公开(公告)号：US06395472B1

公开(公告)日：2002-05-28

申请号：US09245248

申请日：1999-02-05

申请人： Thomas P. Leary ,  James Erker ,  Michelle Chalmers ,  John Simons ,  Larry Birkenmeyer ,  Scott Muerhoff ,  Tami Pilot-Matias ,  Suresh Desai ,  Isa Mushahwar

发明人： Thomas P. Leary ,  James Erker ,  Michelle Chalmers ,  John Simons ,  Larry Birkenmeyer ,  Scott Muerhoff ,  Tami Pilot-Matias ,  Suresh Desai ,  Isa Mushahwar

IPC分类号： C12Q170

CPC分类号： C12Q1/701 ,  Y10S977/804

摘要： The present invention relates to nucleic oligomer primers or probes useful for detection of TTV in test samples. Also provided are assays which utilize these primers and probes, as well as test kits which contain these oligomer primers and/or probes. In addition, the present invention encompasses the use of TTV nucleotide sequences as nucleic acid vectors and as markers for determining transmission between individuals as well as the route thereof. Additionally, the present invention encompasses a method of detecting TTV infection prior to xenotransplatation of a tissue or organ.

摘要翻译： 本发明涉及用于在测试样品中检测TTV的核酸低聚物引物或探针。 还提供了利用这些引物和探针的测定法，以及含有这些寡聚体引物和/或探针的测试试剂盒。 此外，本发明包括TTV核苷酸序列作为核酸载体的用途以及用于确定个体间传播的标记及其途径。 此外，本发明包括在组织或器官的异种移植之前检测TTV感染的方法。

公开(公告)号：US5807670A

公开(公告)日：1998-09-15

申请号：US580038

申请日：1995-12-21

申请人： Anthony Scott Muerhoff ,  John N. Simons ,  Thomas P. Leary ,  Larry Birkenmeyer ,  James C. Erker ,  Michelle Chalmers ,  George J. Dawson ,  Suresh M. Desai ,  Isa K. Mushahwar

发明人： Anthony Scott Muerhoff ,  John N. Simons ,  Thomas P. Leary ,  Larry Birkenmeyer ,  James C. Erker ,  Michelle Chalmers ,  George J. Dawson ,  Suresh M. Desai ,  Isa K. Mushahwar

IPC分类号： G01N33/566 ,  C07K14/18 ,  C12N15/09 ,  C12N15/113 ,  C12N15/86 ,  C12Q1/70 ,  G01N33/576

CPC分类号： C12N15/1131 ,  C07K14/005 ,  C12N15/86 ,  C12Q1/707 ,  C07K2319/00 ,  C12N2310/315 ,  C12N2310/3181 ,  C12N2310/33 ,  C12N2770/24222 ,  C12N2840/203 ,  C12Q1/6813

摘要： HGBV-C oligonucleotides from the 5' end of HGBV-C useful for the detection and genotyping of HGBV-C isolates. Also provided are assays which utilize these oligonucleotides.

摘要翻译： 来自HGBV-C的5'端的HGBV-C寡核苷酸可用于HGBV-C分离物的检测和基因分型。 还提供了利用这些寡核苷酸的测定法。

公开(公告)号：US07101683B2

公开(公告)日：2006-09-05

申请号：US09891983

申请日：2001-06-26

申请人： Dinesh O. Shah ,  George J. Dawson ,  Anthony Scott Muerhoff ,  Xingli Jiang ,  Robin A. Gutierrez ,  Thomas P. Leary ,  Suresh Desai ,  James L. Stewart

发明人： Dinesh O. Shah ,  George J. Dawson ,  Anthony Scott Muerhoff ,  Xingli Jiang ,  Robin A. Gutierrez ,  Thomas P. Leary ,  Suresh Desai ,  James L. Stewart

IPC分类号： C11Q1/00 ,  C12Q1/70 ,  G01N33/53

CPC分类号： C07K14/005 ,  C12N2770/24222 ,  G01N33/5767 ,  G01N2469/10 ,  G01N2469/20

摘要： The subject invention relates to methods for the simultaneous detection of Hepatitis C Virus (HCV) antigens as well as antibodies produced in response to HCV antigens. Furthermore, the subject invention allows one to detect antigens in the early, acute stage of infection, even prior to the development of antibodies, thereby allowing for early detection of infected blood and blood products, thus improving the safety of the blood supply.

