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公开(公告)号：US08574924B2
公开(公告)日：2013-11-05
申请号：US12769489
申请日：2010-04-28
IPC分类号： G01N33/553
CPC分类号： G01N33/56988 , B01L3/502707 , B01L2300/0822 , B01L2300/168 , G01N21/05 , G01N21/59 , G01N33/5302 , G01N33/543 , G01N33/585 , G01N2021/0346 , Y10T436/11 , Y10T436/117497 , Y10T436/118339
摘要： An assay method is described, which comprises the steps of immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion of a surface of a microfluidic chamber; passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold-conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface.
摘要翻译： 描述了一种测定方法，其包括将待检测分析物（例如抗体或抗原）的结合配偶体（例如，抗原或抗体）固定在微流体室表面的一部分上的步骤; 将流体样品通过表面并允许分析物结合到结合配偶体; 允许金属胶体，例如金共轭抗体与结合的分析物缔合; 在表面上流动金属溶液，例如银溶液，以形成不透明的金属层; 并且例如通过目视检查或通过在将金属溶液流过表面之后测量金属溶液中的层的透光率，层的导电性或电阻或金属浓度来检测所述金属层的存在。
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公开(公告)号：US20100279310A1
公开(公告)日：2010-11-04
申请号：US12769489
申请日：2010-04-28
IPC分类号： G01N33/53
CPC分类号： G01N33/56988 , B01L3/502707 , B01L2300/0822 , B01L2300/168 , G01N21/05 , G01N21/59 , G01N33/5302 , G01N33/543 , G01N33/585 , G01N2021/0346 , Y10T436/11 , Y10T436/117497 , Y10T436/118339
摘要： An assay method is described, which comprises the steps of immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion of a surface of a microfluidic chamber; passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold-conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface.
摘要翻译： 描述了一种测定方法，其包括将待检测分析物（例如抗体或抗原）的结合配偶体（例如，抗原或抗体）固定在微流体室表面的一部分上的步骤; 将流体样品通过表面并允许分析物结合到结合配偶体; 允许金属胶体，例如金共轭抗体与结合的分析物缔合; 在表面上流动金属溶液，例如银溶液，以形成不透明的金属层; 并且例如通过目视检查或通过在将金属溶液流过表面之后测量金属溶液中的层的透光率，层的导电性或电阻或金属浓度来检测所述金属层的存在。
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公开(公告)号：US08501416B2
公开(公告)日：2013-08-06
申请号：US11887487
申请日：2006-04-19
IPC分类号： G01N33/53
CPC分类号： G01N33/54366 , B01L3/502746 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0883 , B01L2400/0406 , G01N21/01 , G01N21/82 , G01N33/54373
摘要： The present invention relates generally to microfluidic structures, and more specifically, to microfluidic structures and methods including meandering and wide channels. Microfluidic systems can provide an advantageous environment for performing various reactions and analysis due to a reduction in sample and reagent quantities that are required, a reduction in the size of the operating system, and a decrease in reaction time compared to conventional systems. Unfortunately, the small size of microfluidic channels can sometimes result in difficulty in detecting a species without magnifying optics (such as a microscope or a photomultiplier). A series of tightly packed microchannels, i.e., a meandering region, or a wide channel having a dimension on the order of millimeters, can serve as a solution to this problem by creating a wide measurement area. Although this invention mainly describes the use of meandering and wide channels in heterogeneous immunoassays on a microfluidic chip, this invention could be used for amplifying optical signals for other types of reactions and/or assays.
