公开(公告)号：US6001626A

公开(公告)日：1999-12-14

申请号：US982312

申请日：1997-12-01

申请人： Yoshitsugu Kosugi ,  Katsuhiko Higuchi ,  Kazuhiko Ishikawa ,  Ikuo Matsui ,  Joh Yong-Goe

发明人： Yoshitsugu Kosugi ,  Katsuhiko Higuchi ,  Kazuhiko Ishikawa ,  Ikuo Matsui ,  Joh Yong-Goe

IPC分类号： C12N9/18

CPC分类号： C12Y301/01032 ,  C12N9/18 ,  C12Y301/01004

摘要： Disclosed are a thermophilic phospholipase having an optimum temperature range of 95 to 105.degree. C. which is useful in high-temperature degumming processes in oil refining process and high-temperature processing of phospholipids; and a method for producing a thermophilic phospholipase comprising culturing a microorganism capable of producing the phospholipase in a culture medium and collecting the phospholipase from the resultant culture.

摘要翻译： 公开了具有95至105℃的最佳温度范围的嗜热磷脂酶，其可用于炼油过程中的高温脱胶过程和磷脂的高温加工; 以及生产嗜热磷脂酶的方法，包括在培养基中培养能够产生磷脂酶的微生物并从所得培养物中收集磷脂酶。

公开(公告)号：US6133012A

公开(公告)日：2000-10-17

申请号：US16080

申请日：1998-01-30

申请人： Kazuhiko Ishikawa ,  Ikuo Matsui ,  Hiroyasu Ishida ,  Yoshitsugu Kosugi ,  Katsuhiko Higuchi

发明人： Kazuhiko Ishikawa ,  Ikuo Matsui ,  Hiroyasu Ishida ,  Yoshitsugu Kosugi ,  Katsuhiko Higuchi

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N9/48 ,  C12N9/78 ,  C12N15/00 ,  C12Q1/37 ,  C12R1/01 ,  C12R1/19 ,  C12N9/52

CPC分类号： C12N9/48

摘要： An acyl peptide hydrolase having an optimum temperature range of 90-95.degree. C. and a gene encoding the same are disclosed. With the above enzyme, it becomes possible to conduct amino terminal analysis of acylated proteins and peptides at high temperatures.

摘要翻译： 公开了具有最佳温度范围为90-95℃的酰基肽水解酶和编码该酰基肽水解酶的基因。 利用上述酶，可以在高温下进行酰化蛋白质和肽的氨基末端分析。

公开(公告)号：US06960454B2

公开(公告)日：2005-11-01

申请号：US09369735

申请日：1999-08-06

申请人： Ikuo Matsui ,  Kazuhiko Ishikawa ,  Hiroyasu Ishida ,  Yoshitsugu Kosugi

发明人： Ikuo Matsui ,  Kazuhiko Ishikawa ,  Hiroyasu Ishida ,  Yoshitsugu Kosugi

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/24 ,  C12N9/42 ,  C12N15/00 ,  C12R1/01 ,  C12R1/19 ,  C12P19/04 ,  C07K1/00 ,  C12N9/26 ,  C12P19/02

CPC分类号： C12Y302/01021 ,  C12N9/2402

摘要： The invention relates to a thermophilic enzyme having β-glycosidase activity which comprises the amino acid sequence of SEQ ID NO: 2 in which one or a plurality of amino acid residues may be deleted, replaced or added.