公开(公告)号：US06727092B2

公开(公告)日：2004-04-27

申请号：US10173480

申请日：2002-06-17

申请人： Dinesh Shah ,  George Dawson ,  A. Scott Muerhoff ,  Thomas P. Leary ,  Robin A. Guetierrez ,  Lily Jiang ,  Suresh Desai ,  James L. Stewart

发明人： Dinesh Shah ,  George Dawson ,  A. Scott Muerhoff ,  Thomas P. Leary ,  Robin A. Guetierrez ,  Lily Jiang ,  Suresh Desai ,  James L. Stewart

IPC分类号： C12N1574

CPC分类号： C07K14/005 ,  C12N2770/24222 ,  G01N33/5767 ,  G01N2469/10 ,  G01N2469/20

摘要： The subject invention relates to methods for the simultaneous detection of Hepatitis C Virus (HCV) antigens as well as antibodies produced in response to HCV antigens. Furthermore, the subject invention allows one to detect antigens in the early, acute stage of infection, even prior to the development of antibodies, thereby allowing for early detection of infected blood and blood products, thus improving the safety of the blood supply.

摘要翻译： 本发明涉及同时检测丙型肝炎病毒（HCV）抗原以及响应HCV抗原产生的抗体的方法。 此外，本发明允许在感染的早期，急性期中检测抗原，甚至在抗体发生之前，从而允许早期检测感染的血液和血液制品，从而提高血液供应的安全性。

公开(公告)号：US06855809B2

公开(公告)日：2005-02-15

申请号：US10753910

申请日：2004-01-07

申请人： Dinesh O. Shah ,  George J. Dawson ,  A. Scott Muerhoff ,  Lily Jiang ,  Robin A. Gutierrez ,  Thomas P. Leary ,  Suresh Desai ,  James L. Stewart

发明人： Dinesh O. Shah ,  George J. Dawson ,  A. Scott Muerhoff ,  Lily Jiang ,  Robin A. Gutierrez ,  Thomas P. Leary ,  Suresh Desai ,  James L. Stewart

IPC分类号： G01N33/543 ,  C07K14/18 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12P21/04 ,  G01N21/76 ,  G01N33/576 ,  C07K14/00 ,  A61K39/00 ,  C07H21/00 ,  C07K14/08 ,  C12N15/62

CPC分类号： C07K14/005 ,  C12N2770/24222 ,  G01N33/5767 ,  G01N2469/10 ,  G01N2469/20

摘要： The subject invention relates to methods for the simultaneous detection of Hepatitis C Virus (HCV) antigens as well as antibodies produced in response to HCV antigens. Furthermore, the subject invention allows one to detect antigens in the early, acute stage of infection, even prior to the development of antibodies, thereby allowing for early detection of infected blood and blood products, thus improving the safety of the blood supply.

摘要翻译： 本发明涉及同时检测丙型肝炎病毒（HCV）抗原以及响应HCV抗原产生的抗体的方法。 此外，本发明允许在感染的早期，急性期中检测抗原，甚至在抗体发生之前，从而允许早期检测感染的血液和血液制品，从而提高血液供应的安全性。

公开(公告)号：US06455255B1

公开(公告)日：2002-09-24

申请号：US09631349

申请日：2000-08-02

申请人： Larry G. Birkenmeyer ,  Thomas P. Leary ,  A. Scott Muerhoff ,  Suresh M. Desai ,  Isa K. Mushahwar

发明人： Larry G. Birkenmeyer ,  Thomas P. Leary ,  A. Scott Muerhoff ,  Suresh M. Desai ,  Isa K. Mushahwar

IPC分类号： C12Q168

CPC分类号： C12Q1/6809 ,  C12Q2525/131 ,  C12Q2521/501

摘要： The subject invention relates to improvements in nucleic acid isolation, and more particularly, relates to modifications to the subtractive hybridization method and to reagents such as oligonucleotides that are useful when performing the method.

