利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

145 条记录 (耗时 0.027 秒)

    Automatic positioning and sensing microelectrode arrays
    5.
    发明授权
    Automatic positioning and sensing microelectrode arrays 有权
    自动定位和感应微电极阵列

    公开(公告)号：US08784633B2

    公开(公告)日：2014-07-22

    申请号：US12667507

    申请日：2007-07-04

    申请人： Guangxin Xiang ,  Liangbin Pan ,  Wanli Xing ,  Lihua Huang ,  Zhongyao Yu ,  Jing Zhu ,  Yuxiang Zhou ,  Jing Cheng

    发明人： Guangxin Xiang ,  Liangbin Pan ,  Wanli Xing ,  Lihua Huang ,  Zhongyao Yu ,  Jing Zhu ,  Yuxiang Zhou ,  Jing Cheng

    IPC分类号： G01N27/447 ,  G01N27/453

    CPC分类号： G01N33/4836 ,  B01J2219/00653 ,  B01J2219/00743 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2400/0421 ,  B01L2400/0424

    摘要： A microelectrode sensing device includes a substrate and an array of microelectrode sensors. Each sensor includes a first conductive layer that at least partially conducts electricity. The first conductive layer is formed above the substrate and patterned to include a recording electrode that measures electrical activities of target cells. Each sensor also includes a second conductive layer that at least partially conducts electricity. The second conductive layer is elevated above the first layer and patterned to include multiple positioning electrodes arranged to define a sensing region above the recording electrode. The positioning electrodes are designed to generate an electric field pattern in the sensing region to move and confine the target cells to a sub-region of the sensing region that at least partially overlaps the recording electrode.

    摘要翻译： 微电极感测装置包括基板和微电极传感器阵列。 每个传感器包括至少部分地导电的第一导电层。 第一导电层形成在衬底上并被图案化以包括测量靶细胞的电活动的记录电极。 每个传感器还包括至少部分地传导电的第二导电层。 第二导电层在第一层之上被升高并且被图案化以包括布置成限定记录电极上方的感测区域的多个定位电极。 定位电极被设计成在感测区域中产生电场图案以移动并将目标单元限制在至少部分地与记录电极重叠的感测区域的子区域上。

    Apparatus for stimulating an animal cell and recording its physiological signal and production and use methods thereof
    6.
    发明授权
    Apparatus for stimulating an animal cell and recording its physiological signal and production and use methods thereof 有权
    用于刺激动物细胞并记录其生理信号的装置及其生产和使用方法

    公开(公告)号：US07632674B2

    公开(公告)日：2009-12-15

    申请号：US10499098

    申请日：2002-11-29

    申请人： Wanli Xing ,  Zhe Yu ,  Guangxin Xiang ,  Liangbin Pan ,  Jing Cheng

    发明人： Wanli Xing ,  Zhe Yu ,  Guangxin Xiang ,  Liangbin Pan ,  Jing Cheng

    IPC分类号： C12M3/00

    CPC分类号： G01N33/5438 ,  C12M35/02 ,  G01N2333/46 ,  G01N2500/10

    摘要： The present invention provides an apparatus for stimulating an animal cell and recording its physiological signal and methods of making and using thereof. The purpose of the present invention is to provide an apparatus for stimulating an animal cell and recording its physiological signal that is efficient, convenient, and accurate. The apparatus for stimulating an animal cell and recording its physiological signal of the present invention comprises a poor conductive substrate, wherein on at least one surface of the substrate is provided at least one unit conductive polymer layer and at least one good conductive microelectrode.

