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    MULTIPLE COMPARTMENT DOSING MODEL
    6.
    发明申请
    MULTIPLE COMPARTMENT DOSING MODEL 有权
    多个舱室配料模型

    公开(公告)号：US20100106473A1

    公开(公告)日：2010-04-29

    申请号：US12604855

    申请日：2009-10-23

    申请人： Daniel J. Lockhart ,  David J. Lockhart

    发明人： Daniel J. Lockhart ,  David J. Lockhart

    IPC分类号： G06F17/10

    CPC分类号： G06F19/12

    摘要： The method for modeling a dosing regimen for a medicament includes providing a system having at least two biocompartments. The method determines steady state levels in each of the biocompartments. After that, the method provides for modifying values to calculate an amount of at least one protein in one of the biocompartments to which the protein is being transported. Next, the method calculates and integrates a weighted value of the protein to find a weighted area under the curve. Then, the method calculates and integrates a non-drug value of the protein to find a non-drug area under the curve. Finally, the method evaluates one or more dosing regimens by comparing the weighted area under the curve and the non-drug area under the curve of the protein to determine a net effect of the drug over the time period.

    摘要翻译： 用于建模用于药物的给药方案的方法包括提供具有至少两个生物隔离物的系统。 该方法确定每个生物隔离物中的稳态水平。 之后，该方法提供修饰值以计算蛋白质正在运输到的一个生物隔离物中的至少一种蛋白质的量。 接下来，该方法计算并积分蛋白质的加权值以找到曲线下的加权面积。 然后，该方法计算并整合蛋白质的非药物值，找出曲线下的非药物区域。 最后，该方法通过比较曲线下的加权面积和蛋白质曲线下的非药物面积来评估一种或多种给药方案，以确定药物在该时间段内的净效应。

    Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
    9.
    发明授权
    Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA 失效
    酶鉴别增强方法和表面结合双链DNA

    公开(公告)号：US06974666B1

    公开(公告)日：2005-12-13

    申请号：US08533582

    申请日：1995-10-18

    申请人： David J. Lockhart ,  Mark S. Chee ,  Dirk Vetter ,  Martin Digglemann

    发明人： David J. Lockhart ,  Mark S. Chee ,  Dirk Vetter ,  Martin Digglemann

    IPC分类号： B01J19/00 ,  C07B61/00 ,  C07H21/00 ,  C12N15/10 ,  C12Q1/68 ,  C40B40/06 ,  G01N33/53 ,  G01N33/551 ,  C07H21/02 ,  C12P19/34

    CPC分类号： C12N15/1093 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00659 ,  B01J2219/00722 ,  C07B2200/11 ,  C07H21/00 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6837 ,  C12Q1/6874 ,  C40B40/06 ,  C40B80/00 ,  G01N33/5308 ,  G01N33/551

    摘要： Methods for discriminating between fully complementary hybrids and those that differ by one or more base pairs and libraries of unimolecular, double-stranded oligonucleotides on a solid support. In one embodiment, the present invention provides methods of using nuclease treatment to improve the quality of hybridization signals on high density oligonucleotide arrays. In another embodiment, the present invention provides methods of using ligation reactions to improve the quality of hybridization signals on high density oligonucleotide arrays. In yet another embodiment, the present invention provides libraries of unimolecular or intermolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a related aspect, the present invention provides libraries of conformationally restricted probes on a solid support. The probes are restricted in their movement and flexibility using double-stranded oligonucleotides as scaffolding. The probes are also useful in various screening procedures associated with drug discovery and diagnosis. The present invention further provides methods for the preparation and screening of the above libraries.

    摘要翻译： 用于区分完全互补的杂交体与通过一个或多个碱基对不同的那些的方法和在固体支持物上的单分子双链寡核苷酸的文库。 在一个实施方案中，本发明提供了使用核酸酶处理来提高高密度寡核苷酸阵列上杂交信号质量的方法。 在另一个实施方案中，本发明提供了使用连接反应来提高高密度寡核苷酸阵列上杂交信号质量的方法。 在另一个实施方案中，本发明提供了在固体支持物上的单分子或分子间双链寡核苷酸的文库。 这些文库在药物发现中可用于筛选许多生物样品，用于双链寡核苷酸与肽，蛋白质，药物和RNA之间的特异性相互作用。 在相关方面，本发明提供了在固体支持物上的构象限制探针的文库。 使用双链寡核苷酸作为脚手架，探针的运动和灵活性受到限制。 探针也可用于与药物发现和诊断相关的各种筛选程序。 本发明还提供了制备和筛选上述文库的方法。