利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

106 条记录 (耗时 0.046 秒)

    Methods for the cryopreservation of cells
    1.
    发明授权
    Methods for the cryopreservation of cells 有权
    细胞冷冻保存方法

    公开(公告)号：US09538745B2

    公开(公告)日：2017-01-10

    申请号：US12226300

    申请日：2007-04-12

    申请人： Xiaoming He ,  Thomas L. Toth ,  Mehmet Toner ,  Jon Edd

    发明人： Xiaoming He ,  Thomas L. Toth ,  Mehmet Toner ,  Jon Edd

    IPC分类号： A01N1/02

    CPC分类号： A01N1/02 ,  A01N1/0221 ,  A01N1/0268 ,  A01N1/0278

    摘要： The present invention features novel methods for the cryopreservation of mammalian cell that combine the advantages of the slow-freezing and vitrification approaches while avoiding their shortcomings. Generally, the methods include the use of a capillary tube made of a thermally conductive wall material and a thin wall such that the ratio of the thermal conductivity of the wall material to the wall thickness is at least 1,000-500,000. The solution is then exposed to temperatures equal to or less than −80° C. and the vitrification solution containing the mammalian cells is cooled at a rate equal to or greater than 30,000-100,000,000° C./minute. The exposure of the capillary tube with a thermally conductive and thin wall allows for vitrification of the solution in the absence of ice formation. Cryoprotectants can also be added to the vitrification solution to further prevent ice formation.

    摘要翻译： 本发明的特征在于用于冷冻保存哺乳动物细胞的新方法，其结合了缓慢冷冻和玻璃化方法的优点，同时避免了它们的缺点。 通常，这些方法包括使用由导热壁材料制成的毛细管和薄壁，使得壁材料的导热率与壁厚之比至少为1,000-500,000。 然后将溶液暴露于等于或小于-80℃的温度，并将含有哺乳动物细胞的玻璃化溶液以等于或大于30,000-10000,000℃/分钟的速率冷却。 毛细管与导热和薄壁的接触允许在没有冰层形成的情况下玻璃化溶液。 也可以将冷冻保护剂加入到玻璃化溶液中以进一步防止冰层形成。

    Microscale and nanoscale structures for manipulating particles
    2.
    发明授权
    Microscale and nanoscale structures for manipulating particles 有权
    用于操纵颗粒的微尺度和纳米级结构

    公开(公告)号：US09422517B2

    公开(公告)日：2016-08-23

    申请号：US13812934

    申请日：2011-07-29

    申请人： Grace Chen ,  Fabio Fachin ,  Mehmet Toner ,  Brian Wardle

    发明人： Grace Chen ,  Fabio Fachin ,  Mehmet Toner ,  Brian Wardle

    IPC分类号： B07B9/00 ,  C12M1/12 ,  B01L3/00 ,  G01N15/14

    CPC分类号： C12M25/16 ,  B01L3/502707 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0896 ,  B01L2400/086 ,  G01N15/1404 ,  G01N2015/1413

    摘要： The devices and systems described herein include one or more fluid paths, e.g., channels, and one or more selectively permeable obstacles arranged in the fluid path(s), each including a plurality of aligned nanostructures, e.g., nanotubes or nanorods, defining an outer surface of the obstacle and an internal network of voids. The obstacle(s) can further include binding moieties applied to the outer surface and/or to the surfaces of the individual nanostructures within the obstacle(s). The devices can be manufactured by forming the dense groupings of nanostructures to extend outwards and upwards from a substrate; forming a fluidic channel, bonding the fluidic channel to the substrate; and optionally applying binding moieties to the obstacles. The devices can be used to manipulate cells within fluid samples.

    摘要翻译： 本文描述的装置和系统包括一个或多个流体路径，例如通道和布置在流体路径中的一个或多个选择性渗透的障碍物，每个包括多个对准的纳米结构，例如纳米管或纳米棒，限定外部 障碍物的表面和内部的空隙网络。 障碍物还可包括施加到障碍物内的各个纳米结构的外表面和/或表面的结合部分。 可以通过形成从衬底向外和向上延伸的纳米结构的致密分组来制造器件; 形成流体通道，将流体通道粘合到基底上; 并且任选地将结合部分施加到障碍物上。 这些装置可用于操纵流体样品中的细胞。

    Capturing particles
    3.
    发明授权
    Capturing particles 有权
    捕获颗粒

    公开(公告)号：US09128091B2

    公开(公告)日：2015-09-08

    申请号：US13121130

    申请日：2009-09-25

    申请人： Mehmet Toner ,  Shannon Stott ,  Chia-Hsien Hsu

    发明人： Mehmet Toner ,  Shannon Stott ,  Chia-Hsien Hsu

    IPC分类号： B01L3/00 ,  G01N33/569 ,  G01N33/543 ,  G01N33/574

    CPC分类号： G01N1/405 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0858 ,  B01L2400/0487 ,  B01L2400/086 ,  G01N33/54366 ,  G01N33/56966 ,  G01N33/57434

    摘要： Methods and systems capturing particles suspended in a fluid flowed through a micro-channel, can include flowing the fluid including the particles to be captured through a micro-channel and past a groove defined in a surface of a wall of the micro-channel such that flowing the fluid past the groove forms microvortices in the fluid; contacting at least some of the particles against an adherent disposed on one or more of walls of the microchannel after the microvortices form in the fluid; and capturing at least some of the particles contacting the adherent.

    摘要翻译： 捕获悬浮在流过微通道的流体中的颗粒的方法和系统可以包括使包含要捕获的颗粒的流体流过微通道并经过限定在微通道壁的表面中的凹槽，使得 使流体流过凹槽，形成微流体; 在微流体在流体中形成之后，使至少一些颗粒抵靠设置在微通道的一个或多个壁上的粘附剂; 并捕获与粘附剂接触的至少一些颗粒。