公开(公告)号：US5804414A

公开(公告)日：1998-09-08

申请号：US674168

申请日：1996-07-01

申请人： Mika Moriya ,  Hiroshi Matsui ,  Kenzo Yokozeki ,  Seiko Hirano ,  Atsushi Hayakawa ,  Masako Izui ,  Masakazu Sugimoto

发明人： Mika Moriya ,  Hiroshi Matsui ,  Kenzo Yokozeki ,  Seiko Hirano ,  Atsushi Hayakawa ,  Masako Izui ,  Masakazu Sugimoto

IPC分类号： C12N1/21 ,  C12N9/02 ,  C12N9/12 ,  C12N9/88 ,  C12N15/67 ,  C12N15/77 ,  C12N15/87 ,  C12N15/90 ,  C12P13/04 ,  C12P13/08 ,  C12P21/00 ,  C12P21/04

CPC分类号： C12N9/001 ,  C12N15/67 ,  C12N15/77 ,  C12N15/90 ,  C12N9/1217 ,  C12N9/88 ,  C12P13/08

摘要： A method of amplifying a desired gene in a chromosome of a coryneform bacterium, which comprises forming an artificial transposon in which a drug resistance gene and the desired gene are inserted into an insertion sequence of the coryneform bacterium, and introducing said artificial transposon into the coryneform bacterium. In accordance with the method of the present invention, a desired gene can be amplified in a chromosome in coryneform bacteria which are used in the industrial production of amino acids or nucleic acids.

摘要翻译： 一种扩增棒状细菌染色体中所需基因的方法，其特征在于包括形成其中将耐药基因和所需基因插入棒状细菌的插入序列的人工转座子，并将所述人工转座子引入棒状细胞 细菌。 根据本发明的方法，可以在用于工业生产氨基酸或核酸的棒状细菌的染色体中扩增所需的基因。

公开(公告)号：US6090597A

公开(公告)日：2000-07-18

申请号：US852730

申请日：1997-05-07

申请人： Seiko Hirano ,  Masakazu Sugimoto ,  Eiichi Nakano ,  Masako Izui ,  Atsushi Hayakawa ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

发明人： Seiko Hirano ,  Masakazu Sugimoto ,  Eiichi Nakano ,  Masako Izui ,  Atsushi Hayakawa ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/21 ,  C12N9/06 ,  C12N9/88 ,  C12N15/77 ,  C12P13/08 ,  C12R1/13 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N9/0016 ,  C12N15/77 ,  C12N9/88 ,  C12P13/08

摘要： A coryneform bacterium in which a DNA coding for a diaminopimelate decarboxylase and a DNA coding for a diaminopimelate dehydrogenase are enhanced is cultivated in a medium to allow L-lysine to be produced and accumulated in a culture, and L-lysine is collected from the culture.

摘要翻译： 在培养基中培养其中编码二氨基庚二酸脱羧酶的DNA和编码二氨基庚二酸脱氢酶的DNA的棒状细菌在培养基中培养以使L-赖氨酸在培养物中产生并积累，从培养物中收集L-赖氨酸 。

公开(公告)号：US08183017B2

公开(公告)日：2012-05-22

申请号：US12915793

申请日：2010-10-29

申请人： Seiko Otsuna ,  Masakazu Sugimoto ,  Masako Izui ,  Atsushi Hayakawa ,  Eiichi Nakano ,  Masaki Kobayashi ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

发明人： Seiko Otsuna ,  Masakazu Sugimoto ,  Masako Izui ,  Atsushi Hayakawa ,  Eiichi Nakano ,  Masaki Kobayashi ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

IPC分类号： C12P13/04 ,  C12P21/04

CPC分类号： C12N9/0016 ,  C12N9/1217 ,  C12N9/88 ,  C12N15/52 ,  C12N15/77 ,  C12P13/08

摘要： The ability and speed with which a coryneform bacterium can produce L-lysine are improved when the coryneform bacterium contains an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized. This is accomplished by successively enhancing the DNA coding for dihydrodipicolinate reductase, the DNA coding for dihydrodipicolinate synthase, the DNA coding for diaminopimelate decarboxylase, and the DNA coding for diaminopimelate dehydrogenase.

