公开(公告)号：US5804414A

公开(公告)日：1998-09-08

申请号：US674168

申请日：1996-07-01

申请人： Mika Moriya ,  Hiroshi Matsui ,  Kenzo Yokozeki ,  Seiko Hirano ,  Atsushi Hayakawa ,  Masako Izui ,  Masakazu Sugimoto

发明人： Mika Moriya ,  Hiroshi Matsui ,  Kenzo Yokozeki ,  Seiko Hirano ,  Atsushi Hayakawa ,  Masako Izui ,  Masakazu Sugimoto

IPC分类号： C12N1/21 ,  C12N9/02 ,  C12N9/12 ,  C12N9/88 ,  C12N15/67 ,  C12N15/77 ,  C12N15/87 ,  C12N15/90 ,  C12P13/04 ,  C12P13/08 ,  C12P21/00 ,  C12P21/04

CPC分类号： C12N9/001 ,  C12N15/67 ,  C12N15/77 ,  C12N15/90 ,  C12N9/1217 ,  C12N9/88 ,  C12P13/08

摘要： A method of amplifying a desired gene in a chromosome of a coryneform bacterium, which comprises forming an artificial transposon in which a drug resistance gene and the desired gene are inserted into an insertion sequence of the coryneform bacterium, and introducing said artificial transposon into the coryneform bacterium. In accordance with the method of the present invention, a desired gene can be amplified in a chromosome in coryneform bacteria which are used in the industrial production of amino acids or nucleic acids.

摘要翻译： 一种扩增棒状细菌染色体中所需基因的方法，其特征在于包括形成其中将耐药基因和所需基因插入棒状细菌的插入序列的人工转座子，并将所述人工转座子引入棒状细胞 细菌。 根据本发明的方法，可以在用于工业生产氨基酸或核酸的棒状细菌的染色体中扩增所需的基因。

公开(公告)号：US6090597A

公开(公告)日：2000-07-18

申请号：US852730

申请日：1997-05-07

申请人： Seiko Hirano ,  Masakazu Sugimoto ,  Eiichi Nakano ,  Masako Izui ,  Atsushi Hayakawa ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

发明人： Seiko Hirano ,  Masakazu Sugimoto ,  Eiichi Nakano ,  Masako Izui ,  Atsushi Hayakawa ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/21 ,  C12N9/06 ,  C12N9/88 ,  C12N15/77 ,  C12P13/08 ,  C12R1/13 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N9/0016 ,  C12N15/77 ,  C12N9/88 ,  C12P13/08

摘要： A coryneform bacterium in which a DNA coding for a diaminopimelate decarboxylase and a DNA coding for a diaminopimelate dehydrogenase are enhanced is cultivated in a medium to allow L-lysine to be produced and accumulated in a culture, and L-lysine is collected from the culture.

摘要翻译： 在培养基中培养其中编码二氨基庚二酸脱羧酶的DNA和编码二氨基庚二酸脱氢酶的DNA的棒状细菌在培养基中培养以使L-赖氨酸在培养物中产生并积累，从培养物中收集L-赖氨酸 。

公开(公告)号：US20090162907A1

公开(公告)日：2009-06-25

申请号：US12266921

申请日：2008-11-07

申请人： Hiroshi IZUI ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

发明人： Hiroshi IZUI ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

IPC分类号： C12P13/14

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01

摘要： A microorganism which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH; and a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

摘要翻译： 在含有饱和浓度的L-谷氨酸和碳源的液体培养基中，能够在特定pH下代谢碳源的微生物，并且具有以超过相应于饱和浓度的量累积L-谷氨酸的能力 在液体介质中的pH值; 以及通过发酵生产L-谷氨酸的方法，其包括在将pH调节至L-谷氨酸沉淀的pH的液体培养基中培养微生物以产生并积累L-谷氨酸并将L-谷氨酸沉淀， 培养基中的谷氨酸。

公开(公告)号：USRE42350E1

公开(公告)日：2011-05-10

申请号：US12429421

申请日：2009-04-24

申请人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

发明人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

IPC分类号： C12P13/14 ,  C12N15/00 ,  C12N1/20 ,  C12N1/36 ,  C12N15/63

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01

摘要： A microorganism is provided which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and which has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH. Also provided is a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

摘要翻译： 提供了能够在饱和浓度和碳源的含有L-谷氨酸的液体培养基中在特定pH下代谢碳源的微生物，并且具有将L-谷氨酸的积累量超过对应于 在液体介质中的饱和浓度在pH值。 还提供了通过发酵生产L-谷氨酸的方法，其包括在将pH调节至L-谷氨酸沉淀的pH的液体培养基中培养微生物以产生并积累L-谷氨酸并沉淀 培养基中的L-谷氨酸。

公开(公告)号：US07208296B2

公开(公告)日：2007-04-24

申请号：US11150265

申请日：2005-06-13

申请人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

发明人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

IPC分类号： C12P13/14 ,  C12N15/00 ,  C12N1/20 ,  C12N1/36 ,  C12N15/63

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01

摘要： A microorganism is provided which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and which has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH. Also provided is a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

