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公开(公告)号：US20050227262A1
公开(公告)日：2005-10-13
申请号：US11022243
申请日：2004-12-23
申请人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Chung-Tsai Lee , Ing-Er Hwang , Ching-Ping Tseng , Gwo-Fang Yuan
发明人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Chung-Tsai Lee , Ing-Er Hwang , Ching-Ping Tseng , Gwo-Fang Yuan
IPC分类号： C12N15/09 , C07H21/04 , C07K14/39 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/10 , C12N9/88 , C12N15/11 , C12N15/31 , C12N15/52 , C12N15/63 , C12N15/81 , C12P7/62 , C12P17/06 , C12Q1/68 , C12R1/645
CPC分类号： C12P7/62 , C07K14/39 , C12N15/52 , C12P17/06 , C12Y101/01088
摘要： Cholesterol inhibitor produced by Monascus, monacolin k, is a secondary metabolite of polyketides. The invention provides probes specific to monacolin k biosynthesis gene cluster. BAC clones having putative monacolin k gene cluster were screened from BAC (bacterial artificial chromosome) library, and sequencing and annotation were performed on these clones. The results show that 2 polyketide synthase (PKS) genes and 7 regulatory genes related to monacolin k synthesis were obtained. Full-length cDNAs of these genes were then obtained by RT-PCR and cloned to expression vectors for the expression of these genes.
摘要翻译： 由Monascus，monacolin k生产的胆固醇抑制剂是聚酮化合物的次级代谢产物。 本发明提供了针对莫纳可克生物合成基因簇的探针。 从BAC（细菌人造染色体）文库中筛选出具有推定的莫纳可林k基因簇的BAC克隆，对这些克隆进行测序和注释。 结果表明，获得了2个聚酮化合物合成酶（PKS）基因和7个与monacolin k合成相关的调控基因。 然后通过RT-PCR获得这些基因的全长cDNA，并克隆到用于表达这些基因的表达载体中。
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公开(公告)号：US07482157B2
公开(公告)日：2009-01-27
申请号：US11022243
申请日：2004-12-23
申请人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Chung-Tsai Lee , Ing-Er Hwang , Ching-Ping Tseng , Gwo-Fang Yuan
发明人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Chung-Tsai Lee , Ing-Er Hwang , Ching-Ping Tseng , Gwo-Fang Yuan
CPC分类号： C12P7/62 , C07K14/39 , C12N15/52 , C12P17/06 , C12Y101/01088
摘要： Cholesterol inhibitor produced by Monascus, monacolin k, is a secondary metabolite of polyketides. The invention provides probes specific to monacolin k biosynthesis gene cluster. BAC clones having putative monacolin k gene cluster were screened from BAC (bacterial artificial chromosome) library, and sequencing and annotation were performed on these clones. The results show that 2 polyketide synthase (PKS) genes and 7 regulatory genes related to monacolin k synthesis were obtained. Full-length cDNAs of these genes were then obtained by RT-PCR and cloned to expression vectors for the expression of these genes.
摘要翻译： 由Monascus，monacolin k生产的胆固醇抑制剂是聚酮化合物的次级代谢产物。 本发明提供了针对莫纳可克生物合成基因簇的探针。 从BAC（细菌人造染色体）文库中筛选出具有推定的莫纳可林k基因簇的BAC克隆，对这些克隆进行测序和注释。 结果表明，获得了2个聚酮化合物合成酶（PKS）基因和7个与monacolin k合成相关的调控基因。 然后通过RT-PCR获得这些基因的全长cDNA，并克隆到用于表达这些基因的表达载体中。
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公开(公告)号：US07465792B2
公开(公告)日：2008-12-16
申请号：US11180959
申请日：2005-07-13
申请人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Ching-Ping Tseng , Gwo-Fang Yuan
发明人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Ching-Ping Tseng , Gwo-Fang Yuan
摘要： Retrotransposons and methods related thereto. The retrotransposon comprises a nucleotide sequence selected from a group consisting of a nucleotide sequence of SEQ ID NO: 1, a nucleotide sequence of SEQ ID NO: 2, a nucleotide sequence of SEQ ID NO: 3, and a nucleotide sequence encoding a polypeptide of an amino acid sequence of SEQ ID NO: 4.
