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    Process for concentrating nucleic acid molecules
    2.
    发明授权
    Process for concentrating nucleic acid molecules 有权
    浓缩核酸分子的方法

    公开(公告)号：US08975017B2

    公开(公告)日：2015-03-10

    申请号：US12734934

    申请日：2008-11-03

    申请人： Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel

    发明人： Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel

    IPC分类号： C12Q1/68 ,  G01N33/48

    CPC分类号： C12Q1/6806 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/6837 ,  Y10T436/143333 ,  C12Q2527/107 ,  C12Q2537/125 ,  C12Q2565/543

    摘要： A process concentrates nucleic acid molecules to be detected of a sample on a surface, with capture molecules that specifically bind the nucleic acid molecules. A support material has a capture probe that can specifically be linked to the nucleic acid molecules to be detected to give complexes. The support material and the nucleic acid molecules of the sample are incubated to form the complexes. The complexes are moved to the surface. At least one portion of the complexes becomes bound to the capture molecules via the capture probe.

    摘要翻译： 一种方法将待检测的样品的核酸分子浓缩在表面上，其中捕获分子特异性结合核酸分子。 载体材料具有捕获探针，其可以特异性地连接到待检测的核酸分子以得到复合物。 将样品的载体材料和核酸分子孵育以形成复合物。 络合物移动到表面。 复合物的至少一部分经由捕获探针与捕获分子结合。

    Apparatus and method comprising a sensor array and a porous plunger and use thereof
    3.
    发明授权
    Apparatus and method comprising a sensor array and a porous plunger and use thereof 有权
    包括传感器阵列和多孔柱塞的装置和方法及其用途

    公开(公告)号：US08753582B2

    公开(公告)日：2014-06-17

    申请号：US12867561

    申请日：2009-02-09

    申请人： Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel

    发明人： Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel

    IPC分类号： G01N35/00 ,  G01N31/22 ,  G01N33/52 ,  B01L3/00 ,  G01N31/16 ,  B01L99/00 ,  G01N30/62 ,  G01N30/95 ,  G01N33/00 ,  C12M1/34 ,  C12M3/00

    CPC分类号： G01N33/5438 ,  B01J2219/00382 ,  B01J2219/00527 ,  B01J2219/00704 ,  B01L3/502707 ,  B01L3/502715 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/087 ,  B01L2400/0421 ,  B01L2400/0427 ,  G01N27/27 ,  G01N27/3277

    摘要： Disclosed is an apparatus that is used for detecting liquids or substances from liquids and includes a plunger that has a porous plunger layer which is pressed onto a sensor array. Each sensor of the sensor array is surrounded by elevations which spatially separate the sensors from each other like walls. In at least one embodiment, when the plunger layer is pressed onto the sensor array, the walls are pressed into the pores of the plunger layer. Liquid-tight connections are created between the walls and the plunger layer while liquid remains over the sensors. The liquid can be measured. In at least one embodiment, when there is direct mechanical contact between the plunger layer and the sensors, the liquid over the sensors is located in open pores on the surface of the plunger layer. No liquid flows between the pores and across the walls when pressure is applied to the plunger layer such that closed reaction chambers are created.

    摘要翻译： 公开了一种用于从液体中检测液体或物质的装置，包括具有压在传感器阵列上的多孔柱塞层的柱塞。 传感器阵列的每个传感器被高度包围，空间上将传感器彼此像墙壁分开。 在至少一个实施例中，当柱塞层被压到传感器阵列上时，壁压入柱塞层的孔中。 在壁和柱塞层之间产生液密连接，而液体保留在传感器上方。 可以测量液体。 在至少一个实施例中，当柱塞层和传感器之间存在直接机械接触时，传感器上的液体位于柱塞层表面上的开放孔中。 当压力施加到柱塞层时，没有液体流过孔隙和壁之间，从而形成封闭的反应室。

    Method for detecting DNA point mutations (single nucleotide polymorphism (SNP) analysis) and associated arrangement
    5.
    发明授权
    Method for detecting DNA point mutations (single nucleotide polymorphism (SNP) analysis) and associated arrangement 有权
    DNA点突变检测方法（单核苷酸多态性（SNP）分析）及相关安排

    公开(公告)号：US07488578B2

    公开(公告)日：2009-02-10

    申请号：US10558709

    申请日：2004-05-28

    申请人： Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel

    发明人： Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： B01L3/5027 ,  B01L7/52 ,  B01L2200/10 ,  C12Q1/6837

    摘要： The single nucleotide polymorphism analysis involves the utilization of a DNA hybridization process as well as the use of a DNA chip. A liquid DNA sample to be analyzed is guided over a DNA chip in a defined time course. After successful hybridization, the temperature is modified in a defined manner under low stringency conditions such that scavenger/target DNA hybrids are melted, whereby the melting of the scavenger/target DNA hybrids is detected and evaluated according to the temperature. In addition to the DNA chip, at least one device is provided that controls and regulates the temperature, and another device is provided that controls a lateral flow of liquid against the surface of the DNA chip. Factors for matching hybrids and mismatching/single point mismatching hybrids can be detected and evaluated using appropriate measuring device(s).