摘要翻译： 提供了能够在饱和浓度和碳源的含有L-谷氨酸的液体培养基中在特定pH下代谢碳源的微生物，并且具有将L-谷氨酸的积累量超过对应于 在液体介质中的饱和浓度在pH值。 还提供了通过发酵生产L-谷氨酸的方法，其包括在将pH调节至L-谷氨酸沉淀的pH的液体培养基中培养微生物以产生并积累L-谷氨酸并沉淀 培养基中的L-谷氨酸。

公开(公告)号：US20070134773A1

公开(公告)日：2007-06-14

申请号：US11668079

申请日：2007-01-29

申请人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

发明人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

IPC分类号： C12P13/14 ,  C12N1/21

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01

摘要： A microorganism which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH; and a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

摘要翻译： 在含有饱和浓度的L-谷氨酸和碳源的液体培养基中，能够在特定pH下代谢碳源的微生物，并且具有以超过相应于饱和浓度的量累积L-谷氨酸的能力 在液体介质中的pH值; 以及通过发酵生产L-谷氨酸的方法，其包括在将pH调节至L-谷氨酸沉淀的pH的液体培养基中培养微生物，以产生并积累L-谷氨酸并将L-谷氨酸沉淀， 培养基中的谷氨酸。

公开(公告)号：US20050227334A1

公开(公告)日：2005-10-13

申请号：US11150265

申请日：2005-06-13

申请人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

发明人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

IPC分类号： C12N1/21 ,  C12N1/00 ,  C12N1/20 ,  C12N9/06 ,  C12N9/88 ,  C12N15/09 ,  C12N15/53 ,  C12N15/60 ,  C12P13/14 ,  C12R1/01

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01

摘要： A microorganism is provided which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and which has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH. Also provided is a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

摘要翻译： 提供了能够在饱和浓度和碳源的含有L-谷氨酸的液体培养基中在特定pH下代谢碳源的微生物，并且具有将L-谷氨酸的积累量超过对应于 在液体介质中的饱和浓度在pH值。 还提供了通过发酵生产L-谷氨酸的方法，其包括在将pH调节至L-谷氨酸沉淀的pH的液体培养基中培养微生物以产生并积累L-谷氨酸并沉淀 培养基中的L-谷氨酸。

公开(公告)号：US07015010B1

公开(公告)日：2006-03-21

申请号：US09641892

申请日：2000-08-18

申请人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

发明人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

IPC分类号： C12Q1/04

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01

摘要： A microorganism which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH; and a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

摘要翻译： 在含有饱和浓度的L-谷氨酸和碳源的液体培养基中，能够在特定pH下代谢碳源的微生物，并且具有以超过相应于饱和浓度的量累积L-谷氨酸的能力 在液体介质中的pH值; 以及通过发酵生产L-谷氨酸的方法，其包括在将pH调节至L-谷氨酸沉淀的pH的液体培养基中培养微生物以产生并积累L-谷氨酸并将L-谷氨酸沉淀， 培养基中的谷氨酸。

公开(公告)号：US08278074B2

公开(公告)日：2012-10-02

申请号：US13047260

申请日：2011-03-14

申请人： Jun Nakamura ,  Seiko Hirano ,  Hisao Ito

发明人： Jun Nakamura ,  Seiko Hirano ,  Hisao Ito

IPC分类号： C12P13/14 ,  C12N1/20

CPC分类号： C12P13/14 ,  C07K14/34

摘要： A coryneform bacterium that is modified by using a yggB gene so that L-glutamic acid-producing ability is enhanced as compared to a non-modified strains is cultured in a medium to cause accumulation of L-glutamic acid in the medium or bacterial cells, and L-glutamic acid is collected from the medium or cells.

摘要翻译： 通过在培养基中培养通过使用yggB基因修饰的棒状细菌，使得与非修饰菌株相比增强L-谷氨酸生产能力，以在培养基或细菌细胞中引起L-谷氨酸的积累， 从培养基或细胞收集L-谷氨酸。

公开(公告)号：US07867735B2

公开(公告)日：2011-01-11

申请号：US12034101

申请日：2008-02-20

申请人： Seiko Hirano ,  Jun Nakamura ,  Hisao Ito

发明人： Seiko Hirano ,  Jun Nakamura ,  Hisao Ito

IPC分类号： C12P21/00 ,  C12N1/20

CPC分类号： C12P13/14 ,  C07K14/34

摘要： A method for producing L-glutamic acid by culturing a coryneform bacterium which has L-glutamic acid producing ability and which has been modified so that expression of the fasR gene is enhanced in a medium to produce and accumulate L-glutamic acid in the medium or cells, and collecting L-glutamic acid from the medium or cells.

摘要翻译： 通过培养具有L-谷氨酸生产能力的棒状细菌生产L-谷氨酸的方法，其已被修饰以使培养基中的fasR基因的表达增强以产生和积累L-谷氨酸，或者 细胞，并从培养基或细胞中收集L-谷氨酸。