摘要翻译： 本发明涉及核酸分离的改进，更具体地涉及减法杂交方法的修饰以及在进行该方法时有用的诸如寡核苷酸的试剂。

公开(公告)号：US20120202295A1

公开(公告)日：2012-08-09

申请号：US13168373

申请日：2011-06-24

申请人： Thomas P. Leary ,  Robin A. Gutierrez ,  A. Scott Muerhoff ,  George J. Dawson ,  Suresh M. Desai ,  Dinesh Shah

发明人： Thomas P. Leary ,  Robin A. Gutierrez ,  A. Scott Muerhoff ,  George J. Dawson ,  Suresh M. Desai ,  Dinesh Shah

IPC分类号： G01N33/576 ,  C12N1/21 ,  C12N15/70 ,  C07K14/18 ,  C12N15/51

CPC分类号： G01N33/5767 ,  C07K14/005 ,  C12N2770/24222 ,  G01N2333/186 ,  G01N2469/20

摘要： A polypeptide comprising the contiguous amino acids 1-198 of SEQ ID NO: 2; a polypeptide, which comprises a contiguous amino acid sequence that is at least about 95% identical to the contiguous amino acids 1-198 of SEQ ID NO: 2, an epitope that is immunoreactive with an antibody that specifically binds to the core protein of hepatitis C virus (HCV), and an epitope that is immunoreactive with an antibody that specifically binds to the NS4 region of HCV; a nucleic acid encoding such a polypeptide; a host cell comprising such a nucleic acid; an immunodiagnostic reagent comprising such a polypeptide; a kit comprising such an immunodiagnostic reagent; and a method of determining the presence, amount, or concentration of anti-HCV antibodies in a test sample.

摘要翻译： 包含SEQ ID NO：2的连续氨基酸1-198的多肽; 一种多肽，其包含与SEQ ID NO：2的连续氨基酸1-198至少约95％相同的连续氨基酸序列，与特异性结合肝炎核心蛋白的抗体具有免疫反应性的表位 C病毒（HCV）和与特异性结合HCV的NS4区域的抗体免疫反应的表位; 编码这种多肽的核酸; 包含这种核酸的宿主细胞; 包含这种多肽的免疫诊断试剂; 包含这种免疫诊断试剂的试剂盒; 以及确定测试样品中抗HCV抗体的存在，量或浓度的方法。

公开(公告)号：US06558898B1

公开(公告)日：2003-05-06

申请号：US08488446

申请日：1995-06-07

申请人： John N. Simons ,  Tami J. Pilot-Matias ,  George J. Dawson ,  George G. Schlauder ,  Suresh M. Desai ,  Thomas P. Leary ,  Anthony Scott Muerhoff ,  James Carl Erker ,  Sheri L. Buijk ,  Isa K. Mushahwar

发明人： John N. Simons ,  Tami J. Pilot-Matias ,  George J. Dawson ,  George G. Schlauder ,  Suresh M. Desai ,  Thomas P. Leary ,  Anthony Scott Muerhoff ,  James Carl Erker ,  Sheri L. Buijk ,  Isa K. Mushahwar

IPC分类号： C12Q170

CPC分类号： C07K14/005 ,  A61K39/00 ,  C07K16/109 ,  C07K2319/00 ,  C12N2770/24222 ,  C12Q1/707 ,  G01N33/576 ,  Y10S435/81 ,  Y10S530/826 ,  Y10S977/906 ,  Y10S977/915

摘要： Hepatitis GB Virus (HGBV) nucleic acid and amino acid sequences useful for a variety of diagnostic and therapeutic applications, kits for using the HGBV nucleic acid or amino acid sequences, HGBV immunogenic particles, and antibodies which specifically bind to HGBV. Also provided are methods for producing antibodies, polyclonal or monoclonal, from the HGBV nucleic acid or amino acid sequences.

