公开(公告)号：US20170281828A1

公开(公告)日：2017-10-05

申请号：US15513938

申请日：2015-09-24

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Kang ZHANG ,  Shaochen CHEN ,  Xin QU ,  Hong OUYANG

IPC: A61L27/38 ,  B33Y80/00 ,  C12N5/079 ,  B33Y10/00 ,  A61L27/52 ,  A61L27/48

CPC classification number: A61L27/3891 ,  A61L27/3808 ,  A61L27/3813 ,  A61L27/3834 ,  A61L27/48 ,  A61L27/50 ,  A61L27/52 ,  A61L2400/12 ,  A61L2430/16 ,  B33Y10/00 ,  B33Y80/00 ,  C12M33/00 ,  C12N5/0621 ,  C12N2513/00 ,  C12N2533/30 ,  C12N2533/54

Abstract: An artificial cornea is fabricated by separately culturing live stromal cells, live corneal endothelial cells (CECs) and live corneal epithelial cells (CEpCs), and 3D bioprinting separate stromal, CEC and CEpC layers to encapsulate the cells into separate hydrogel nanomeshes. The CEC layer is attached to a first side of the stromal layer and the CEpC layer to a second side of the stromal layer to define the artificial cornea.

公开(公告)号：US20210402678A1

公开(公告)日：2021-12-30

申请号：US17360891

申请日：2021-06-28

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Shangting You ,  Shaochen CHEN

IPC: B29C64/129 ,  B29C64/245 ,  B29C64/286 ,  B29C64/273 ,  B29C64/232 ,  B33Y10/00 ,  B33Y30/00

Abstract: A method and system for microscale 3D printing achieve high-fidelity fabrication through the control of the light exposure time. A single pulse of light is used to initiate polymerization of a pre-polymer solution to minimize scattering-induced resolution deterioration. The printed object is fabricated in a layer-by-layer construction where each layer is formed through exposure to a single light pulse.

公开(公告)号：US20250091286A1

公开(公告)日：2025-03-20

申请号：US18959285

申请日：2024-11-25

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： David Barnes BERRY ,  Shaochen CHEN

IPC: B29C64/106 ,  B29C64/209 ,  B29C64/264 ,  B29C64/321 ,  B33Y10/00 ,  B33Y40/00 ,  B33Y70/10 ,  B33Y80/00 ,  C09D11/04

Abstract: A device for in vivo 3D bioprinting includes an elongated hollow tube configured that can be inserted into a living body. A feed tube within the hollow tube conveys a liquid polymerizable biomaterial to an extrusion nozzle positioned at the target site. A light guide within the hollow tube conducts polymerizing light from a light source to polymerize the biomaterial that has been extruded at the target site. Arthroscopic procedures employing the device enable in vivo attachment of tissue to bone or other tissue, or replacement of loss tissue or bone volume.

公开(公告)号：US20170087766A1

公开(公告)日：2017-03-30

申请号：US15311135

申请日：2015-05-20

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Peter CHUNG ,  Xin QU ,  Aping ZHANG ,  Shaochen CHEN

IPC: B29C67/00 ,  B33Y10/00 ,  G01N33/50 ,  B33Y50/02 ,  B33Y70/00 ,  B33Y80/00 ,  B29C35/08 ,  B33Y30/00

CPC classification number: B33Y80/00 ,  B29C35/0805 ,  B29C35/0894 ,  B29C64/129 ,  B29C64/277 ,  B29C64/386 ,  B29C64/40 ,  B29C2035/0827 ,  B29C2035/0838 ,  B29K2995/0005 ,  B29K2995/0056 ,  B29L2031/752 ,  B29L2031/7532 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y50/02 ,  B33Y70/00 ,  G01N33/502 ,  G01N33/5058

Abstract: A system and method for 3D microfabrication projects light capable of initiating photopolymerization toward a spatial light modulator that modulates light responsive to digital masks corresponding to layers of the structure. Projection optics focus the modulated light onto an optical plane within a photopolymerizable material supported on a stage. A computer controller causes the spatial light modulator to project a sequence of images corresponding to the digital masks while coordinating movement of the stage to move a position of the optical plane within the photopolymerizable material to sequentially project each image of the sequence to generate the structure by progressively photopolymerizing the photopolymerizable material.

