公开(公告)号：US11718823B2

公开(公告)日：2023-08-08

申请号：US17108009

申请日：2020-12-01

Applicant: Purdue Research Foundation

Inventor： Bumsoo Han ,  George Tsu-Chih Chiu ,  Cih Cheng

IPC: B33Y10/00 ,  B33Y70/00 ,  C12N5/00 ,  C12N5/0775 ,  C12N5/071 ,  C12N5/09

CPC classification number: C12N5/0062 ,  B33Y10/00 ,  B33Y70/00 ,  C12N5/0663 ,  C12N5/0693 ,  C12N5/0697 ,  C12N2513/00

Abstract: The present disclosure relates to a novel method for rapid preparation of three dimensional (3D) spheroids/organoids, and the 3D spheroids/organoids prepared by the novel method.

公开(公告)号：US11273447B2

公开(公告)日：2022-03-15

申请号：US16741222

申请日：2020-01-13

Applicant: Purdue Research Foundation

Inventor： Bumsoo Han ,  George Tsu-Chih Chiu ,  Thomas Heinrich Siegmund ,  Michael Linnes ,  Yumeng Wu ,  Matthew Tyler Short

IPC: C12M3/00 ,  C12M1/00 ,  B01L3/00

Abstract: Multi-well collapsible basket arrays and methods for their use with high throughput culture and histology analysis of spheroids and organoids. Such a collapsible basket array includes a collapsible cellular array structure having multiple cells and connectors that interconnect adjacent pairs of the cells to cause the collapsible cellular array structure to collapse from an expanded configuration to a collapsed configuration in which the connectors are partially wrapped around perimeters or circumferences of the cells, whereby the collapsible cellular array structure is expandable to acquire an expanded configuration capable of individually aligning the cells thereof with wells of a well plate. The collapsible basket array further includes inserts individually mountable to the cells of the collapsible cellular array structure, with each insert including a permeable basket with pores sized to retain spheroids or organoids within the basket.

公开(公告)号：US20200222898A1

公开(公告)日：2020-07-16

申请号：US16741222

申请日：2020-01-13

Applicant: Purdue Research Foundation

Inventor： Bumsoo Han ,  George Tsu-Chih Chiu ,  Thomas Heinrich Siegmund ,  Michael Linnes ,  Yument Wu ,  Matthew Tyler Short

IPC: B01L3/00 ,  C12M1/00 ,  C12M3/00

Abstract: Multi-well collapsible basket arrays and methods for their use with high throughput culture and histology analysis of spheroids and organoids. Such a collapsible basket array includes a collapsible cellular array structure having multiple cells and connectors that interconnect adjacent pairs of the cells to cause the collapsible cellular array structure to collapse from an expanded configuration to a collapsed configuration in which the connectors are partially wrapped around perimeters or circumferences of the cells, whereby the collapsible cellular array structure is expandable to acquire an expanded configuration capable of individually aligning the cells thereof with wells of a well plate. The collapsible basket array further includes inserts individually mountable to the cells of the collapsible cellular array structure, with each insert including a permeable basket with pores sized to retain spheroids or organoids within the basket.

公开(公告)号：US12016690B2

公开(公告)日：2024-06-25

申请号：US16779695

申请日：2020-02-03

Applicant: Purdue Research Foundation

Inventor： Bumsoo Han ,  Yingnan Shen

IPC: G06T7/00 ,  A61B5/00 ,  A61B5/15 ,  A61L27/38 ,  C12M1/26 ,  C12M1/34 ,  G01N21/00 ,  G01N21/64 ,  G01N33/483 ,  G09B23/30

CPC classification number: A61B5/150106 ,  A61B5/0033 ,  A61B5/0064 ,  A61B5/0077 ,  A61B5/150167 ,  A61L27/3804 ,  C12M33/04 ,  C12M41/46 ,  G01N21/00 ,  G01N21/6456 ,  G01N33/4833 ,  G06T7/0012 ,  G09B23/306 ,  G06T2207/10064 ,  G06T2207/30088

Abstract: Disclosed herein is a platform and method to quantify spatiotemporal tissue swelling during biologics injection, and to predict associated increase in the mechanical stress and interstitial fluid pressure (IFP) of tissues. Accurate measure and estimation of tissue swelling, thus, can be quantitative and predictive indicator of the IPD.

