-
1.发明授权Systems and methods for predicting oligonucleotide melting temperature (TmS) 有权用于预测寡核苷酸融合温度（TmS）的系统和方法公开(公告)号：US07715989B2
公开(公告)日：2010-05-11
申请号：US10176972
申请日：2002-06-18
申请人： Robert O. Dempcy , Alexander A. Gall , Sergey G. Lokhov , Irina A. Afonina , Michael J. Singer , Karen M. Singer, legal representative , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
发明人： Robert O. Dempcy , Alexander A. Gall , Sergey G. Lokhov , Irina A. Afonina , Michael J. Singer , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
IPC分类号： G01N33/48
CPC分类号： G06F19/20 , C07B2200/11 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H19/23 , C07H21/00 , C12Q1/6813 , C12Q1/6827 , C12Q1/6858 , C12Q2600/156 , C40B40/00 , G06F19/22 , C12Q2563/173 , C12Q2527/107 , C12Q2525/117 , C12Q2525/197 , C12Q2541/101 , C12Q2535/125
摘要： Software systems and methods for predicting the melting temperature (Tm) and other characteristics of oligonucleotides, including modified oligonucleotides. Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
摘要翻译： 用于预测寡核苷酸的融解温度（Tm）和其他特征的软件系统和方法，包括修饰的寡核苷酸。 提供了修饰的寡核苷酸，其含有选自未取代的和3-取代的吡唑并[3,4-d]嘧啶和5-取代的嘧啶的碱，并且任选具有连接的小沟结合剂和报道基团。
-
公开(公告)号：US06683173B2
公开(公告)日：2004-01-27
申请号：US10032307
申请日：2001-12-21
申请人： Robert O. Dempcy , Alexander A. Gall , Sergey G. Lokhov , Irina A. Afonina , Michael J. Singer , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
发明人： Robert O. Dempcy , Alexander A. Gall , Sergey G. Lokhov , Irina A. Afonina , Michael J. Singer , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
IPC分类号： C07H2104
CPC分类号： C07H21/00 , C07B2200/11 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H19/23 , C12Q1/6832 , C40B40/00 , G06F19/22 , C12Q2525/117
摘要： Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
摘要翻译： 提供了修饰的寡核苷酸，其含有选自未取代的和3-取代的吡唑并[3,4-d]嘧啶和5-取代的嘧啶的碱，并且任选具有连接的小沟结合剂和报道基团。
-
公开(公告)号：US07045610B2
公开(公告)日：2006-05-16
申请号：US09796988
申请日：2001-02-28
申请人： Robert O. Dempcy , Alexander A. Gall , Sergey G. Lokhov , Irina A. Afonina , Michael J. Singer , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
发明人： Robert O. Dempcy , Alexander A. Gall , Sergey G. Lokhov , Irina A. Afonina , Michael J. Singer , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
CPC分类号： C07H19/06 , C07B2200/11 , C07H19/10 , C07H19/16 , C07H19/20 , C07H19/23 , C07H21/00 , C12Q1/6832 , C40B40/00 , G06F19/22 , C12Q2525/117
摘要： Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
摘要翻译： 提供了修饰的寡核苷酸，其含有选自未取代的和3-取代的吡唑并[3,4-d]嘧啶和5-取代的嘧啶的碱，并且任选具有连接的小沟结合剂和报道基团。
-
公开(公告)号：US07368549B2
公开(公告)日：2008-05-06
申请号：US11109923
申请日：2005-04-19
申请人： Robert O. Dempcy , Alexander A Gall , Sergey G. Lokhov , Irina A. Afonina , Michael J. Singer , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
发明人： Robert O. Dempcy , Alexander A Gall , Sergey G. Lokhov , Irina A. Afonina , Michael J. Singer , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
CPC分类号： C07H19/06 , C07B2200/11 , C07H19/10 , C07H19/16 , C07H19/20 , C07H19/23 , C07H21/00 , C12Q1/6832 , C40B40/00 , G06F19/22 , C12Q2525/117
摘要： Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
摘要翻译： 提供了修饰的寡核苷酸，其含有选自未取代的和3-取代的吡唑并[3,4-d]嘧啶和5-取代的嘧啶的碱，并且任选具有连接的小沟结合剂和报道基团。
-
公开(公告)号：US07751982B2
公开(公告)日：2010-07-06
申请号：US10672429
申请日：2003-09-26
申请人： Robert O. Dempcy , Alexander A. Gall , Sergey G. Lokhov , Irina A. Afonina , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
发明人： Robert O. Dempcy , Alexander A. Gall , Sergey G. Lokhov , Irina A. Afonina , Igor V. Kutyavin , Nicolaas M. J. Vermeulen
CPC分类号： C07H21/00 , C07B2200/11 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H19/23 , C12Q1/6832 , C40B40/00 , G06F19/22 , C12Q2525/117
摘要： Methods for designing an oligonucleotide sequence having a selected duplex stability are provided wherein the oligonucleotide portion comprises at least one modified base selected from universal bases, unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally having attached minor groove binders and reporter groups.
