公开(公告)号：US12066413B2

公开(公告)日：2024-08-20

申请号：US17294710

申请日：2019-12-04

申请人： Cytiva Sweden AB

发明人： Daniel Salomonsson ,  Mats Lundkvist

IPC分类号： G01N30/60 ,  B01D15/20 ,  B01D15/22

CPC分类号： G01N30/6082 ,  B01D15/206 ,  B01D15/22

摘要： Disclosed is a chromatographic separation column assembly (100) comprising a column cylinder (120) having a cylinder wall (126) including an inner wall surface (124) partially defining a column volume (50), and a column component (110/130) insertable into to the cylinder (120), wherein at least an edge region (113/133) of the column component is intended to be in contact or adjacent a part of the inner wall surface (124) in use, the edge region and the contacting or adjacent part of inner wall surface each being formed from a compatible heat fusible material or materials, and wherein at least the cylinder wall in the area of the contacting or adjacent part of the inner wall is formed from a material which allows transmission of the light energy needed to cause said fusing of the adapter plate edge region to the inner wall surface.

公开(公告)号：US20240085382A1

公开(公告)日：2024-03-14

申请号：US17767795

申请日：2020-10-15

申请人： Cytiva Sweden AB

发明人： Stephan Menzel

IPC分类号： G01N30/02

CPC分类号： G01N30/02 ,  G01N2015/084

摘要： Disclosed is a system and a method for testing at least one filtration device. The system comprises a chromatography system (3) comprising at least one pair of inlet and outlet chromatography column connections (7a), 7b for at least one chromatography column, and at least one filtration device housing (21) comprising a filtration device (23) to be tested, whereby each filtration device housing (21) is connected to one of the at least one pair of inlet and outlet chromatography column connections (7a, 7b) instead of a chromatography column in the chromatography system.

公开(公告)号：US11927503B2

公开(公告)日：2024-03-12

申请号：US17276981

申请日：2019-09-09

申请人： Cytiva Sweden AB

发明人： Sameera Simha Hindupur ,  Manoj Kumar Ramakrishna ,  Andreas Castan ,  Swapnil Puranik ,  Klaus Gebauer

IPC分类号： G01M3/28 ,  B29C65/00 ,  B29C65/82 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34

CPC分类号： G01M3/2884 ,  B29C65/8246 ,  B29C66/522 ,  C12M23/40 ,  C12M37/04 ,  C12M41/40

摘要： The present invention relates to a computer implemented method for determining a weld integrity test result performed by a system configured to aseptically transferring cells to a container (BR), the method comprising welding (710) a vial to a connector to obtain a fluid-tight seal between the vial (V) and a connector (C), the connector being provided with a pair of conduits each having one end protruding through the connector and into the vial, sealing (720) an opposite end of one of the conduits, generating (730) a fluid pressure different to an ambient fluid pressure at an opposite end of the other conduit, measuring (740) a fluid pressure within at least one of the conduits, determining (750) a fluid pressure change based on the ambient pressure and the measured fluid pressure, determining (760) the weld integrity test result as pass or as fail based on the fluid pressure change.

公开(公告)号：US11886156B2

公开(公告)日：2024-01-30

申请号：US17130711

申请日：2020-12-22

申请人： Cytiva Sweden AB

发明人： Dalesh Dharamshi ,  Sudheer Mygapula

IPC分类号： G05B17/02 ,  G05B13/04

CPC分类号： G05B17/02 ,  G05B13/041 ,  G05B2219/23258 ,  G05B2219/23452 ,  G05B2219/32341 ,  G05B2219/37453 ,  Y02P90/02

摘要： A system for providing an improved way of user intuitive visual management of bioprocess automation is disclosed. The system includes a processor and a memory. The processor is configured to present a graphical presentation of the bioprocess automation script and results associated with execution of the bioprocess automation script of a bioprocess, wherein the graphical presentation comprises an anticipated result and an anticipated flow path associated with the bioprocess; and modify the graphical presentation by varying at least one of the anticipated result and the anticipated flow path.

