公开(公告)号：US20240246284A1

公开(公告)日：2024-07-25

申请号：US18243000

申请日：2023-09-06

Applicant: President and Fellows of Harvard College

Inventor： Jennifer A. Lewis ,  Brett G. Compton ,  Jordan R. Raney ,  Thomas J. Ober

IPC: B29C64/118 ,  B29C70/06 ,  B29K105/12 ,  B33Y10/00 ,  B33Y70/10 ,  B33Y80/00

CPC classification number: B29C64/118 ,  B29C70/06 ,  B33Y10/00 ,  B33Y70/10 ,  B33Y80/00 ,  B29K2105/124

Abstract: A filamentary structure extruded from a nozzle during 3D printing comprises a continuous filament including filler particles dispersed therein. At least some fraction of the filler particles in the continuous filament comprise high aspect ratio particles having a predetermined orientation with respect to a longitudinal axis of the continuous filament. The high aspect ratio particles may be at least partially aligned along the longitudinal axis of the continuous filament. In some embodiments, the high aspect ratio particles may be highly aligned along the longitudinal axis. Also or alternatively, at least some fraction of the high aspect ratio particles may have a helical orientation comprising a circumferential component and a longitudinal component, where the circumferential component is imparted by rotation of a deposition nozzle and the longitudinal component is imparted by translation of the deposition nozzle.

公开(公告)号：US11214768B2

公开(公告)日：2022-01-04

申请号：US15554963

申请日：2016-03-03

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Jennifer A. Lewis ,  Mark A. Skylar-Scott ,  David B. Kolesky ,  Kimberly A. Homan ,  Alex H. M. Ng ,  George M. Church

IPC: C12N5/00 ,  A61L27/38 ,  A61K35/545 ,  C12N5/071 ,  C12N5/0793 ,  C12N5/077 ,  C12N5/079 ,  B29C64/106 ,  B33Y10/00 ,  B33Y70/00 ,  B33Y80/00 ,  B29K105/00

Abstract: Methods of tissue engineering, and more particularly methods and compositions for generating various vascularized 3D tissues, such as 3D vascularized embryoid bodies and organoids are described. Certain embodiments relate to a method of generating functional human tissue, the method comprising embedding an embryoid body or organoid in a tissue construct comprising a first vascular network and a second vascular network, each vascular network comprising one or more interconnected vascular channels; exposing the embryoid body or organoid to one or more biological agents, a biological agent gradient, a pressure, and/or an oxygen tension gradient, thereby inducing angiogenesis of capillary vessels to and/or from the embryoid body or organoid; and vascularizing the embryoid body or organoid, the capillary vessels connecting the first vascular network to the second vascular network, thereby creating a single vascular network and a perfusable tissue structure.

公开(公告)号：US20210154998A1

公开(公告)日：2021-05-27

申请号：US17142575

申请日：2021-01-06

Applicant: President and Fellows of Harvard College

Inventor： Daniele Foresti ,  Jennifer A. Lewis ,  Armand Kurum

IPC: B41J2/14 ,  B33Y10/00 ,  B33Y30/00 ,  B29C64/112 ,  B33Y70/00 ,  A23P20/20 ,  B29C64/209 ,  A23G1/54 ,  A61L27/24 ,  A61L27/38 ,  A61L27/54 ,  B41M5/00 ,  C09D11/52

Abstract: A method of acoustophoretic printing comprises generating an acoustic field at a first end of an acoustic chamber fully or partially enclosed by sound-reflecting walls. The acoustic field interacts with the sound-reflecting walls and travels through the acoustic chamber. The acoustic field is enhanced in a chamber outlet at a second end of the acoustic chamber. An ink is delivered into a nozzle positioned within the acoustic chamber. The nozzle has a nozzle opening projecting into the chamber outlet. The ink travels through the nozzle and is exposed to the enhanced acoustic field at the nozzle opening, and a predetermined volume of the ink is ejected from the nozzle opening and out of the acoustic chamber.

