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    IKB kinase, subunits thereof, and methods of using same
    9.
    发明申请
    IKB kinase, subunits thereof, and methods of using same 审中-公开
    IKB激酶，其亚单位及其使用方法

    公开(公告)号：US20100055714A1

    公开(公告)日：2010-03-04

    申请号：US11982034

    申请日：2007-10-31

    申请人： Michael Karin ,  Joseph A. DiDonato ,  David M. Rothwarf ,  Makio Hayakawa ,  Ebrahim Zandi

    发明人： Michael Karin ,  Joseph A. DiDonato ,  David M. Rothwarf ,  Makio Hayakawa ,  Ebrahim Zandi

    IPC分类号： G01N33/53 ,  C07K16/18 ,  C12N5/16 ,  C12N9/12 ,  C12N5/071

    CPC分类号： G01N33/6842 ,  A61K38/00 ,  C07K16/40 ,  C12N9/1205 ,  C12Q1/48 ,  C12Y207/1101 ,  G01N33/68 ,  G01N2333/9121

    摘要： The present invention provides an isolated nucleic acid molecules encoding IκB kinase (IKK) catalytic subunit polypeptides, which are associated with an IKK serine protein kinase that phosphorylates a protein (IκB) that inhibits the activity of the NF-κB transcription factor, vectors comprising such nucleic acid molecules and host cells containing such vectors. In addition, the invention provides nucleotide sequences that can bind to a nucleic acid molecule of the invention, such nucleotide sequences being useful as probes or as antisense molecules. The invention also provides isolated IKK catalytic subunits, which can phosphorylate an IκB protein, and peptide portions of such IKK subunit. In addition, the invention provides anti-IKK antibodies, which specifically bind to an IKK complex or an IKK catalytic subunit, and IKK-binding fragments of such antibodies. The invention further provides methods of substantially purifying an IKK complex, methods of identifying an agent that can alter the association of an IKK complex or an IKK catalytic subunit with a second protein, and methods of identifying proteins that can interact with an IKK complex or an IKK catalytic subunit.

    摘要翻译： 本发明提供了编码IκB激酶（IKK）催化亚单位多肽的分离的核酸分子，其与IKK丝氨酸蛋白激酶相关联，其使磷酸化抑制NF- 转录因子，包含这种核酸分子的载体和含有这些载体的宿主细胞。 此外，本发明提供可结合本发明的核酸分子的核苷酸序列，该核苷酸序列可用作探针或作为反义分子。 本发明还提供分离的IKK催化亚单位，其可以磷酸化IκB蛋白和这种IKK亚基的肽部分。 此外，本发明提供了特异性结合IKK复合物或IKK催化亚单位的抗IKK抗体以及这种抗体的IKK结合片段。 本发明还提供了基本上纯化IKK复合物的方法，鉴定可以改变IKK复合物或IKK催化亚基与第二种蛋白质的缔合的试剂的方法，以及鉴定可与IKK复合物或 IKK催化亚单位。

    IKB kinase and methods of using same
    10.
    发明授权
    IKB kinase and methods of using same 失效
    IKB激酶及其使用方法

    公开(公告)号：US06268194B1

    公开(公告)日：2001-07-31

    申请号：US08810131

    申请日：1997-02-25

    申请人： Michael Karin ,  Joseph A. DiDonato ,  David M. Rothwarf ,  Makio Hayakawa ,  Ebrahim Zandi

    发明人： Michael Karin ,  Joseph A. DiDonato ,  David M. Rothwarf ,  Makio Hayakawa ,  Ebrahim Zandi

    IPC分类号： C12N912

    CPC分类号： G01N33/6842 ,  A61K38/00 ,  C07K16/40 ,  C12N9/1205 ,  C12Q1/48 ,  C12Y207/1101 ,  G01N33/68 ,  G01N2333/9121

    摘要： The present invention provides a substantially purified nucleic acid molecule encoding a serine protein kinase (I&kgr;B kinase) that phosphorylates a protein (I&kgr;B) that inhibits the activity of the NF-&kgr;B transcription factor, vectors comprising such a nucleic acid molecule and host cells containing such vectors. In addition, the invention provides nucleotide sequences that can bind to a nucleic acid molecule of the invention, such nucleotide sequences being useful as probes or as antisense molecules. The invention also provides a substantially purified I&kgr;B kinase, which is a polypeptide that can phosphorylate an I&kgr;B protein, and peptide portions of the I&kgr;B kinase. In addition, the invention provides anti-I&kgr;B kinase antibodies, which specifically bind to an I&kgr;B kinase, and I&kgr;B kinase-binding fragments of such antibodies. The invention further provides methods of substantially purifying an I&kgr;B kinase, methods of identifying an agent that can alter the association of an I&kgr;B kinase with a second protein, and methods of identifying proteins that can interact with an I&kgr;B kinase.

    摘要翻译： 本发明提供了编码丝氨酸蛋白激酶（IkappaB激酶）的基本上纯化的核酸分子，其磷酸化抑制NF-κB转录因子的活性的蛋白质（IkappaB），包含这种核酸分子的载体和含有这种核酸分子的宿主细胞 向量 此外，本发明提供可结合本发明的核酸分子的核苷酸序列，该核苷酸序列可用作探针或作为反义分子。 本发明还提供了基本上纯化的IkappaB激酶，其是能够磷酸化IkappaB蛋白的多肽和IkappaB激酶的肽部分。 此外，本发明提供了抗IkappaB激酶抗体，其特异性结合IkappaB激酶和这些抗体的IkappaB激酶结合片段。 本发明还提供了实质上纯化IkappaB激酶的方法，鉴定可以改变IkappaB激酶与第二种蛋白质的缔合的试剂的方法，以及鉴定可与IkappaB激酶相互作用的蛋白质的方法。