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    Protease with low thermostability derived from Mucor pusillus
    5.
    发明授权
    Protease with low thermostability derived from Mucor pusillus 失效
    来自毛霉菌的热稳定性低的蛋白酶

    公开(公告)号：US5332668A

    公开(公告)日：1994-07-26

    申请号：US958222

    申请日：1992-10-08

    申请人： Takashi Yamashita ,  Susumu Higashi ,  Toshihiko Higashi ,  Haruo Machida ,  Shinjiro Iwasaki ,  Teruhiko Beppu

    发明人： Takashi Yamashita ,  Susumu Higashi ,  Toshihiko Higashi ,  Haruo Machida ,  Shinjiro Iwasaki ,  Teruhiko Beppu

    IPC分类号： C12N9/58 ,  C12N15/09 ,  C12N15/57 ,  C12N15/81 ,  C12R1/865 ,  C12N9/50

    CPC分类号： C12N9/58 ,  C12N15/81 ,  Y10S435/931

    摘要： A mutant fungus strain which produces a protease with low thermostability and low productivity was made from protease producing fungi and the gene coding for mutant enzyme is isolated from the mutant strain. A promoter which can function in yeast is ligated to the gene and inserted into a plasmid replicable in yeast, and the resulting plasmid is introduced into yeast. The yeast is cultured, thereby producing the mutant enzyme. Furthermore, a gene expressing an enzyme with a far lower thermostability is prepared by site-directed mutagenesis, which is introduced in yeast, thereby producing an enzyme with more distinctively reduced thermostability.

    摘要翻译： 由产生蛋白酶的真菌产生具有低热稳定性和低生产率的蛋白酶的突变真菌菌株，并从突变菌株中分离编码突变酶的基因。 将可在酵母中发挥作用的启动子与基因连接并插入到酵母中可复制的质粒中，将得到的质粒导入酵母中。 培养酵母，从而产生突变酶。 此外，通过在酵母中导入的定点诱变来制备表达具有远低于热稳定性的酶的基因，由此制备具有更显着降低的热稳定性的酶。

    Thermally stable tryptophanase, process for producing the same, and thermally stable tryptophanase-producing microorganism
    7.
    发明授权
    Thermally stable tryptophanase, process for producing the same, and thermally stable tryptophanase-producing microorganism 失效
    热稳定的色氨酸酶，其制备方法和热稳定的产生色氨酸酶的微生物

    公开(公告)号：US4761374A

    公开(公告)日：1988-08-02

    申请号：US871315

    申请日：1986-06-06

    申请人： Teruhiko Beppu ,  Seibun Suzuki

    发明人： Teruhiko Beppu ,  Seibun Suzuki

    IPC分类号： C12N9/88 ,  C12P13/22 ,  C12P39/00 ,  C12R1/01 ,  C12R1/07 ,  C12N1/20

    CPC分类号： C12R1/07 ,  C12N9/88 ,  C12P13/227 ,  C12P39/00 ,  C12R1/01 ,  Y10S435/822 ,  Y10S435/832

    摘要： A thermally stable tryptophanase having (1) an optimum temperature for activity at a pH of 8.0 of about 70.degree. C., and (2) such thermal stability that it is not thermally deactivated when maintained at temperatures up to about 65.degree. C. and a pH of 8.0 for 40 minutes. The thermally stable tryptophanase can be produced by cultivating in a tryptophan-containing culture medium a thermally stable tryptophanase-producing bacterium which does not grow alone in said medium but grows there in the presence of Bacillus sp. strain S, and obtaining the resulting thermally stable tryptophanase from the culture broth. The thermally stable tryptophanase-producing microorganism for use in the above process is a novel organism.

    摘要翻译： 一种热稳定的色氨酸酶，其具有（1）在约8.0℃的pH为8.0时的活性的最适温度，和（2）这样的热稳定性，其在高至约65℃的温度下保持不热失活， pH为8.0，持续40分钟。 热稳定色氨酸酶可以通过在含色氨酸的培养基中培养产生热稳定的产生色氨酸的热稳定的色氨酸酶细菌来生产，所述细菌在所述培养基中不单独生长，而在芽孢杆菌存在下在那里生长。 菌株S，从培养液中得到热稳定的色氨酸酶。 用于上述方法的热稳定的产生色氨酸酶的微生物是新的生物体。