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公开(公告)号：US20050227262A1
公开(公告)日：2005-10-13
申请号：US11022243
申请日：2004-12-23
申请人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Chung-Tsai Lee , Ing-Er Hwang , Ching-Ping Tseng , Gwo-Fang Yuan
发明人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Chung-Tsai Lee , Ing-Er Hwang , Ching-Ping Tseng , Gwo-Fang Yuan
IPC分类号： C12N15/09 , C07H21/04 , C07K14/39 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/10 , C12N9/88 , C12N15/11 , C12N15/31 , C12N15/52 , C12N15/63 , C12N15/81 , C12P7/62 , C12P17/06 , C12Q1/68 , C12R1/645
CPC分类号： C12P7/62 , C07K14/39 , C12N15/52 , C12P17/06 , C12Y101/01088
摘要： Cholesterol inhibitor produced by Monascus, monacolin k, is a secondary metabolite of polyketides. The invention provides probes specific to monacolin k biosynthesis gene cluster. BAC clones having putative monacolin k gene cluster were screened from BAC (bacterial artificial chromosome) library, and sequencing and annotation were performed on these clones. The results show that 2 polyketide synthase (PKS) genes and 7 regulatory genes related to monacolin k synthesis were obtained. Full-length cDNAs of these genes were then obtained by RT-PCR and cloned to expression vectors for the expression of these genes.
摘要翻译： 由Monascus，monacolin k生产的胆固醇抑制剂是聚酮化合物的次级代谢产物。 本发明提供了针对莫纳可克生物合成基因簇的探针。 从BAC（细菌人造染色体）文库中筛选出具有推定的莫纳可林k基因簇的BAC克隆，对这些克隆进行测序和注释。 结果表明，获得了2个聚酮化合物合成酶（PKS）基因和7个与monacolin k合成相关的调控基因。 然后通过RT-PCR获得这些基因的全长cDNA，并克隆到用于表达这些基因的表达载体中。
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公开(公告)号：US07482157B2
公开(公告)日：2009-01-27
申请号：US11022243
申请日：2004-12-23
申请人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Chung-Tsai Lee , Ing-Er Hwang , Ching-Ping Tseng , Gwo-Fang Yuan
发明人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Chung-Tsai Lee , Ing-Er Hwang , Ching-Ping Tseng , Gwo-Fang Yuan
CPC分类号： C12P7/62 , C07K14/39 , C12N15/52 , C12P17/06 , C12Y101/01088
摘要： Cholesterol inhibitor produced by Monascus, monacolin k, is a secondary metabolite of polyketides. The invention provides probes specific to monacolin k biosynthesis gene cluster. BAC clones having putative monacolin k gene cluster were screened from BAC (bacterial artificial chromosome) library, and sequencing and annotation were performed on these clones. The results show that 2 polyketide synthase (PKS) genes and 7 regulatory genes related to monacolin k synthesis were obtained. Full-length cDNAs of these genes were then obtained by RT-PCR and cloned to expression vectors for the expression of these genes.
摘要翻译： 由Monascus，monacolin k生产的胆固醇抑制剂是聚酮化合物的次级代谢产物。 本发明提供了针对莫纳可克生物合成基因簇的探针。 从BAC（细菌人造染色体）文库中筛选出具有推定的莫纳可林k基因簇的BAC克隆，对这些克隆进行测序和注释。 结果表明，获得了2个聚酮化合物合成酶（PKS）基因和7个与monacolin k合成相关的调控基因。 然后通过RT-PCR获得这些基因的全长cDNA，并克隆到用于表达这些基因的表达载体中。
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公开(公告)号：US07465792B2
公开(公告)日：2008-12-16
申请号：US11180959
申请日：2005-07-13
申请人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Ching-Ping Tseng , Gwo-Fang Yuan
发明人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Ching-Ping Tseng , Gwo-Fang Yuan
摘要： Retrotransposons and methods related thereto. The retrotransposon comprises a nucleotide sequence selected from a group consisting of a nucleotide sequence of SEQ ID NO: 1, a nucleotide sequence of SEQ ID NO: 2, a nucleotide sequence of SEQ ID NO: 3, and a nucleotide sequence encoding a polypeptide of an amino acid sequence of SEQ ID NO: 4.
摘要翻译： 反转录转座子及其相关方法。 反转录转座子包含选自SEQ ID NO：1的核苷酸序列，SEQ ID NO：2的核苷酸序列，SEQ ID NO：3的核苷酸序列和编码SEQ ID NO：3的多肽的核苷酸序列的核苷酸序列 SEQ ID NO：4的氨基酸序列。
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公开(公告)号：US20060148088A1
公开(公告)日：2006-07-06
申请号：US11180959
申请日：2005-07-13
申请人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Ching-Ping Tseng , Gwo-Fang Yuan
发明人： Yi-Pei Chen , Li-Ling Liaw , Chun-Lin Wang , Ching-Ping Tseng , Gwo-Fang Yuan
摘要： Retrotransposons and methods related thereto. The retrotransposon comprises a nucleotide sequence selected from a group consisting of a nucleotide sequence of SEQ ID NO: 1, a nucleotide sequence of SEQ ID NO: 2, a nucleotide sequence of SEQ ID NO: 3, and a nucleotide sequence encoding a polypeptide of an amino acid sequence of SEQ ID NO: 4.