摘要翻译： 提供了设计具有所选双链体稳定性的寡核苷酸序列的方法，其中寡核苷酸部分包含至少一个选自通用碱基，未取代的和3-取代的吡唑并[3,4-d]嘧啶和5-取代的嘧啶的修饰碱基， 附有小沟结合物和记者组。
-
6.发明授权Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for PCR analysis 有权实时线性检测探针：用于PCR分析的敏感的5'-小沟粘合剂探针公开(公告)号：US07205105B2
公开(公告)日：2007-04-17
申请号：US10165410
申请日：2002-06-06
申请人： Irina A. Afonina , Yevgeniy S. Belousov , Robert O. Dempcy , Igor V. Kutyavin , Sergey G. Lokhov , Eugeny A. Lukhtanov
发明人： Irina A. Afonina , Yevgeniy S. Belousov , Robert O. Dempcy , Igor V. Kutyavin , Sergey G. Lokhov , Eugeny A. Lukhtanov
CPC分类号： C07C245/08 , C07B2200/11 , C07D519/00 , C07F9/2408 , C07F9/572 , C07F9/65522 , C07F9/6561 , C07H21/00 , C09B29/0813 , C12Q1/6818 , C12Q1/6844 , C12Q1/686 , C40B40/00 , C12Q2565/1015 , C12Q2563/173 , C12Q2563/107 , C12Q2545/101 , C12Q2561/113 , C12Q2525/197 , C12Q2565/1025 , C12Q2521/319 , C12Q2565/107 , C12Q2525/125
摘要： Oligonucleotide probes/conjugates are provided along with method for their use in assays to monitor amplification wherein the signal produced does not rely on 5′ nuclease digestion.
摘要翻译： 提供寡核苷酸探针/缀合物及其用于监测扩增的测定法的方法，其中产生的信号不依赖于5'核酸酶消化。
-
7.发明授权Real-time linear detection probes: sensitive 5'-minor groove binder-containing probes for PCR analysis 有权实时线性检测探针：用于PCR分析的敏感的5'-小沟粘合剂探针公开(公告)号：US07485442B2
公开(公告)日：2009-02-03
申请号：US11471292
申请日：2006-06-19
申请人： Irina A. Afonina , Yevgeniy S. Belousov , Robert O. Dempcy , Igor V. Kutyavin , Sergey G. Lokhov , Eugeny A. Lukhtanov
发明人： Irina A. Afonina , Yevgeniy S. Belousov , Robert O. Dempcy , Igor V. Kutyavin , Sergey G. Lokhov , Eugeny A. Lukhtanov
CPC分类号： C07C245/08 , C07B2200/11 , C07D519/00 , C07F9/2408 , C07F9/572 , C07F9/65522 , C07F9/6561 , C07H21/00 , C09B29/0813 , C12Q1/6818 , C12Q1/6844 , C12Q1/686 , C40B40/00 , C12Q2565/1015 , C12Q2563/173 , C12Q2563/107 , C12Q2545/101 , C12Q2561/113 , C12Q2525/197 , C12Q2565/1025 , C12Q2521/319 , C12Q2565/107 , C12Q2525/125
摘要： Oligonucleotide probes/conjugates are provided along with method for their use in assays to monitor amplification wherein the signal produced does not rely on 5′ nuclease digestion.