摘要翻译： 本发明涉及具有β-糖苷酶活性的嗜热酶，其包含SEQ ID NO：2的氨基酸序列，其中可以缺失，替换或添加一个或多个氨基酸残基。

公开(公告)号：US06391604B1

公开(公告)日：2002-05-21

申请号：US09519729

申请日：2000-03-03

申请人： Ikuo Matsui ,  Kazuhiko Ishikawa ,  Hiroyasu Ishida ,  Yoshitsugu Kosugi ,  Yasutaka Tahara

发明人： Ikuo Matsui ,  Kazuhiko Ishikawa ,  Hiroyasu Ishida ,  Yoshitsugu Kosugi ,  Yasutaka Tahara

IPC分类号： C12N910

CPC分类号： C12N9/1007 ,  C12Y201/01017 ,  Y10S435/822

摘要： A thermostable phosphatidylethanolamine N-methyltransferase and a process for producing the enzyme are provided. A host cell is transformed with an expression vector containing DNA encoding a thermostable enzyme derived from hyper-thermophilic archaea such as Pyrococcus, the enzyme having phosphatidylethanolamine N-methyltransferase activity and an optimum temperature of 90° C. or higher, and the transformed host cell is cultured.

摘要翻译： 提供了热稳定性磷脂酰乙醇胺N-甲基转移酶及其制备方法。 宿主细胞用含有编码来源于嗜热古细菌的热稳定酶的DNA的表达载体转化，如Pyrococcus，具有磷脂酰乙醇胺N-甲基转移酶活性的酶，最适温度为90℃或更高，转化的宿主细胞 被培养

公开(公告)号：US06255081B1

公开(公告)日：2001-07-03

申请号：US09175973

申请日：1998-10-21

申请人： Ikuo Matsui ,  Kazuhiko Ishikawa ,  Yoshitsugu Kosugi ,  Eriko Matsui ,  Satoko Kawasaki

发明人： Ikuo Matsui ,  Kazuhiko Ishikawa ,  Yoshitsugu Kosugi ,  Eriko Matsui ,  Satoko Kawasaki

IPC分类号： C12P1934

CPC分类号： C12N9/22

摘要： The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75° C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.

摘要翻译： 本发明涉及最适温度为75℃或更高的热稳定翼片内切核酸酶和编码（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质的DNA，或（b）具有瓣内切核酸酶活性的蛋白质 由氨基酸序列（a），一个或多个氨基酸缺失，取代或添加的氨基酸序列组成。 根据本发明，提供了其最佳反应温度为75℃或更高的热稳定翼片内切核酸酶。 此外，该酶是热稳定的，因此可以开发通过将酶反应与PCR偶联来高效地进行人工同源重组或遗传改组的新技术

公开(公告)号：US06251649B1

公开(公告)日：2001-06-26

申请号：US09146319

申请日：1998-09-03

申请人： Ikuo Matsui ,  Kazuhiko Ishikawa ,  Yoshitsugu Kosugi ,  Eriko Matsui ,  Satoko Kawasaki

发明人： Ikuo Matsui ,  Kazuhiko Ishikawa ,  Yoshitsugu Kosugi ,  Eriko Matsui ,  Satoko Kawasaki

IPC分类号： C12N922

CPC分类号： C07K14/195 ,  C12N9/22

摘要： The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75° C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.

摘要翻译： 本发明涉及最适温度为75℃或更高的热稳定翼片内切核酸酶和编码（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质的DNA，或（b）具有瓣内切核酸酶活性的蛋白质 由氨基酸序列（a），一个或多个氨基酸缺失，取代或添加的氨基酸序列组成。 根据本发明，提供了其最佳反应温度为75℃或更高的热稳定翼片内切核酸酶。 此外，该酶是热稳定的，因此可以开发通过将酶反应与PCR偶联来高效地进行人工同源重组或遗传改组的新技术

公开(公告)号：US5635378A

公开(公告)日：1997-06-03

申请号：US467831

申请日：1995-06-06

申请人： Ikuo Matsui ,  Kazuhiko Ishikawa ,  Sachio Miyairi ,  Koichi Honda

发明人： Ikuo Matsui ,  Kazuhiko Ishikawa ,  Sachio Miyairi ,  Koichi Honda

IPC分类号： C12N9/10 ,  C12N9/30 ,  C12N15/09 ,  C12N15/54 ,  C12P19/14 ,  C12P19/18 ,  C12R1/07 ,  C12R1/125 ,  C12R1/645 ,  C12R1/865 ,  C12P19/00

CPC分类号： C12N9/242 ,  C12N9/1074 ,  C12P19/18 ,  Y10S435/832

摘要： There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.

