G0 or G1 phase of the cell cycle at the time of transfer. Subsequently, the reconstituted embryo is activated. Correct ploidy is maintained during activation, for example, by incubating the reconstituted embryo in the presence of a microtubule inhibitor such as nocodazole. The reconstituted embryo may then give rise to one or more live animal births. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.

公开(公告)号：US20060249818A1

公开(公告)日：2006-11-09

申请号：US11484075

申请日：2006-07-11

申请人： Brian Peterson ,  John Kirner ,  Scott Weigel ,  James MacDougall ,  Lisa Deis ,  Thomas Braymer ,  Keith Campbell ,  Martin Devenney ,  C. Ramberg ,  Konstantinos Chondroudis ,  Keith Cendak

发明人： Brian Peterson ,  John Kirner ,  Scott Weigel ,  James MacDougall ,  Lisa Deis ,  Thomas Braymer ,  Keith Campbell ,  Martin Devenney ,  C. Ramberg ,  Konstantinos Chondroudis ,  Keith Cendak

IPC分类号： H01B1/12 ,  H01B1/00

CPC分类号： H01L21/02126 ,  H01L21/02203 ,  H01L21/02216 ,  H01L21/02282 ,  H01L21/3122 ,  H01L21/31695

摘要： Low dielectric materials and films comprising same have been identified for improved performance when used as performance materials, for example, in interlevel dielectrics integrated circuits as well as methods for making same. In one aspect of the present invention, the performance of the dielectric material may be improved by controlling the weight percentage of ethylene oxide groups in the at least one porogen.

公开(公告)号：US20070157037A1

公开(公告)日：2007-07-05

申请号：US11323861

申请日：2005-12-30

申请人： Keith Campbell ,  Jeffery Franke ,  Donald Johnson ,  Brooks Johnston

发明人： Keith Campbell ,  Jeffery Franke ,  Donald Johnson ,  Brooks Johnston

IPC分类号： G06F1/00

CPC分类号： G06F1/206

摘要： An array of electronic devices includes at least one throttlable device in the array of devices and a management module connected to the at least one throttlable device for reducing heat effects to an overheating device from neighboring devices in the array of electronic devices device. The management module is configured to detect the overheating of the overheating device, detect at least one throttlable device neighboring the overheating device and in the array of devices, and throttle the at least one throttlable device. The array of devices are an array of blade servers within a blade center, and the management module is separate from the at least one throttlable device.

摘要翻译： 电子设备阵列包括装置阵列中的至少一个可节流装置和连接到该至少一个可节流装置的管理模块，用于减少来自电子装置装置阵列中的相邻装置的过热装置的热效应。 管理模块被配置为检测过热装置的过热，检测与过热装置和装置阵列相邻的至少一个可节流装置，并且对至少一个可节流装置进行节流。 所述设备阵列是刀片中心内的刀片服务器阵列，并且所述管理模块与所述至少一个可节流装置分开。

公开(公告)号：US20090137040A1

公开(公告)日：2009-05-28

申请号：US12212557

申请日：2008-09-17

申请人： Jose Cibelli ,  Michael West ,  Keith Campbell

发明人： Jose Cibelli ,  Michael West ,  Keith Campbell

IPC分类号： C12N5/08

CPC分类号： C12N5/16 ,  C12N15/873 ,  C12N15/8776 ,  C12N2517/04 ,  C12N2517/10

摘要： Activated human embryos produced by therapeutic cloning can give rise to human totipotent and pluripotent stem cells from which autologous cells for transplantation therapy are derived. The present invention provides methods for producing activated human embryos that can be used to generate totipotent and pluripotent stem cells from which autologous cells and tissues suitable for transplantation can be derived. In one embodiment, the invention provides methods for producing activated human embryos by parthenogenesis; in another embodiment, the invention provides methods for producing activated human embryos by somatic cell nuclear transfer whereby the genetic material of a differentiated human donor cell is reprogrammed to form a diploid human pronucleus capable of directing a cell to generate the stem cells from which autologous, isogenic cells for transplantation therapy are derived. The ability to create autologous human embryos represents a critical step towards generating immune-compatible stem cells that can be used to overcome the problem of immune rejection in regenerative medicine. The activated human embryos produced by the present invention also provide model systems for identifying and analyzing the molecular mechanisms of epigenetic imprinting and the genetic regulation of embryogenesis and development.

