利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

19 条记录 (耗时 0.024 秒)

    Diagnostic compositions, elements, methods and test kits for amplification and detection of retroviral DNA using primers having matched melting temperatures
    1.
    发明授权
    Diagnostic compositions, elements, methods and test kits for amplification and detection of retroviral DNA using primers having matched melting temperatures 失效
    使用具有匹配熔融温度的引物扩增和检测逆转录病毒DNA的诊断组合物，元件，方法和测试试剂盒

    公开(公告)号：US5403707A

    公开(公告)日：1995-04-04

    申请号：US062022

    申请日：1993-05-14

    申请人： Susan M. Atwood ,  Thomas J. Cummins ,  John B. Findlay

    发明人： Susan M. Atwood ,  Thomas J. Cummins ,  John B. Findlay

    IPC分类号： C12Q1/68 ,  C12Q1/70 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/686 ,  C12Q1/6834

    摘要： An aqueous composition containing primers for opposing strands of a target retroviral DNA (such as HIV-I DNA) can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of that target DNA and one or more additional target DNA's. The primers for each target DNA differ in length by no more than 5 nucleotides and have a T.sub.m within the range of from about 65.degree. to about 74.degree. C., while all of the T.sub.m 's are within about 5.degree. C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in "multiplexing", using multiple capture probes, all of which have T.sub.m 's which are greater than 50.degree. C. and within 15.degree. C. of each other.

    摘要翻译： 可以在聚合酶链反应中使用含有针对靶向逆转录病毒DNA（例如HIV-1 DNA）的相对链的引物的含水组合物，以便同时快速且有效地扩增和检测该靶DNA和一种或多种另外的靶DNA。 每个靶DNA的引物长度不等于5个核苷酸，Tm在约65-74℃范围内，而所有Tm在彼此的约5℃内。 这样的组合物可用于诊断测试试剂盒和用于多重核酸的扩增和检测的方法，或使用多个捕获探针进行“多路复用”，所有这些探针具有大于50℃的Tm和15℃以内的Tm 彼此。

    Methods of extracting deoxyribonucleic acids without using a proteolytic enzyme
    3.
    发明授权
    Methods of extracting deoxyribonucleic acids without using a proteolytic enzyme 失效
    不使用蛋白水解酶提取脱氧核糖核酸的方法

    公开(公告)号：US5543305A

    公开(公告)日：1996-08-06

    申请号：US471806

    申请日：1995-06-06

    申请人： Thomas J. Cummins ,  Tobias D. Ekeze

    发明人： Thomas J. Cummins ,  Tobias D. Ekeze

    IPC分类号： C12N15/00 ,  C12Q1/68 ,  C12Q1/70 ,  C12P19/34 ,  C07H21/00

    CPC分类号： C12Q1/70 ,  C12Q1/6806 ,  C12Q1/6888 ,  C12Q1/703 ,  Y10S435/803 ,  Y10S435/81

    摘要： This invention provides a rapid and highly effective method for extracting nucleic acids from cells or virions without the use of proteolytic enzymes. Extraction is accomplished within a few minutes using a lysing composition comprising a buffer, a source of a DNA polymerase cofactor, a stabilizer and at least one nonionic surfactant which will release nucleic acids from cytoplasmic and nuclear membranes of cells or virions. The resulting mixture is heated to boiling for up to fifteen minutes, and the nucleic acids are recovered for amplification using polymerase chain reaction. No proteolytic enzyme is used in the extraction process.

