公开(公告)号：US20080286161A1

公开(公告)日：2008-11-20

申请号：US12109653

申请日：2008-04-25

申请人： Paul Heaney ,  Chao Lin ,  David Opalsky ,  Phillip Bruce, III ,  Charles Griswold ,  Sheila A. Walker ,  Ralf Worl ,  Andrzej Maczuszenko

发明人： Paul Heaney ,  Chao Lin ,  David Opalsky ,  Phillip Bruce, III ,  Charles Griswold ,  Sheila A. Walker ,  Ralf Worl ,  Andrzej Maczuszenko

IPC分类号： B01L3/00

CPC分类号： B01L9/527 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L7/52 ,  B01L9/523 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0877 ,  B01L2300/1827 ,  B01L2300/1844 ,  C12Q1/6827

摘要： Apparatus and methods for carrying out biochemical processes directly on a substrate are provided. A substrate assembly includes a cartridge that removably supports a substrate in a fixed position, and a reaction containment member that is removably located on top of the substrate. The reaction containment member includes one or more cavities that form chambers directly above one or more target locations on the surface of the substrate. The chambers can be used to conduct biochemical processes directly over the substrate, as well as to perform thermal cycling of material contained inside the chamber using a heating element disposed directly on the substrate. The substrate assembly is preferably used in combination with a processing machine that dispenses materials into the chambers and that conducts biochemical reactions of materials contained within the chambers, without requiring the substrate assembly to be moved from one location to another location during the processes.

摘要翻译： 提供了用于在基板上直接进行生物化学处理的装置和方法。 基板组件包括可拆卸地将基板支撑在固定位置的盒，以及可移除地位于基板顶部上的反应容纳构件。 反应容纳构件包括一个或多个空腔，其在衬底表面上的一个或多个目标位置上直接形成腔室。 这些腔室可以用于直接在衬底上进行生物化学过程，并且使用直接设置在衬底上的加热元件来对包含在腔室内的材料进行热循环。 衬底组件优选地与加工机器组合使用，该加工机器将材料分配到室中并且在室内进行包含的材料的生化反应，而不需要在工艺期间将衬底组件从一个位置移动到另一个位置。

公开(公告)号：US08088573B2

公开(公告)日：2012-01-03

申请号：US12109653

申请日：2008-04-25

申请人： Paul Heaney ,  Chao Lin ,  David Opalsky ,  Phillip Bruce, III ,  Charles Griswold ,  Sheila A. Walker ,  Ralf Worl ,  Andrzej Maczuszenko

发明人： Paul Heaney ,  Chao Lin ,  David Opalsky ,  Phillip Bruce, III ,  Charles Griswold ,  Sheila A. Walker ,  Ralf Worl ,  Andrzej Maczuszenko

IPC分类号： C12Q1/68 ,  C12M3/00 ,  C12M1/36

CPC分类号： B01L9/527 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L7/52 ,  B01L9/523 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0877 ,  B01L2300/1827 ,  B01L2300/1844 ,  C12Q1/6827

摘要： Apparatus and methods for carrying out biochemical processes directly on a substrate are provided. A substrate assembly includes a cartridge that removably supports a substrate in a fixed position, and a reaction containment member that is removably located on top of the substrate. The reaction containment member includes one or more cavities that form chambers directly above one or more target locations on the surface of the substrate. The chambers can be used to conduct biochemical processes directly over the substrate, as well as to perform thermal cycling of material contained inside the chamber using a heating element disposed directly on the substrate. The substrate assembly is preferably used in combination with a processing machine that dispenses materials into the chambers and that conducts biochemical reactions of materials contained within the chambers, without requiring the substrate assembly to be moved from one location to another location during the processes.

摘要翻译： 提供了用于在基板上直接进行生物化学处理的装置和方法。 基板组件包括可拆卸地将基板支撑在固定位置的盒，以及可移除地位于基板顶部上的反应容纳构件。 反应容纳构件包括一个或多个空腔，其在衬底表面上的一个或多个目标位置上直接形成腔室。 这些腔室可以用于直接在衬底上进行生物化学过程，并且使用直接设置在衬底上的加热元件来对包含在腔室内的材料进行热循环。 衬底组件优选地与加工机器组合使用，该加工机器将材料分配到室中并且在室内进行包含的材料的生化反应，而不需要在工艺期间将衬底组件从一个位置移动到另一位置。

公开(公告)号：US07364897B2

公开(公告)日：2008-04-29

申请号：US10412801

申请日：2003-04-11

申请人： Paul Heaney ,  Chao Lin ,  David Opalsky ,  Phillip Bruce, III ,  Charles Griswold ,  Sheila A. Walker ,  Ralf Wörl ,  Andrzej Maczuszenko