摘要翻译： 当棒状细菌含有其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏的天冬氨酸激酶时​​，棒状细菌可以产生L-赖氨酸的能力和速度得到改善。 这是通过连续增强编码二氢吡啶二羧酸还原酶的DNA，编码二氢吡啶二羧酸合成酶的DNA，编码二氨基庚二酸脱羧酶的DNA和编码二氨基庚二酸脱氢酶的DNA来实现的。

公开(公告)号：US07846698B2

公开(公告)日：2010-12-07

申请号：US10226136

申请日：2002-08-23

申请人： Seiko Otsuna ,  Masakazu Sugimoto ,  Masako Izui ,  Atsushi Hayakawa ,  Eiichi Nakano ,  Masaki Kobayashi ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

发明人： Seiko Otsuna ,  Masakazu Sugimoto ,  Masako Izui ,  Atsushi Hayakawa ,  Eiichi Nakano ,  Masaki Kobayashi ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

IPC分类号： C12P13/04 ,  C12P21/04

CPC分类号： C12N9/0016 ,  C12N9/1217 ,  C12N9/88 ,  C12N15/52 ,  C12N15/77 ,  C12P13/08

摘要： The L-lysine-producing ability and the L-lysine-producing speed are improved in a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, by successively enhancing DNA coding for a dihydrodipicolinate reductase, DNA coding for a dihydrodipicolinate synthase, DNA coding for a diaminopimelate decarboxylase, and DNA coding for a diaminopimelate dehydrogenase.

摘要翻译： 通过连续增强编码二氢吡啶二羧酸还原酶的DNA，在含有赖氨酸和L-苏氨酸的反馈抑制实质上脱敏的天麸蛋白的棒状细菌的L-赖氨酸生产能力和L-赖氨酸生产速度得到改善， 编码二氢吡啶二羧酸合成酶的DNA，编码二氨基庚二酸脱羧酶的DNA，以及编码二氨基庚二酸脱氢酶的DNA。

公开(公告)号：US06893852B1

公开(公告)日：2005-05-17

申请号：US10019284

申请日：2000-06-30

申请人： Masako Izui ,  Masakazu Sugimoto ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

发明人： Masako Izui ,  Masakazu Sugimoto ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

IPC分类号： C12N9/12 ,  C12N15/54 ,  C07H21/04

CPC分类号： C12N9/1205 ,  C12Y207/01069

摘要： A gene encoding a protein constituting sucrose PTS of coryneform bacterium is provided by amplifying a region existing downstream from sucrase gene in coryneform bacterium by the cassette-ligation mediated PCR to obtain DNA encoding sucrose PTS enzyme II, which is a protein defined in the following (A) or (B): (A) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing; (B) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing including substitution, deletion, insertion, addition or inversion of one or several amino acids, and an activity for binding to sucrose.

摘要翻译： 通过盒式连接介导的PCR扩增棒状杆菌型细菌的蔗糖酶基因下游的区域，得到编码构成棒状细菌的蔗糖PTS的蛋白质的基因，得到编码下述定义的蛋白质的蔗糖PTS酶II的DNA（ A）或（B）：（A）序列表中具有SEQ ID NO：2的氨基酸序列的蛋白质; （B）序列表中具有SEQ ID NO：2的氨基酸序列的蛋白质，包括一个或几个氨基酸的取代，缺失，插入，添加或转化，以及与蔗糖结合的活性。

公开(公告)号：US06319696B1

公开(公告)日：2001-11-20

申请号：US09011762

申请日：1998-04-17

申请人： Hiroko Kishino ,  Masako Izui ,  Yukiko Ono ,  Hisao Ito ,  Osamu Kurahashi

发明人： Hiroko Kishino ,  Masako Izui ,  Yukiko Ono ,  Hisao Ito ,  Osamu Kurahashi

IPC分类号： C12P1308

CPC分类号： C12P13/04

摘要： A microorganism, which has an ability to produce L-amino acid, especially L-phenylalanine, L-tryptophane, L-tyrosine, L-threonine, or L-isoleucine, in which a phosphoenolpyruvate-producing ability is enhanced, is cultivated in a medium so that the L-amino acid is produced and accumulated in the medium to collect the L-amino acid.

摘要翻译： 具有生产L-氨基酸，特别是L-苯丙氨酸，L-色氨酸，L-酪氨酸，L-苏氨酸或L-异亮氨酸的能力的微生物，其中增加了产生磷酸烯醇丙酮酸的能力， 培养基中，使L-氨基酸产生并积累在培养基中以收集L-氨基酸。