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公开(公告)号：US6133012A
公开(公告)日：2000-10-17
申请号：US16080
申请日：1998-01-30
IPC分类号： C12N15/09 , C07H21/04 , C12N9/48 , C12N9/78 , C12N15/00 , C12Q1/37 , C12R1/01 , C12R1/19 , C12N9/52
CPC分类号： C12N9/48
摘要： An acyl peptide hydrolase having an optimum temperature range of 90-95.degree. C. and a gene encoding the same are disclosed. With the above enzyme, it becomes possible to conduct amino terminal analysis of acylated proteins and peptides at high temperatures.
摘要翻译： 公开了具有最佳温度范围为90-95℃的酰基肽水解酶和编码该酰基肽水解酶的基因。 利用上述酶，可以在高温下进行酰化蛋白质和肽的氨基末端分析。
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公开(公告)号：US6001626A
公开(公告)日：1999-12-14
申请号：US982312
申请日：1997-12-01
IPC分类号： C12N9/18
CPC分类号： C12Y301/01032 , C12N9/18 , C12Y301/01004
摘要： Disclosed are a thermophilic phospholipase having an optimum temperature range of 95 to 105.degree. C. which is useful in high-temperature degumming processes in oil refining process and high-temperature processing of phospholipids; and a method for producing a thermophilic phospholipase comprising culturing a microorganism capable of producing the phospholipase in a culture medium and collecting the phospholipase from the resultant culture.
摘要翻译： 公开了具有95至105℃的最佳温度范围的嗜热磷脂酶，其可用于炼油过程中的高温脱胶过程和磷脂的高温加工; 以及生产嗜热磷脂酶的方法，包括在培养基中培养能够产生磷脂酶的微生物并从所得培养物中收集磷脂酶。
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公开(公告)号：US06960454B2
公开(公告)日：2005-11-01
申请号：US09369735
申请日：1999-08-06
IPC分类号： C12N15/09 , C12N1/21 , C12N9/24 , C12N9/42 , C12N15/00 , C12R1/01 , C12R1/19 , C12P19/04 , C07K1/00 , C12N9/26 , C12P19/02
CPC分类号： C12Y302/01021 , C12N9/2402
摘要： The invention relates to a thermophilic enzyme having β-glycosidase activity which comprises the amino acid sequence of SEQ ID NO: 2 in which one or a plurality of amino acid residues may be deleted, replaced or added.
摘要翻译： 本发明涉及具有β-糖苷酶活性的嗜热酶，其包含SEQ ID NO：2的氨基酸序列，其中可以缺失，替换或添加一个或多个氨基酸残基。
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4.发明授权Thermostable enzyme having phosphatidylethanolamine N-methyltransferase activity 失效具有磷脂酰乙醇胺N-甲基转移酶活性的热稳定酶公开(公告)号：US06391604B1
公开(公告)日：2002-05-21
申请号：US09519729
申请日：2000-03-03
IPC分类号： C12N910
CPC分类号： C12N9/1007 , C12Y201/01017 , Y10S435/822
摘要： A thermostable phosphatidylethanolamine N-methyltransferase and a process for producing the enzyme are provided. A host cell is transformed with an expression vector containing DNA encoding a thermostable enzyme derived from hyper-thermophilic archaea such as Pyrococcus, the enzyme having phosphatidylethanolamine N-methyltransferase activity and an optimum temperature of 90° C. or higher, and the transformed host cell is cultured.
摘要翻译： 提供了热稳定性磷脂酰乙醇胺N-甲基转移酶及其制备方法。 宿主细胞用含有编码来源于嗜热古细菌的热稳定酶的DNA的表达载体转化，如Pyrococcus，具有磷脂酰乙醇胺N-甲基转移酶活性的酶，最适温度为90℃或更高，转化的宿主细胞 被培养
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5.发明授权Thermostable flap endonuclease derived from hyperthermophile bacterium belonging to the genus pyrococcus 失效衍生自属于高球菌属的嗜热菌细菌的热稳定性瓣内切酶公开(公告)号：US06255081B1
公开(公告)日：2001-07-03
申请号：US09175973
申请日：1998-10-21
IPC分类号： C12P1934
CPC分类号： C12N9/22
摘要： The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75° C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.
