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    FLUIDIC CONNECTORS AND MICROFLUIDIC SYSTEMS
    4.
    发明申请
    FLUIDIC CONNECTORS AND MICROFLUIDIC SYSTEMS 有权
    流体连接器和微流体系统

    公开(公告)号：US20080273918A1

    公开(公告)日：2008-11-06

    申请号：US12113503

    申请日：2008-05-01

    申请人： Vincent Linder ,  David Steinmiller ,  Samuel K. Sia

    发明人： Vincent Linder ,  David Steinmiller ,  Samuel K. Sia

    IPC分类号： F16D3/80

    CPC分类号： G01N33/54366 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/563 ,  B01L3/565 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/041 ,  B01L2300/0627 ,  B01L2300/0672 ,  B01L2300/0838 ,  B01L2300/0861 ,  B01L2300/12 ,  B01L2400/049 ,  C12Q1/6834 ,  G01N21/6428 ,  G01N33/5302 ,  G01N33/5304 ,  G01N33/5306 ,  G01N33/545 ,  Y10T403/22 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575

    摘要： Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods amounts of time (e.g., 1 year) prior to use. Prior to connection of the fluid connector and the substrate, the fluid path may be filled with a sample (e.g., blood). The sample may be obtained, for example, by pricking a finger of a user until blood is drawn from the finger into the fluid path (e.g., by capillary forces). Upon connection of the fluidic connector and the channels of the substrate, the sample can pass through a reaction area within the first channel of the substrate. This process can allow components of the sample to interact with components disposed in the reaction area. Afterwards, reagents from the second channel can flow to the reaction area via the fluid path, allowing components in the reaction area to be processed (e.g., amplified to produce detectable signal). Components in the reaction area can then be determined using various methods of detection.

    摘要翻译： 提供了用于在微流体系统中进行分析（例如免疫测定）的流体连接器，方法和装置。 在一些实施例中，具有流体路径的流体连接器用于连接形成在基板中的两个独立通道，以允许两个独立通道之间的流体连通。 可以使用可用于进行分析的试剂（例如，抗体溶液，洗涤缓冲液和扩增试剂）预先填充一个或两个独立通道。 这些试剂可以在使用前长时间（例如1年）存储在基质的通道中。 在连接流体连接器和基底之前，流体路径可以填充有样品（例如血液）。 样品可以例如通过穿刺用户的手指而获得，直到血液从手指拉入流体路径（例如通过毛细血管力）。 在连接流体连接器和衬底的通道时，样品可以通过衬底的第一通道内的反应区域。 该过程可以允许样品的组分与设置在反应区域中的组分相互作用。 之后，来自第二通道的试剂可以经由流体路径流到反应区域，从而允许处理反应区域中的组分（例如，被扩增以产生可检测的信号）。 然后可以使用各种检测方法来确定反应区域中的组分。

    Fluidic connectors and microfluidic systems
    5.
    发明授权
    Fluidic connectors and microfluidic systems 有权
    流体连接器和微流体系统

    公开(公告)号：US08202492B2

    公开(公告)日：2012-06-19

    申请号：US12113503

    申请日：2008-05-01

    申请人： Vincent Linder ,  David Steinmiller ,  Samuel K. Sia

    发明人： Vincent Linder ,  David Steinmiller ,  Samuel K. Sia

    IPC分类号： G01N15/06 ,  G01N33/00 ,  G01N33/48

    CPC分类号： G01N33/54366 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/563 ,  B01L3/565 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/041 ,  B01L2300/0627 ,  B01L2300/0672 ,  B01L2300/0838 ,  B01L2300/0861 ,  B01L2300/12 ,  B01L2400/049 ,  C12Q1/6834 ,  G01N21/6428 ,  G01N33/5302 ,  G01N33/5304 ,  G01N33/5306 ,  G01N33/545 ,  Y10T403/22 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575

    摘要： Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods amounts of time (e.g., 1 year) prior to use. Prior to connection of the fluid connector and the substrate, the fluid path may be filled with a sample (e.g., blood). The sample may be obtained, for example, by pricking a finger of a user until blood is drawn from the finger into the fluid path (e.g., by capillary forces). Upon connection of the fluidic connector and the channels of the substrate, the sample can pass through a reaction area within the first channel of the substrate. This process can allow components of the sample to interact with components disposed in the reaction area. Afterwards, reagents from the second channel can flow to the reaction area via the fluid path, allowing components in the reaction area to be processed (e.g., amplified to produce detectable signal). Components in the reaction area can then be determined using various methods of detection.

