公开(公告)号：US09133506B2

公开(公告)日：2015-09-15

申请号：US12087543

申请日：2007-01-09

Applicant: Nir Katzir ,  Tsafrir Kolatt ,  Irit Bar-Am

Inventor： Nir Katzir ,  Tsafrir Kolatt ,  Irit Bar-Am

IPC: G06F19/20 ,  C12Q1/06 ,  C12Q1/68 ,  G06K9/00

CPC classification number: C12Q1/6841 ,  G06K9/00134

Abstract: Methods, computer readable storage media and systems which can be used for analyzing labeled biological samples, identifying chromosomal aberrations, identifying genetically abnormal cells and/or computationally scanning the samples using randomly or randomized scanning methods are provided. Specifically, the present invention can be used to analyze FISH-stained samples and automatically identify chromosomal aberrations associated with abnormal intensity ratio of stained occurrences in the sample.

Abstract translation: 提供了可用于分析标记的生物样品，识别染色体畸变，识别遗传异常细胞和/或使用随机或随机扫描方法计算扫描样品的方法，计算机可读存储介质和系统。 具体地，本发明可用于分析FISH染色的样品，并自动识别与样品中染色事件的异常强度比相关的染色体畸变。

公开(公告)号：US20020099295A1

公开(公告)日：2002-07-25

申请号：US09776871

申请日：2001-02-06

Applicant: Applied Spectral Imaging Ltd.

Inventor： Amir Gil ,  Tamir Gil ,  Eli Horn ,  Yuval Garini

IPC: A61B005/00

CPC classification number: A61B5/14553 ,  A61B5/0042 ,  A61B5/0066 ,  A61B5/0073 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/0086 ,  A61B5/4064 ,  A61B5/4094

Abstract: A method of functional brain mapping of a subject is disclosed. The method is effected by (a) illuminating an exposed cortex of a brain or portion thereof of the subject with incident light; (b) acquiring a reflectance spectrum of each picture element of at least a portion of the exposed cortex of the subject; (c) stimulating the brain of the subject; (d) during or after step (c) acquiring at least one additional reflectance spectrum of each picture element of at least the portion of the exposed cortex of the subject; and (e) generating an image highlighting differences among spectra of the exposed cortex acquired in steps (b) and (d), so as to highlight functional brain regions. Algorithms for calculating oxygen saturation and blood volume maps which can be used to practice the method are also disclosed. Systems for practicing the method are also disclosed.

Abstract translation: 公开了一种对象的功能性脑图谱的方法。 该方法通过以下方式实现：（a）用入射光照射受试者的大脑或其部分的暴露皮层; （b）获取被摄体的暴露皮质的至少一部分的每个图像元素的反射光谱; （c）刺激受试者的大脑; （d）在步骤（c）期间或之后（c）获取受试者暴露皮层的至少部分的每个图像元素的至少一个附加反射光谱; 和（e）产生突出显示在步骤（b）和（d）中获得的暴露皮质的光谱之间的差异的图像，以便突出功能性脑区域。 还公开了可用于实践该方法的用于计算氧饱和度和血容量图的算法。 还公开了用于实施该方法的系统。

公开(公告)号：US6088099A

公开(公告)日：2000-07-11

申请号：US91756

申请日：1998-06-19

Applicant: Dario Cabib ,  Robert A. Buckwald

Inventor： Dario Cabib ,  Robert A. Buckwald

IPC: G01B9/02

CPC classification number: G01B9/02

Abstract: A method for spatial registration and spectral correction for interferometer based spectral imaging which can be used to obtain spectral images of a moving object, the method comprising the steps of (a) using an interferometer based spectral imager for acquiring spatial and spectral information of the moving object; and (b) correcting the spatial and spectral information for movements of the moving object via a spatial registration and spectral correction procedures for obtaining corrected spatial and spectral information.