    摘要翻译： 本发明提供了一种从不使用蛋白水解酶的细胞或病毒粒子中提取核酸的快速且高效的方法。 使用包含缓冲液，DNA聚合酶辅因子源，稳定剂和至少一种非离子表面活性剂的裂解组合物在几分钟内完成提取，其将从细胞或病毒粒子的细胞质和核膜释放核酸。 将所得混合物加热至沸腾达15分钟，并使用聚合酶链式反应回收核酸进行扩增。 在提取过程中不使用蛋白水解酶。

    Method, test element and test kit for semi-quantitative detection of target nucleic acid
    9.
    发明授权
    Method, test element and test kit for semi-quantitative detection of target nucleic acid 失效
    用于半定量检测靶核酸的方法，测试元件和测试试剂盒

    公开(公告)号：US5591580A

    公开(公告)日：1997-01-07

    申请号：US221818

    申请日：1994-03-31

    申请人： Lynn Bergmeyer ,  Thomas J. Cummins

    发明人： Lynn Bergmeyer ,  Thomas J. Cummins

    IPC分类号： G01N33/50 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/53 ,  C12P19/34

    CPC分类号： C12Q1/6834 ,  C12Q1/6813 ,  C12Q1/686

    摘要： A target nucleic acid can be detected in a semi-quantitative fashion by passing it over detection deposits in a test element. The detection deposits include particles affixed to the test element, some of which particles have capture probe attached thereto, and other particles having no capture probe. The deposits have varying amounts of capture probe so that the signal obtained when the target nucleic acid is captured thereon can be semi-quantitatively correlated to the amount of target nucleic acid in the specimen. This method of detection can be used in nucleic acid hybridization assays or following amplification methods, including polymerase chain reaction.

    摘要翻译： 靶核酸可以通过将其超过检测元件中的检测沉积物以半定量方式检测。 检测沉积物包括固定在测试元件上的颗粒，其中一些颗粒具有附着于其上的捕获探针和其它没有捕获探针的颗粒。 沉积物具有不同量的捕获探针，使得当靶核酸被捕获时获得的信号可以与样品中靶核酸的量半定量相关。 该检测方法可用于核酸杂交测定或扩增方法，包括聚合酶链反应。

    Element and method for nucleic acid amplification and detection using adhered probes
    10.
    发明授权
    Element and method for nucleic acid amplification and detection using adhered probes 失效
    使用粘附的探针进行核酸扩增和检测的元件和方法

    公开(公告)号：US5380489A

    公开(公告)日：1995-01-10

    申请号：US837772

    申请日：1992-02-18

    申请人： Richard C. Sutton ,  Ignazio S. Ponticello ,  Thomas J. Cummins ,  Dennis R. Zander ,  William H. Donish

    发明人： Richard C. Sutton ,  Ignazio S. Ponticello ,  Thomas J. Cummins ,  Dennis R. Zander ,  William H. Donish

    IPC分类号： B01L3/00 ,  C12Q1/68 ,  G01N33/543

    CPC分类号： B01L3/508 ,  C12Q1/6834 ,  C12Q1/686 ,  G01N33/54313 ,  B01L2400/0481 ,  Y10S435/81

    摘要： An element has been prepared which is useful for the detection of nucleic acids in various formats. The element has a sealable support on which is disposed a nucleic acid reagent composition. The composition is a mixture of a nucleic acid reagent composed of polymeric particles to which an oligonucleotide is covalently attached. The particles are prepared from a first polymer having a glass transition temperature of at least about 70.degree. C. and have an average diameter of from about 0.1 to about 3 micrometers. The reagent is adhered to the support using a water insoluble adhesive comprising a second polymer which has a glass transition temperature which is at least about 30.degree. C. less than the glass transition temperature of the first polymer. The adhesive is present in the composition at from about 1 to about 20 weight percent. This element provides high sensitivity and low background in hybridization and other nucleic acid assays.

    摘要翻译： 已经制备了可用于检测各种形式的核酸的元件。 元件具有可密封的载体，在其上设置核酸试剂组合物。 该组合物是由聚合物颗粒组成的核酸试剂与寡核苷酸共价连接的混合物。 颗粒由玻璃化转变温度至少为约70℃的第一聚合物制备，平均直径为约0.1至约3微米。 使用包含第二聚合物的水不溶性粘合剂将试剂粘附到载体上，所述第二聚合物的玻璃化转变温度比第一聚合物的玻璃化转变温度低至少约30℃。 粘合剂以约1至约20重量％存在于组合物中。 该元件在杂交和其他核酸测定中提供高灵敏度和低背景。