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    Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions
    5.
    发明授权
    Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions 失效
    血清学检测艾滋病和艾滋病前期患者血清中HTLV-III的抗体

    公开(公告)号：US4520113A

    公开(公告)日：1985-05-28

    申请号：US602945

    申请日：1984-04-23

    申请人： Robert C. Gallo ,  Mikulas Popovic ,  Mangalasseril G. Sarngadharan

    发明人： Robert C. Gallo ,  Mikulas Popovic ,  Mangalasseril G. Sarngadharan

    IPC分类号： G01N33/543 ,  A61K39/00 ,  C12N7/04 ,  C12Q1/70 ,  G01N33/569 ,  G01N33/54 ,  G01N33/56

    CPC分类号： G01N33/56988 ,  Y10S435/81 ,  Y10S435/948 ,  Y10S435/974 ,  Y10S435/975 ,  Y10S436/804 ,  Y10S436/808 ,  Y10S436/809 ,  Y10S436/811

    摘要： This invention relates to the detection of antibodies in sera of AIDS and pre-AIDS patients and describes the biochemical and immunological analysis of antigens associated with the virus HTLV-III Human T-Cell Leukemia Virus. It is shown that antigens associated with the infection of human cells by this virus are specifically recognized by antibodies from AIDS patients. Specifically, HTLV-III isolated from AIDS patients and transmitted by cocultivation with an HT cell line is specifically detected by antibodies from human sera taken from AIDS patients. The method of detection of antibodies preferred is a strip radioimmunoassay (RIA) based on the Western Blot technique or an ELISA (an enzyme-linked immunosorbent assay) or an indirect immunofluorescence assay.

    摘要翻译： 本发明涉及艾滋病和艾滋病前期患者血清中抗体的检测，并描述了与病毒HTLV-III人T细胞白血病病毒相关的抗原的生物化学和免疫学分析。 显示出与该病毒感染人细胞相关的抗原由AIDS患者的抗体特异性地识别。 具体来说，艾滋病患者分离出来的并通过HT细胞系共培养传播的HTLV-III由来自艾滋病患者的人血清的抗体特异性检测。 检测抗体的方法优选是基于Western Blot技术或ELISA（酶联免疫吸附测定）或间接免疫荧光测定的条带放射免疫测定（RIA）。

    Monoclonal antibodies for binding HTLV-III proteins, and cell lines for their production
    6.
    发明授权
    Monoclonal antibodies for binding HTLV-III proteins, and cell lines for their production 失效
    用于结合HTLV-III蛋白的单克隆抗体，以及用于其生产的细胞系

    公开(公告)号：US4843011A

    公开(公告)日：1989-06-27

    申请号：US816573

    申请日：1986-01-03

    申请人： Mangalasseril G. Sarngadharan ,  Fulvia di Marzo Veronese ,  Robert C. Gallo

    发明人： Mangalasseril G. Sarngadharan ,  Fulvia di Marzo Veronese ,  Robert C. Gallo

    IPC分类号： C07K16/10

    CPC分类号： C07K16/1063 ,  C07K16/1054 ,  Y10S435/81 ,  Y10S436/808 ,  Y10S436/809 ,  Y10S436/811 ,  Y10S530/808 ,  Y10S530/809 ,  Y10S530/864 ,  Y10S930/221

    摘要： Monoclonal antibodies, and hybridoma cell lines for their production, that bind with a high degree of specificity proteins associated with HTLV-III virus are presently disclosed. In particular, transmembrane envelope glycoprotein gp41 (41,000 dalton molecular size), major core antigen p24 (24,000 dalton molecular size), and p17 protein (17,000 dalton molecular size) are disclosed. The proteins to which the present monoclonal antibodies respond are essentially antigenically distinct from HTLV-I and HTLV-II. SVM-16 is an IgM monoclonal antibody, SVM-23 is an IgG.sub.2 monoclonal antibody, and SVM-26 is an IgG.sub.1 monoclonal antibody, all of which bind to p24. SVM-25 is an IgG.sub.1 monoclonal antibody binding gp41, and SVM-33 is an IgG.sub.1 monoclonal antibody binding p17. All the monoclonal antibodies of the present invention are produced in hybridoma cells prepared by fusing myeloma cells with spleen cells from mammals, such as mice, immunized with lysates of purified virus.

