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    Asymmetric PCR amplification, its special primer and application
    4.
    发明授权
    Asymmetric PCR amplification, its special primer and application 有权
    不对称PCR扩增，其特殊引物及应用

    公开(公告)号：US08735067B2

    公开(公告)日：2014-05-27

    申请号：US11661069

    申请日：2004-11-22

    申请人： Zhiwei Zhang ,  Can Wang ,  Lingxiang Zhu ,  Qiong Zhang ,  Jing Cheng

    发明人： Zhiwei Zhang ,  Can Wang ,  Lingxiang Zhu ,  Qiong Zhang ,  Jing Cheng

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/686 ,  C12Q2531/107 ,  C12Q2525/155 ,  C12Q2525/15

    摘要： The present invention discloses an asymmetric PCR amplification method, its special primer and application, aims to provide a simple, effective PCR amplification for preparation of single-stranded product. The asymmetric PCR primer of the invention comprises some PCR primer pairs, in which an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one primer. The asymmetric PCR amplification provided includes the steps: 1) preparative denaturing; 2) repetitiously denaturing, primers annealing, extending cycles as the first stage of PCR amplification; 3) repetitiously denaturing, primer extending cycles as the second stage of PCR amplification, wherein an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one PCR primer of each pair in extension. With the asymmetric PCR amplification of the invention, high throughput of single-stranded products can be obtained, single PCR amplification or multiple PCR amplification can be carried out. And the method can be widely used in detection of nucleic acids.

    摘要翻译： 本发明公开了一种不对称PCR扩增方法及其特异性引物及其应用，旨在为单链产物的制备提供简单有效的PCR扩增。 本发明的不对称PCR引物包含一些PCR引物对，其中将待检测的靶序列的不相关核酸序列添加到一个引物的5'末端。 提供的不对称PCR扩增包括以下步骤：1）制备型变性; 2）重复变性，引物退火，延伸循环作为PCR扩增的第一阶段; 3）重复变性，引物延伸循环作为PCR扩增的第二阶段，其中将待检测的靶序列的不相关核酸序列添加到每对扩增中的一个PCR引物的5'末端。 利用本发明的不对称PCR扩增，可以获得高通量的单链产物，可进行单PCR扩增或多重PCR扩增。 该方法可广泛用于检测核酸。

    Asymmetric PCR Amplification, its Special Primer and Application
    5.
    发明申请
    Asymmetric PCR Amplification, its Special Primer and Application 有权
    不对称PCR扩增，其特殊引物和应用

    公开(公告)号：US20100151448A1

    公开(公告)日：2010-06-17

    申请号：US11661069

    申请日：2004-11-22

    申请人： Zhiwei Zhang ,  Can Wang ,  Lingxiang Zhu ,  Qiong Zhang ,  Jing Cheng

    发明人： Zhiwei Zhang ,  Can Wang ,  Lingxiang Zhu ,  Qiong Zhang ,  Jing Cheng

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/686 ,  C12Q2531/107 ,  C12Q2525/155 ,  C12Q2525/15

    摘要： The present invention discloses an asymmetric PCR amplification method, its special primer and application, aims to provide a simple, effective PCR amplification for preparation of single-stranded product. The asymmetric PCR primer of the invention comprises some PCR primer pairs, in which an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one primer. The asymmetric PCR amplification provided includes the steps: 1) preparative denaturing; 2) repetitiously denaturing, primers annealing, extending cycles as the first stage of PCR amplification; 3) repetitiously denaturing, primer extending cycles as the second stage of PCR amplification, wherein an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one PCR primer of each pair in extension. With the asymmetric PCR amplification of the invention, high throughput of single-stranded products can be obtained, single PCR amplification or multiple PCR amplification can be carried out. And the method can be widely used in detection of nucleic acids.

    摘要翻译： 本发明公开了一种不对称PCR扩增方法及其特异性引物及其应用，旨在为单链产物的制备提供简单有效的PCR扩增。 本发明的不对称PCR引物包含一些PCR引物对，其中将待检测的靶序列的不相关核酸序列添加到一个引物的5'末端。 提供的不对称PCR扩增包括以下步骤：1）制备型变性; 2）重复变性，引物退火，延伸循环作为PCR扩增的第一阶段; 3）重复变性，引物延伸循环作为PCR扩增的第二阶段，其中将待检测的靶序列的不相关核酸序列添加到每对扩增中的一个PCR引物的5'末端。 利用本发明的不对称PCR扩增，可以获得高通量的单链产物，可进行单PCR扩增或多重PCR扩增。 该方法可广泛用于检测核酸。

    Trajectory correction method and apparatus for k-space data in magnetic resonance imaging
    6.
    发明授权
    Trajectory correction method and apparatus for k-space data in magnetic resonance imaging 有权
    磁共振成像中k空间数据的轨迹校正方法和装置

    公开(公告)号：US09594146B2

    公开(公告)日：2017-03-14

    申请号：US13927376

    申请日：2013-06-26

    申请人： De He Weng ,  Qiong Zhang

    发明人： De He Weng ,  Qiong Zhang

    IPC分类号： G01R35/00 ,  G01R33/565 ,  G01R33/48

    CPC分类号： G01R35/005 ,  G01R33/482 ,  G01R33/4822 ,  G01R33/4824 ,  G01R33/565

    摘要： In a trajectory correction method and apparatus for k-space data points in magnetic resonance imaging, a magnetic resonance data acquisition unit is operated to execute a sampling sequence to obtain k-space to be corrected. Empirical points are selected used to divide k-space to be corrected into a central region and a peripheral region. The trajectories of the data points in the central region and the peripheral region are corrected and the sampling sequence is executed again, with the corrected trajectories, to obtain corrected k-space.

