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公开(公告)号：US06508411B1
公开(公告)日：2003-01-21
申请号：US09701552
申请日：2001-01-08
申请人： Takao Ohnishi , Iwao Ohwada , Toshikazu Hirota
发明人： Takao Ohnishi , Iwao Ohwada , Toshikazu Hirota
IPC分类号： B05B1704
CPC分类号： B41J2/04541 , B41J2/04581 , B41J2/04588 , B41J2/14233 , B41J2/14274 , F04B43/0081 , F04B43/046
摘要： A liquid-drop spraying device is provided which can perform a stable liquid discharge without producing air bubbles in the liquid of the pressurized room as well as the amount of liquid supply per unit time is increased. The liquid can be flowed into the pressurized room uniformly and without entrainment of bubbles from the nozzle side by making an initial discharge (or charge) time constant larger than a subsequent discharge (or charge) time constant, which in turn allows liquid in the pressurized room to be replaced by slow suction followed by fast suction.
摘要翻译： 提供一种液滴喷射装置，其能够在不会在加压室的液体中产生气泡的情况下进行稳定的液体排出，并且每单位时间的液体供应量增加。 通过使初始放电（或充电）时间常数大于随后的放电（或充电）时间常数，液体可以均匀地流入加压室并且不会从喷嘴侧夹带气泡，这又导致加压的液体 房间被缓慢的吸力替代，然后快速吸入。
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公开(公告)号：US06702196B2
公开(公告)日：2004-03-09
申请号：US10290034
申请日：2002-11-07
申请人： Takao Ohnishi , Iwao Ohwada , Toshikazu Hirota
发明人： Takao Ohnishi , Iwao Ohwada , Toshikazu Hirota
IPC分类号： B05B304
CPC分类号： B41J2/04541 , B41J2/04581
摘要： A circuit for driving a liquid drop spraying apparatus capable of smoothly supplying liquid to a pressurizing chamber is provided. A coil is provided in series with respect to a resistor that determines the charge characteristics of a charge circuit of a piezoelectric/electrostrictive element, thereby enabling gentle start characteristics when a charge voltage rises. In addition, discharge circuits are provided at two stages. A constant of the second discharging circuit is set to be smaller than a constant of the first discharging circuit. Coils are provided in series with resistors that determine their discharge characteristics. In this manner, even in the case where a large amount of liquid is sprayed, liquid is smoothly supplied to the pressurizing chamber.
摘要翻译： 提供一种用于驱动能够平稳地将液体供应到加压室的液滴喷射装置的电路。 线圈相对于确定压电/电致伸缩元件的充电电路的充电特性的电阻器被串联提供，从而当充电电压升高时能实现平稳的启动特性。 此外，放电电路分两级设置。 第二放电电路的常数设定为小于第一放电电路的常数。 线圈与确定其放电特性的电阻串联提供。 以这种方式，即使在喷射大量液体的情况下，液体平稳地供给到加压室。
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公开(公告)号：US07667194B2
公开(公告)日：2010-02-23
申请号：US11566351
申请日：2006-12-04
申请人： Motoo Noritake , Takao Ohnishi , Toshikazu Hirota
发明人： Motoo Noritake , Takao Ohnishi , Toshikazu Hirota
CPC分类号： B01J19/0046 , B01J2219/00378 , B01J2219/00529 , B01J2219/00533 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00628 , B01J2219/00637 , B01J2219/00659 , B01J2219/00662 , B01J2219/00677 , B01J2219/00693 , B01J2219/00722 , B01J2219/00725 , B01J2219/0074 , B01L3/0268 , B01L2200/143 , B01L2400/0439 , C40B40/06 , C40B40/10 , C40B40/14 , C40B60/14 , G01N2035/1041
摘要： A method of producing a microarray including: (A) ejecting a liquid sample from an outlet onto an inspection carrier to form inspection spots, inspecting the resultant inspection spots for their quality to determine whether the inspected spots are defective or successful, and detecting a defective discharge unit, if any; (B) making the detected defective discharge unit stop discharging the liquid sample to prevent formation of the defective sample spot; (C) forming successful sample spots on a carrier using successful discharge units to provide a successful microarray on which the successful spots are aligned in a predetermined pattern on the carrier; and (D) forming a successful spot to be formed originally on the successful microarray at the position of the defective spot where no spot is formed in step (B).