公开(公告)号：US20220322644A1

公开(公告)日：2022-10-13

申请号：US17639223

申请日：2020-08-28

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  UNIVERSITY OF CAMBRIDGE

Inventor： Shaochen CHEN ,  Dimitri DEHEYN ,  Shangting You ,  Daniel WANGPRASEURT ,  Silvia VIGNOLINI

IPC: A01K61/70 ,  B29C64/286 ,  B29C64/245 ,  B33Y10/00 ,  B33Y70/10 ,  B33Y80/00 ,  B29C64/129

Abstract: An artificial structure for promoting microalgae growth includes a 3D-printed structure formed by positioning a printing surface on a movable stage of a 3D bioprinter in contact with a bio-ink that includes a mixture of a pre-polymer material with one or more of cellulose-derived nanocrystals (CNC), and microalgae cells. By projecting modulated light onto the printing surface while moving the stage, the bio-ink is progressively polymerized to define layers of an artificial coral structure with microalgae cells disposed thereon, where the artificial coral structure is configured to scatter light within the structure.

公开(公告)号：US20170283766A1

公开(公告)日：2017-10-05

申请号：US15511429

申请日：2015-09-16

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Kolin C. HRIBAR ,  Shaochen CHEN ,  Darren FINLAY ,  Kristiina VUORI ,  Xuanyi MA

IPC: C12N5/00 ,  C12N5/09 ,  B33Y10/00 ,  C12M1/00 ,  B29C67/00 ,  C12N5/074 ,  C12M1/32

CPC classification number: C12N5/0062 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0858 ,  B01L2300/0893 ,  B29C64/124 ,  B29C64/129 ,  B29C64/135 ,  B33Y10/00 ,  C12M23/12 ,  C12M23/22 ,  C12M33/00 ,  C12N5/0693 ,  C12N5/0696 ,  C12N2513/00 ,  C12N2533/30

Abstract: Using 3D printing, a microwell is formed by providing a plurality of masks, each mask representing a cross-section of a layer of the concave structure. Progressive movement of a projection plane exposes a pre-polymer solution to polymerizing radiation modulated by the masks to define the layers of the microwell, where each layer is exposed for a non-equal exposure period as determined by a non-linear factor. In a preferred embodiment, a first portion of the masks are base layer masks, which are exposed for a longer period than subsequent exposure periods. Shapes of the microwells, which may include circular, square, annular, or other geometric shapes, and their depths, are selected to promote aggregation behavior in the target cells, which may include tumor cells and stem cells.

公开(公告)号：US20160298087A1

公开(公告)日：2016-10-13

申请号：US14895912

申请日：2014-06-04

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Xin QU ,  Maling GOU ,  Wei ZHU ,  Shaochen CHEN

IPC: C12N5/071

CPC classification number: C12N5/0671 ,  C12N2506/45 ,  C12N2533/30 ,  C12N2533/54 ,  C12N2533/80

Abstract: A liver-mimetic device and method include a 3D polymer scaffold having a matrix of liver-like lobules with hepatic-functioning particles encapsulated within the lobules. In some embodiments, each liver-like lobule is hexagonal in structure and the matrix is in a honeycomb arrangement. In some embodiments, the hepatic-functioning particles are hepatic progenitor cells. In other embodiments, the hepatic-functioning particles are polymer nanoparticles adapted to capture pore-forming toxins.

Abstract translation: 肝模拟装置和方法包括具有包封在小叶内的肝功能颗粒的肝样小叶的基质的3D聚合物支架。 在一些实施方案中，每个肝样小叶的结构是六边形的，并且基质是蜂窝状的。 在一些实施方案中，肝功能颗粒是肝祖细胞。 在其它实施方案中，肝功能颗粒是适于捕获成孔毒素的聚合物纳米颗粒。