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公开(公告)号：US20080096244A1
公开(公告)日：2008-04-24
申请号：US11627256
申请日：2007-01-25
CPC分类号： C12N9/80
摘要： A D-aminoacylase having a high substrate specificity is provided. This D-aminoacylase can produce D-amino acids from N-acetyl-D,L-amino acids conveniently and efficiently at a low cost. A D-aminoacylase produced by a microorganism of genus Defluvibacter; which acts on a N-acetyl-D-amino acid; which has a molecular weight (as determined by electrophoresis) of about 55,000 daltons, and an isoelectric point (as determined by two-dimensional electrophoresis for denatured system) of 5.3; which acts on N-acetyl-D-valine, N-acetyl-D-leucine, and the like, but not on N-acetyl-L-valine, N-acetyl-L-leucine, and the like; which has an optimal temperature of 37° C. (pH 8) and an optimal pH value of 8 to 8.5 at 37° C.; and whose activity is inhibited by Mn2+, Co2+, Ni2+, and Zn2+ each at 1 mmol/L, and by dithiothreitol, 2-mercaptoethanol, o-phenanthroline, and L-cysteine each at 5 mmol/L.
摘要翻译： 提供了具有高底物特异性的D-氨基酸酰化酶。 该D-氨基酸酶可以以低成本方便且有效地从N-乙酰基-D，L-氨基酸产生D-氨基酸。 由去纤维杆菌属的微生物产生的D-氨基酸酰化酶; 其作用于N-乙酰基-D-氨基酸; 其具有约55,000道尔顿的分子量（通过电泳测定）和5.3的等电点（通过用于变性体系的二维电泳测定）; 其作用于N-乙酰基-D-缬氨酸，N-乙酰基-D-亮氨酸等，但不适用于N-乙酰基-L-缬氨酸，N-乙酰基-L-亮氨酸等; 其最适温度为37℃（pH8），最适pH值为8〜8.5，在37℃下。 并且其活性受到Mn 2+，Co 2+，Ni 2+，Zn 2+，Zn 2+， 每个为1mmol / L，二硫苏糖醇，2-巯基乙醇，邻菲咯啉和L-半胱氨酸各为5mmol / L。
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公开(公告)号：US20060172375A1
公开(公告)日：2006-08-03
申请号：US10539281
申请日：2003-12-17
CPC分类号： C12N9/80
摘要： A D-aminoacylase having a high substrate specificity is provided. This D-aminoacylase can produce D-amino acids from N-acetyl-D,L-amino acids conveniently and efficiently at a low cost. A D-aminoacylase produced by a microorganism of genus Defluvibacter; which acts on a N-acetyl-D-amino acid; which has a molecular weight (as determined by electrophoresis) of about 55,000 daltons, and an isoelectric point (as determined by two-dimensional electrophoresis for denatured system) of 5.3; which acts on N-acetyl-D-valine, N-acetyl-D-leucine, and the like, but not on N-acetyl-L-valine, N-acetyl-L-leucine, and the like; which has an optimal temperature of 37° C. (pH 8) and an optimal pH value of 8 to 8.5 at 37° C.; and whose activity is inhibited by Mn2+, Co2+, Ni2+, and Zn2+ each at 1 mmol/L, and by dithiothreitol, 2-mercaptoethanol, o-phenanthroline, and L-cysteine each at 5 mmol/L.
摘要翻译： 提供了具有高底物特异性的D-氨基酸酰化酶。 该D-氨基酸酶可以以低成本方便且有效地从N-乙酰基-D，L-氨基酸产生D-氨基酸。 由去纤维杆菌属的微生物产生的D-氨基酸酰化酶; 其作用于N-乙酰基-D-氨基酸; 其具有约55,000道尔顿的分子量（通过电泳测定）和5.3的等电点（通过用于变性体系的二维电泳测定）; 其作用于N-乙酰基-D-缬氨酸，N-乙酰基-D-亮氨酸等，但不适用于N-乙酰基-L-缬氨酸，N-乙酰基-L-亮氨酸等; 其最适温度为37℃（pH8），最适pH值为8〜8.5，在37℃下。 并且其活性受到Mn 2+，Co 2+，Ni 2+，Zn 2+，Zn 2+， 每个为1mmol / L，二硫苏糖醇，2-巯基乙醇，邻菲咯啉和L-半胱氨酸各为5mmol / L。
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公开(公告)号：US20090215118A1
公开(公告)日：2009-08-27
申请号：US12397879
申请日：2009-03-04
CPC分类号： C12N9/80
摘要： A D-aminoacylase having a high substrate specificity is provided. This D-aminoacylase can produce D-amino acids from N-acetyl-D,L-amino acids conveniently and efficiently at a low cost.A D-aminoacylase produced by a microorganism of genus Defluvibacter; which acts on a N-acetyl-D-amino acid; which has a molecular weight (as determined by electrophoresis) of about 55,000 daltons, and an isoelectric point (as determined by two-dimensional electrophoresis for denatured system) of 5.3; which acts on N-acetyl-D-valine, N-acetyl-D-leucine, and the like, but not on N-acetyl-L-valine, N-acetyl-L-leucine, and the like; which has an optimal temperature of 37° C. (pH 8) and an optimal pH value of 8 to 8.5 at 37° C.; and whose activity is inhibited by Mn2+, Co2+, Ni2+, and Zn2+ each at 1 mmol/L, and by dithiothreitol, 2-mercaptoethanol, o-phenanthroline, and L-cysteine each at 5 mmol/L.
