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公开(公告)号：US6119567A
公开(公告)日：2000-09-19
申请号：US891353
申请日：1997-07-10
CPC分类号： B26F3/08 , B26F1/3806
摘要： A method and preferred apparatus (10) to cut a preform material which is biodegradable and preferably dispersible in water, to produce a shaped article, is described. The cut is produced without producing harmful fumes or leaving a harmful residue and preferably without charring at the cut. Preferably, only small amounts of carbon dioxide and water are produced at the cut. Shaped articles which are in sheet or block form or which are for 2-D or 3-D shaped article formation can be produced.
摘要翻译： 描述了一种切割可生物降解且优选分散在水中的预成型材料的方法和优选设备（10），以生产成型制品。 切割产生而不产生有害烟雾或留下有害残留物，优选在切割时不起焦作用。 优选地，在切割处仅产生少量的二氧化碳和水。 可以制造片状或块状的成形制品或用于形成二维或三维形状的制品。
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公开(公告)号：US4624915A
公开(公告)日：1986-11-25
申请号：US513995
申请日：1983-07-14
CPC分类号： B07C5/342 , C12M47/04 , C12M47/06 , G01N2015/149
摘要： A method and apparatus for position selection of viable cells based upon differing chemical or physical properties or dynamic processes using a focussed radiant energy beam, such as a laser beam (11) to kill unwanted cells or to isolate wanted cells is described. The apparatus includes microscope means (14) and cell detection means (27) for identifying the cells to be killed or saved and attenuator means (12) for modifying the beam to prevent destruction of cells to be saved.
摘要翻译： 描述了基于使用聚焦辐射能束（例如激光束（11））去除不需要的细胞或隔离所需细胞的不同化学或物理特性或动态过程的活细胞位置选择的方法和装置。 该装置包括用于识别待杀死或保存的细胞的显微镜装置（14）和细胞检测装置（27）和用于修改光束的衰减装置（12），以防止要节省的细胞的破坏。
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公开(公告)号：US4629687A
公开(公告)日：1986-12-16
申请号：US403154
申请日：1982-07-29
IPC分类号： B07C5/342 , C12M1/34 , C12M3/00 , C12N1/00 , C12Q1/04 , G01N15/14 , G01N33/48 , C12N5/00 , C12Q1/00
CPC分类号： C12M47/06 , B07C5/342 , C12M47/04 , G01N2015/149
摘要： A method and apparatus for positive selection of viable cells based upon differing chemical or physical properties or dynamic processes using a focussed radiant energy beam, such as a laser beam (11) to kill unwanted cells is described. The apparatus includes a microscope (14) with a photomultiplier (27) which is used to selectively detect light from certain cells produced by an attenuated laser beam irradiating the cells for identifying the cells to be killed or saved. Based upon identification, selected cells to be killed are exposed to the unattenuated laser beam.
摘要翻译： 描述了基于不同的化学或物理性质或使用聚焦的辐射能量束（例如用于杀死不需要的细胞的激光束（11））的动态过程来正确选择活细胞的方法和装置。 该装置包括具有光电倍增器（27）的显微镜（14），该显微镜用于选择性地检测由照射细胞的衰减激光束产生的某些细胞的光，以识别待杀死或保存的细胞。 基于鉴定，待杀死的选定细胞暴露于未衰减的激光束。
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4.发明授权公开(公告)号：US08383408B2
公开(公告)日：2013-02-26
申请号：US12767578
申请日：2010-04-26
IPC分类号： C12N5/00
CPC分类号： B82Y5/00 , C08G63/06 , C12M25/14 , C12N5/0068 , C12N2533/40 , D01D5/0007 , D01F1/02 , D01F6/60 , D01F6/625 , D01F11/14
摘要： A nanofibrillar structure for cell culture and tissue engineering is disclosed. The nanofibrillar structure can be used in a variety of applications including methods for proliferating and/or differentiating cells and manufacturing a tissue. Also disclosed is an improved nanofiber comprising a lipid, lipophilic molecule, or chemically modified surface. The nanofibers can be used in a variety of applications including the formation of nanofibrillar structures for cell culture and tissue engineering.