Abstract translation: PCT No.PCT / US97 / 08153 Sec。 371日期：1998年6月19日 102（e）1998年6月19日PCT PCT 1997年5月12日PCT公布。 WO98 / 19293 PCT出版物 日期1998年5月7日一种用于基于干涉仪的光谱成像的空间配准和光谱校正方法，其可用于获得移动物体的光谱图像，该方法包括以下步骤：（a）使用基于干涉仪的光谱成像器获取空间和光谱 移动物体的信息; 以及（b）经由用于获得校正的空间和频谱信息的空间配准和频谱校正程序来校正用于移动物体的移动的空间和频谱信息。

公开(公告)号：US6007996A

公开(公告)日：1999-12-28

申请号：US122704

申请日：1998-07-27

Applicant: George McNamara ,  Dirk Soenksen ,  Dario Cabib ,  Robert A. Buckwald

Inventor： George McNamara ,  Dirk Soenksen ,  Dario Cabib ,  Robert A. Buckwald

IPC: G01B11/00 ,  A61B3/00 ,  A61B3/12 ,  A61B5/00 ,  A61B10/00 ,  C12Q1/68 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/443 ,  G01J3/45 ,  G01J3/453 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G02B21/36 ,  G06K9/00 ,  G06K9/76 ,  G01N33/53

CPC classification number: G01N21/6458 ,  C12Q1/6841 ,  C12Q1/6883 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G06K9/00127 ,  G06K9/76 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6417 ,  G01N2021/6423

Abstract: A method of in situ analysis of a biological sample comprising the steps of (a) staining the biological sample with N stains of which a first stain is selected from the group consisting of a first immunohistochemical stain, a first histological stain and a first DNA ploidy stain, and a second stain is selected from the group consisting of a second immunohistochemical stain, a second histological stain and a second DNA ploidy stain, with provisions that N is an integer greater than three and further that (i) if the first stain is the first immunohistochemical stain then the second stain is either the second histological stain or the second DNA ploidy stain; (ii) if the first stain is the first histological stain then the second stain is either the second immunohistochemical stain or the second DNA ploidy stain; whereas (iii) if the first stain is the first DNA ploidy stain then the second stain is either the second immunohistochemical stain or the second histological stain; and (b) using a spectral data collection device for collecting spectral data from the biological sample, the spectral data collection device and the N stains are selected such that a spectral component associated with each of the N stains is collectable.

Abstract translation: 一种生物样品的原位分析方法，包括以下步骤：（a）染色具有N染色的生物样品，其中第一染色选自第一免疫组织化学染色，第一组织染色和第一DNA倍体 染色，第二染色选自第二免疫组织化学染色，第二组织学染色和第二DNA倍性染色，其规定N为大于3的整数，并且（i）如果第一染色是 第一次免疫组织化学染色，第二次染色是第二次组织染色或第二次DNA倍体染色; （ii）如果第一染色是第一组织染色，则第二染色是第二免疫组织化学染色或第二DNA倍体染色; 而（iii）如果第一个染色是第一个DNA倍性染色，则第二次染色是第二次免疫组化染色或第二次组织染色; 和（b）使用用于收集来自生物样品的光谱数据的光谱数据采集装置，选择光谱数据采集装置和N染色，使得与每个N染色体相关联的光谱分量是可收集的。

公开(公告)号：US5936731A

公开(公告)日：1999-08-10

申请号：US575191

申请日：1995-12-20

Applicant: Dario Cabib ,  Robert A. Buckwald ,  David Wine ,  Yuval Garini ,  Nir Katzir ,  Dirk G. Soeknsen ,  Moshe Lavi

Inventor： Dario Cabib ,  Robert A. Buckwald ,  David Wine ,  Yuval Garini ,  Nir Katzir ,  Dirk G. Soeknsen ,  Moshe Lavi

IPC: G01B9/02 ,  A61B3/12 ,  A61B5/00 ,  C12Q1/68 ,  G01B11/06 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G06K9/00 ,  G06K9/76

CPC classification number: G01N21/6458 ,  A61B3/12 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6883 ,  G01B11/0675 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J3/4535 ,  G01N21/6428 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G06K9/00127 ,  G06K9/76 ,  A61B3/1241 ,  C12Q2600/156 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6417 ,  G01N2021/6441

Abstract: A fluorescent in situ hybridization method comprising the steps of (a) providing a cell nuclei having chromosomes hybridized with at least one nucleic acid probe including at least one nucleic acid molecule labeled with at least one fluorophore; (b) viewing the cell nuclei through a fluorescence microscope optically connected to an imaging spectrometer for obtaining a spectrum of each pixel of the cell nuclei by (i) collecting incident collimated light simultaneously from all pixels of the cell nuclei; (ii) passing the incident collimated light through an interferometer system so that the light is first split into two coherent beams and then recombine to interfere and form an exiting light beam; (iii) focusing the exiting light beam on a detector having an array of detector elements, so that at each instant each of the elements is the image of one and always the same pixel for the entire duration of the measurement and so that each of the elements produces a signal which is a particular linear combination of light intensity emitted by the pixel at different wavelengths; (iv) rotating or translating one or more of the elements of the interferometer, so that the optical path difference is scanned simultaneously for all the pixels of the cell nuclei; and (v) recording signals of each of the detector elements as function of time using a recording device to form a first spectral cube of data; and (c) interpreting the first spectral cube of data using a mathematical algorithm.

