公开(公告)号：US10711318B2

公开(公告)日：2020-07-14

申请号：US16194923

申请日：2018-11-19

Applicant: University of Massachusetts

Inventor： Derek R. Lovley ,  Toshiyuki Ueki ,  Kelly N. Lovley

IPC: C12M1/42 ,  C12P5/00 ,  C12P7/40 ,  C12N9/10 ,  C12N9/04 ,  C12M1/00 ,  C12P7/06 ,  C12R1/01

Abstract: A genetically engineered strain of Geobacter sulfurreducens exhibits improved function as a cathode biofilm compared to the wild type strain. The genetically engineered Geobacter sulfurreducens strain is capable of using carbon dioxide as a carbon source and electrical current as an energy source for producing a carbonaceous chemical using a reverse tricarboxylic acid pathway.

公开(公告)号：US11982637B2

公开(公告)日：2024-05-14

申请号：US17302063

申请日：2021-04-22

Applicant: University of Massachusetts

Inventor： Jun Yao ,  Derek R. Lovley ,  Alexander Smith ,  Xiaomeng Liu

IPC: G01N27/12 ,  A61B5/08 ,  G01N33/00

CPC classification number: G01N27/127 ,  A61B5/082 ,  G01N27/121 ,  G01N33/0054 ,  A61B2562/0285

Abstract: A gas sensor includes a biomaterial comprising electrically-conductive protein nanowires and at least two electrodes. The at least two electrodes are in operative arrangement with the protein nanowires and configured to provide a signal indicative of a change in conductivity of the protein nanowires. The conductivity of the protein nanowires is responsive to a change in concentration of a gas exposed to the biomaterial, such as ammonia, or to a change in relative humidity.

公开(公告)号：US20210341406A1

公开(公告)日：2021-11-04

申请号：US17302063

申请日：2021-04-22

Applicant: University of Massachusetts

Inventor： Jun Yao ,  Derek R. Lovley ,  Alexander Smith ,  Xiaomeng Liu

IPC: G01N27/12 ,  A61B5/08 ,  G01N33/00

Abstract: A gas sensor includes a biomaterial comprising electrically-conductive protein nanowires and at least two electrodes. The at least two electrodes are in operative arrangement with the protein nanowires and configured to provide a signal indicative of a change in conductivity of the protein nanowires. The conductivity of the protein nanowires is responsive to a change in concentration of a gas exposed to the biomaterial, such as ammonia, or to a change in relative humidity.

公开(公告)号：US20180269510A1

公开(公告)日：2018-09-20

申请号：US15609036

申请日：2017-05-31

Applicant: University of Massachusetts

Inventor： Derek R. Lovley ,  Toshiyuki Ueki ,  Kelly P. Nevin

IPC: H01M8/16 ,  C12N1/20 ,  C12N15/72 ,  C12N15/63 ,  C12N15/52 ,  C12N15/01

CPC classification number: H01M8/16 ,  C12N1/20 ,  C12N15/01 ,  C12N15/52 ,  C12N15/635 ,  C12N15/72 ,  C12N15/74 ,  C12Q1/005 ,  C12Q1/02 ,  C12Y203/03001

Abstract: A biosensor that employs a bacterium in a biofilm present on one of two electrodes of an electrochemical cell. The bacterium is genetically modified by having deleted at least one native gene that encodes for the enzymatic transformation of a molecular moiety, and having substituted for the deleted native gene a different gene having a transcription factor that is under the control of an inducible promoter in conjunction with an inducer molecule. When the molecular moiety is present in a specimen of interest that is in contact with the biofilm, an electric current is generated in response to the presence of the inducer molecule. In the absence of the inducer molecule, no electricity is generated. The electric signal that is generated can be analyzed to determine the presence and the quantity of the inducer molecule in the specimen of interest.

公开(公告)号：US09856449B2

公开(公告)日：2018-01-02

申请号：US14860211

申请日：2015-09-21

Applicant: UNIVERSITY OF MASSACHUSETTS

Inventor： Derek R. Lovley ,  Kelly P. Nevin

IPC: C12M1/42 ,  C12M1/34 ,  C12M1/00 ,  C25B3/00 ,  C12P7/40 ,  C12P7/54 ,  C12P7/16 ,  C25B3/04 ,  H01M8/16

CPC classification number: C12M35/02 ,  C12M21/12 ,  C12M41/00 ,  C12P7/16 ,  C12P7/40 ,  C12P7/54 ,  C25B3/00 ,  C25B3/04 ,  H01M8/16 ,  Y02E50/10 ,  Y02E60/527 ,  Y02P20/133 ,  Y02P20/135 ,  Y02P70/56

Abstract: The invention provides systems and methods for generating organic compounds using carbon dioxide as a source of carbon and electrical current as an energy source. In one embodiment, a reaction cell is provided having a cathode electrode and an anode electrode that are connected to a source of electrical power, and which are separated by a permeable membrane. A biological film is provided on the cathode. The biological film comprises a bacterium that can accept electrons and that can convert carbon dioxide to a carbon-bearing compound and water in a cathode half-reaction. At the anode, water is decomposed to free molecular oxygen and solvated protons in an anode half-reaction. The half-reactions are driven by the application of electrical current from an external source. Compounds that have been produced include acetate, butanol, 2-oxobutyrate, propanol, ethanol, and formate.

