Processes for modifying plant flowering behavior
    2.
    发明授权
    Processes for modifying plant flowering behavior 失效
    修改植物开花行为的过程

    公开(公告)号:US6025544A

    公开(公告)日:2000-02-15

    申请号:US836337

    申请日:1997-07-24

    CPC分类号: C12N15/82 C07K14/415

    摘要: Described are processes for producing plants whose flowering behavior is modified in comparison to wildtype plants, in particular for producing plants with early flower formation and flowering to an increased extent, and the plants resulting from that process. Further, the use of DNA molecules encoding sucrose carriers for modifying plant flowering behavior is described.

    摘要翻译: PCT No.PCT / EP95 / 04257 Sec。 371日期1997年7月24日 102(e)日期1997年7月24日PCT提交1995年10月30日PCT公布。 第WO96 / 13595号公报 日期1996年5月9日描述了与野生型植物相比,其开花行为被修改的植物的生产方法,特别是用于生产具有早期花形成和开花的植物,并且由该方法产生的植物。 此外,描述了编码蔗糖载体的DNA分子用于修饰植物开花行为的用途。

    Fusion proteins useful for detecting analytes
    6.
    发明申请
    Fusion proteins useful for detecting analytes 审中-公开
    用于检测分析物的融合蛋白

    公开(公告)号:US20070136825A1

    公开(公告)日:2007-06-14

    申请号:US10490153

    申请日:2002-09-13

    摘要: Disclosed are fusion proteins comprising two detection portions and a periplasmic binding protein (PBP) portion between this two detection portions which undergoes a conformational change upon binding of a compound. Also described are nucleic acid molecules comprising a nucleotide sequence encoding such a fusion protein as well as expression cassettes, vectors, transgenic plants and transgenic non-human animals comprising said nucleic acid molecules. In addition, described are methods for detecting an analyte in a sample or in a cell using said fusion protein, and optionally, a control sensor allowing a calibration of analyte detection made by said fusion protein. Also described are such control sensors and nucleic acid molecules encoding them, as well as diagnostic compositions, kits and uses of the fusion protein and the control sensor for in vitro or in vivo analyte detection and preparing a diagnostic composition.

    摘要翻译: 公开了在两个检测部分之间包含两个检测部分和周质结合蛋白(PBP)部分的融合蛋白,其在化合物结合时经历构象变化。 还描述了包含编码这种融合蛋白的核苷酸序列以及包含所述核酸分子的表达盒,载体,转基因植物和转基因非人动物的核酸分子。 此外,描述了使用所述融合蛋白检测样品或细胞中的分析物的方法,以及任选的允许校准由所述融合蛋白产生的分析物检测的控制传感器的方法。 还描述了这样的控制传感器和编码它们的核酸分子,以及用于体外或体内分析物检测和制备诊断组合物的融合蛋白和控制传感器的诊断组合物,试剂盒和用途。

    Method for producing a transgenic plant having modified transport of substances
    8.
    发明申请
    Method for producing a transgenic plant having modified transport of substances 审中-公开
    用于生产具有改变的物质运输的转基因植物的方法

    公开(公告)号:US20050235376A1

    公开(公告)日:2005-10-20

    申请号:US10514513

    申请日:2003-05-12

    摘要: The invention relates to a method for producing a transgenic plant in which the capability for incorporating undesired nitrogen compounds, especially amino acids, in the storage organs is reduced compared with the wild type, comprising the steps: introducing at least one DNA sequence and/or at least one RNA sequence corresponding to the DNA sequence and/or a mixed sequence composed of DNA and RNA nucleotides corresponding to the DNA sequence with the coding region for an amino acid transporter or parts thereof into a plant cell, wherein the DNA and/or RNA sequence and/or mixed sequence is used in sense or antisense orientation and the expression of an endogenous amino acid transporter gene is prevented or reduced, and regenerating a plant from this plant cell, wherein the DNA and/or RNA sequence is a sequence from Beta vulgaris.

    摘要翻译: 本发明涉及一种用于生产转基因植物的方法,其中与野生型相比,在储存器官中掺入不需要的氮化合物,特别是氨基酸的能力降低,包括以下步骤:引入至少一个DNA序列和/或 对应于DNA序列的至少一个RNA序列和/或由对应于具有氨基酸转运蛋白或其部分的编码区的DNA序列的DNA和RNA核苷酸组成的混合序列进入植物细胞,其中所述DNA和/或 RNA序列和/或混合序列以正义或反义方向使用,并且内源氨基酸转运蛋白基因的表达被预防或减少,并且从该植物细胞再生植物,其中所述DNA和/或RNA序列是来自 Beta寻常型

    Method for increasing the gene expression of saccharose synthase
    9.
    发明授权
    Method for increasing the gene expression of saccharose synthase 失效
    增加蔗糖合酶基因表达的方法

    公开(公告)号:US06444454B1

    公开(公告)日:2002-09-03

    申请号:US09486291

    申请日:2000-05-19

    IPC分类号: C12N900

    CPC分类号: C12N9/1062 C12N15/81

    摘要: The invention relates to a method for increasing the gene expression of saccharose synthase. To this end the invention provides for the expression of the saccharose synthase gene to be placed under the control of a proton-ATPase promoter. According to a preferred version of the invention gene expression of the saccharose synthase is also increased by increasing the number of copies of the saccharose synthase gene and of the proton-ATPase promoter.

    摘要翻译: 本发明涉及增加蔗糖合酶基因表达的方法。 为此,本发明提供将蔗糖合成酶基因置于质子-ATP酶启动子控制下的表达。 根据本发明的优选方案,蔗糖合成酶的基因表达也通过增加蔗糖合酶基因和质子-ATPase启动子的拷贝数而增加。