Abstract translation: 荧光原位杂交方法包括以下步骤：（a）提供具有染色体的细胞核，所述染色体与至少一种核酸探针杂交，所述核酸探针包括至少一个用至少一个荧光团标记的核酸分子; （b）通过光学连接到成像光谱仪的荧光显微镜观察细胞核，通过（i）从细胞核的所有像素同时收集入射准直光，以获得细胞核的每个像素的光谱; （ii）使入射的准直光通过干涉仪系统，使得光首先分裂成两个相干光束，然后重组以干涉并形成出射的光束; （iii）将出射的光束聚焦在具有检测器元件阵列的检测器上，使得在每个时刻，在整个测量持续时间内，每个元件是一个并且总是相同像素的图像，并且使得每个 元件产生信号，其是由不同波长的像素发射的光强度的特定线性组合; （iv）旋转或平移干涉仪的一个或多个元件，使得对于细胞核的所有像素同时扫描光程差; 以及（v）使用记录装置记录每个检测器元件的信号作为时间的函数，以形成数据的第一光谱立方体; 和（c）使用数学算法解释数据的第一个频谱立方体。

公开(公告)号：US20090304244A1

公开(公告)日：2009-12-10

申请号：US12385532

申请日：2009-04-10

Applicant: Tsafrir Kolatt ,  Eldad Klaiman

Inventor： Tsafrir Kolatt ,  Eldad Klaiman

IPC: G06K9/00 ,  G01N1/30

CPC classification number: G01N1/30

Abstract: A method for presenting at least one structure of a histological specimen. The method comprises providing a plurality of images of a plurality of sections of a single histological specimen having a plurality of structures, segmenting a plurality of segments of the plurality of structures in each the image, associating among respective the segments of a common structure of the plurality of structures, and presenting the association in relation to at least some of the plurality of images.

Abstract translation: 一种用于呈现组织学样本的至少一个结构的方法。 该方法包括提供具有多个结构的单个组织学样本的多个部分的多个图像，分割每个图像中的多个结构的多个部分，将相应的各个部分的共同结构相关联 多个结构，并且呈现与所述多个图像中的至少一些相关联的关联。

公开(公告)号：US20020141624A1

公开(公告)日：2002-10-03

申请号：US10155647

申请日：2002-05-28

Applicant: Applied Spectral Imaging Ltd.

Inventor： Amir Gil ,  Tamir Gil ,  Eli Horn ,  Yuval Garini

IPC: G06K009/00

CPC classification number: A61B5/0084 ,  A61B5/0075 ,  A61B5/0456 ,  A61B5/14553 ,  A61B5/4064 ,  A61B5/7285 ,  A61B6/541

Abstract: A synchronizing imaging apparatus to obtain images from an object undergoing variations according to a cycle with the apparatus comprising an acquisition device to acquire a plurality of pre-images at respective phases over each one of a plurality of cycles, and an image matcher to match together the pre-images from different ones of said cycles according to respective phases within said cycles, to create a representation of said cycle.

Abstract translation: 一种同步成像装置，用于根据周期从经历变化的对象获得图像，该装置包括：采集装置，用于在多个周期中的每一个周期获取各个相位的多个预图像;以及图像匹配器，以一起匹配 根据所述循环内的各个相位，来自不同的所述循环的预图像，以创建所述周期的表示。

公开(公告)号：US5991028A

公开(公告)日：1999-11-23

申请号：US824234

申请日：1997-03-25

Applicant: Dario Cabib ,  Robert A. Buckwald ,  Zvi Malik ,  Nissim Ben-Yosef

Inventor： Dario Cabib ,  Robert A. Buckwald ,  Zvi Malik ,  Nissim Ben-Yosef

IPC: G01B9/02 ,  C12N5/06 ,  C12Q1/68 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01J5/10 ,  G01N15/14 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G06K9/00 ,  G06K9/76

CPC classification number: C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6883 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J5/10 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G06K9/00127 ,  G06K9/76 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6417

Abstract: According to the present invention there is provided a spectral bio-imaging methods which can be used for automatic and/or semiautomatic spectrally resolved morphometric classification of cells, the method comprising the steps of (a) preparing a sample to be spectrally imaged, the sample including at least a portion of at least one cell; (b) viewing the sample through an optical device, the optical device being optically connected to an imaging spectrometer, the optical device and the imaging spectrometer being for obtaining a spectrum of each pixel of the sample; (c) classifying each of the pixels into classification groups according to the pixels spectra; and (d) analyzing the classification groups and thereby classifying the at least one cell into a cell class.