公开(公告)号：US06451578B1

公开(公告)日：2002-09-17

申请号：US08469260

申请日：1995-06-06

申请人： John N. Simons ,  Tami J. Pilot-Matias ,  George J. Dawson ,  George G. Schlauder ,  Suresh M. Desai ,  Thomas P. Leary ,  Anthony Scott Muerhoff ,  James Carl Erker ,  Sheri L. Buijk ,  Isa K. Mushahwar

发明人： John N. Simons ,  Tami J. Pilot-Matias ,  George J. Dawson ,  George G. Schlauder ,  Suresh M. Desai ,  Thomas P. Leary ,  Anthony Scott Muerhoff ,  James Carl Erker ,  Sheri L. Buijk ,  Isa K. Mushahwar

IPC分类号： C12N700

CPC分类号： C07K14/005 ,  A61K39/00 ,  C07K16/109 ,  C07K2319/00 ,  C12N2770/24222 ,  C12Q1/707 ,  G01N33/576 ,  Y10S436/82 ,  Y10S977/803

摘要： Hepatitis GB Virus (HGBV) nucleic acid and amino acid sequences useful for a variety of diagnostic and therapeutic applications, kits for using the HGBV nucleic acid or amino acid sequences, HGBV immunogenic particles, and antibodies which specifically bind to HGBV. Also provided are methods for producing antibodies, polyclonal or monoclonal, from the HGBV nucleic acid or amino acid sequences.

摘要翻译： 用于各种诊断和治疗应用的乙型肝炎病毒（HGBV）核酸和氨基酸序列，用于使用HGBV核酸或氨基酸序列的试剂盒，HGBV免疫原性颗粒和特异性结合HGBV的抗体。 还提供了从HGBV核酸或氨基酸序列产生抗体，多克隆或单克隆抗体的方法。

公开(公告)号：US6156495A

公开(公告)日：2000-12-05

申请号：US629463

申请日：1996-04-19

申请人： Tami J. Pilot-Matias ,  Thomas P. Leary ,  John N. Simons ,  Robert J. Carrick ,  Teresa K. Surowy ,  Suresh M. Desai ,  George J. Dawson ,  Anthony Scott Muerhoff ,  Isa K. Mushahwar

发明人： Tami J. Pilot-Matias ,  Thomas P. Leary ,  John N. Simons ,  Robert J. Carrick ,  Teresa K. Surowy ,  Suresh M. Desai ,  George J. Dawson ,  Anthony Scott Muerhoff ,  Isa K. Mushahwar

IPC分类号： G01N33/53 ,  A61K38/00 ,  A61K39/00 ,  A61K39/29 ,  A61P31/12 ,  C07H21/02 ,  C07H21/04 ,  C07K14/02 ,  C07K14/08 ,  C07K14/10 ,  C07K14/18 ,  C07K16/00 ,  C07K16/10 ,  C07K19/00 ,  C12N15/02 ,  C12N15/09 ,  C12N15/40 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/70 ,  C12R1/91 ,  G01N33/536 ,  G01N33/569 ,  G01N33/576 ,  G01N33/577 ,  C12N15/00

CPC分类号： C07K14/005 ,  C12Q1/707 ,  G01N33/576 ,  G01N33/5767 ,  A61K38/00 ,  A61K39/00 ,  C07K2319/00 ,  C12N2770/24222 ,  C12N2770/36022 ,  Y10S530/826

摘要： Recombinantly produced hepatitis GB Virus (HGBV) amino acid sequences useful for a variety of diagnostic and therapeutic applications, kits for using the HGBV amino acid sequences and antibodies which specifically bind to HGBV. Also provided are methods for producing antibodies, polyclonal or monoclonal, from the HGBV recombinantly produced amino acid sequences.

摘要翻译： 用于各种诊断和治疗应用的重组产生的乙型肝炎病毒（HGBV）氨基酸序列，使用特异性结合HGBV的HGBV氨基酸序列和抗体的试剂盒。 还提供了从HGBV重组产生的氨基酸序列产生抗体，多克隆或单克隆抗体的方法。