公开(公告)号：US10702630B2

公开(公告)日：2020-07-07

申请号：US15567570

申请日：2016-05-04

Applicant: President and Fellows of Harvard College

Inventor： Jennifer A. Lewis ,  Kimberly A. Homan ,  David B. Kolesky ,  Ryan L. Truby ,  Mark A. Skylar-Scott

IPC: A61L27/38 ,  A61L27/36 ,  A61L27/50 ,  A61L27/54 ,  B33Y10/00 ,  B33Y80/00 ,  B29C64/106 ,  A61L27/18 ,  A61L27/56 ,  A61F2/04

Abstract: A 3D printed tubular construct, such as a nephron, with or without embedded vasculature as well as methods of printing tubular tissue constructs are described.

公开(公告)号：US10597545B2

公开(公告)日：2020-03-24

申请号：US15574716

申请日：2016-05-16

Applicant: President and Fellows of Harvard College

Inventor： Jennifer A. Lewis ,  Joseph T. Muth

IPC: C09D11/03 ,  C09D11/52 ,  B33Y70/00 ,  B29C64/106 ,  B28B1/00 ,  C04B38/10 ,  B29C64/245 ,  B29C64/209 ,  C08J9/00 ,  C08J9/12 ,  C04B103/48 ,  C04B111/00 ,  B29K105/04

Abstract: A foam ink composition for printing porous structures comprises stabilizing particles and gas bubbles dispersed in a solvent. The stabilizing particles comprise a predetermined interfacial energy so as to exhibit a contact angle with the solvent of from about 15° to about 90°. At least a portion of the stabilizing particles are positioned at interfacial regions between the solvent and the gas bubbles, thereby stabilizing the gas bubbles in the foam ink composition. A 3D printed hierarchical porous structure comprises one or more continuous filaments arranged in a predetermined pattern on a substrate, the one or more continuous filaments comprising a sintered material and including a porosity of at least about 40 vol. %.

公开(公告)号：US20200061910A1

公开(公告)日：2020-02-27

申请号：US16467412

申请日：2017-12-05

Applicant: President and Fellows of Harvard College ,  ETH Zurich (Swiss Federal Institute of Technology)

Inventor： Jennifer A. Lewis ,  Jochen Mueller ,  Jordan R. Raney ,  Kristina Shea

IPC: B29C64/209 ,  B29C64/118 ,  B33Y10/00 ,  B33Y30/00

Abstract: In one aspect, the present disclosure provides a nozzle for 3-D printing. The nozzle may include a first nozzle tip defining a first outlet, where the first nozzle tip includes a first channel extending therethrough. The nozzle may further include a second nozzle tip defining a second outlet, where the second nozzle tip includes a second channel extending therethrough, and where the first channel surrounds the second outlet. The second nozzle tip may be retracted longitudinally with respect to the first nozzle tip such that the second outlet of the second nozzle tip is located in the first channel.

公开(公告)号：US10464031B2

公开(公告)日：2019-11-05

申请号：US16126723

申请日：2018-09-10

Applicant: President and Fellows of Harvard College

Inventor： Jennifer A. Lewis ,  Thomas J. Ober

IPC: B41J2/21 ,  B41J2/175 ,  B01F15/00 ,  B01F13/00 ,  B01F7/06 ,  B01F3/12 ,  B01F3/08 ,  B28C5/02 ,  B05B1/30 ,  B01F7/24 ,  B28C5/12 ,  B33Y30/00 ,  B29C64/209

Abstract: A microfluidic printing nozzle for 3D printing may include a mixing chamber, a first inlet for connecting with a first ink source, the first inlet located at a first end of the mixing chamber, and a second inlet for connecting with a second ink source, the second inlet located at the first end of the mixing chamber. An outlet may be located at a second end of the mixing chamber, and a generally cylindrical impeller may be rotatably disposed in the mixing chamber between the first end and the second end. The cylindrical impeller may include an outer surface, and the outer surface of the impeller includes a groove, a protrusion, or both, to facilitate mixing of fluidic inks flowing from the first end to the second end of the mixing chamber.