发明人： Paul Heaney ,  Chao Lin ,  David Opalsky ,  Phillip Bruce, III ,  Charles Griswold ,  Sheila A. Walker ,  Ralf Wörl ,  Andrzej Maczuszenko

IPC分类号： C12M1/34 ,  C12M3/00 ,  C12M1/22

CPC分类号： B01L9/527 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L7/52 ,  B01L9/523 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0877 ,  B01L2300/1827 ,  B01L2300/1844 ,  C12Q1/6827

摘要： Apparatus and methods for carrying out biochemical processes directly on a substrate are provided. A substrate assembly includes a cartridge that removably supports a substrate in a fixed position, and a reaction containment member that is removably located on top of the substrate. The reaction containment member includes one or more cavities that form chambers directly above one or more target locations on the surface of the substrate. The chambers can be used to conduct biochemical processes directly over the substrate, as well as to perform thermal cycling of material contained inside the chamber using a heating element disposed directly on the substrate. The substrate assembly is preferably used in combination with a processing machine that dispenses materials into the chambers and that conducts biochemical reactions of materials contained within the chambers, without requiring the substrate assembly to be moved from one location to another location during the processes.

摘要翻译： 提供了用于在基板上直接进行生物化学处理的装置和方法。 基板组件包括可拆卸地将基板支撑在固定位置的盒，以及可移除地位于基板顶部上的反应容纳构件。 反应容纳构件包括一个或多个空腔，其在衬底表面上的一个或多个目标位置上直接形成腔室。 这些腔室可以用于直接在衬底上进行生物化学过程，并且使用直接设置在衬底上的加热元件来对包含在腔室内的材料进行热循环。 衬底组件优选地与加工机器组合使用，该加工机器将材料分配到室中并且在室内进行包含的材料的生化反应，而不需要在工艺期间将衬底组件从一个位置移动到另一位置。

公开(公告)号：US20100240136A1

公开(公告)日：2010-09-23

申请号：US12785257

申请日：2010-05-21

申请人： Cindra A. Widrig Opalsky ,  David Opalsky ,  Andrzej MacZuszenko ,  Imants R. Lauks ,  Rhonda J. Cheadle

发明人： Cindra A. Widrig Opalsky ,  David Opalsky ,  Andrzej MacZuszenko ,  Imants R. Lauks ,  Rhonda J. Cheadle

IPC分类号： G01N33/86

CPC分类号： C12Q1/56 ,  G01N33/86 ,  Y10T436/11 ,  Y10T436/2575

摘要： This invention is a disposable cartridge for use at the patient side to perform traditional coagulation assays on fresh whole blood or blood derivative samples. The cartridge, in use with an electronic analyzer allows a fluid sample to be metered and quantitatively mixed with reagents which activate the coagulation cascade. An artificial substrate for thrombin, the enzyme whose action results in clot formation is also provided. Clot formation is subsequently detected using a microfabricated sensor also housed within the cartridge which detects electrochemically the product of the thrombin reaction upon the synthetic substrate.

公开(公告)号：US07977106B2

公开(公告)日：2011-07-12

申请号：US12785191

申请日：2010-05-21

申请人： Cindra A. Widrig Opalsky ,  David Opalsky ,  Andrzej Maczuszenko ,  Imants R. Lauks ,  Rhonda J. Cheadle

发明人： Cindra A. Widrig Opalsky ,  David Opalsky ,  Andrzej Maczuszenko ,  Imants R. Lauks ,  Rhonda J. Cheadle

IPC分类号： G01N33/86

CPC分类号： C12Q1/56 ,  G01N33/86 ,  Y10T436/11 ,  Y10T436/2575

摘要： This invention is a disposable cartridge for use at the patient side to perform traditional coagulation assays on fresh whole blood or blood derivative samples. The cartridge, in use with an electronic analyzer allows a fluid sample to be metered and quantitatively mixed with reagents which activate the coagulation cascade. An artificial substrate for thrombin, the enzyme whose action results in clot formation is also provided. Clot formation is subsequently detected using a microfabricated sensor also housed within the cartridge which detects electrochemically the product of the thrombin reaction upon the synthetic substrate.