摘要翻译： 本发明一般涉及微流体结构，更具体地涉及包括曲折和宽通道的微流体结构和方法。 微流控系统可以提供用于执行各种反应和分析的有利环境，因为与常规系统相比，所需的样品和试剂量的减少，操作系统的尺寸的减小和反应时间的减少。 不幸的是，微量流体通道的小尺寸有时会导致在不放大光学器件（例如显微镜或光电倍增管）的情况下检测物质的困难。 一系列紧密包装的微通道，即曲折区域或具有大约毫米数量级的宽通道可以通过创建宽的测量区域来解决这个问题。 尽管本发明主要描述了在微流体芯片上的异源免疫测定中使用曲折和宽通道，但是本发明可用于放大用于其他类型反应和/或测定的光学信号。
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公开(公告)号：US20110315229A1
公开(公告)日：2011-12-29
申请号：US13226154
申请日：2011-09-06
IPC分类号： F15D1/00
CPC分类号： G01N33/5304 , B01L3/0293 , B01L3/5027 , B01L3/502784 , B01L2200/0673 , B01L2200/16 , B01L2300/0816 , B82Y30/00 , G01N33/543 , G01N35/08 , G01N35/1002 , G01N35/1065 , Y10T137/0318
摘要： A method and apparatus for delivering one or more fluids. Fluids may be delivered sequentially from a common vessel to a chemical, biological or biochemical process.
摘要翻译： 一种用于递送一种或多种流体的方法和装置。 流体可以从普通容器依次递送到化学，生物或生化过程。
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公开(公告)号：US07741014B2
公开(公告)日：2010-06-22
申请号：US11401485
申请日：2006-04-10
申请人： Declan Ryan , Babak Amir-Parviz , Vincent Linder , Vincent Semetey , Samuel K. Sia , George M. Whitesides
发明人： Declan Ryan , Babak Amir-Parviz , Vincent Linder , Vincent Semetey , Samuel K. Sia , George M. Whitesides
IPC分类号： G03F7/00 , H01L21/302
CPC分类号： G03F7/165 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00628 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B82Y5/00 , B82Y30/00 , C03C17/28 , C03C23/0005 , C03C2218/32 , C40B80/00 , G03F7/203
摘要： A series of methods, compositions, and articles for patterning a surface with multiple, aligned layers of molecules, by exposing the molecules to electromagnetic radiation is provided. In certain embodiments, a single photomask acts as an area-selective filter for light at multiple wavelengths. A single set of exposures of multiple wavelengths through this photomask may make it possible to fabricate a pattern comprising discontinuous multiple regions, where the regions differ from each other in at least one chemical and/or physical property, without acts of alignment between the exposures. In certain embodiments, the surface includes molecules attached thereto that can be photocleaved upon exposure to a certain wavelength of radiation, thereby altering the chemical composition on at least a portion of the surface.
摘要翻译： 提供了一系列用于通过将分子暴露于电磁辐射来图案化具有多个对准的分子层的表面的方法，组合物和制品。 在某些实施例中，单个光掩模用作多个波长的光的区域选择滤光器。 通过该光掩模的多组波长的一组曝光可以使得可以制造包括不连续多个区域的图案，其中区域在至少一种化学和/或物理性质上彼此不同，而不影响曝光之间的对准。 在某些实施方案中，表面包括附着于其上的分子，其可以在暴露于一定波长的辐射时被光刻，从而改变表面的至少一部分上的化学组成。
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公开(公告)号：US08030057B2
公开(公告)日：2011-10-04
申请号：US10587156
申请日：2005-01-26
IPC分类号： C12M1/34
CPC分类号： B01L3/0293 , B01L3/50273 , B01L3/502738 , B01L3/502784 , B01L2200/0673 , B01L2200/16 , B01L2300/0816 , B01L2300/0877 , B01L2400/0487 , G01N21/11 , G01N33/54366 , G01N33/54386 , G01N33/553 , G01N35/08 , G01N35/1002 , Y10T436/25
摘要： Method and device for storing and/or delivering fluids, wherein at least a first and a second fluid, such as chemical or biochemical reagents or rinse solutions, are maintained separately from each other in a common vessel and transferred in series from the vessel to a reaction site to carry out a predetermined chemical or biochemical reaction. Separation may be achieved by interposing a third fluid, e.g., a gaseous fluid plug, between the first and second fluids.