摘要翻译： 反转录转座子及其相关方法。 反转录转座子包含选自SEQ ID NO：1的核苷酸序列，SEQ ID NO：2的核苷酸序列，SEQ ID NO：3的核苷酸序列和编码SEQ ID NO：3的多肽的核苷酸序列的核苷酸序列 SEQ ID NO：4的氨基酸序列。
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公开(公告)号：US08114665B2
公开(公告)日：2012-02-14
申请号：US12251056
申请日：2008-10-14
申请人： Wen-Jung Wu , Chung-Tsai Lee , Jyh-Wei Chen , Hasiao Chi Peng , Li-Ling Liaw , Gwo-Fang Yuan
发明人： Wen-Jung Wu , Chung-Tsai Lee , Jyh-Wei Chen , Hasiao Chi Peng , Li-Ling Liaw , Gwo-Fang Yuan
IPC分类号： C12N15/00
摘要： Promoters and usage thereof. Two promoters, acuF and hsp promoters, comprise the nucleotide sequences of SEQ ID NO: 1 and 2, respectively.
摘要翻译： 促销者及其用途。 两个启动子，acuF和hsp启动子分别包含SEQ ID NO：1和2的核苷酸序列。
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公开(公告)号：US20050164369A1
公开(公告)日：2005-07-28
申请号：US11008728
申请日：2004-12-09
申请人： I-Ching Chen , En-Hui Kuo , Yi-Ju Liu , Ing-Er Hwang , Wen-Jung Wu , Chung-Tsai Lee , Jyh-Wei Chen , Hasiao Peng , Li-Ling Liaw , Gwo-Fang Yuan
发明人： I-Ching Chen , En-Hui Kuo , Yi-Ju Liu , Ing-Er Hwang , Wen-Jung Wu , Chung-Tsai Lee , Jyh-Wei Chen , Hasiao Peng , Li-Ling Liaw , Gwo-Fang Yuan
IPC分类号： C12N15/09 , C07K14/37 , C12N1/16 , C12N9/88 , C12N15/31 , C12N15/63 , C12N15/67 , C12N15/74 , C12N15/80
摘要： Promoters and usage thereof. Two promoters, acuF and hsp promoters, comprise the nucleotide sequences of SEQ ID NO: 1 and 2, respectively.
摘要翻译： 促销者及其用途。 两种启动子，acuF和hsp启动子分别包含SEQ ID NO：1和2的核苷酸序列。
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公开(公告)号：US07534602B2
公开(公告)日：2009-05-19
申请号：US11008728
申请日：2004-12-09
申请人： I-Ching Chen , En-Hui Kuo , Yi-Ju Liu , Ing-Er Hwang , Li-Ling Liaw , Gwo-Fang Yuan
发明人： I-Ching Chen , En-Hui Kuo , Yi-Ju Liu , Ing-Er Hwang , Li-Ling Liaw , Gwo-Fang Yuan
IPC分类号： C12N15/00
摘要： Promoters and usage thereof. Two promoters, acuF and hsp promoters, comprise the nucleotide sequences of SEQ ID NO: 1 and 2, respectively.
摘要翻译： 促销者及其用途。 两个启动子，acuF和hsp启动子分别包含SEQ ID NO：1和2的核苷酸序列。
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公开(公告)号：US07790188B2
公开(公告)日：2010-09-07
申请号：US11646352
申请日：2006-12-27
申请人： Ching-Yu Tu , Li-Ling Liaw
发明人： Ching-Yu Tu , Li-Ling Liaw
CPC分类号： C12N15/8282 , A01N63/04 , A61K38/00 , C07K14/37
摘要： The present invention relates to an antifungal protein gene and cDNA sequence thereof, which is obtained by mining the whole genome sequences of Monascus pilosus BCRC 38072 and the unigene database. The gene can encode an antifungal protein MAFP1. A purified protein obtained from M. pilosus culture broth having molecular weight of about 7 kDa is identified as MAFP1 by N-terminal protein sequencing and comparative analysis. The purified MAFP1 protein can inhibit the growth of pathogens such as Paecilomyces variotii BCRC 33174 and Helminthosporium panici BCRC 35004. In addition, it is found by PCR test that the gene of this antifungal protein exists in other Monascus species such as M. Barkeri, M. floridanus, M. lunisporas, M. pilosus, M. ruber and the like. It is also been proved that the mafp1 gene and cDNA thereof in four Monascus strains, M. pilosus (BCRC 38072, BCRC 38093 and BCRC 31502) and M. ruber BCRC 31533, have the same DNA sequences.