公开(公告)号：US07923256B2

公开(公告)日：2011-04-12

申请号：US12785257

申请日：2010-05-21

申请人： Cindra A. Widrig Opalsky ,  David Opalsky ,  Andrzej MacZuszenko ,  Imants R. Lauks ,  Rhonda J. Cheadle

发明人： Cindra A. Widrig Opalsky ,  David Opalsky ,  Andrzej MacZuszenko ,  Imants R. Lauks ,  Rhonda J. Cheadle

IPC分类号： G01N33/86

CPC分类号： C12Q1/56 ,  G01N33/86 ,  Y10T436/11 ,  Y10T436/2575

摘要： This invention is a disposable cartridge for use at the patient side to perform traditional coagulation assays on fresh whole blood or blood derivative samples. The cartridge, in use with an electronic analyzer allows a fluid sample to be metered and quantitatively mixed with reagents which activate the coagulation cascade. An artificial substrate for thrombin, the enzyme whose action results in clot formation is also provided. Clot formation is subsequently detected using a microfabricated sensor also housed within the cartridge which detects electrochemically the product of the thrombin reaction upon the synthetic substrate.

摘要翻译： 本发明是用于患者侧的一次性药筒，用于对新鲜全血或血液衍生物样品进行传统的凝血测定。 与电子分析仪一起使用的墨盒允许将流体样品计量并与激活凝血级联的试剂定量混合。 还提供了用于凝血酶的人造底物，其作用导致凝块形成。 随后使用也容纳在药筒内的微制造传感器检测凝块形成，其中电化学地检测合成基质上凝血酶反应的产物。

公开(公告)号：US20100240067A1

公开(公告)日：2010-09-23

申请号：US12785191

申请日：2010-05-21

申请人： Cindra A. Widrig Opalsky ,  David Opalsky ,  Andrzej MacZuszenko ,  Imants R. Lauks ,  Rhonda J. Cheadle

发明人： Cindra A. Widrig Opalsky ,  David Opalsky ,  Andrzej MacZuszenko ,  Imants R. Lauks ,  Rhonda J. Cheadle

IPC分类号： G01N33/573

CPC分类号： C12Q1/56 ,  G01N33/86 ,  Y10T436/11 ,  Y10T436/2575

摘要： This invention is a disposable cartridge for use at the patient side to perform traditional coagulation assays on fresh whole blood or blood derivative samples. The cartridge, in use with an electronic analyzer allows a fluid sample to be metered and quantitatively mixed with reagents which activate the coagulation cascade. An artificial substrate for thrombin, the enzyme whose action results in clot formation is also provided. Clot formation is subsequently detected using a microfabricated sensor also housed within the cartridge which detects electrochemically the product of the thrombin reaction upon the synthetic substrate.

摘要翻译： 本发明是用于患者侧的一次性药筒，用于对新鲜全血或血液衍生物样品进行传统的凝血测定。 与电子分析仪一起使用的墨盒允许将流体样品计量并与激活凝血级联的试剂定量混合。 还提供了用于凝血酶的人造底物，其作用导致凝块形成。 随后使用也容纳在药筒内的微制造传感器检测凝块形成，其中电化学地检测合成基质上凝血酶反应的产物。

公开(公告)号：US08662392B2

公开(公告)日：2014-03-04

申请号：US13293529

申请日：2011-11-10

申请人： Norbert D. Hagen ,  David Opalsky

发明人： Norbert D. Hagen ,  David Opalsky

IPC分类号： G06F17/00

CPC分类号： G06Q10/087 ,  G01N35/00732 ,  G01N2035/00752 ,  G01N2035/00782 ,  G01N2035/00801 ,  G01N2035/00831 ,  G01N2035/00841

摘要： A system and method for tracking items during a process employs a rack configured to hold one or more receptacles. Each receptacle includes a machine-readable label providing information regarding the receptacle, and each receptacle-receiving position of the rack has associated therewith a machine-readable label identifying the receptacle receiving location. The rack also includes a memory element to which electronic data may be written. A label reading device reads the machine-readable label on each receptacle and the position-indicating, machine-readable labels on the rack. Information obtained from the machine-readable labels is written to the memory element, so that for subsequent processing of the receptacles carried on the rack, information relating to each of the receptacles can be obtained by reading the information written to the memory element. A tamper prevention element provides an indication of whether the rack may have been tampered with after data is written to the memory element.