    摘要翻译： 目前公开了与HTLV-III病毒相关的高度特异性蛋白质结合的用于其生产的单克隆抗体和杂交瘤细胞系。 具体地，公开了跨膜包膜糖蛋白gp41（41,000道尔顿分子大小），主要核心抗原p24（24,000道尔顿分子大小）和p17蛋白（17,000道尔顿分子大小）。 本单克隆抗体应答的蛋白质本质上与HTLV-1和HTLV-II具有抗原性。 SVM-16是IgM单克隆抗体，SVM-23是IgG2单克隆抗体，SVM-26是IgG1单克隆抗体，均与p24结合。 SVM-25是结合gp41的IgG1单克隆抗体，SVM-33是结合p17的IgG1单克隆抗体。 将本发明的所有单克隆抗体产生于通过将骨髓瘤细胞与来自哺乳动物（例如用纯化病毒的裂解物免疫的）的小鼠的脾细胞融合而制备的杂交瘤细胞中。

    Method of continuous production of retroviruses (HTLV-III) from patients with AIDS and pre-AIDS using permissive cells
    8.
    发明授权
    Method of continuous production of retroviruses (HTLV-III) from patients with AIDS and pre-AIDS using permissive cells 失效
    使用允许细胞从艾滋病和艾滋病患者连续生产逆转录病毒（HTLV-III）的方法

    公开(公告)号：US4652599A

    公开(公告)日：1987-03-24

    申请号：US643729

    申请日：1984-08-24

    申请人： Robert C. Gallo ,  Mikulas Popovic

    发明人： Robert C. Gallo ,  Mikulas Popovic

    IPC分类号： A61K39/21 ,  A61P31/12 ,  A61P31/18 ,  C12N5/06 ,  C12N7/00 ,  C12R1/91 ,  C12R1/92 ,  G01N33/569 ,  C12N7/02 ,  C12N5/00 ,  C12Q1/02 ,  C12Q1/70

    CPC分类号： C12N7/00 ,  C12N2740/10051 ,  Y10S435/948

    摘要： A cell system is disclosed for the reproducible detection and isolation of human T-lymphotropic retroviruses (HTLV-family) with cytopathic effects (HTLV-III) from patients with the acquired immune deficiency syndrome (AIDS), pre-AIDS and in healthy carriers. One neoplastic aneuploid T-cell line derived from an adult with lymphoid leukemia, and termed HT, was susceptible to infection with HTLV-III, which is transformed and provides T-cell populations which are highly susceptible to and permissive for HTLV-III, and convenient for large scale production, isolation, and biological detection of the virus. Other operational neoplastic T-cell lines which are positive for OKT4 marker, e.g., Molt 3, CEM, Ti7.4 and HUT78, can produce HTLV-III in a large amount and retain its unlimited capability for growth.

    摘要翻译： 公开了一种细胞系统，用于从具有获得性免疫缺陷综合征（AIDS），艾滋病前期和健康携带者的患者中检测和分离具有细胞病变效应（HTLV-III）的人类T淋巴细胞逆转录病毒（HTLV家族）。 衍生自具有淋巴样白血病，称为HT的成年人的一种肿瘤非整倍体T细胞系易感染HTLV-III，HTLV-III被转化并提供对HTLV-III高度敏感和允许的T细胞群体，以及 方便大规模生产，隔离和生物检测病毒。 对于OKT4标志物，例如Molt 3，CEM，Ti7.4和HUT78而言为阳性的其他可操作的肿瘤性T细胞系可以大量产生HTLV-III并保持其无限的生长能力。

    Method of continuous production of retroviruses (HTLV-III) from patients with AIDS and pre-AIDS
    9.
    发明授权
    Method of continuous production of retroviruses (HTLV-III) from patients with AIDS and pre-AIDS 失效
    从艾滋病和艾滋病前期继续生产逆转录病毒（HTLV-III）的方法

    公开(公告)号：US4647773A

    公开(公告)日：1987-03-03

    申请号：US602946

    申请日：1984-04-23

    申请人： Robert C. Gallo ,  Mikulas Popovic

    发明人： Robert C. Gallo ,  Mikulas Popovic

    IPC分类号： G01N33/50 ,  A61K39/21 ,  A61P31/12 ,  A61P31/18 ,  C12N5/00 ,  C12N5/09 ,  C12N7/00 ,  C12R1/91 ,  C12R1/92 ,  G01N33/569 ,  C12N7/02

    CPC分类号： C12N5/0694 ,  C12N7/00 ,  C12N2740/16051 ,  Y10S435/948

    摘要： A cell system is disclosed for the reproducible detection and isolation of human T-lymphotropic retroviruses (HTLV-family) with cytopathic effects (HTLV-III) from patients with the acquired immune deficiency syndrome (AIDS), pre-AIDS and in healthy carriers. One neoplastic aneuploid T-cell line derived from an adult with lymphoid leukemia, and termed HT, was susceptible to infection with the new variants of HTLV, which are transformed and providing T-cell populations which are highly susceptible and permissive from HTLV-III, and convenience for large scale production, isolation and biological detection of the virus.

    摘要翻译： 公开了一种细胞系统，用于从具有获得性免疫缺陷综合征（AIDS），艾滋病前期和健康携带者的患者中检测和分离具有细胞病变效应（HTLV-III）的人类T淋巴细胞逆转录病毒（HTLV家族）。 衍生自具有淋巴样白血病，称为HT的成年人的一种肿瘤非整倍体T细胞系易感染HTLV的新变体，其被转化并提供从HTLV-III高度敏感和允许的T细胞群体， 并方便大规模生产，分离和生物检测病毒。