摘要翻译： 通过治疗性克隆产生的活化的人类胚胎可以产生源自移植治疗的自体细胞的人类全能干细胞和多能干细胞。 本发明提供了用于产生活化的人胚胎的方法，所述活化的人胚胎可用于产生能够产生适合于移植的自体细胞和组织的全能干细胞和多能干细胞。 在一个实施方案中，本发明提供了通过孤雌生殖产生活化的人胚胎的方法; 在另一个实施方案中，本发明提供了通过体细胞核转移产生活化的人胚胎的方法，由此将分化的人供体细胞的遗传物质重新编程以形成能引导细胞产生干细胞的二倍体人原核，其中自体， 衍生出用于移植治疗的同基因细胞。 创建自体人类胚胎的能力代表了产生免疫相容性干细胞的关键步骤，可用于克服再生医学中的免疫排斥问题。 本发明生产的活化人胚胎还提供了用于鉴定和分析表观遗传印记的分子机制和胚胎发生和发育的遗传调控的模型系统。

公开(公告)号：US20130102073A1

公开(公告)日：2013-04-25

申请号：US13653094

申请日：2012-10-16

申请人： Jose Cibelli ,  Michael West ,  Keith Campbell

发明人： Jose Cibelli ,  Michael West ,  Keith Campbell

IPC分类号： C12N5/16

CPC分类号： C12N5/16 ,  C12N15/873 ,  C12N15/8776 ,  C12N2517/04 ,  C12N2517/10

摘要： Activated human embryos produced by therapeutic cloning can give rise to human totipotent and pluripotent stem cells from which autologous cells for transplantation therapy are derived. The present invention provides methods for producing activated human embryos that can be used to generate totipotent and pluripotent stem cells from which autologous cells and tissues suitable for transplantation can be derived. The ability to create autologous human embryos represents a critical step towards generating immune-compatible stem cells that can be used to overcome the problem of immune rejection in regenerative medicine. The activated human embryos produced by the present invention also provide model systems for identifying and analyzing the molecular mechanisms of epigenetic imprinting and the genetic regulation of embryogenesis and development.

摘要翻译： 通过治疗性克隆产生的活化的人类胚胎可以产生源自移植治疗的自体细胞的人类全能干细胞和多能干细胞。 本发明提供了用于产生活化的人胚胎的方法，所述活化的人胚胎可用于产生能够产生适合于移植的自体细胞和组织的全能干细胞和多能干细胞。 创建自体人类胚胎的能力代表了产生免疫相容性干细胞的关键步骤，可用于克服再生医学中的免疫排斥问题。 本发明生产的活化人胚胎还提供了用于鉴定和分析表观遗传印记的分子机制和胚胎发生和发育的遗传调控的模型系统。

公开(公告)号：US20080267930A1

公开(公告)日：2008-10-30

申请号：US11996434

申请日：2006-07-21

申请人： Andrew Johnson ,  Keith Campbell ,  Ramiro Alberio

发明人： Andrew Johnson ,  Keith Campbell ,  Ramiro Alberio

IPC分类号： A61K35/12 ,  C12N15/06 ,  C12N5/22 ,  A61P43/00 ,  C12N1/00

CPC分类号： C12N5/16 ,  A61K35/60 ,  A61K35/65 ,  C07K14/4702 ,  C12N5/0696 ,  C12N15/8773 ,  C12N2501/605 ,  C12N2502/04 ,  C12N2506/00 ,  C12N2517/10

摘要： A method of producing a reprogrammed cell or reprogrammed cell nucleus, comprising exposing a differentiated cell, or the nucleus of a differentiated cell to a cell or cell extract thereof derived from an oocyte, egg, ovary or early embryo of a cold blooded vertebrate, wherein the cold blooded vertebrate has one or more of the following properties: (i) a primitive vertebrate body plan including laterally projecting ribs and/or spinal projections; (ii) germ cells which do not contain germ plasm; and/or (iii) the oocyte, egg, ovary or early embryo cell or cell from which the cell extract is derived, expresses a highly conserved form of Oct-4 and/or nanog. There is also provided uses of the reprogrammed cells.

摘要翻译： 一种生产重编程细胞或重编程细胞核的方法，包括将分化的细胞或分化细胞的核暴露于衍生自冷血脊椎动物的卵母细胞，卵，卵巢或早期胚胎的细胞或细胞提取物中，其中 冷血脊椎动物具有以下一个或多个性质：（i）包括横向突出的肋和/或脊柱突起的原始脊椎动物体平面图; （ii）不含生殖细胞的生殖细胞; 和/或（iii）来自细胞提取物的卵母细胞，卵，卵巢或早期胚胎细胞或细胞，表达高度保守形式的Oct-4和/或纳米。 还提供了重编程细胞的用途。