公开(公告)号：US11823808B2

公开(公告)日：2023-11-21

申请号：US16575861

申请日：2019-09-19

Applicant: University of Massachusetts

Inventor： Derek R. Lovley ,  Todd Emrick ,  Yun-Lu Sun ,  Brian Montz

IPC: H01B1/12 ,  H01B3/44 ,  H01B5/14 ,  C12P21/00 ,  C08L83/04 ,  C08L89/00 ,  A61B5/145 ,  A61B5/00 ,  C08L29/04 ,  C07K14/195 ,  C09D129/04 ,  C09D183/04 ,  B82Y30/00 ,  B82Y5/00

CPC classification number: H01B1/12 ,  A61B5/14539 ,  A61B5/6801 ,  B82Y5/00 ,  B82Y30/00 ,  C07K14/195 ,  C08L29/04 ,  C08L83/04 ,  C08L89/00 ,  C09D129/04 ,  C09D183/04 ,  C12P21/00 ,  H01B3/44 ,  H01B5/14 ,  A61B2562/0285 ,  C08L29/04 ,  C08L89/00 ,  C09D129/04 ,  C08L89/00 ,  C08L83/04 ,  C08L89/00

Abstract: Electrically conductive polymeric composite materials include microbially produced protein nanowires. The conductive composites are useful in diverse electronic materials applications, particularly in applications requiring biocompatibility, such as sensors and wearable electronics.

公开(公告)号：US20230040959A1

公开(公告)日：2023-02-09

申请号：US17778769

申请日：2020-11-20

Applicant: University of Massachusetts

Inventor： Derek R. Lovley ,  Toshiyuki Ueki ,  David Walker ,  Trevor Woodard ,  Kelly Nevin Lovley

IPC: C07K14/245 ,  C12N15/72 ,  C12N15/52

Abstract: The present invention provides, in various embodiments, genetically modified aerobic bacteria, polynucleotides and methods for expressing and/or harvesting electrically conductive protein nanowires (e-PNs). The present invention also provides e-PNs produced using the genetically modified aerobic bacteria, polynucleotides and methods.

公开(公告)号：US20230160885A1

公开(公告)日：2023-05-25

申请号：US17440632

申请日：2020-03-20

Applicant: University of Massachusetts

Inventor： Derek R. Lovley ,  Toshiyuki Ueki

IPC: G01N33/543 ,  C07K14/195

CPC classification number: G01N33/5438 ,  C07K14/195 ,  C07K2319/42

Abstract: The present invention provides, in various embodiments, electrically conductive protein nanowires (e-PNs) having surface exposed peptides that confer additional sensing capabilities and/or enhance binding to other materials, as well as fusion proteins and methods for making such nanowires. The present invention also provides sensor devices comprising the nanowires.

公开(公告)号：US11631824B2

公开(公告)日：2023-04-18

申请号：US17226016

申请日：2021-04-08

Applicant: University of Massachusetts

Inventor： Jun Yao ,  Derek R. Lovley ,  Tianda Fu

IPC: G11C11/54 ,  H01L51/05 ,  G11C13/00 ,  H01L51/00

Abstract: A memristive device includes a biomaterial comprising protein nanowires and at least two electrodes in operative arrangement with the biomaterial such that an applied voltage induces conductance switching. An artificial neuron or an artificial synapse includes a memrisitive device with the electrodes configured to apply a pulsed voltage configured to mimic an action-potential input.

公开(公告)号：US11066449B2

公开(公告)日：2021-07-20

申请号：US15744116

申请日：2016-07-19

Applicant: University of Massachusetts

Inventor： Derek R. Lovley ,  Nikhil Malvankar ,  Ramesh Adhikari ,  Yang Tan ,  Joy Ward ,  Kelly Nevin

IPC: C07K14/195 ,  H01B1/12

Abstract: Improved electrically conductive pili were generated from a natural pilus protein from the microorganism Geobacter sulfurreducens. Substituting a tryptophan for the phenylalanine at position F51 and a tryptophan for the tyrosine at position Y57 of the pilus monomer substantially increased the conductivity of the pili and reduced their diameter to 1.5 nm. Substantial improvements in conductivity were also achieved by substituting an additional tyrosine, histidine, and phenylalanine in the pilus monomer to mimic the monomer of Geobacter metallireducens, but the pili retained the typical Geobacter sulfurreducens wild-type diameter of 3 nm.