公开(公告)号：US10117968B2

公开(公告)日：2018-11-06

申请号：US15146613

申请日：2014-11-04

Applicant: President and Fellows of Harvard College

Inventor： Jennifer A. Lewis ,  David B. Kolesky ,  Mark A. Skylar-Scott ,  Kimberly A. Homan ,  Ryan L. Truby ,  Amelia Sydney Gladman

IPC: A61L27/50 ,  B29C64/40 ,  B29C64/106 ,  A61L27/38 ,  B33Y10/00 ,  B33Y80/00 ,  A61F2/06 ,  A61L27/22 ,  A61F2/10 ,  A61L27/36 ,  C12M1/26

Abstract: A printed tissue construct comprises one or more tissue patterns, where each tissue pattern comprises a plurality of viable cells of one or more predetermined cell types. A network of vascular channels interpenetrates the one or more tissue patterns. An extracellular matrix composition at least partially surrounds the one or more tissue patterns and the network of vascular channels. A method of printing a tissue construct with embedded vasculature comprises depositing one or more cell-laden filaments, each comprising a plurality of viable cells, on a substrate to form one or more tissue patterns. Each of the one or more tissue patterns comprises one or more predetermined cell types. One or more sacrificial filaments, each comprising a fugitive ink, are deposited on the substrate to form a vascular pattern interpenetrating the one or more tissue patterns. The vascular pattern and the one or more tissue patterns are at least partially surrounded with an extracellular matrix composition. The fugitive ink is then removed to create vascular channels in the extracellular matrix composition, thereby forming an interpenetrating vascular network in a tissue construct.

公开(公告)号：US20180110901A1

公开(公告)日：2018-04-26

申请号：US15567570

申请日：2016-05-04

Applicant: President and Fellows of Harvard College

Inventor： Jennifer A. Lewis ,  Kimberly A. Homan ,  David B. Kolesky ,  Ryan L. Truby ,  Mark A. Skylar-Scott

IPC: A61L27/38 ,  A61L27/56 ,  A61L27/36 ,  A61L27/18 ,  B33Y10/00 ,  B33Y80/00 ,  B29C64/106

CPC classification number: A61L27/3804 ,  A61F2/04 ,  A61F2240/002 ,  A61L27/18 ,  A61L27/3633 ,  A61L27/3675 ,  A61L27/3808 ,  A61L27/3834 ,  A61L27/3878 ,  A61L27/3891 ,  A61L27/50 ,  A61L27/54 ,  A61L27/56 ,  A61L2300/62 ,  A61L2300/64 ,  A61L2400/08 ,  A61L2430/26 ,  A61L2430/32 ,  B29C64/106 ,  B33Y10/00 ,  B33Y80/00

Abstract: A 3D printed tubular construct, such as a nephron, with or without embedded vasculature as well as methods of printing tubular tissue constructs are described.

公开(公告)号：US11969935B2

公开(公告)日：2024-04-30

申请号：US16467394

申请日：2017-12-05

Applicant: President and Fellows of Harvard College

Inventor： Jennifer A. Lewis ,  Mark A. Skylar-Scott ,  Jochen Mueller

IPC: B29C64/106 ,  B29C48/02 ,  B29C48/05 ,  B29C48/255 ,  B29C48/285 ,  B29C48/30 ,  B29C48/92 ,  B29C64/209 ,  B29C64/321 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y40/00

CPC classification number: B29C64/106 ,  B29C48/02 ,  B29C48/05 ,  B29C48/255 ,  B29C48/2888 ,  B29C48/298 ,  B29C48/304 ,  B29C48/92 ,  B29C64/209 ,  B29C64/321 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y40/00

Abstract: In one aspect, the present disclosure provides a nozzle for a 3D printing system. The nozzle may include a flowpath with a material inlet and a material outlet. The nozzle may further include a valve in fluid communication with the flowpath between the material inlet and the material outlet, where the valve includes a closed state and an open state, where in the closed state the valve obstructs the flowpath between the material inlet and the material outlet, and where in the open state the material inlet is in fluid communication with the material outlet. The nozzle may further include a compensator in fluid communication with the flowpath, where the compensator includes a contracted state associated with the open state of the valve and an expanded state associated with the closed state of the valve.