    摘要翻译： 在用于磁共振成像中的k空间数据点的轨迹校正方法和装置中，操作磁共振数据获取单元以执行采样序列以获得要校正的k空间。 经验点被选择用于将要校正的k空间划分为中心区域和外围区域。 校正中心区域和外围区域中的数据点的轨迹，并且利用校正的轨迹再次执行采样序列，以获得校正的k空间。

    System and method for shared mesh restoration in optical networks
    7.
    发明授权
    System and method for shared mesh restoration in optical networks 有权
    光网络中共享网格恢复的系统和方法

    公开(公告)号：US08965198B2

    公开(公告)日：2015-02-24

    申请号：US13658587

    申请日：2012-10-23

    申请人： Qiong Zhang ,  Paparao Palacharla ,  Xi Wang ,  Motoyoshi Sekiya

    发明人： Qiong Zhang ,  Paparao Palacharla ,  Xi Wang ,  Motoyoshi Sekiya

    IPC分类号： H04B10/00

    CPC分类号： H04J14/0284 ,  H04J14/0212 ,  H04J14/0217 ,  H04J14/0268

    摘要： A method for shared mesh restoration includes configuring a switch to allow sharing of a plurality of backup line cards across a plurality of node degrees associated with a reconfigurable optical add/drop multiplexer (ROADM). The switch is communicatively coupled to the ROADM. The method further includes configuring a number of backup line cards coupled to the switch. The number of backup line cards is based on determining a number of active backup lightpaths for each of a plurality of network failures associated with each of the plurality of node degrees of the ROADM, identifying which node degree and failure has the largest number of active backup lightpaths for all of the plurality of node degrees of the ROADM and for each of the plurality of network failures, and determining the number of backup line cards to configure based on the identified largest number of active backup lightpaths.

    摘要翻译： 一种用于共享网格恢复的方法包括配置交换机以允许在与可重配置光分插复用器（ROADM）相关联的多个节点度上共享多个备用线卡。 交换机通信耦合到ROADM。 该方法还包括配置耦合到交换机的多个备用线路卡。 备用线路卡的数量基于确定与ROADM的多个节点度中的每一个相关联的多个网络故障中的每一个的主动备用光路的数量，识别哪个节点度和故障具有最大数量的主动备份 用于ROADM的所有多个节点度数和多个网络故障中的每一个的光路，以及基于所识别的最大数量的主动备用光路确定要配置的备用线路卡的数量。

    System and method for calculating utilization entropy
    8.
    发明授权
    System and method for calculating utilization entropy 有权
    计算利用熵的系统和方法

    公开(公告)号：US08942114B2

    公开(公告)日：2015-01-27

    申请号：US13165352

    申请日：2011-06-21

    申请人： Xi Wang ,  Inwoong Kim ,  Qiong Zhang ,  Paparao Palacharla ,  Motoyoshi Sekiya

    发明人： Xi Wang ,  Inwoong Kim ,  Qiong Zhang ,  Paparao Palacharla ,  Motoyoshi Sekiya

    IPC分类号： H04L12/26 ,  H04L12/28

    CPC分类号： H04L43/0882

    摘要： A system and method are provided for monitoring traffic in a network comprising a plurality of links, wherein each of the plurality of links comprises a plurality of neighboring pairs of slots. The system and method may include identifying a first usage status and a second usage status, calculating a utilization entropy value based at least on the difference between the first and second usage status, iteratively calculating a set of utilization entropy values for a portion of the network, and calculating an overall utilization entropy value for the portion of the network under analysis based at least on a statistical analysis of the set of utilization entropy values.

    摘要翻译： 提供了一种用于监视包括多个链路的网络中的业务的系统和方法，其中所述多个链路中的每一个包括多个相邻的时隙对。 系统和方法可以包括识别第一使用状态和第二使用状态，至少基于第一和第二使用状态之间的差异来计算使用熵值，迭代地计算网络的一部分的一组利用熵值 以及至少基于所述利用熵值集合的统计分析来计算所分析的网络的部分的总利用熵值。

    MAGNETIC RESONANCE IMAGING METHOD
    9.
    发明申请
    MAGNETIC RESONANCE IMAGING METHOD 有权
    磁共振成像方法

    公开(公告)号：US20130342204A1

    公开(公告)日：2013-12-26

    申请号：US13923757

    申请日：2013-06-21

    申请人： Qiong Zhang ,  Cong Zhao

    发明人： Qiong Zhang ,  Cong Zhao

    IPC分类号： G01R33/54

    CPC分类号： G01R33/543 ,  G01R33/5607 ,  G01R33/5635 ,  G01R33/5676

    摘要： In a magnetic resonance imaging method and apparatus, navigation data are collected in a navigation acquisition timeslot of a scanning sequence. A determination as to whether to accept or reject echo data that are subsequently collected in multiple echo acquisition timeslots is made. If the phase relationship of the navigation data and the reference data in k-space is greater than or equal to the reference value, the collected echo data are accepted and if the aforementioned phase in k-space is less than the reference value, the collected echo data will be rejected, and sequential scanning will be performed again, and the navigation data again will be collected using the navigation acquisition timeslot, and the aforementioned determination is repeated.

    摘要翻译： 在磁共振成像方法和装置中，导航数据被收集在扫描序列的导航获取时隙中。 做出是否接受或拒绝随后在多个回波采集时隙中收集的回波数据的确定。 如果导航数据和k空间中的参考数据的相位关系大于或等于参考值，则接收收集的回波数据，如果k空间中的上述相位小于参考值，则收集 回波数据将被拒绝，并且将再次执行顺序扫描，并且将使用导航获取时隙再次收集导航数据，并且重复上述确定。