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    System and method for manipulating magnetically responsive particles fluid samples to collect DNA or RNA from a sample
    1.
    发明授权
    System and method for manipulating magnetically responsive particles fluid samples to collect DNA or RNA from a sample 有权
    用于操作磁响应颗粒流体样品以从样品中收集DNA或RNA的系统和方法

    公开(公告)号：US06672458B2

    公开(公告)日：2004-01-06

    申请号：US09858889

    申请日：2001-05-17

    申请人： Timothy Roy Hansen ,  Bradley Scott Thomas ,  John Joseph Bianco ,  Matthew P. Collis

    发明人： Timothy Roy Hansen ,  Bradley Scott Thomas ,  John Joseph Bianco ,  Matthew P. Collis

    IPC分类号： G01N33487

    CPC分类号： B01L7/52 ,  B03C1/288 ,  B03C2201/26 ,  C12N15/1013 ,  G01N35/0098 ,  G01N35/0099 ,  G01N35/028 ,  G01N2035/103

    摘要： A system and method for manipulating magnetically responsive particles in a solution to separate nucleic acid molecules from cell components in a cell solution. The system and method employ a device capable of receiving a plurality of tubes, each of which contain respective sample and magnetically responsive particles. The device includes heating and cooling devices to facilitate a lysing step to release the nucleic acid molecules from the cells in the cell solution. The device further includes moveable magnets which can be moved proximate to and away from the tube to hold the magnetically responsive particles to which the nucleic acid molecules become bound, so that the molecule-bound particles can be separated from the remainder of the solution, and washed as appropriate. The system also employs an electromagnet which is capable of demagnetizing the particles to allow the particles to freely mix with solution, such as elution solutions which are used to unbind the molecules from the particles.

    摘要翻译： 用于操纵溶液中的磁响应颗粒的系统和方法，以将核酸分子与细胞溶液中的细胞组分分离。 该系统和方法采用能够接收多个管的装置，每个管包含相应的样品和磁响应颗粒。 该装置包括加热和冷却装置以促进裂解步骤以从细胞溶液中的细胞释放核酸分子。 该装置还包括可移动的磁体，其可以靠近和远离管移动以保持与核酸分子结合的磁响应颗粒，使得分子结合的颗粒可以与溶液的其余部分分离，以及 适当洗涤。 该系统还采用电磁体，其能够使颗粒退磁以允许颗粒自由地与溶液混合，例如用于使分子与颗粒分离的洗脱溶液。

    Method for reducing inhibitors of nucleic acid hybridization
    8.
    发明授权
    Method for reducing inhibitors of nucleic acid hybridization 失效
    降低核酸杂交抑制剂的方法

    公开(公告)号：US06210881B1

    公开(公告)日：2001-04-03

    申请号：US09010219

    申请日：1998-01-21

    申请人： Michael C. Little ,  Oscar J. Llorin ,  Matthew P. Collis

    发明人： Michael C. Little ,  Oscar J. Llorin ,  Matthew P. Collis

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6806 ,  C12Q1/6832 ,  C12Q2527/125

    摘要： The present invention relates to a method for reducing the amount of substances inhibitory to nucleic acid hybridization in samples. The method is practiced prior to release of target nucleic acid from cells of interest and involves contacting the sample with an agent which solubilizes the inhibitory substances and does not effectuate release of nucleic acids from cells in the sample, and then the cells from the agent.

    摘要翻译： 本发明涉及减少样品中核酸杂交抑制物质的量的方法。 该方法在从目标细胞释放靶核酸之前进行，并且包括将样品与增溶抑制物质的试剂接触，并且不会使样品中的细胞释放核酸，然后从试剂中释放细胞。