公开(公告)号：US5720923A

公开(公告)日：1998-02-24

申请号：US299033

申请日：1994-08-31

申请人： Lawrence A. Haff ,  Enrico Picozza ,  Will Bloch ,  Robert Ragusa ,  Joseph DiCesare ,  David Tracy ,  Paul Saviano ,  Timothy M. Woudenberg

发明人： Lawrence A. Haff ,  Enrico Picozza ,  Will Bloch ,  Robert Ragusa ,  Joseph DiCesare ,  David Tracy ,  Paul Saviano ,  Timothy M. Woudenberg

IPC分类号： C12N15/09 ,  B01J19/00 ,  B01L7/00 ,  C12M1/00 ,  C12Q1/68 ,  C40B40/06 ,  C40B60/14 ,  G01N35/00 ,  C12M1/40 ,  C12P19/34

CPC分类号： B01L7/525 ,  B01J19/0046 ,  B01L7/52 ,  B01L9/065 ,  B01J2219/00495 ,  B01J2219/00722 ,  B01L2300/1883 ,  C12Q1/686 ,  C40B40/06 ,  C40B60/14 ,  G01N2035/00237 ,  Y10T436/116664

摘要： Apparatus and method for performing a nucleic acid amplification reaction and preferably a polymerase chain reaction (PCR) in a reaction mixture in at least one capillary tube. Several different embodiments are disclosed. One embodiment cycles a sample through a capillary tube loop passing through two thermostatted fluid baths. Another embodiment has the capillary tube routed alternatingly between two heat exchangers to that the sample makes only one pass through the tube. Other embodiments maintain the heat exchangers stationary and translate the samples between them. Still further embodiments maintain the samples stationary and either automatically translate or rotate the heat exchangers past the samples contained within the capillary tubes to perform the thermal cycles necessary for the amplification reaction.

公开(公告)号：US6033880A

公开(公告)日：2000-03-07

申请号：US27989

申请日：1998-02-23

申请人： Lawrence A. Haff ,  Enrico Picozza ,  Will Bloch ,  Robert Ragusa ,  Joseph DiCesare ,  David Tracy ,  Paul Saviano ,  Timothy M. Woudenberg ,  Richard W. Noreiks

发明人： Lawrence A. Haff ,  Enrico Picozza ,  Will Bloch ,  Robert Ragusa ,  Joseph DiCesare ,  David Tracy ,  Paul Saviano ,  Timothy M. Woudenberg ,  Richard W. Noreiks

IPC分类号： C12N15/09 ,  B01J19/00 ,  B01L7/00 ,  C12M1/00 ,  C12Q1/68 ,  C40B40/06 ,  C40B60/14 ,  G01N35/00 ,  C12P19/34

CPC分类号： B01L7/525 ,  B01J19/0046 ,  B01L7/52 ,  B01L9/065 ,  B01J2219/00495 ,  B01J2219/00722 ,  B01L2300/1883 ,  C12Q1/686 ,  C40B40/06 ,  C40B60/14 ,  G01N2035/00237 ,  Y10T436/116664

摘要： Apparatus and method for performing a nucleic acid amplification reaction and preferably a polymerase chain reaction (PCR) in a reaction mixture in at least one capillary tube. Several different embodiments are disclosed. One embodiment cycles a sample through a capillary tube loop passing through two thermostatted fluid baths. Another embodiment has the capillary tube routed alternatingly between two heat exchangers to that the sample makes only one pass through the tube. Other embodiments maintain the heat exchangers stationary and translate the samples between them. Still further embodiments maintain the samples stationary and either automatically translate or rotate the heat exchangers past the samples contained within the capillary tubes to perform the thermal cycles necessary for the amplification reaction.