摘要翻译： 反转录转座子及其相关方法。 反转录转座子包含选自SEQ ID NO：1的核苷酸序列，SEQ ID NO：2的核苷酸序列，SEQ ID NO：3的核苷酸序列和编码SEQ ID NO：3的多肽的核苷酸序列的核苷酸序列 SEQ ID NO：4的氨基酸序列。
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公开(公告)号：US20060148088A1
公开(公告)日：2006-07-06
申请号：US11180959
申请日：2005-07-13
申请人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Ching-Ping Tseng , Gwo-Fang Yuan
发明人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Ching-Ping Tseng , Gwo-Fang Yuan
摘要： Retrotransposons and methods related thereto. The retrotransposon comprises a nucleotide sequence selected from a group consisting of a nucleotide sequence of SEQ ID NO: 1, a nucleotide sequence of SEQ ID NO: 2, a nucleotide sequence of SEQ ID NO: 3, and a nucleotide sequence encoding a polypeptide of an amino acid sequence of SEQ ID NO: 4.
摘要翻译： 反转录转座子及其相关方法。 反转录转座子包含选自SEQ ID NO：1的核苷酸序列，SEQ ID NO：2的核苷酸序列，SEQ ID NO：3的核苷酸序列和编码SEQ ID NO：3的多肽的核苷酸序列的核苷酸序列 SEQ ID NO：4的氨基酸序列。
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5.发明申请公开(公告)号：US20120295806A1
公开(公告)日：2012-11-22
申请号：US13475409
申请日：2012-05-18
申请人： Ching-Ping Tseng , Shy-Shin Chang
发明人： Ching-Ping Tseng , Shy-Shin Chang
CPC分类号： C12Q1/6848 , C12Q2525/155 , C12Q2525/161 , C12Q2549/119
摘要： This invention provides methods, systems, and compositions for detecting low abundance microbial DNA in a sample by broad-range PCR. Methods of the present invention are based on a novel strategy that tags the 5′-end of the target DNA templates with a non-bacterial tagging sequence so as to set the templates apart from the endogenous contaminants present in the PCR reagents. There is also provided fusion probes for tagging the templates and primer sets to amplify the tagged templates. Systems and kits for facilitating and automating methods of the present invention are also provided.
摘要翻译： 本发明提供了通过宽范围PCR检测样品中低丰度微生物DNA的方法，系统和组合物。 本发明的方法是基于用非细菌标签序列标记目标DNA模板的5'-端的新策略，以便将模板与PCR试剂中存在的内源性污染物区分开。 还提供了用于标记模板和引物组的融合探针以扩增标记的模板。 还提供了用于促进和自动化本发明方法的系统和套件。
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6.发明授权公开(公告)号：US09388458B2
公开(公告)日：2016-07-12
申请号：US13475409
申请日：2012-05-18
申请人： Ching-Ping Tseng , Shy-Shin Chang
发明人： Ching-Ping Tseng , Shy-Shin Chang
CPC分类号： C12Q1/6848 , C12Q2525/155 , C12Q2525/161 , C12Q2549/119
摘要： This invention provides methods, systems, and compositions for detecting low abundance microbial DNA in a sample by PCR. Methods of the present invention are based on a strategy that tags the 5′-end of the target DNA templates with a non-bacterial tagging sequence so as to set the templates apart from the endogenous contaminants present in the PCR reagents. There is also provided fusion probes for tagging the templates and primer sets to amplify the tagged templates. Systems and kits for facilitating and automating methods of the present invention are also provided.