摘要翻译： 提供了具有高底物特异性的D-氨基酸酰化酶。 该D-氨基酸酶可以以低成本方便且有效地从N-乙酰基-D，L-氨基酸产生D-氨基酸。 由去纤维杆菌属的微生物产生的D-氨基酸酰化酶; 其作用于N-乙酰基-D-氨基酸; 其具有约55,000道尔顿的分子量（通过电泳测定）和5.3的等电点（通过用于变性体系的二维电泳测定）; 其作用于N-乙酰基-D-缬氨酸，N-乙酰基-D-亮氨酸等，但不适用于N-乙酰基-L-缬氨酸，N-乙酰基-L-亮氨酸等; 其最适温度为37℃（pH8），最适pH值为8〜8.5，在37℃下。 Mn2 +，Co2 +，Ni2 +和Zn2 +各自以1 mmol / L的速度抑制其活性，二硫苏糖醇，2-巯基乙醇，邻菲咯啉和L-半胱氨酸各自以5 mmol / L的速度进行抑制。
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4.发明申请公开(公告)号：US20110244530A1
公开(公告)日：2011-10-06
申请号：US12989728
申请日：2009-05-07
申请人： Atsushi Toda , Sachio Iwai , Yoshiaki Nishiya , Takeshi Miyata , Aya Oosato , Shinya Kumagai , Toshihide Yamada
发明人： Atsushi Toda , Sachio Iwai , Yoshiaki Nishiya , Takeshi Miyata , Aya Oosato , Shinya Kumagai , Toshihide Yamada
CPC分类号： C12N9/80 , A23L33/175 , C12P13/04 , C12P13/06 , C12P41/007
摘要： The present invention provides an L-aminoacylase which is able to produce L-tert-leucine being useful as an intermediate for pharmaceuticals.A protein which is characterized in being represented by any of the following (a) to (d): (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; and (d) a protein which consists of an amino acid sequence where one or several amino acid (s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity.
摘要翻译： 本发明提供能够产生L-叔亮氨酸的L-氨基酸酰基酶，其可用作药物的中间体。 特征在于以下（a）〜（d）中任一项所示的蛋白质：（a）由由SEQ ID No：1所示的核酸序列组成的基因编码的蛋白质; （b）由SEQ ID No：2所示的氨基酸序列组成的蛋白质; （c）由严格条件下与SEQ ID No：1所示核酸序列互补且具有L-琥珀酰氨基酰化酶活性的核酸序列杂交的多核苷酸编码的蛋白质; 和（d）由氨基酸序列组成的蛋白质，其中一个或几个氨基酸被取代，缺失，插入和/或添加在由SEQ ID No：2所示的氨基酸序列组成的蛋白质中 并具有L-琥珀酰氨基酰化酶活性。
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5.发明授权公开(公告)号：US08728771B2
公开(公告)日：2014-05-20
申请号：US13139205
申请日：2009-12-10
申请人： Atsushi Toda , Sachio Iwai , Yoshiaki Nishiya , Shinya Kumagai
发明人： Atsushi Toda , Sachio Iwai , Yoshiaki Nishiya , Shinya Kumagai
CPC分类号： C12N9/80 , C12P13/04 , C12P13/06 , C12P13/222 , C12P41/007
摘要： The present invention provides a L-succinylacylase consisting of: (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; or (d) a protein which consists of an amino acid sequence where one or several amino acid(s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity. This enzyme is able to produce a sterically bulky unnatural amino acid such as L-tert-leucine etc. which is useful as an intermediate for pharmaceuticals.