摘要翻译： 用于在至少一个毛细管中的反应混合物中进行核酸扩增反应和优选聚合酶链式反应（PCR）的装置和方法。 公开了几个不同的实施例。 一个实施例通过穿过两个恒温流体浴的毛细管回路循环样品。 另一个实施例中，毛细管在两个热交换器之间交替布置，使得样品仅穿过管中一次。 其他实施例保持热交换器平稳，并在它们之间平移样品。 另外的实施例保持样品静止，并且自动地将热交换器平移或旋转通过毛细管内包含的样品，以进行扩增反应所需的热循环。

公开(公告)号：US20060003366A1

公开(公告)日：2006-01-05

申请号：US11150492

申请日：2005-06-10

申请人： Joseph DiCesare

发明人： Joseph DiCesare

IPC分类号： C12Q1/68 ,  C12M1/34 ,  G01N33/551

CPC分类号： G01N33/54353 ,  C12N15/1034 ,  C12Q1/6834 ,  C12Q1/6837 ,  G01N33/54346 ,  G01N2035/00158 ,  C12Q2537/125 ,  C12Q2563/185 ,  C12Q2563/167 ,  C12Q2525/197 ,  C12Q2565/627

摘要： High density multiplexing assays for proteins, nucleic acids, and other molecules are described based upon using mass spectrometry detection. A solid-state support assay format is utilized, whereby, the bead carries both a coding molecule to track the sample or analyte and the molecule(s) necessary to perform an affinity-based assay. After the necessary reactions are complete, all of the components, e.g., coding molecule, binding molecule, analyte, and/or label are dissociated for analysis in a mass spectrometer. Various means of performing detection for the assays include different types of mass spectrometers with different types of sampling systems, including MALDI-TOF and ESI.

摘要翻译： 基于使用质谱检测来描述蛋白质，核酸和其他分子的高密度多重测定。 使用固态支持测定形式，由此，珠粒携带编码分子以跟踪样品或分析物以及进行基于亲和性测定所必需的分子。 在必要的反应完成后，所有组分，例如编码分子，结合分子，分析物和/或标记物在质谱仪中解离以进行分析。 进行检测的各种方法包括不同类型的具有不同类型采样系统的质谱仪，包括MALDI-TOF和ESI。

公开(公告)号：US20050274886A1

公开(公告)日：2005-12-15

申请号：US11207393

申请日：2005-08-19

申请人： Joseph DiCesare

发明人： Joseph DiCesare

IPC分类号： B01D59/44 ,  B01L3/00 ,  H01J49/04 ,  H01J49/16

CPC分类号： G01N1/2813 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2300/0819 ,  B01L2300/161 ,  G01N2001/045 ,  G01N2001/282 ,  H01J49/0418 ,  H01J49/164 ,  Y10S436/809 ,  Y10T436/25

摘要： A method of making a sample plate for mass spectrometric analysis of a specimen is provided comprising applying a mask to a substrate to form at least one sample site. The sample plate comprises a substrate having an electrically conductive surface, a mask applied to the electrically conductive surface. The mask has a rougher surface than the substrate. The sample site comprises a central portion formed from the electrically conductive surface and a marginal portion formed from the mask where the marginal portion is more hydrophobic than the central portion.

公开(公告)号：US20050029446A1

公开(公告)日：2005-02-10

申请号：US10881913

申请日：2004-06-30

申请人： Joseph DiCesare

发明人： Joseph DiCesare

IPC分类号： B01D59/44 ,  B01L3/00 ,  H01J49/04 ,  H01J49/16 ,  H01J49/00

CPC分类号： G01N1/2813 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2300/0819 ,  B01L2300/161 ,  G01N2001/045 ,  G01N2001/282 ,  H01J49/0418 ,  H01J49/164 ,  Y10S436/809 ,  Y10T436/25

摘要： A method of making a sample plate for mass spectrometric analysis of a specimen is provided comprising applying a mask to a substrate to form at least one sample site. The sample plate comprises a substrate having an electrically conductive surface, a mask applied to the electrically conductive surface. The mask has a rougher surface than the substrate. The sample site comprises a central portion formed from the electrically conductive surface and a marginal portion formed from the mask where the marginal portion is more hydrophobic than the central portion.

摘要翻译： 提供了制备用于样品的质谱分析的样品板的方法，包括将掩模施加到基底以形成至少一个样品位点。 样品板包括具有导电表面的基底，施加到导电表面的掩模。 该掩模具有比基底更粗糙的表面。 样品位置包括由导电表面形成的中心部分和由掩模形成的边缘部分，其边缘部分比中心部分更疏水。