    摘要翻译： 单核苷酸多态性分析涉及利用DNA杂交过程以及使用DNA芯片。 将待分析的液体DNA样品在规定的时间过程中引导到DNA芯片上。 成功杂交后，在低严格条件下以限定的方式修饰温度，使得清除剂/靶DNA杂交体被熔化，从而根据温度检测和评价清除剂/靶DNA杂交体的融化。 除了DNA芯片之外，还提供了至少一个控制和调节温度的装置，并且提供另一个装置来控制液体相对于DNA芯片表面的横向流动。 可以使用适当的测量装置检测和评估匹配杂交体和错配/单点不匹配杂交体的因子。

    Electrochemical Transducer Array and Use Thereof
    6.
    发明申请
    Electrochemical Transducer Array and Use Thereof 有权
    电化学传感器阵列及其应用

    公开(公告)号：US20080283397A1

    公开(公告)日：2008-11-20

    申请号：US10587745

    申请日：2005-01-26

    申请人： Heike Barlag ,  Walter Gumbrecht ,  Manfred Stanzel

    发明人： Heike Barlag ,  Walter Gumbrecht ,  Manfred Stanzel

    IPC分类号： G01N33/487

    CPC分类号： G01N27/403

    摘要： Electrochemical transducer arrays are already known from the prior art. According to the invention, the transducer array is provided with at least one flexible, planar metal substrate on which at least one flexible insulator having a firm connection between the metal surface and the insulator surface is disposed. The metal substrate and the insulator disposed thereon are structured in such a manner as to give metal areas which are electrically insulated the one from the other and which serve as sensor areas. The metal substrate used is self-contained so that the structured metal areas can be contacted from the lower side.

    摘要翻译： 电化学换能器阵列从现有技术已经是已知的。 根据本发明，换能器阵列设置有至少一个柔性的平面金属基底，其上布置有在金属表面和绝缘体表面之间具有牢固连接的至少一个柔性绝缘体。 金属基板和设置在其上的绝缘体以使得彼此电绝缘并用作传感器区域的金属区域的方式构造。 使用的金属基板是独立的，使得结构化金属区域可以从下侧接触。

    Pcr Process And Arrangement For Dna Amplification Using Dry Reagents
    7.
    发明申请
    Pcr Process And Arrangement For Dna Amplification Using Dry Reagents 有权
    使用干试剂进行Dna扩增的Pcr工艺和布置

    公开(公告)号：US20080241890A1

    公开(公告)日：2008-10-02

    申请号：US11587567

    申请日：2005-04-26

    申请人： Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel

    发明人： Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel

    IPC分类号： C12P19/34

    CPC分类号： C12Q1/686

    摘要： A PCR process for DNA amplification with thermocyclisation of the corresponding reagents is disclosed, in which total integration of all substances and process steps is achieved in a closed, single-use unit (so-called cartridge) in which the reagents are stored in a storage-stable form at room temperature. According to the process, the water-soluble reagents are covered by a water-insoluble medium, then the DNA to be amplified is supplied and the water-insoluble medium is eliminated, so that the water-soluble reagents are dissolved and PCR can start. In the corresponding arrangement, a test unit designed as a single-use produce (a so-called cartridge) has at least one micro-channel or micro-cavity for receiving a PCR reagent. The PCR reagents in the form of a mixture which can be dried at a negligible vapour pressure and forms a storage-stable substance substance at room temperature adhere to the walls of the micro-channel or micro-cavity and form a thin film covered by an insoluble medium.

    摘要翻译： 公开了用于相应试剂的热循环的DNA扩增的PCR方法，其中所有物质和方法步骤的完全整合在封闭的一次性使用单元（所谓的盒）中实现，其中试剂存储在储存器 - 稳定形式在室温下。 根据该方法，水溶性试剂被水不溶性介质覆盖，然后供给待扩增的DNA，除去水不溶性介质，溶解水溶性试剂并开始PCR。 在相应的布置中，设计为单次使用的产品（所谓的盒）的测试单元具有用于接收PCR试剂的至少一个微通道或微腔。 可以以可忽略的蒸气压干燥并在室温下形成储存稳定物质物质的混合物形式的PCR试剂粘附到微通道或微腔的壁上并形成由 不溶性介质。