摘要翻译： 一种生产微阵列的方法，包括：（A）将液体样品从出口喷射到检查载体上以形成检查点，检查所得检查点的质量，以确定检查点是否有缺陷或成功，并检测缺陷 排放单位（如有）; （B）使检测到的有缺陷的排出单元停止排出液体样品以防止形成有缺陷的样品点; （C）使用成功的放电单元在载体上形成成功的样品点，以提供成功的斑点在载体上以预定图案对准的成功的微阵列; （D）在步骤（B）中在不形成斑点的缺陷点的位置上成功形成成功的微阵列的成功光斑。
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公开(公告)号：US06845759B2
公开(公告)日：2005-01-25
申请号：US10293242
申请日：2002-11-13
申请人： Takao Ohnishi , Toshikazu Hirota
发明人： Takao Ohnishi , Toshikazu Hirota
CPC分类号： F02M61/186 , F02M51/0603 , F02M51/061 , F02M61/047 , F02M61/166 , F02M61/1806 , F02M61/1853 , F02M69/041
摘要： A liquid fuel injection system includes an injection unit for atomizing liquid through application to the fuel vibration energy induced by the operation of piezoelectric/electrostrictive elements, and a discharge valve for discharging pressurized fuel into the injection unit. Only during a period of time when an intake valve of an internal combustion engine is closed, an electric controller supplies a drive voltage signal of a certain frequency to the piezoelectric/electrostrictive elements of the injection unit so as to operate the piezoelectric/electrostrictive elements, and a valve-opening drive signal to the discharge valve so as to discharge fuel into the injection unit from a fuel path of the discharge valve.
摘要翻译： 液体燃料喷射系统包括用于通过应用于由压电/电致伸缩元件的操作引起的燃料振动能量来雾化液体的喷射单元和用于将加压燃料排放到喷射单元中的排出阀。 仅在内燃机的进气门闭合的时间段内，电控制器将一定频率的驱动电压信号提供给喷射单元的压电/电致伸缩元件，以便操作压电/电致伸缩元件， 以及向所述排出阀的开阀驱动信号，以从所述排出阀的燃料通路将燃料喷射到所述喷射单元中。
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公开(公告)号：US06739520B2
公开(公告)日：2004-05-25
申请号：US10262521
申请日：2002-10-01
申请人： Takao Ohnishi , Toshikazu Hirota
发明人： Takao Ohnishi , Toshikazu Hirota
IPC分类号： B05B108
CPC分类号： F02M61/186 , F02D41/2096 , F02M51/0603 , F02M51/061 , F02M61/047 , F02M61/166 , F02M61/1806 , F02M61/1853 , F02M69/041
摘要： A liquid injection apparatus 10 comprises an injection device 15, and an electro-magnetic injection valve 14 for ejecting pressurized fuel into the injection device. An ejection hole in the electro-magnetic injection valve is connected to a hollow cylindrical hermetically sealed space, which in turn is connected to a liquid ejection nozzle, through a liquid filling port, a liquid supply passage, and a chamber which are included in the injection device. By means of allowing the electro-magnetic injection valve to eject the pressurized fuel at an angle of inclination relative to the center axis of the hollow cylindrical hermetically sealed space, flows of fuel are produced across a wide area in the hermetically sealed space so that large-sized air bubbles are prevented from being formed in the hermetically sealed space. The injection device adds to the liquid an oscillation energy based on a change of volume of the chamber caused by the piezoelectric/electrostrictive element, for atomizing the liquid to be injected.
