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    Microfabricated integrated DNA analysis system
    4.
    发明申请
    Microfabricated integrated DNA analysis system 有权
    微型综合DNA分析系统

    公开(公告)号：US20050287572A1

    公开(公告)日：2005-12-29

    申请号：US11139018

    申请日：2005-05-25

    申请人： Richard Mathies ,  Robert Blazej ,  Chung Liu ,  Palani Kumaresan ,  Stephanie Yeung

    发明人： Richard Mathies ,  Robert Blazej ,  Chung Liu ,  Palani Kumaresan ,  Stephanie Yeung

    IPC分类号： B01L3/00 ,  B01L7/00 ,  C12M1/34 ,  C12Q1/68

    CPC分类号： B01L3/50273 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2300/0803 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0633 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q2565/518 ,  C12Q2537/143 ,  C12Q2565/629

    摘要： Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments. The microfabricated structure may be used in a process for performing sequencing and other genetic analysis of DNA or RNA.

    摘要翻译： 提供了基因组分析的方法和装置。 包括微流体分配通道的微制造结构被配置为将具有测序模板的拷贝的微反应器元件分配到多个微制造的热循环室中。 微反应器元件可以包括承载测序模板的多个拷贝的微载体元件。 微载体元件可以包含微球体。 可以使用热循环室，光学扫描器或定时装置的出口处的自动阀，以确保仅一个微球将流入一个热循环室，其中产生热循环延伸碎片。 延伸产物被捕获，纯化和浓缩在一个整合的寡核苷酸凝胶捕获室中。 微量组分分离装置用于分析纯化的延伸片段。 微制造结构可用于进行DNA或RNA的测序和其他遗传分析的过程。

    Detection methods using probes labeled with energy transfer coupled dyes for DNA fragment analysis
    5.
    发明授权
    Detection methods using probes labeled with energy transfer coupled dyes for DNA fragment analysis 有权
    使用标记有能量转移偶联染料的探针进行DNA片段分析的检测方法

    公开(公告)号：US06177247B1

    公开(公告)日：2001-01-23

    申请号：US09201078

    申请日：1998-11-30

    申请人： Richard Mathies ,  Alexander Glazer ,  Jingyue Ju

    发明人： Richard Mathies ,  Alexander Glazer ,  Jingyue Ju

    IPC分类号： C12Q168

    CPC分类号： G01N33/542 ,  C12Q1/6818 ,  C12Q1/6869 ,  C12Q2537/143 ,  C12Q2563/107

    摘要： Compositions are provided comprising sets of fluorescent labeled oligonucleotides carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donors at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility and enhanced emission intensities under separation conditions, by varying the distance between the donor and acceptor in a given pair. Particularly, the fluorescent compositions find use as labels in analyzing double stranded and single stranded nucleic acid fragments using capillary electrophoresis.

    摘要翻译： 提供的组合物包括携带供体和受体染料分子对的荧光标记寡核苷酸组，设计用于在单个波长下有效激发供体，并以不同波长的每对中的受体发射。 具有不同供体 - 受体对的不同分子可以通过改变在给定对中的供体和受体之间的距离而在分离条件下具有基本上相同的迁移率和增强的发射强度。 特别地，荧光组合物用于使用毛细管电泳分析双链和单链核酸片段的标签。

    Primers labeled with energy transfer coupled dyes for DNA sequencing
    6.
    发明授权
    Primers labeled with energy transfer coupled dyes for DNA sequencing 失效
    用能量转移偶联染料标记用于DNA测序的引物

    公开(公告)号：US5707804A

    公开(公告)日：1998-01-13

    申请号：US410808

    申请日：1995-03-27

    申请人： Richard Mathies ,  Alexander Glazer ,  Jingyue Ju

    发明人： Richard Mathies ,  Alexander Glazer ,  Jingyue Ju

    IPC分类号： G01N33/53 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/542 ,  G01N33/566 ,  G01N33/58 ,  C07H21/04

    CPC分类号： G01N33/582 ,  G01N33/542 ,  Y10S435/81

    摘要： Compositions are provided for making oligonucleotides carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donor at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair, and to have enhanced emission intensities from the acceptor. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.

    摘要翻译： 提供组合物用于制备携带供体和受体染料分子对的寡核苷酸，设计用于在单个波长下有效激发供体，并以不同波长的每对中的受体发射。 具有不同供体 - 受体对的不同分子可以通过改变给定对中的供体和受体之间的距离并且具有来自受体的增强的发射强度而在分离条件下具有基本上相同的迁移率。 特别地，荧光组合物在测序核酸中可用作标记。

    Probes labeled with energy transfer coupled dyes
    10.
    发明授权
    Probes labeled with energy transfer coupled dyes 失效
    用能量转移偶联染料标记的探针

    公开(公告)号：US06544744B1

    公开(公告)日：2003-04-08

    申请号：US09819050

    申请日：2000-01-20

    申请人： Richard Mathies ,  Alexander Glazer ,  Jingyue Ju

    发明人： Richard Mathies ,  Alexander Glazer ,  Jingyue Ju

    IPC分类号： C12Q168

    CPC分类号： G01N33/582 ,  C12Q1/6818 ,  C12Q1/6869 ,  G01N33/542 ,  C12Q2537/143 ,  C12Q2565/101 ,  C12Q2535/101 ,  C12Q2563/107 ,  C12Q2565/1015

    摘要： Fluorescent labels having at least one donor and at least one acceptor fluorophore bonded to a polymeric backbone in energy transfer relationship, as well as methods for their use, are provided. Of particular interest are the subject labels wherein the polymeric backbone is a nucleic acid and the donor fluorophore is bonded to the 5′ terminus of said nucleic acid. Such labels find use as primers in applications involving nucleic acid chain extension, such as sequencing, PCR and the like.

    摘要翻译： 提供具有至少一个供体和至少一个受体荧光团的荧光标记，其与能量转移关系中的聚合物主链结合，以及它们的使用方法。 特别感兴趣的是其中聚合物主链是核酸并且供体荧光团与所述核酸的5'末端键合的主题标签。 这样的标记在涉及核酸链延伸的应用（例如测序，PCR等）中用作引物。