摘要:
A method of controlling an internal combustion engine when the engine is operating in an engine braking mode is provided. The engine operates in the engine braking mode and an exhaust valve for the cylinder is prematurely opened to dissipate power. The method includes controlling airflow to at least one cylinder based on a comparison of a desired mass airflow rate and an actual mass airflow rate such that the actual mass airflow rate tracks the desired mass airflow rate.
摘要:
New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.
摘要:
An SBS-formatted microfluidic device where the geometry of the plate defines an array of interrogation areas, and where each interrogation area encompasses at least one reaction site.
摘要:
An ultrasound system for inspecting an object is provided. The ultrasound system includes an electromagnetic acoustic transducer (EMAT) and a pulser-receiver system. The EMAT is configured to generate acoustic signals representative of the area being inspected and comprises a flux concentrating non-conductive pole piece separating a radio frequency (RF) coil and a magnet. The pulser-receiver system is coupled to the EMAT and configured to receive the acoustic signals and convert the acoustic signal to electrical signals.
摘要:
An ultrasound system for inspecting an object is provided. The ultrasound system includes an electromagnetic acoustic transducer (EMAT) and a pulser-receiver system. The EMAT is configured to generate acoustic signals representative of the area being inspected and comprises a flux concentrating non-conductive pole piece separating a radio frequency (RF) coil and a magnet. The pulser-receiver system is coupled to the EMAT and configured to receive the acoustic signals and convert the acoustic signal to electrical signals.
摘要:
An engine system includes a first fuel regulator adapted to control an amount of a first fuel supplied to the engine, a second fuel regulator adapted to control an amount of a second fuel supplied to the engine concurrently with the first fuel being supplied to the engine, and a controller coupled to at least the second fuel regulator. The controller is adapted to determine the amount of the second fuel supplied to the engine in a relationship to the amount of the first fuel supplied to the engine to operate in igniting the first fuel at a specified time in steady state engine operation and adapted to determine the amount of the second fuel supplied to the engine in a manner different from the relationship at steady state engine operation in transient engine operation.
摘要:
In some aspects, the present invention provides a method for estimating at least one measurement/object property of a metal object. The method includes generating a time-varying eddy current in a wall of the metal object utilizing a pulsed-signal transmitter. The method further includes measuring the time-varying eddy current, fitting the time-varying measured eddy current to a parameterized polynomial, and interpreting the parameterized polynomial to determine one or more measurement/object properties of the metal object.
摘要:
The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.
摘要:
An integrated fluidic circuit includes a substrate layer and a first structure coupled to the substrate layer and including a plurality of channels. The first structure is configured to provide for flow of one or more materials through the plurality of channels. The integrated fluidic circuit also includes a second structure coupled to the substrate layer. The second structure includes a plurality of control channels configured to receive an actuation pressure. The integrated fluidic circuit is characterized by a thickness of less than 1.5 mm.
摘要:
Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.