摘要翻译： 本发明提供了一种L-琥珀酰基酰化酶，其由以下组成：（a）由由SEQ ID No：1所示的核酸序列组成的基因编码的蛋白质; （b）由SEQ ID No：2所示的氨基酸序列组成的蛋白质; （c）由严格条件下与SEQ ID No：1所示核酸序列互补且具有L-琥珀酰氨基酰化酶活性的核酸序列杂交的多核苷酸编码的蛋白质; 或（d）由氨基酸序列组成的蛋白质，其中一个或多个氨基酸被取代，缺失，插入和/或添加在由SEQ ID No：2所示的氨基酸序列组成的蛋白质中 并具有L-琥珀酰氨基酰化酶活性。 该酶能够产生空间庞大的非天然氨基酸如L-叔亮氨酸等，其可用作药物的中间体。
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公开(公告)号：US20080108227A1
公开(公告)日：2008-05-08
申请号：US11878691
申请日：2007-07-26
IPC分类号： H01L21/28
CPC分类号： H01L29/7613 , B82Y10/00 , H01L29/66439 , H01L49/006 , Y10S438/962 , Y10S977/773
摘要： The present invention provides a method for production of a single electron semiconductor element (SET) in which a quantum dot is selectively arranged in a nano gap between fine electrodes, whereby the product yield is significantly improved, leading to excellent practical applicability. The method for production of SET of the present invention is characterized in that a solution containing ferritin including a metal or semiconductor particle therein, and a nonionic surfactant is dropped on a substrate having a source electrode and a drain electrode formed by laminating a titanium film and a film of a metal other than titanium, whereby the ferritin is selectively arranged in a nano gap between the source electrode/drain electrode. In the method for production of SET of the present invention, the metal or semiconductor particle can be fixed as a quantum dot at a suitable position in the nano gap between the source electrode/drain electrode following decomposition of ferritin, and in addition, formation of unnecessary quantum dot can be suppressed.
摘要翻译： 本发明提供一种单电子半导体元件（SET）的制造方法，其中量子点选择性地排列在细电极之间的纳米间隙中，从而显着提高了产品产率，从而实现了优异的实用性。 本发明的SET的制造方法的特征在于，将含有金属或半导体粒子的铁蛋白和非离子表面活性剂的溶液滴落在具有源极电极和漏电极的基板上，所述源极和漏极通过层叠钛膜和 除钛以外的金属的膜，由此铁素体选择性地排列在源电极/漏电极之间的纳米间隙中。 在本发明的SET的制造方法中，金属或半导体粒子可以在铁蛋白分解后的源电极/漏电极之间的纳米间隙的适当位置作为量子点固定，另外，形成 可以抑制不必要的量子点。
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7.发明授权Extruded foamed polyolefin resin carrier for microorganisms in a fluidized bed 失效用于流化床微生物的挤出发泡聚烯烃树脂载体
公开(公告)号：US5962309A
公开(公告)日：1999-10-05
申请号：US6215
申请日：1998-01-13
申请人： Shinya Kumagai , Kazuyasu Tanaka
发明人： Shinya Kumagai , Kazuyasu Tanaka
摘要： A microorganism holding carrier for a fluidized bed is provided that includes an extruded foamed article having continuous cells and independent cells. The article is composed of a polyolefin resin as a main component. The continuous cells include open-cells which are opened to at least two portions of a surface of the article and a semi-open cell which are opened to only one portion of the surface of the article. A ratio of the total volume of the continuous cells to the total volume of the extruded foamed article (a volume ratio of the continuous cells) falls within a range of from 20% to 70%. Further, a ratio of the total volume of the open-cells to the total volume of the continuous cells (a volume ratio of open-cells) is 20% or more. The article may have an apparent density of 0.90 to 1.80 g/cm.sup.3 and an apparent volume from 2.0.times.10.sup.-3 to 5.0 cm.sup.3, and be in the shape of a column, or a tube having an outer diameter of 2 to 20 mm and a thickness of 5 to 30% of the outer diameter. During foam-extruding, a cylinder temperature of 180.degree. C. to 250.degree. C. and a die temperature of 200.degree. C. to 250.degree. C. are preferred. The polyolefin resin may include additives such as an inorganic filler, a foaming agent, and a blowing assistant for controlling foaming temperature of the foaming agent.