摘要翻译： 用于在过程中跟踪物品的系统和方法采用配置成容纳一个或多个容器的架子。 每个插座包括提供关于插座的信息的机器可读标签，并且机架的每个插座接收位置具有标识容器接收位置的机器可读标签。 机架还包括可以写入电子数据的存储元件。 标签读取装置读取每个插座上的机器可读标签和机架上的位置指示机器可读标签。 从机器可读标签获得的信息被写入存储元件，因此为了随后处理在机架上承载的插座，可以通过读取写入存储元件的信息来获得与每个插座有关的信息。 防篡改元件提供了在将数据写入存储器元件之后机架是否可能被篡改的指示。

公开(公告)号：US09011771B2

公开(公告)日：2015-04-21

申请号：US12781390

申请日：2010-05-17

申请人： Norbert D. Hagen ,  Byron J. Knight ,  David Opalsky

发明人： Norbert D. Hagen ,  Byron J. Knight ,  David Opalsky

IPC分类号： G01N35/04 ,  G01N35/10 ,  B03C1/28 ,  G01N35/00 ,  B03C1/033

CPC分类号： B01L3/52 ,  B01L3/561 ,  B01L9/52 ,  B01L2300/0609 ,  B01L2300/12 ,  B03C1/0332 ,  B03C1/12 ,  B03C1/14 ,  B03C1/28 ,  B03C1/286 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  B25J11/00 ,  B65B3/04 ,  C12N15/1013 ,  G01N35/0098 ,  Y10T29/49815

摘要： Methods, systems, and apparatus are provided for automated isolation of selected analytes, to which magnetically-responsive solid supports are bound, from other components of a sample. An apparatus for performing an automated magnetic separation procedure includes a mechanism for effecting linear movement of a magnet between operative and non-operative positions with respect to a receptacle device. A receptacle holding station within which a receptacle device may be temporarily stored prior to moving the receptacle to the apparatus for performing magnetic separation includes magnets for applying a magnetic field to the receptacle device held therein, thereby drawing at least a proton of the magnetically-responsive solid supports out of suspension before the receptacle device is moved to the magnetic separation station. An automated receptacle transport mechanism moves the receptacle devices between the apparatus for performing magnetic separation and the receptacle holding station.

摘要翻译： 提供了方法，系统和装置，用于自动分离来自样品的其它组分的选定的分析物，其中磁性响应性固体支持物被结合。 用于执行自动磁分离程序的装置包括用于在相对于插座装置的操作位置和非操作位置之间实现磁体的线性移动的机构。 在将容器移动到用于执行磁性分离的装置之前可以临时存储插座装置的容器保持站包括用于向保持在其中的插座装置施加磁场的磁体，从而至少吸取磁响应的质子 在插座装置移动到磁选分离台之前，固体支撑体不能悬挂。 自动插座传送机构使插座装置在用于执行磁分离的装置和插座保持台之间移动。

公开(公告)号：US08731712B2

公开(公告)日：2014-05-20

申请号：US13320746

申请日：2010-05-17

申请人： Norbert D. Hagen ,  Byron J. Knight ,  David Opalsky ,  Jason Rhubottom ,  Olaf Hörger ,  Harald Thahedl ,  Heiko Sayer ,  Stefan Habrich

发明人： Norbert D. Hagen ,  Byron J. Knight ,  David Opalsky ,  Jason Rhubottom ,  Olaf Hörger ,  Harald Thahedl ,  Heiko Sayer ,  Stefan Habrich

IPC分类号： G06F7/00

CPC分类号： B65G47/901 ,  G01N35/026 ,  G01N35/04 ,  G01N2035/0413 ,  G01N2035/0422 ,  G01N2035/0484

摘要： Systems for effecting transfer of reaction receptacles between modules of an instrument for performing multi-step analytical procedures and for adjusting for variations in the positions of such modules includes a receptacle carrier supported on a track on which the carrier translates between different modules located adjacent to the track. The carrier includes a receptacle distribution head supported on a receptacle carrier carriage. The distribution head includes a receptacle moving mechanism adapted to move receptacles into and out of the distribution head and into and out of a module. The carriage is engaged with the track and adapted for translation along the track. Drive systems effect powered translation of the carriage, powered elevation and rotation of the distribution head, and powered movement of receptacles into and out of the distribution head. A transfer position locating system automatically determines a receptacle transfer position of the receptacle carrier with respect to each module.

摘要翻译： 用于在仪器的模块之间进行反应容器的传送以用于执行多步骤分析程序并用于调整这些模块的位置变化的系统包括支撑在轨道上的插座载体，载体在载体上在不同模块之间平移， 跟踪。 托架包括支撑在插座托架上的插座分配头。 分配头包括容纳移动机构，其适于将容器移入和移出分配头并进出模块。 滑架与轨道接合并适应轨道的翻译。 驱动系统影响滑架的动力翻转，分配头的动力升降和旋转，以及插座进出分配头的动力运动。 传送位置定位系统自动确定插座托架相对于每个模块的插座传送位置。