摘要翻译： 液体注射装置10包括注射装置15和用于将加压燃料喷射到注射装置中的电磁注射阀14。 电磁注入阀中的喷射孔连接到中空圆柱形密封空间，该空心圆筒形密封空间又通过液体注入口，液体供应通道和腔室连接到液体喷射嘴， 注射装置。 通过允许电磁注入阀以相对于中空圆柱形密封空间的中心轴线倾斜的角度喷射加压燃料，在气密密封空间中的广泛区域上产生燃料流，使得大的 防止在密封空间中形成尺寸的气泡。 注射装置基于由压电/电致伸缩元件引起的室的体积变化而向液体添加振动能，用于雾化待注射的液体。
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公开(公告)号：US06752326B2
公开(公告)日：2004-06-22
申请号：US09884457
申请日：2001-06-19
申请人： Toshikazu Hirota , Takao Ohnishi
发明人： Toshikazu Hirota , Takao Ohnishi
IPC分类号： B05B108
CPC分类号： F02M61/1846 , B05B17/0607 , F02M51/04 , F02M51/0603 , F02M57/021 , F02M57/027 , F02M61/166 , F02M61/168 , F02M61/18 , F02M61/1833 , F02M61/186 , F02M2200/90 , F02M2200/9007 , F02M2200/9015 , F02M2200/9038 , F02M2200/9046
摘要： The liquid droplet ejection apparatus includes a liquid supply path, a plurality of mutually independent pressurizing chambers, a plurality of liquid introduction bores for establishing communication between the corresponding pressurizing chambers and the liquid supply path, and a plurality of ejection nozzles for establishing communication between the corresponding pressurizing chambers and the exterior of the liquid droplet ejection apparatus. An ejection bore formed at the end portion of the ejection nozzle has a hollow cylindrical form and the inside diameter thereof increases toward an ejection opening. When a potential difference is applied between two electrodes of a piezoelectric/electrostrictive element, a ceramic sheet forming the upper wall of the pressurizing chamber deforms to thereby cause a change of the volume of the pressurizing chamber. Thus, liquid pressure within the pressurizing chamber increases to thereby cause simultaneous ejection of a plurality of liquid droplets from the ejection opening.
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公开(公告)号：US06465190B1
公开(公告)日：2002-10-15
申请号：US09694135
申请日：2000-10-23
IPC分类号： C12Q168
CPC分类号： G01N35/00029 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00619 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01L3/0268 , B01L3/5088 , B01L7/52 , B01L2300/10 , B01L2400/0442 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N2035/00158 , G01N2035/1039 , C12Q2565/507
摘要： Disclosed is a method comprising a pretreatment step of forming a poly-L-lysine layer on a surface of a base plate, a sample preparation step of preparing a sample containing a DNA fragment, a dilution step of diluting the concentration of the obtained sample, and a supply step of supplying a diluted sample solution onto the base plate to produce a DNA chip. The sample preparation step includes an amplification step of PCR-amplifying the DNA fragment to prepare a PCR product, a powder formation step of drying the obtained PCR product to form DNA powder, and a mixing step of dissolving the obtained DNA powder in a buffer solution.
摘要翻译： 公开了一种方法，其包括在基板的表面上形成聚-L-赖氨酸层的预处理步骤，制备含有DNA片段的样品的样品制备步骤，稀释所得样品的浓度的稀释步骤， 以及将稀释的样品溶液供应到基板上以产生DNA芯片的供给步骤。 样品制备步骤包括PCR扩增DNA片段以制备PCR产物的扩增步骤，将获得的PCR产物干燥以形成DNA粉末的粉末形成步骤和将获得的DNA粉末溶解在缓冲溶液中的混合步骤 。
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公开(公告)号：US07588322B2
公开(公告)日：2009-09-15
申请号：US11669608
申请日：2007-01-31
IPC分类号： B41J2/045
CPC分类号： B41J2/14209 , B41J2002/14217 , B41J2202/11
摘要： A liquid droplet discharging piezoelectric device 1 provided with a cavity member 11 with a built-in cavity 3; an introduction member 13 having introduction channel 5 connecting with the cavity 3; and a nozzle member 12 having nozzle channel 4 connecting with the cavity 3 on a side opposite to the channel 5. This liquid droplet discharging piezoelectric device 1 is provided with an introduction port 6, attached to the introduction member 13, capable of introducing a liquid into the cavity 3 via the introduction channel 5, and a discharge port 7, attached to the nozzle member 12, capable of discharging as droplets a liquid filled in the cavity 3 via the nozzle channel 40. Even in a case where an amount of liquid droplets is of a nanoliter (nl) order, excellent stability and reproducibility are attained, and the unit can stably be operated when attached to an apparatus.