摘要翻译： 本发明提供了通过PCR检测样品中低丰度微生物DNA的方法，系统和组合物。 本发明的方法是基于用非细菌标记序列标记目标DNA模板的5'-端的策略，以使得模板与PCR试剂中存在的内源性污染物分开。 还提供了用于标记模板和引物组的融合探针以扩增标记的模板。 还提供了用于促进和自动化本发明方法的系统和套件。
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公开(公告)号：US20150233932A1
公开(公告)日：2015-08-20
申请号：US14182710
申请日：2014-02-18
申请人： Ching-Ping Tseng , Hung-Chih Lin
发明人： Ching-Ping Tseng , Hung-Chih Lin
IPC分类号： G01N33/574 , G01N1/40
CPC分类号： G01N33/57492 , B03C1/01 , B03C1/0332 , B03C1/0335 , B03C1/288 , B03C2201/18 , B03C2201/26 , G01N1/405 , G01N33/56972 , G01N2333/70589 , G01N2800/52
摘要： The present invention discloses a highly efficient method of isolating circulating tumor cells in a blood sample by removing leukocytes and other interfering components in a blood sample. Exemplary isolation method relies on a specially configured separation column for magnetic separation of leukocytes from circulating tumor cells. Also disclosed are systems, devices, and reagents for performing the method, as well as diagnostic methods for early cancer detection, screening, and treatment monitoring utilizing the cell isolation method.
摘要翻译： 本发明公开了通过除去血液样品中的白细胞和其他干扰成分来分离血液样品中的循环肿瘤细胞的高效方法。 示例性的分离方法依赖于专门配置的用于白细胞与循环肿瘤细胞分离的分离柱。 还公开了用于执行该方法的系统，装置和试剂，以及利用细胞分离方法的早期癌症检测，筛选和治疗监测的诊断方法。
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公开(公告)号：US20110020846A1
公开(公告)日：2011-01-27
申请号：US12460656
申请日：2009-07-22
申请人： Jau-Song Yu , Li-Ping Weng , Ling-Ling Hsieh , Chih-Ching Wu , Ying Liang , Ching-Ping Tseng , Bao-Lian Hsu , Lang-Ming Chi
发明人： Jau-Song Yu , Li-Ping Weng , Ling-Ling Hsieh , Chih-Ching Wu , Ying Liang , Ching-Ping Tseng , Bao-Lian Hsu , Lang-Ming Chi
CPC分类号： G01N33/57484 , G01N2800/18
摘要： The present invention discloses an oral cancer biomarker and an inspection method using the same. The biomarker is Mca-2 binding protein (Mac-2BP), which can be directly detected in the specimen of the body fluid of a testee, and which can realize a fast and effective clinical diagnosis of oral cancer.
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公开(公告)号：US20110021750A1
公开(公告)日：2011-01-27
申请号：US12802262
申请日：2010-06-03
申请人： Jau-Song Yu , Li-Ping Weng , Ling-Ling Hsieh , Chih-Ching Wu , Ying Liang , Ching-Ping Tseng , Bao-Lian Hsu , Lang-Ming Chi
发明人： Jau-Song Yu , Li-Ping Weng , Ling-Ling Hsieh , Chih-Ching Wu , Ying Liang , Ching-Ping Tseng , Bao-Lian Hsu , Lang-Ming Chi
IPC分类号： C07K14/435
CPC分类号： G01N33/57484 , G01N2800/18
摘要： The present invention discloses an oral cancer biomarker and an inspection method using the same. The biomarker is Mca-2 binding protein (Mac-2BP), which can be directly detected in the specimen of the body fluid of a testee, and which can realize a fast and effective clinical diagnosis of oral cancer.
摘要翻译： 本发明公开了口腔癌生物标志物及其使用的检查方法。 生物标志物是Mca-2结合蛋白（Mac-2BP），可直接检测被检者体液样本，可实现口腔癌快速有效的临床诊断。
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10.发明申请公开(公告)号：US20080227181A1
公开(公告)日：2008-09-18
申请号：US11951013
申请日：2007-12-05
申请人： Ching-Ping Tseng , Ying-Chien Chung
发明人： Ching-Ping Tseng , Ying-Chien Chung
IPC分类号： A61L9/01
CPC分类号： B01D46/0023 , B01D46/30
摘要： A waste gas treatment process using biological treatment technology including filtering waste gas to be treated, and introducing the filtered waste gas into a biological treatment system periodically and switchably from two ends of the system.
摘要翻译： 一种使用生物处理技术的废气处理方法，包括过滤要处理的废气，并将经过滤的废气从系统的两端周期性和可切换地引入生物处理系统。
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