公开(公告)号：US20210238541A1

公开(公告)日：2021-08-05

申请号：US17108009

申请日：2020-12-01

Applicant: Purdue Research Foundation

Inventor： Bumsoo Han ,  George Tsu-Chih Chiu ,  Cih Cheng

IPC: C12N5/00 ,  C12N5/0775 ,  C12N5/071 ,  C12N5/09 ,  B33Y70/00 ,  B33Y10/00

Abstract: The present disclosure relates to a novel method for rapid preparation of three dimensional (3D) spheroids/organoids, and the 3D spheroids/organoids prepared by the novel method.

公开(公告)号：US20200253522A1

公开(公告)日：2020-08-13

申请号：US16779695

申请日：2020-02-03

Applicant: Purdue Research Foundation

Inventor： Bumsoo Han ,  Yingnan Shen

IPC: A61B5/15 ,  A61B5/00 ,  A61L27/38 ,  G06T7/00

Abstract: Disclosed herein is a platform and method to quantify spatiotemporal tissue swelling during biologics injection, and to predict associated increase in the mechanical stress and interstitial fluid pressure (IFP) of tissues. Accurate measure and estimation of tissue swelling, thus, can be quantitative and predictive indicator of the IPD.

公开(公告)号：US09081003B2

公开(公告)日：2015-07-14

申请号：US13886810

申请日：2013-05-03

Applicant: Purdue Research Foundation

Inventor： Kinam Park ,  Bumsoo Han ,  Bongseop Kwak ,  Crystal Soo Jung Shin

IPC: G01N33/50 ,  C12M3/00 ,  C12M1/12 ,  B01L3/00

CPC classification number: G01N33/5011 ,  B01L3/5027 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/163 ,  C12M21/08 ,  C12M25/04 ,  G01N33/5026 ,  G01N33/5088 ,  G01N33/5091

Abstract: A system is provided that simulates the in vivo micro-environment of three-dimensional cellular structures or bodies, such as tumors. The system simulates the pressure gradients and fluid flows of the vascular and lymphatic systems as well as the interstitial and capillary transport mechanisms between the 3D cellular structure and the vascular and lymphatic systems. The system can be used to introduce drugs or drug delivery carriers to a tumor, for example, to assess the uptake capability and effect on the tumor. The system maintains the viability of the tumor cells for a sufficiently long period of time to permit testing of several different drugs and/or delivery carriers.

Abstract translation: 提供了一种模拟三维细胞结构或体内诸如肿瘤的体内微环境的系统。 该系统模拟血管和淋巴系统的压力梯度和流体流动以及3D细胞结构与血管和淋巴系统之间的间质和毛细血管转运机制。 该系统可用于将药物或药物递送载体引入肿瘤，例如以评估对肿瘤的摄取能力和作用。 该系统维持肿瘤细胞的活力足够长的时间以允许测试几种不同的药物和/或递送载体。

公开(公告)号：US20130295601A1

公开(公告)日：2013-11-07

申请号：US13886810

申请日：2013-05-03

Applicant: PURDUE RESEARCH FOUNDATION

Inventor： Kinam Park ,  Bumsoo Han ,  Bongseop Kwak ,  Crystal Soo Jung Shin

IPC: G01N33/50

CPC classification number: G01N33/5011 ,  B01L3/5027 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/163 ,  C12M21/08 ,  C12M25/04 ,  G01N33/5026 ,  G01N33/5088 ,  G01N33/5091

Abstract: A system is provided that simulates the in vivo micro-environment of three-dimensional cellular structures or bodies, such as tumors. The system simulates the pressure gradients and fluid flows of the vascular and lymphatic systems as well as the interstitial and capillary transport mechanisms between the 3D cellular structure and the vascular and lymphatic systems. The system can be used to introduce drugs or drug delivery carriers to a tumor, for example, to assess the uptake capability and effect on the tumor. The system maintains the viability of the tumor cells for a sufficiently long period of time to permit testing of several different drugs and/or delivery carriers.

Abstract translation: 提供了一种模拟三维细胞结构或体内诸如肿瘤的体内微环境的系统。 该系统模拟血管和淋巴系统的压力梯度和流体流动以及3D细胞结构与血管和淋巴系统之间的间质和毛细血管转运机制。 该系统可用于将药物或药物递送载体引入肿瘤，例如以评估对肿瘤的摄取能力和作用。 该系统维持肿瘤细胞的活力足够长的时间以允许测试几种不同的药物和/或递送载体。