摘要翻译： 公开了用于细胞培养和组织工程的纳米纤丝结构。 纳米纤丝结构可用于各种应用，包括增殖和/或分化细胞和制造组织的方法。 还公开了一种改进的纳米纤维，其包含脂质，亲脂性分子或化学改性的表面。 纳米纤维可用于各种应用，包括形成用于细胞培养和组织工程的纳米纤丝结构。
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5.发明授权公开(公告)号：US07704740B2
公开(公告)日：2010-04-27
申请号：US10703169
申请日：2003-11-05
IPC分类号： C12N5/00
CPC分类号： B82Y5/00 , C08G63/06 , C12M25/14 , C12N5/0068 , C12N2533/40 , D01D5/0007 , D01F1/02 , D01F6/60 , D01F6/625 , D01F11/14
摘要： A nanofibrillar structure for cell culture and tissue engineering is disclosed. The nanofibrillar structure can be used in a variety of applications including methods for proliferating and/or differentiating cells and manufacturing a tissue. Also disclosed is an improved nanofiber comprising a lipid, lipophilic molecule, or chemically modified surface. The nanofibers can be used in a variety of applications including the formation of nanofibrillar structures for cell culture and tissue engineering.
摘要翻译： 公开了用于细胞培养和组织工程的纳米纤丝结构。 纳米纤丝结构可用于各种应用，包括增殖和/或分化细胞和制造组织的方法。 还公开了一种改进的纳米纤维，其包含脂质，亲脂性分子或化学改性的表面。 纳米纤维可用于各种应用，包括形成用于细胞培养和组织工程的纳米纤丝结构。
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6.发明授权Method of converting cellulosic biomass to alcohol using web comprising fine fiber and bioactive particulate 有权使用包含细纤维和生物活性颗粒的纤维素将纤维素生物质转化为醇的方法公开(公告)号：US08334121B2
公开(公告)日：2012-12-18
申请号：US12279031
申请日：2007-02-13
CPC分类号： B01D39/1623 , B01D39/04 , B01D39/14 , B01D2239/0407 , B01D2239/0442 , B01D2239/0631 , B01D2239/064 , B01D2239/065 , B01D2239/1266 , C12M25/14 , C12N5/0068 , C12N2533/30 , C12N2533/78 , Y02E50/17 , Y10T428/249921 , Y10T428/29 , Y10T442/614
摘要： The assemblies of the invention can comprise a fine fiber layer forming a multilamellar web or matrix, having dispersed within the fine fiber layer a bioactive particulate material, including cells, enzymes or microorganisms. Fluid that flows through the assemblies of the invention can have any material dispersed or dissolved in the fluid react with, be absorbed by, or adsorbed onto, the bioactive particulate within the nanofiber layer. The assemblies of the invention can be used to treat or purify fluid streams. The assemblies of the invention can be used in conjunction with a bioreactor system, a bioartificial organ, or a culture container.
摘要翻译： 本发明的组件可以包含形成多层纤维网或基质的细纤维层，其在细纤维层内分散有生物活性颗粒材料，包括细胞，酶或微生物。 流过本发明的组件的流体可以具有分散或溶解在流体中的任何材料与纳米纤维层内的生物活性颗粒反应，吸收或吸附到其上。 本发明的组件可用于处理或净化流体流。 本发明的组件可以与生物反应器系统，生物人造器官或培养容器结合使用。
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7.发明授权DNA encoding carbohydrate binding protein and biological materials derived therefrom 失效编码碳水化合物结合蛋白的DNA和从其衍生的生物材料
公开(公告)号：US5863728A
公开(公告)日：1999-01-26
申请号：US675921
申请日：1996-07-05
IPC分类号： C07K14/195 , C12Q1/68
CPC分类号： C07K14/195
摘要： A DNA fragment encoding a carbohydrate binding lectin BJ38 in chromosomal DNA of Bradyrhizobium japonicum is described. The DNA is used as a source of the lectin, as a probe and as DNA for recombinant strains of rhizobium with enhanced nodulation and production in legumes.
