摘要:
The invention relates to systems and methods for preparing and analyzing samples (e.g., mucosal samples) for a microorganism of interest. In particular, the systems and methods are useful for detecting one or more analytes characteristic of a microorganism (i.e., microbe) of interest, such as components of cell walls that are characteristic of a microbe, particularly Staphylococcus aureus.
摘要:
The invention relates to methods of analyzing a sample for a bacterium of interest. In particular, the methods involve an initial capture process that includes the use of one or more antibodies having antigenic specificities for one or more distinct analytes characteristic of the specific bacterium. After initial capture of a specific bacterium, techniques of analyzing involve colorimetric techniques, particularly using colorimetric sensors that include polydiacetylene (PDA) materials.
摘要:
In certain embodiments, the invention relates to methods of capturing bacterial whole cells that includes the use of two or more antibodies having antigenic specificities for two or more distinct analytes characteristic of the specific bacterium. In certain embodiments, the invention relates to methods of analyzing a sample for a bacterium of interest. In particular, the methods are useful for detecting one or more analytes characteristic of a bacterium of interest, such as components of cell walls that are characteristic of a bacterium, particularly Staphylococcus aureus.
摘要:
A method of filtering a liquid sample that includes passing a sample comprising at least one biological organism through a filter membrane at a passive water volume flux of at least 10 L/m2·h·psi, wherein the filter membrane comprises a Bubble Point pore size of no more than 1.0 μm, thereby retaining at least one biological organism on the surface of the membrane; and detecting the at least one biological organism retained on the surface of the filter membrane.
摘要:
Methods for preparing high avidity anti-antigen polyclonal antibody preparations by antigen affinity column purification and high avidity anti-antigen polyclonal antibody preparations are described.
摘要:
An optical sensing system and method is disclosed. In one embodiment, a method of detecting a scattering center includes the step of providing an optical sensing system including a light source, one or more bus waveguides where a first bus waveguide has an input port that is in optical communication with the light source, a microresonator optically coupled to the one or more bus waveguides, and a scattering center which is capable of optically coupling to the microresonator. The method further includes the steps of exciting at least a first resonant guided optical mode of the microresonator with the light source, altering a strength of optical coupling between the scattering center and the microresonator to induce a change in optical scattering between the first mode and at least a second guided optical mode of the microresonator, and detecting a change in transfer of energy from the first mode to the second mode.
摘要:
A microresonator sensor apparatus has a microcavity resonator that defines equatorial whispering gallery modes (EWGMs), whose frequencies are separated by the free spectral range (FSR). The EWGMs lie in a plane perpendicular to a microcavity resonator axis. A light source is optically coupled to inject light into the microcavity resonator. The light source produces output light having an output spectrum whose bandwidth is approximately equal to or broader than the FSR of the EGWMs. One or more fluorescent materials are excited using the excitation light coupled into the microcavity resonator. A fluorescent signal arising from fluorescence of the one or more fluorescent materials is then detected.
摘要:
A biosensor system is provided for detecting biological species such as bacteria, proteins, viruses, spores and DNA or RNA. The biosensor system may also be able to distinguish between Gram positive and Gram negative bacteria. In some embodiments, the analyte solution flows past a detector surface to a filter, which traps the analyte. The analyte is then washed back past the detector surface, thus increasing the number of analyte species that become attached to the surface. The detector may include an optical microresonator that is optically coupled via waveguides to a light source and an optical detector. One of the waveguides may be provided with a wavelength selective reflector to increase the amount of probe light coupled into the microresonator or to increase the fraction of the signal light detected by the optical detector.
摘要:
An optical device and a sensor system incorporating same are disclosed. The optical device includes a microresonator that has a core with input and output ports. The output port is different than the input port. The optical device further includes first and second optical waveguides. Each optical waveguide has a core with input and output faces. The output face of the core of the first optical waveguide physically contacts the input port of the core of the microresonator. The input face of the core of the second optical waveguide physically contacts the output port of the core of the microresonator.