摘要翻译： 提供寡核苷酸探针/缀合物及其用于监测扩增的测定法的方法，其中产生的信号不依赖于5'核酸酶消化。
-
8.发明授权Non-aggregating non-quenching oligomer comprising nucelotide analogs, method of synthesis and use thereof 有权包含芥菜素类似物的非聚集性非猝灭寡聚物，其合成方法和用途公开(公告)号：US06972328B2
公开(公告)日：2005-12-06
申请号：US10702007
申请日：2003-11-04
IPC分类号： G01N33/53 , A61K31/519 , A61K48/00 , C07D487/04 , C07H21/00 , C07H21/04 , C07K5/06 , C07K5/078 , C07K14/00 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N33/50 , G01N33/58 , C07H21/02
CPC分类号： C07D487/04 , A61K31/519 , C07H21/00 , C07K5/06026 , C07K5/06139 , C07K14/003 , C12Q1/6818 , C12Q1/6832 , C12Q2525/107 , C12Q2525/101
摘要： The invention provides compositions and methods for improved hybridization analysis utilizing DNA, RNA, PNA and chimeric oligomers in which one or more purine bases are substituted by a pyrazolo[5,4-d]pyrimidine or by a 7-deazapurine purine analogue. Reduced self-aggregation and reduced fluorescence quenching are obtained when the oligomers are used in various methods involving hybridization. Methods of synthesis, as well as novel synthetic precursors, are also provided.
摘要翻译： 本发明提供使用DNA，RNA，PNA和嵌合寡聚物改进杂交分析的组合物和方法，其中一个或多个嘌呤碱基被吡唑并[5,4-d]嘧啶或7-脱氮嘌呤嘌呤类似物取代。 当寡聚物以涉及杂交的各种方法使用时，获得了减少的自聚集和减少的荧光猝灭。 还提供了合成方法，以及新型合成前体。
-
9.发明授权Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof 有权包含核苷酸类似物的非聚集性非猝灭性低聚物; 合成和使用方法公开(公告)号：US06660845B1
公开(公告)日：2003-12-09
申请号：US09447936
申请日：1999-11-23
IPC分类号： C07H2100
CPC分类号： C07D487/04 , A61K31/519 , C07H21/00 , C07K5/06026 , C07K5/06139 , C07K14/003 , C12Q1/6818 , C12Q1/6832 , C12Q2525/107 , C12Q2525/101
摘要： The invention provides compositions and methods for improved hybridization analysis utilizing DNA, RNA, PNA and chimeric oligomers in which one or more purine bases are substituted by a pyrazolo[5,4-d]pyrimidine or by a 7-deazapurine purine analogue. Reduced self-aggregation and reduced fluorescence quenching are obtained when the oligomers are used in various methods involving hybridization. Methods of synthesis, as well as novel synthetic precursors, are also provided.
摘要翻译： 本发明提供使用DNA，RNA，PNA和嵌合寡聚物改进杂交分析的组合物和方法，其中一个或多个嘌呤碱基被吡唑并[5,4-d]嘧啶或7-脱氮嘌呤嘌呤类似物取代。 当寡聚物以涉及杂交的各种方法使用时，获得了减少的自聚集和减少的荧光猝灭。 还提供了合成方法，以及新型合成前体。
-
公开(公告)号：US06949367B1
公开(公告)日：2005-09-27
申请号：US09724959
申请日：2000-11-28
CPC分类号： C07H21/02
摘要： Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
摘要翻译： 提供了修饰的寡核苷酸，其含有选自未取代的和3-取代的吡唑并[3,4-d]嘧啶和5-取代的嘧啶的碱，并且任选具有连接的小沟结合剂和报道基团。
-
-
-
-
-
-
-
-
-
搜索结果
38 条记录 (耗时 0.051 秒)