公开(公告)号：US20240009594A1

公开(公告)日：2024-01-11

申请号：US18474000

申请日：2023-09-25

申请人： Cytiva Sweden AB

发明人： Bjorn Markus Olovsson

IPC分类号： B01D15/16 ,  G01N30/32 ,  G01N30/34 ,  B01F23/40 ,  B01F25/31 ,  B01F35/21 ,  B01F35/221 ,  B01F35/71 ,  B01D15/22

CPC分类号： B01D15/166 ,  G01N30/32 ,  G01N30/34 ,  B01F23/49 ,  B01F25/31 ,  B01F35/2132 ,  B01F35/2133 ,  B01F35/2211 ,  B01F35/7176 ,  B01D15/22 ,  G01N2030/347 ,  B01D15/16 ,  G01N30/8658

摘要： A system for preparing solutions for chromatography application is disclosed. The system comprises a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution. The T-joint receives the second solution from a solution supply unit connected to the T-joint. Further one or more low pressure pumps supply the one or more first solutions into the T-joint. The high pressure pump collects the buffer solution and delivers it to a chromatography apparatus.

公开(公告)号：US20230384741A1

公开(公告)日：2023-11-30

申请号：US18327662

申请日：2023-06-01

申请人： Cytiva Sweden AB

发明人： Mikael Johansson ,  Pasi Juntikka ,  Peter Toreheim ,  Shivakumar Selvaraj

IPC分类号： G05B13/02

CPC分类号： G05B13/0205

摘要： The present invention relates to a computer implemented method (600) for optimization of a bioprocessing system (100) formed by interconnected or interconnectable bioprocessing units and configured to provide a desired system functionality, the method comprising obtaining (610) requirement data of a bioprocess, the requirement data being at least indicative of the desired system functionality, and indicative of a desired substance resulting from the operation of the bioprocess system, obtaining (620) unit data indicative of characteristics of a plurality of bioprocessing units, generating (630) at least one optimized or suitable bioprocessing system configuration capable to provide the desired system functionality, the bioprocessing system configuration comprising a selection of bioprocessing units from the plurality of bioprocessing units selected using the requirement data, the unit data and an objective function dependent on the requirement data and the unit data. The invention extends to a configuration system (500).

公开(公告)号：US11717790B2

公开(公告)日：2023-08-08

申请号：US17252358

申请日：2019-06-20

申请人： Cytiva Sweden AB

发明人： Nityananda Maiya ,  Deepak Aravind ,  Fredrik Lundström ,  Saameera Simha Hindupur ,  Shivakumar Selvaraj ,  Alagur Sahebagouda

IPC分类号： B01D61/12 ,  B01D61/22 ,  B01D65/02

CPC分类号： B01D61/12 ,  B01D61/22 ,  B01D65/02 ,  B01D2311/04 ,  B01D2311/06 ,  B01D2311/14 ,  B01D2311/16 ,  B01D2313/48 ,  B01D2315/10 ,  B01D2315/20 ,  B01D2321/40

摘要： A method includes measuring, by a flow sensor (70), a flow rate of a permeate fluid (48) flowing through a filter (20) of a membrane filtration system (12). Further, the method includes receiving, by a control unit (86), the measured flow rate of the permeate fluid (48) and determining, by the control unit (86), a first flux rate of the filter (20) based on the measured flow rate of the permeate fluid (48). Furthermore, the method includes comparing, by the control unit (86), the determined first flux rate with a first predetermined flux rate. Additionally, the method includes operating the membrane filtration system (12), by the control unit (86), in a normal mode or a flux tolerant mode based on the comparison of the determined first flux rate with the first predetermined flux rate. The flux tolerant mode of the membrane filtration system (12) is further based on a determined normalized water permeability value of the filter (20).