摘要翻译： 具有内置空腔3的空腔构件11的液滴排出压电装置1; 引入构件13，其具有与空腔3连接的引入通道5; 以及喷嘴构件12，其具有在与通道5相反的一侧与空腔3连接的喷嘴通道4.该液滴排出压电装置1设置有引入口6，引入口6安装在导入构件13上，能够引入液体 通过引入通道5进入空腔3和附接到喷嘴构件12的排出口7，其能够通过喷嘴通道40作为液滴喷射填充在空腔3中的液体。即使在液体量 液滴具有纳升级（nl）级，达到优异的稳定性和再现性，并且当装置安装时该装置可以稳定地操作。
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公开(公告)号：US07160512B2
公开(公告)日：2007-01-09
申请号：US10135200
申请日：2002-04-30
申请人： Toshikazu Hirota , Takao Ohnishi , Hiroyuki Tsuji
发明人： Toshikazu Hirota , Takao Ohnishi , Hiroyuki Tsuji
IPC分类号： B01L3/02
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00378 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00659 , B01J2219/00677 , B01J2219/00689 , B01J2219/00691 , B01J2219/00693 , B01J2219/00695 , B01J2219/00702 , B01J2219/00722 , B01L3/0241 , C40B40/06 , C40B60/14 , G01N35/109 , Y10T436/2575
摘要： A biochip manufacturing method can densely align spots of a plurality of samples in predetermined locations on a substrate using a discharge head equipped with one or more discharge modules composed of one or more discharge units, and a first moving table on which one or more trays with one or more substrates fixed thereon are detachably mounted. According to the biochip manufacturing method, the high precision in the operation for densely aligning and fixing droplets with a minute volume on a predetermined substrate (micro spot-forming operation) can be attained, and the time required for the micro spot-forming operation can be shortened as well.
摘要翻译： 生物芯片制造方法可以使用配备有由一个或多个排出单元组成的一个或多个排出模块的排出头以及第一移动工作台，将一个或多个具有 固定在其上的一个或多个基板可拆卸地安装。 根据生物芯片的制造方法，可以实现在规定的基板上微小对准和固定微小的液滴的操作的高精度（微点形成操作），并且微点成形操作所需的时间可以 也缩短了。
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公开(公告)号：US06875402B2
公开(公告)日：2005-04-05
申请号：US09977567
申请日：2001-10-15
申请人： Toshikazu Hirota , Takao Ohnishi
发明人： Toshikazu Hirota , Takao Ohnishi
CPC分类号： B01L3/0268 , B01J19/0046 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00659 , B01J2219/00722 , C40B40/06 , C40B60/14 , G01N2035/1041 , Y10S73/04 , Y10T436/2575
摘要： A micropipette usable for producing a biochip inclusive of DNA micro arrays capable of arraying and fixing droplets of a micro-volume on a substrate in a high density includes a main body, at least one cavity for storing a sample, at least one ejection port, a piezoelectic/electrostrictive element mounted on the outer surface of the main body, and at least one sample inlet port for supplying sample from the outside. A sample that is temporarily stored in the cavity is discharged from at least one ejection port, by virtue of the movement of the piezoelectric/electrostrictive element, to outside of the micropipette via a through hole in a nozzle portion formed in the pipette main body. The nozzle portion through hole has three or more projection radially protruding from the center of the through hole and having a specific shape.
摘要翻译： 可用于生产包括能够以高密度排列和固定微量体积的液滴的DNA微阵列的生物芯片的微量移液器包括主体，至少一个用于存储样品的空腔，至少一个排出口， 安装在主体的外表面上的压电/电致伸缩元件和用于从外部供应样品的至少一个样品入口。 通过压电/电致伸缩元件的移动，临时存储在空腔中的样品通过形成在移液管主体中的喷嘴部分中的通孔从至少一个喷射口排出到微量移液管外部。 喷嘴部通孔具有从通孔的中心径向突出并具有特定形状的三个以上的突起。
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