摘要翻译： 描述了编码日本Bradyrhizobium染色体DNA中的碳水化合物结合凝集素BJ38的DNA片段。 该DNA用作凝集素的来源，作为探针，并用作具有增强的豆科植物结瘤和生产的根瘤菌重组菌株的DNA。
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8.发明申请公开(公告)号：US20100297768A1
公开(公告)日：2010-11-25
申请号：US12767578
申请日：2010-04-26
CPC分类号： B82Y5/00 , C08G63/06 , C12M25/14 , C12N5/0068 , C12N2533/40 , D01D5/0007 , D01F1/02 , D01F6/60 , D01F6/625 , D01F11/14
摘要： A nanofibrillar structure for cell culture and tissue engineering is disclosed. The nanofibrillar structure can be used in a variety of applications including methods for proliferating and/or differentiating cells and manufacturing a tissue. Also disclosed is an improved nanofiber comprising a lipid, lipophilic molecule, or chemically modified surface. The nanofibers can be used in a variety of applications including the formation of nanofibrillar structures for cell culture and tissue engineering.
摘要翻译： 公开了用于细胞培养和组织工程的纳米纤丝结构。 纳米纤丝结构可用于各种应用，包括增殖和/或分化细胞和制造组织的方法。 还公开了一种改进的纳米纤维，其包含脂质，亲脂性分子或化学改性的表面。 纳米纤维可用于各种应用，包括形成用于细胞培养和组织工程的纳米纤丝结构。
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公开(公告)号：US20090221047A1
公开(公告)日：2009-09-03
申请号：US12279031
申请日：2007-02-13
CPC分类号： B01D39/1623 , B01D39/04 , B01D39/14 , B01D2239/0407 , B01D2239/0442 , B01D2239/0631 , B01D2239/064 , B01D2239/065 , B01D2239/1266 , C12M25/14 , C12N5/0068 , C12N2533/30 , C12N2533/78 , Y02E50/17 , Y10T428/249921 , Y10T428/29 , Y10T442/614
摘要： The assemblies of the invention can comprise a fine fiber layer forming a multilamellar web or matrix, having dispersed within the fine fiber layer a bioactive particulate material, including cells, enzymes or microorganisms. Fluid that flows through the assemblies of the invention can have any material dispersed or dissolved in the fluid react with, be absorbed by, or adsorbed onto, the bioactive particulate within the nanofiber layer. The assemblies of the invention can be used to treat or purify fluid streams. The assemblies of the invention can be used in conjunction with a bioreactor system, a bioartificial organ, or a culture container.
摘要翻译： 本发明的组件可以包含形成多层纤维网或基质的细纤维层，其在细纤维层内分散有生物活性颗粒材料，包括细胞，酶或微生物。 流过本发明的组件的流体可以具有分散或溶解在流体中的任何材料与纳米纤维层内的生物活性颗粒反应，吸收或吸附到其上。 本发明的组件可用于处理或净化流体流。 本发明的组件可以与生物反应器系统，生物人造器官或培养容器结合使用。
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公开(公告)号：US06204067B1
公开(公告)日：2001-03-20
申请号：US09335457
申请日：1999-06-17
申请人： Sanford M. Simon , Melvin S. Schindler , Yu Chen
发明人： Sanford M. Simon , Melvin S. Schindler , Yu Chen
IPC分类号： G01N3122
CPC分类号： G01N21/6428 , G01N21/80 , G01N33/743 , G01N2021/6439 , G01N2333/723 , G01N2500/00 , Y10T436/173845
摘要： The present invention discloses the mechanism of tamoxifen mediated inhibition of acidification. This mechanism information is then used to design methods of identifying compounds that will have the pharmaceutical effect of tamoxifen without the corresponding side-effects.
摘要翻译： 本发明公开了他莫昔芬介导的酸化抑制机制。 然后将该机制信息用于设计鉴定化合物的方法，该化合物将具有没有相应副作用的他莫昔芬的药效。
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