摘要翻译： 提供一种用于流化床的微生物保持载体，其包括具有连续细胞和独立细胞的挤出发泡制品。 该制品由聚烯烃树脂作为主要成分组成。 连续细胞包括对制品表面的至少两个部分开放的开放细胞和仅打开制品表面的一部分的半开放细胞。 连续细胞的总体积与挤出发泡制品的总体积（连续细胞的体积比）的比率在20％至70％的范围内。 此外，开放细胞的总体积与连续细胞的总体积的比率（开放细胞的体积比）为20％以上。 制品的表观密度可以为0.90〜1.80g / cm 3，表观体积为2.0×10 -3〜5.0cm 3，呈柱状或外径为2〜20mm的管， 厚度为外径的5〜30％。 在发泡挤出过程中，优选气缸温度为180℃至250℃，模具温度为200℃至250℃。 聚烯烃树脂可以包括添加剂如无机填料，发泡剂和用于控制发泡剂的发泡温度的吹塑助剂。
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8.发明授权公开(公告)号：US07919596B2
公开(公告)日：2011-04-05
申请号：US12320556
申请日：2009-01-29
IPC分类号： C07K14/00 , H01L21/00 , H01L21/314 , H01L21/471
摘要： To provide a method of arranging ferritin by which a high rate of the number of the molecular film spots on which sole ferritin molecule was arranged in effect, with respect to total number of the molecular film spots provided for arranging ferritin (sole arrangement rate) is achieved is objected to. Specifically, in Fer8 ferritin having a sequence excluding 7 amino acids of from the second to the eighth, from an amino acid sequence (Fer0 sequence) translated from a naturally occurring DNA sequence, lysine at position 91 is substituted with glutamic acid.
摘要翻译： 为了提供一种安排铁蛋白的方法，相对于提供用于安排铁蛋白的总分子膜点数（单独排列速率），通过该铁蛋白排列高分子膜斑点的数量，单个铁蛋白分子排列有效的方法是： 实现是反对的。 具体而言，在来自第二至第八的7个氨基酸序列的Fer8铁蛋白中，从天然存在的DNA序列翻译的氨基酸序列（Fer0序列）中，91位的赖氨酸被谷氨酸取代。
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公开(公告)号：US07419849B2
公开(公告)日：2008-09-02
申请号：US11878691
申请日：2007-07-26
IPC分类号： H01L51/40
CPC分类号： H01L29/7613 , B82Y10/00 , H01L29/66439 , H01L49/006 , Y10S438/962 , Y10S977/773
摘要： The present invention provides a method for production of a single electron semiconductor element (SET) in which a quantum dot is selectively arranged in a nano gap between fine electrodes, whereby the product yield is significantly improved, leading to excellent practical applicability. The method for production of SET of the present invention is characterized in that a solution containing ferritin including a metal or semiconductor particle therein, and a nonionic surfactant is dropped on a substrate having a source electrode and a drain electrode formed by laminating a titanium film and a film of a metal other than titanium, whereby the ferritin is selectively arranged in a nano gap between the source electrode/drain electrode.
摘要翻译： 本发明提供一种单电子半导体元件（SET）的制造方法，其中量子点选择性地排列在细电极之间的纳米间隙中，从而显着提高了产品产率，从而实现了优异的实用性。 本发明的SET的制造方法的特征在于，将含有金属或半导体粒子的铁蛋白和非离子表面活性剂的溶液滴落在具有源极电极和漏电极的基板上，所述源极和漏极通过层叠钛膜和 除钛以外的金属的膜，由此铁素体选择性地排列在源电极/漏电极之间的纳米间隙中。
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10.发明授权公开(公告)号：US08712297B2
公开(公告)日：2014-04-29
申请号：US13271716
申请日：2011-10-12
申请人： Shiro Ohashi , Hiroshi Nishikawa , Hiroki Shiba , Shinya Kumagai
发明人： Shiro Ohashi , Hiroshi Nishikawa , Hiroki Shiba , Shinya Kumagai
IPC分类号： G03G15/16
CPC分类号： G03G15/0136 , G03G15/0189
摘要： A transfer device includes an endless member caused to circulate by a driving unit, plural transfer members coming in contact with a rear surface of the endless member and transferring toner images on image holding members to the endless member, a support member movably supporting a specific transfer member of the transfer members located at a position which is more upstream in a circulation direction of the endless member than a contact position where a recording medium comes in contact with the endless member and which is closest to the contact position, a regulation member regulating the movement of the specific transfer member in a state where the specific transfer member is disposed at a position where the toner image formed on the corresponding image holding member is transferred to the endless member, and a first impelling member impelling the support member to the regulation member.
摘要翻译： 传送装置包括环形构件，其通过驱动单元循环，多个传送构件与环形构件的后表面接触并将图像保持构件上的调色剂图像转印到环形构件;可移动地支撑特定转印件的支撑构件 传送构件的构件位于环形构件的循环方向上比在记录介质与环形构件接触并且最接近接触位置的接触位置更上游的位置处的调节构件，调节构件 特定转印部件在将特定转印部件配置在形成于对应的图像保持部件上的调色剂图像的位置被转印到环状部件的位置的状态下的移动，以及将支撑部件推压到限制部件的第一推动部件 。
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