摘要翻译： 本发明涉及通过开发红曲霉BCRC 38072的全基因组序列和单基因数据库获得的抗真菌蛋白质基因及其cDNA序列。 该基因可编码抗真菌蛋白MAFP1。 通过N末端蛋白质测序和比较分析，将得自具有约7kDa分子量的M. pilosus培养液的纯化蛋白质鉴定为MAFP1。 纯化的MAFP1蛋白可以抑制病原体的生长，如拟青霉BCPC 33174和Helminthosporium panici BCRC 35004.另外，通过PCR检测发现，该抗真菌蛋白的基因存在于其他红曲霉属物种如M.Barkeri，M 花椒（M.orisporas），马尾松属（M. pilosus），木霉属（M.ruber）等。 也证明了四种红曲霉菌（M.closlosus）（BCRC 38072，BCRC 38093和BCRC 31502）和M.ruber BCRC 31533中的mafp1基因及其cDNA具有相同的DNA序列。
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公开(公告)号：US20080161225A1
公开(公告)日：2008-07-03
申请号：US11646352
申请日：2006-12-27
申请人： Ching-Yu Tu , Li-Ling Liaw
发明人： Ching-Yu Tu , Li-Ling Liaw
IPC分类号： A01H5/00 , A01N37/18 , A23J1/18 , A61K38/00 , A61P43/00 , C07H21/04 , C12N15/00 , C12N5/04 , C12Q1/68
CPC分类号： C12N15/8282 , A01N63/04 , A61K38/00 , C07K14/37
摘要： The present invention relates to an antifungal protein gene and cDNA sequence thereof, which is obtained by mining the whole genome sequences of Monascus pilosus BCRC 38072 and the unigene database. The gene can encode an antifungal protein MAFP1. A purified protein obtained from M. pilosus culture broth having molecular weight of about 7 kDa is identified as MAFP1 by N-terminal protein sequencing and comparative analysis. The purified MAFP1 protein can inhibit the growth of pathogens such as Paecilomyces variotii BCRC 33174 and Helminthosporium panici BCRC 35004. In addition, it is found by PCR test that the gene of this antifungal protein exists in other Monascus species such as M. Barkeri, M. floridanus, M. lunisporas, M. pilosus, M. ruber and the like. It is also been proved that the mafp1 gene and cDNA thereof in four Monascus strains, M. pilosus (BCRC 38072, BCRC 38093 and BCRC 31502) and M. ruber BCRC 31533, have the same DNA sequences.
摘要翻译： 本发明涉及通过开发红曲霉BCRC 38072的全基因组序列和单基因数据库获得的抗真菌蛋白质基因及其cDNA序列。 该基因可编码抗真菌蛋白MAFP1。 通过N末端蛋白质测序和比较分析，将得自具有约7kDa分子量的M. pilosus培养液的纯化蛋白质鉴定为MAFP1。 纯化的MAFP1蛋白可以抑制病原体的生长，如拟青霉BCPC 33174和Helminthosporium panici BCRC 35004.另外，通过PCR检测发现，该抗真菌蛋白的基因存在于其他红曲霉属物种如M.Barkeri，M 花椒（M.orisporas），马尾松属（M. pilosus），木霉属（M.ruber）等。 也证明了四种红曲霉菌（M.closlosus）（BCRC 38072，BCRC 38093和BCRC 31502）和M.ruber BCRC 31533中的mafp1基因及其cDNA具有相同的DNA序列。
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10.发明授权β-agarase isolated from Thalassomonas agarivorans, preparation process and uses thereof 有权从琼脂糖培养基中分离的β-琼脂糖酶，其制备方法和用途公开(公告)号：US07662606B1
公开(公告)日：2010-02-16
申请号：US12243714
申请日：2008-10-01
申请人： Shu-Shan Liang , Yi-Hong Chen , Li-Ling Liaw
发明人： Shu-Shan Liang , Yi-Hong Chen , Li-Ling Liaw
CPC分类号： C12P19/04 , C12N9/2434 , C12Y302/01081 , Y10S435/81 , Y10S435/975
摘要： The present invention relates to the cloning and expression of a novel β-agarase gene, agaB1, and a novel β-agarase, AgaB1, encoded from the gene. The present invention also provides a method for producing the β-agarase, and the utilization of the β-agarase.
摘要翻译： 本发明涉及从基因编码的新型β-琼脂糖酶基因，agaB1和新型β-琼脂糖酶AgaB1的克隆和表达。 本发明还提供了β-琼脂糖酶的生产方法和β-琼脂糖酶的利用。
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