摘要翻译： 本发明涉及最适温度为75℃或更高的热稳定翼片内切核酸酶和编码（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质的DNA，或（b）具有瓣内切核酸酶活性的蛋白质 由氨基酸序列（a），一个或多个氨基酸缺失，取代或添加的氨基酸序列组成。 根据本发明，提供了其最佳反应温度为75℃或更高的热稳定翼片内切核酸酶。 此外，该酶是热稳定的，因此可以开发通过将酶反应与PCR偶联来高效地进行人工同源重组或遗传改组的新技术
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6.发明授权Thermostable flap endonuclease derived from a hyperthermophile bacterium belonging to the genus pyrococcus 失效衍生自属于高球菌属的嗜热菌细菌的热稳定性瓣内切酶公开(公告)号：US06251649B1
公开(公告)日：2001-06-26
申请号：US09146319
申请日：1998-09-03
IPC分类号： C12N922
CPC分类号： C07K14/195 , C12N9/22
摘要： The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75° C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.
摘要翻译： 本发明涉及最适温度为75℃或更高的热稳定翼片内切核酸酶和编码（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质的DNA，或（b）具有瓣内切核酸酶活性的蛋白质 由氨基酸序列（a），一个或多个氨基酸缺失，取代或添加的氨基酸序列组成。 根据本发明，提供了其最佳反应温度为75℃或更高的热稳定翼片内切核酸酶。 此外，该酶是热稳定的，因此可以开发通过将酶反应与PCR偶联来高效地进行人工同源重组或遗传改组的新技术
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7.发明授权Variant-type carbohydrate hydrolase, variant gene of the enzyme and method for producing oligosaccharide using the enzyme 失效变体型碳水化合物水解酶，酶的变体基因和使用该酶生产寡糖的方法
公开(公告)号：US5538882A
公开(公告)日：1996-07-23
申请号：US204656
申请日：1994-03-02
申请人： Ikuo Matsui , Kazuhiko Ishikawa , Sachio Miyairi , Koichi Honda
发明人： Ikuo Matsui , Kazuhiko Ishikawa , Sachio Miyairi , Koichi Honda
IPC分类号： C12N9/10 , C12N9/30 , C12N15/09 , C12N15/54 , C12P19/14 , C12P19/18 , C12R1/07 , C12R1/125 , C12R1/645 , C12R1/865 , C12N15/56
CPC分类号： C12N9/242 , C12N9/1074 , C12P19/18 , Y10S435/832
摘要： There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.
摘要翻译： 公开了通过用另一个氨基酸残基取代存在于水解酶的活性中心的酪氨酸残基而已经增加转糖基化活性的变体型碳水化合物水解酶，该水解酶是淀粉酶或类似于淀粉酶的酶; 具有导入编码酪氨酸残基的碱基序列的突变的碳水化合物水解酶的基因或DNA序列; 以及包含DNA序列的载体或转化体。 还公开了通过使用变体型碳水化合物水解酶来生产各种寡糖等的方法。
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公开(公告)号：US5635378A
公开(公告)日：1997-06-03
申请号：US467831
申请日：1995-06-06
申请人： Ikuo Matsui , Kazuhiko Ishikawa , Sachio Miyairi , Koichi Honda
发明人： Ikuo Matsui , Kazuhiko Ishikawa , Sachio Miyairi , Koichi Honda
IPC分类号： C12N9/10 , C12N9/30 , C12N15/09 , C12N15/54 , C12P19/14 , C12P19/18 , C12R1/07 , C12R1/125 , C12R1/645 , C12R1/865 , C12P19/00
CPC分类号： C12N9/242 , C12N9/1074 , C12P19/18 , Y10S435/832
摘要： There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.