公开(公告)号：US20230204495A1

公开(公告)日：2023-06-29

申请号：US17996581

申请日：2021-04-27

申请人： CYTIVA SWEDEN AB

发明人： Hanno Ehring ,  David Gronowitz ,  Lars Rosengren

IPC分类号： G01N21/27 ,  G01N21/31

CPC分类号： G01N21/274 ,  G01N21/31

摘要： The present invention relates to computer implemented method performed by a computer (110) configured to calibrate an apparatus for measuring the absorbance of light of a substance (131) for a chromatography system (100), the apparatus (131) comprising a conduit (C) for enabling a fluid to be measured, a light emitter (LE) configured to emit light along an optical path (OP) to a light sensor (S) configured to measure the emitted light, the optical path intersecting the conduit (C), a rotating disc (D) having one or more optical filters, each of the one or more optical filters is arranged with its center passing through the optical path (OP) when rotating, an actuator configured to rotate the disc (D) dependent on a control signal, to the method comprising controlling rotation of (710) the disc (D) to a first position where a first filter, of the one or more optical filters, intersects the optical path (OP) at a first point (P1), measuring (720) a first light absorption value, controlling rotation of (730) the disc (D) to a second position, different to the first position, where the first filter still intersects the optical path (OP) at a second point (P2), measuring (740) a second light absorption value, generating (750) an aggregated light absorption value, calibrating (760) the apparatus (131) by comparing the aggregated light absorption value to a reference light absorption value.

公开(公告)号：US11670402B2

公开(公告)日：2023-06-06

申请号：US16130092

申请日：2018-09-13

申请人： GE Healthcare Bio-Sciences AB

发明人： Enrique Carredano

IPC分类号： G16C20/30 ,  G16C99/00 ,  G01N27/06 ,  B01D15/12 ,  G01N27/416 ,  G01N30/34

CPC分类号： G16C20/30 ,  B01D15/12 ,  G01N27/06 ,  G01N27/4166 ,  G16C99/00 ,  G01N30/34

摘要： In a method of preparing a liquid solution by mixing ingredients according to a predetermined recipe, wherein at least one pair of species of the liquid solution is derived from a weak electrolyte and corresponds to an acid-base pair, the conductivity of the liquid solution is predicted by:



(i) for each pair of species derived from a weak electrolyte, solving a respective equilibrium equation to calculate the actual molar concentration of each such species at equilibrium in the liquid solution,
(ii) calculating for each ionic species of said plurality of species the molar conductivity by the formula:

Λ=Λ0−K×Sqrt(c)

wherein Λ is the molar conductivity, Λ0 is the molar conductivity at infinite dilution, c is the concentration, and K is the Kohlrausch coefficient, and wherein K and Λ0 are predetermined values for K and Λ0 for each ionic species,
(iii) calculating the conductivity κ for each ionic species by the formula:

κ=c×Λ

and
(iv) adding up the conductivities determined in step (iii) for the different ionic species to obtain a predicted conductivity of the liquid solution. A computer program product comprises instructions for causing a computer to perform the method steps.

公开(公告)号：US20230159958A1

公开(公告)日：2023-05-25

申请号：US17995571

申请日：2021-04-06

申请人： CYTIVA SWEDEN AB

发明人： Andreas Jonsson ,  Daniel Ivansson

IPC分类号： C12N15/90 ,  C12N9/12 ,  C12N15/85

CPC分类号： C12N15/907 ,  C12N9/12 ,  C12N15/85 ,  C12N2800/30 ,  C12N2800/106

摘要： The present disclosure relates to a method for targeted integration of a donor vector into a specific pre-defined genomic location of an isolated eukaryotic host cell. The vector and host cell together comprise nucleic acid components allowing for the selection of cells having integrated the donor vector into the pre-defined genomic location of the host cell. In addition, it provides for the identification of any random integrations of the donor vector(s) into other parts of the host cell genome. Once identified, such cells present an excellent alternative for subsequent recombinant protein production.