    摘要翻译： 本发明提供一种用于刺激动物细胞并记录其生理信号的装置及其制造和使用方法。 本发明的目的是提供一种用于刺激动物细胞并记录其生理信号的装置，其有效，方便和准确。 用于刺激动物细胞并记录其本发明的生理信号的装置包括差的导电基底，其中在基底的至少一个表面上提供至少一个单位导电聚合物层和至少一个良好的导电微电极。

    Method and apparatus for washing solid substrate with ultrasonic wave after hybridization reaction
    8.
    发明申请
    Method and apparatus for washing solid substrate with ultrasonic wave after hybridization reaction 审中-公开
    杂交反应后用超声波洗涤固体底物的方法和装置

    公开(公告)号：US20060124152A1

    公开(公告)日：2006-06-15

    申请号：US10524903

    申请日：2002-12-10

    申请人： Gang Li ,  Wanli Xing ,  Ruige Wu ,  Min Guo ,  Jing Cheng

    发明人： Gang Li ,  Wanli Xing ,  Ruige Wu ,  Min Guo ,  Jing Cheng

    IPC分类号： B08B3/10

    CPC分类号： B08B3/12 ,  C12Q1/6813 ,  C12Q2523/301

    摘要： A method and apparatus for washing solid substrate with ultrasonic wave after hybridization reaction are provided. The method includes (1) putting the solid substrate into a container having a washing solution, on which the hybridization reaction has been completed, (2) using an ultrasonic generator to produce a certain strength of ultrasonic wave and transmit it into the washing solution, (3) washing the substrate by means of the cavitation effect being produced in the washing solution by the ultrasonic wave. Since the cavitation bubbles in the washing solution can produce intensive efflux and local microslipstream against the solid surface, evidently reducing the liquid surface tension and friction and enhance the liquid flow, non-specific adsorbate and deposit bound weakly on the solid substrate can be washed down rapidly.

    摘要翻译： 提供了一种用于在杂交反应之后用超声波洗涤固体底物的方法和装置。 该方法包括：（1）将固体基质置于具有杂交反应完成的洗涤液的容器中，（2）使用超声波发生器产生一定强度的超声波并将其输送到洗涤液中， （3）通过超声波在洗涤液中产生的空化作用洗涤基板。 由于洗涤液中的气穴气泡能够对固体表面产生强烈的流出和局部微滑流，明显降低了液面张力和摩擦力，增强了液体流动，固体基质上弱结合的非特异性吸附物和沉积物可以被冲洗掉 迅速

    Microfluidic device and its use for positioning of cells or organisms
    9.
    发明授权
    Microfluidic device and its use for positioning of cells or organisms 有权
    微流体装置及其用于定位细胞或生物体的用途

    公开(公告)号：US09511366B2

    公开(公告)日：2016-12-06

    申请号：US13991848

    申请日：2012-01-13

    申请人： Rui Ma ,  Lan Xie ,  Tian Qiu ,  Lei Wang ,  Wanli Xing ,  Jing Cheng

    发明人： Rui Ma ,  Lan Xie ,  Tian Qiu ,  Lei Wang ,  Wanli Xing ,  Jing Cheng

    IPC分类号： B01L3/00 ,  C12M3/00 ,  C12M1/32 ,  C12M3/06

    CPC分类号： B01L3/5027 ,  C12M21/06 ,  C12M23/12 ,  C12M23/16

    摘要： A micro fluidic device comprises one microstructure layer (5) and one cover layer (1), wherein the cover layer (1) is connected to the microstructure layer (5). The microstructure layer (5) comprises one bottom layer and a plurality of microstructures on it to position samples. The cover layer (1) comprises one top layer, one positioning well (6) and at least one inlet pool (4). The positioning well (6) is right above the microstructures and connected with each other. The inlet pools (4) and the positioning well (6) are connected by microchannels (3) which are formed between the microstructure layer (5) and the cover layer (1). The micro fluidic device can be applied in vitro fertilization, in determining how glial cells affect neurons, in constructing neural network and in detecting cell growth conditions.

    摘要翻译： 微流体装置包括一个微结构层（5）和一个覆盖层（1），其中覆盖层（1）连接到微结构层（5）。 微结构层（5）包括一个底层和多个微结构，用于定位样品。 覆盖层（1）包括一个顶层，一个定位孔（6）和至少一个入口池（4）。 定位井（6）位于微结构的正上方并相互连接。 入口池（4）和定位井（6）通过形成在微结构层（5）和覆盖层（1）之间的微通道（3）连接。 微流体装置可以应用于体外受精，确定神经胶质细胞如何影响神经元，构建神经网络和检测细胞生长条件。