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9.发明授权Variant-type carbohydrate hydrolase, variant gene of the enzyme and method for producing oligosaccharide using the enzyme 失效变体型碳水化合物水解酶，酶的变体基因和使用该酶生产寡糖的方法
公开(公告)号：US5631149A
公开(公告)日：1997-05-20
申请号：US470702
申请日：1995-06-06
申请人： Ikuo Matsui , Kazuhiko Ishikawa , Sachio Miyairi , Koichi Honda
发明人： Ikuo Matsui , Kazuhiko Ishikawa , Sachio Miyairi , Koichi Honda
IPC分类号： C12N9/10 , C12N9/30 , C12N15/09 , C12N15/54 , C12P19/14 , C12P19/18 , C12R1/07 , C12R1/125 , C12R1/645 , C12R1/865 , C12P19/04
CPC分类号： C12N9/242 , C12N9/1074 , C12P19/18 , Y10S435/832
摘要： There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.
摘要翻译： 公开了通过用另一个氨基酸残基取代存在于水解酶的活性中心的酪氨酸残基而已经增加转糖基化活性的变体型碳水化合物水解酶，该水解酶是淀粉酶或类似于淀粉酶的酶; 具有导入编码酪氨酸残基的碱基序列的突变的碳水化合物水解酶的基因或DNA序列; 以及包含DNA序列的载体或转化体。 还公开了通过使用变体型碳水化合物水解酶来生产各种寡糖等的方法。
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10.发明授权Modification of xylanase to improve thermophilicity, alkophilicity and thermostability 失效修饰木聚糖酶以改善嗜热性，亲油性和热稳定性
公开(公告)号：US5866408A
公开(公告)日：1999-02-02
申请号：US47370
申请日：1998-03-25
申请人： Wing L. Sung , Makoto Yaguchi , Kazuhiko Ishikawa
发明人： Wing L. Sung , Makoto Yaguchi , Kazuhiko Ishikawa
IPC分类号： C12N15/09 , C12N9/24 , C12N15/00 , C12N15/56 , C12R1/09 , C12R1/885 , D21C5/00 , D21C9/10 , D21C3/00
CPC分类号： C12Y302/01032 , C12N9/248 , C12Y302/01008 , D21C5/005 , C07K2319/00
摘要： Producing a xylanase enzyme of superior performance in the bleaching of pulp. More specifically, a modified xylanase of Family 11 that shows improved thermophilicity, alkalophilicity, and thermostability as compared to the natural xylanase. The modified xylanases contain any of three types of modifications: (1) changing amino acids 10, 27, and 29 of Trichoderma reesei xylanase II or the corresponding amino acids of another Family 11 xylanase, where these amino acids are changed to histidine, methionine, and leucine, respectively; (2) substitution of amino acids in the N-terminal region with amino acids from another xylanase enzyme. In a preferred embodiment, substitution of the natural Bacillus circulans or Trichoderma reesei xylanase with a short sequence of amino acids from Thermomonospora fusca xylanase yielded chimeric xylanases with higher thermophilicity and alkalophilicity; (3) an extension upstream of the N-terminus of up to 10 amino acids. In a preferred embodiment, extension of the N-terminus of the xylanase with the tripeptide glycine-arginine-arginine improved its performance.
摘要翻译： 在木浆漂白中生产出优异的木聚糖酶。 更具体地，与天然木聚糖酶相比，家族11的改性木聚糖酶显示出改善的嗜热性，嗜碱性和热稳定性。 修饰的木聚糖酶包含三种类型的修饰中的任一种：（1）改变里氏木霉木聚糖酶II的氨基酸10,27和29或另一种家族11木聚糖酶的相应氨基酸，其中这些氨基酸被改变为组氨酸，甲硫氨酸， 和亮氨酸; （2）用来自另一种木聚糖酶的氨基酸取代N-末端区域中的氨基酸。 在一个优选的实施方案中，用热单孢菌木聚糖酶短链氨基酸取代天然环状芽孢杆菌或里氏木霉木聚糖酶得到具有较高嗜热性和嗜碱性的嵌合木聚糖酶; （3）N-末端上游至多10个氨基酸的延伸。 在优选的实施方案中，木聚糖酶的N-末端与三肽甘氨酸 - 精氨酸 - 精氨酸的延伸改善了其性能。
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