Abstract translation: 根据本发明，提供了可用于细胞的自动和/或半自动光谱解析形态学分类的光谱生物成像方法，该方法包括以下步骤：（a）制备待光谱成像的样品，样品 包括至少一个细胞的至少一部分; （b）通过光学装置观察样品，光学装置光学连接到成像光谱仪，光学装置和成像光谱仪用于获得样品的每个像素的光谱; （c）根据像素光谱将每个像素分类成分类组; 和（d）分析分类组，从而将至少一个细胞分类成细胞类别。

公开(公告)号：US5798262A

公开(公告)日：1998-08-25

申请号：US844516

申请日：1997-04-18

Applicant: Yuval Garini ,  Nir Katzir ,  David Wine ,  Dario Cabib

Inventor： Yuval Garini ,  Nir Katzir ,  David Wine ,  Dario Cabib

IPC: G01N33/48 ,  C12M1/00 ,  C12M3/00 ,  C12N15/09 ,  C12N15/11 ,  C12Q1/68 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01N1/28 ,  G01N21/64 ,  G01N21/75 ,  G01N33/483 ,  G01N33/50 ,  G01N33/52 ,  G01N33/569 ,  G01N33/58 ,  G01N37/00 ,  G02B21/00 ,  G02B21/16 ,  G06K9/00 ,  G06K9/76 ,  C12M1/34 ,  G06F15/00

CPC classification number: G01J3/12 ,  C12Q1/6816 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G06K9/00127 ,  G06K9/0014 ,  G06K9/76 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6423 ,  G01N2021/6441

Abstract: A method for finding L internal reference vectors for classification of L chromosomes or portions of chromosomes of a cell, the L chromosomes or portions of chromosomes being painted with K different fluorophores or combinations thereof, wherein K basic chromosomes or portions of chromosomes of the L chromosomes or portions of chromosomes are each painted with only one of the K different fluorophores, whereas the other L-K of the L chromosomes or portions of chromosomes are each painted with a different combination of the K different fluorophores, the method comprising the steps of (a) using a multi-band collection device for measuring a first vector for each pixel of each of the L chromosomes or portions of chromosomes; (b) identifying pixels belonging to each of the K basic chromosomes or portions of chromosomes and defining the pixels as basic pixels, so as to obtain K basic classes of basic pixels; (c) using at least one basic pixel from each of the K basic classes for obtaining K basic vectors, the K basic vectors being K internal reference vectors; (d) using the K basic vectors for identifying pixels belonging to the other L-K chromosomes or portions of chromosomes; and (e) using the pixels belonging to the other L-K chromosomes or portions of chromosomes for calculating the other L-K internal reference vectors, thereby finding all of the L internal reference vectors. A method for classification of L chromosomes or portions of chromosomes of a cell similarly painted using the above method for finding L internal reference vectors, and using the L reference vectors for classification of each of the pixels into one of L classification classes. And, images presenting color chromosomes.

公开(公告)号：US11012643B2

公开(公告)日：2021-05-18

申请号：US15379519

申请日：2016-12-15

Applicant: Applied Spectral Imaging Ltd.

Inventor： Nir Katzir

IPC: H04N5/33 ,  G01J3/453 ,  G01J3/51 ,  G01J3/26 ,  G01B9/02 ,  G02B26/00 ,  H04N9/04 ,  G01J3/02 ,  G01J3/28 ,  G01J3/45 ,  G06T5/00 ,  H01L27/146

Abstract: A spectral imaging system comprises: a sequential optical system providing a temporal sequence of output light beams describing the scene; a color imager receiving the output light beams and responsively generating, for each output light beam, an image signal that is spatially resolved into a plurality of color channels. The system can also comprise an image processor that collectively process the image signals to construct a spectral image of the scene.