摘要翻译： 用于储存和/或输送流体的方法和装置，其中至少第一和第二流体，例如化学或生物化学试剂或冲洗溶液在共同的容器中彼此分开地保持，并从容器串联转移到 反应部位进行预定的化学反应或生化反应。 分离可以通过在第一和第二流体之间插入第三流体，例如气体流体塞。
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公开(公告)号：US08389272B2
公开(公告)日：2013-03-05
申请号：US13226154
申请日：2011-09-06
IPC分类号： C12M1/34
CPC分类号： G01N33/5304 , B01L3/0293 , B01L3/5027 , B01L3/502784 , B01L2200/0673 , B01L2200/16 , B01L2300/0816 , B82Y30/00 , G01N33/543 , G01N35/08 , G01N35/1002 , G01N35/1065 , Y10T137/0318
摘要： A method and apparatus for delivering one or more fluids. Fluids may be delivered sequentially from a common vessel to a chemical, biological or biochemical process.
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公开(公告)号：US07736890B2
公开(公告)日：2010-06-15
申请号：US10584819
申请日：2004-12-29
CPC分类号： G01N33/56988 , B01L3/502707 , B01L2300/0822 , B01L2300/168 , G01N21/05 , G01N21/59 , G01N33/5302 , G01N33/543 , G01N33/585 , G01N2021/0346 , Y10T436/11 , Y10T436/117497 , Y10T436/118339
摘要： An assay method is described, which comprises the steps of immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion (140) of a surface (130) of a microfluidic chamber (120,122,124); passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold-conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface.
摘要翻译： 描述了测定方法，其包括以下步骤：将待检测分析物（例如，抗体或抗原）的结合配偶体（例如，抗原或抗体）固定在表面（130）的部分（140）上 微流体室（120,122,124）; 将流体样品通过表面并允许分析物结合到结合配偶体; 允许金属胶体，例如金共轭抗体与结合的分析物缔合; 在表面上流动金属溶液，例如银溶液，以形成不透明的金属层; 并且例如通过目视检查或通过在将金属溶液流过表面之后测量金属溶液中的层的透光率，层的导电性或电阻或金属浓度来检测所述金属层的存在。
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公开(公告)号：US20120238033A1
公开(公告)日：2012-09-20
申请号：US13467653
申请日：2012-05-09
申请人： Vincent Linder , David Steinmiller , Samuel K. Sia
发明人： Vincent Linder , David Steinmiller , Samuel K. Sia
CPC分类号： G01N33/54366 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L3/563 , B01L3/565 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2200/16 , B01L2300/021 , B01L2300/041 , B01L2300/0627 , B01L2300/0672 , B01L2300/0838 , B01L2300/0861 , B01L2300/12 , B01L2400/049 , C12Q1/6834 , G01N21/6428 , G01N33/5302 , G01N33/5304 , G01N33/5306 , G01N33/545 , Y10T403/22 , Y10T436/11 , Y10T436/25 , Y10T436/2575
摘要： Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods amounts of time (e.g., 1 year) prior to use.
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公开(公告)号：US08475737B2
公开(公告)日：2013-07-02
申请号：US13467627
申请日：2012-05-09
申请人： Vincent Linder , David Steinmiller , Samuel K. Sia
发明人： Vincent Linder , David Steinmiller , Samuel K. Sia
CPC分类号： G01N33/54366 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L3/563 , B01L3/565 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2200/16 , B01L2300/021 , B01L2300/041 , B01L2300/0627 , B01L2300/0672 , B01L2300/0838 , B01L2300/0861 , B01L2300/12 , B01L2400/049 , C12Q1/6834 , G01N21/6428 , G01N33/5302 , G01N33/5304 , G01N33/5306 , G01N33/545 , Y10T403/22 , Y10T436/11 , Y10T436/25 , Y10T436/2575
摘要： Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods amounts of time (e.g., 1 year) prior to use.
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