    摘要翻译： 提供了用于在微流体系统中进行分析（例如免疫测定）的流体连接器，方法和装置。 在一些实施例中，具有流体路径的流体连接器用于连接形成在基板中的两个独立通道，以允许两个独立通道之间的流体连通。 可以使用可用于进行分析的试剂（例如，抗体溶液，洗涤缓冲液和扩增试剂）预先填充一个或两个独立通道。 这些试剂可以在使用前长时间（例如1年）存储在基质的通道中。 在连接流体连接器和基底之前，流体路径可以填充有样品（例如血液）。 样品可以例如通过穿刺用户的手指而获得，直到血液从手指拉入流体路径（例如通过毛细血管力）。 在连接流体连接器和衬底的通道时，样品可以通过衬底的第一通道内的反应区域。 该过程可以允许样品的组分与设置在反应区域中的组分相互作用。 之后，来自第二通道的试剂可以经由流体路径流到反应区域，从而允许处理反应区域中的组分（例如，被扩增以产生可检测的信号）。 然后可以使用各种检测方法来确定反应区域中的组分。

    Assay device and method
    7.
    发明授权
    Assay device and method 有权
    测定装置和方法

    公开(公告)号：US08574924B2

    公开(公告)日：2013-11-05

    申请号：US12769489

    申请日：2010-04-28

    申请人： Samuel K. Sia ,  Vincent Linder ,  Babak Amir-Parviz ,  Adam Siegel ,  George M. Whitesides

    发明人： Samuel K. Sia ,  Vincent Linder ,  Babak Amir-Parviz ,  Adam Siegel ,  George M. Whitesides

    IPC分类号： G01N33/553

    CPC分类号： G01N33/56988 ,  B01L3/502707 ,  B01L2300/0822 ,  B01L2300/168 ,  G01N21/05 ,  G01N21/59 ,  G01N33/5302 ,  G01N33/543 ,  G01N33/585 ,  G01N2021/0346 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339

    摘要： An assay method is described, which comprises the steps of immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion of a surface of a microfluidic chamber; passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold-conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface.

    摘要翻译： 描述了一种测定方法，其包括将待检测分析物（例如抗体或抗原）的结合配偶体（例如，抗原或抗体）固定在微流体室表面的一部分上的步骤; 将流体样品通过表面并允许分析物结合到结合配偶体; 允许金属胶体，例如金共轭抗体与结合的分析物缔合; 在表面上流动金属溶液，例如银溶液，以形成不透明的金属层; 并且例如通过目视检查或通过在将金属溶液流过表面之后测量金属溶液中的层的透光率，层的导电性或电阻或金属浓度来检测所述金属层的存在。

    ASSAY DEVICE AND METHOD
    8.
    发明申请
    ASSAY DEVICE AND METHOD 有权
    测定装置和方法

    公开(公告)号：US20100279310A1

    公开(公告)日：2010-11-04

    申请号：US12769489

    申请日：2010-04-28

    申请人： Samuel K. Sia ,  Vincent Linder ,  Adam Siegel ,  George M. Whitesides ,  Babak Amir-Parviz

    发明人： Samuel K. Sia ,  Vincent Linder ,  Adam Siegel ,  George M. Whitesides ,  Babak Amir-Parviz

    IPC分类号： G01N33/53

    CPC分类号： G01N33/56988 ,  B01L3/502707 ,  B01L2300/0822 ,  B01L2300/168 ,  G01N21/05 ,  G01N21/59 ,  G01N33/5302 ,  G01N33/543 ,  G01N33/585 ,  G01N2021/0346 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339

    摘要： An assay method is described, which comprises the steps of immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion of a surface of a microfluidic chamber; passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold-conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface.

    摘要翻译： 描述了一种测定方法，其包括将待检测分析物（例如抗体或抗原）的结合配偶体（例如，抗原或抗体）固定在微流体室表面的一部分上的步骤; 将流体样品通过表面并允许分析物结合到结合配偶体; 允许金属胶体，例如金共轭抗体与结合的分析物缔合; 在表面上流动金属溶液，例如银溶液，以形成不透明的金属层; 并且例如通过目视检查或通过在将金属溶液流过表面之后测量金属溶液中的层的透光率，层的导电性或电阻或金属浓度来检测所述金属层的存在。