公开(公告)号：US5631149A

公开(公告)日：1997-05-20

申请号：US470702

申请日：1995-06-06

申请人： Ikuo Matsui ,  Kazuhiko Ishikawa ,  Sachio Miyairi ,  Koichi Honda

发明人： Ikuo Matsui ,  Kazuhiko Ishikawa ,  Sachio Miyairi ,  Koichi Honda

IPC分类号： C12N9/10 ,  C12N9/30 ,  C12N15/09 ,  C12N15/54 ,  C12P19/14 ,  C12P19/18 ,  C12R1/07 ,  C12R1/125 ,  C12R1/645 ,  C12R1/865 ,  C12P19/04

CPC分类号： C12N9/242 ,  C12N9/1074 ,  C12P19/18 ,  Y10S435/832

摘要： There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.

摘要翻译： 公开了通过用另一个氨基酸残基取代存在于水解酶的活性中心的酪氨酸残基而已经增加转糖基化活性的变体型碳水化合物水解酶，该水解酶是淀粉酶或类似于淀粉酶的酶; 具有导入编码酪氨酸残基的碱基序列的突变的碳水化合物水解酶的基因或DNA序列; 以及包含DNA序列的载体或转化体。 还公开了通过使用变体型碳水化合物水解酶来生产各种寡糖等的方法。

公开(公告)号：US5538882A

公开(公告)日：1996-07-23

申请号：US204656

申请日：1994-03-02

申请人： Ikuo Matsui ,  Kazuhiko Ishikawa ,  Sachio Miyairi ,  Koichi Honda

发明人： Ikuo Matsui ,  Kazuhiko Ishikawa ,  Sachio Miyairi ,  Koichi Honda

IPC分类号： C12N9/10 ,  C12N9/30 ,  C12N15/09 ,  C12N15/54 ,  C12P19/14 ,  C12P19/18 ,  C12R1/07 ,  C12R1/125 ,  C12R1/645 ,  C12R1/865 ,  C12N15/56

CPC分类号： C12N9/242 ,  C12N9/1074 ,  C12P19/18 ,  Y10S435/832

摘要： There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.

摘要翻译： 公开了通过用另一个氨基酸残基取代存在于水解酶的活性中心的酪氨酸残基而已经增加转糖基化活性的变体型碳水化合物水解酶，该水解酶是淀粉酶或类似于淀粉酶的酶; 具有导入编码酪氨酸残基的碱基序列的突变的碳水化合物水解酶的基因或DNA序列; 以及包含DNA序列的载体或转化体。 还公开了通过使用变体型碳水化合物水解酶来生产各种寡糖等的方法。

公开(公告)号：US07824886B2

公开(公告)日：2010-11-02

申请号：US11748961

申请日：2007-05-15

申请人： Ikuo Matsui ,  Yuji Urushibata

发明人： Ikuo Matsui ,  Yuji Urushibata

IPC分类号： C12P19/34 ,  A61K38/16

CPC分类号： C12N9/22 ,  C12P19/34

摘要： A DNA polymerase reaction system which provides high DNA polymerase activity even at a high temperature and at a high salt concentration. A DNA polymerase reaction system that is constructed from a DNA polymerase, a clamp, and a clamp loader without intein sequence, the DNA polymerase being from Pyrococcus horikoshii, a hyperthermophilic archaeon.

摘要翻译： DNA聚合酶反应体系即使在高温和高盐浓度下也能提供高DNA聚合酶活性。 由DNA聚合酶，钳夹和没有内含肽序列的钳夹装置构建的DNA聚合酶反应系统，DNA聚合酶来自热球菌Pyococcus horikoshii，一种超嗜热古细菌。