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    Transcriptional coactivator that interacts with Tat protein and regulates its binding to TAR RNA, methods for modulating Tat transactivation, and uses therefor
    7.
    发明授权
    Transcriptional coactivator that interacts with Tat protein and regulates its binding to TAR RNA, methods for modulating Tat transactivation, and uses therefor 有权
    与Tat蛋白相互作用并调节其与TAR RNA的结合的转录共激活因子，用于调节Tat反式激活的方法及其用途

    公开(公告)号：US06284456B1

    公开(公告)日：2001-09-04

    申请号：US09476482

    申请日：1999-12-30

    申请人： Katherine A. Jones ,  Ping Wei ,  Mitchell Garber ,  Shi-Min Fang

    发明人： Katherine A. Jones ,  Ping Wei ,  Mitchell Garber ,  Shi-Min Fang

    IPC分类号： C12Q170

    CPC分类号： C12N15/85 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/47 ,  C07K2319/00

    摘要： In accordance with the present invention, isolated nucleic acid encoding a host cell protein that regulates Tat transactivation has been discovered. The protein is the first discovered constituent of the TAK/TEFb complex which associates with the HIV Tat, via divalent cation metals, and is necessary for the binding of Tat to TAR RNA. This protein, cyclin T1, is an 87 kDa cyclin partner for the PITALRE kinase. It is further discovered that Tat must interact with TAK in order to bind to TAR RNA with affinity and with the appropriate sequence specificity that is observed in vivo. In accordance with another aspect of the invention, formulations useful for modulation of Tat transactivation have been developed. In addition, assays have been developed for the identification of compounds useful to modulate the above-described processes.

    摘要翻译： 根据本发明，已经发现了编码调节Tat反式激活的宿主细胞蛋白质的分离的核酸。 该蛋白质是TAK / TEFb复合物中首次发现的成分，其通过二价阳离子金属与HIV Tat结合，并且是Tat与TAR RNA结合所必需的。 这种蛋白质，细胞周期蛋白T1，是PITALRE激酶的87kDa细胞周期蛋白配偶体。 进一步发现，Tat必须与TAK相互作用以便以亲和力和在体内观察到的适当的序列特异性结合TAR RNA。 根据本发明的另一方面，已经开发了可用于调节Tat反式激活的制剂。 此外，已经开发了用于鉴定可用于调节上述过程的化合物的测定。