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154 条记录 (耗时 0.054 秒)

    Tissue processing apparatus
    1.
    发明授权
    Tissue processing apparatus 有权

    公开(公告)号：US10416051B2

    公开(公告)日：2019-09-17

    申请号：US16007756

    申请日：2018-06-13

    申请人： Dako Denmark A/S

    发明人： Tim Svenstrup Poulsen ,  Steen Hauge Matthiesen

    IPC分类号： G01N1/00 ,  G01N1/28 ,  G01N1/31 ,  G01N1/30

    摘要： An apparatus (100) for processing a biological sample (101). The biological sample being arranged on a first planar surface (102) of a carrier (103). The apparatus comprises a second planar surface (104) arranged substantially parallel to said first planar surface and at a first distance from said first planar surface, said first planar surface and said second planar surface are arranged at an angle (A) greater than zero degree from the horizontal plane (HP); supply means (126, 131, 220) for supplying an amount (105) of a liquid that is to be applied to said biological sample. The first planar surface and second planar surface are configured to be arranged at a second distance from each other, said second distance being such that said supplied amount of liquid is distributed over said biological sample when said first planar surface and said second planar surface are brought to said second distance from each other.

    Conjugate molecules
    5.
    发明授权
    Conjugate molecules 有权

    公开(公告)号：US09671400B2

    公开(公告)日：2017-06-06

    申请号：US12708702

    申请日：2010-02-19

    申请人： Jesper Lohse

    发明人： Jesper Lohse

    IPC分类号： C12Q1/28 ,  G01N33/543 ,  C08G63/672 ,  C08G69/40 ,  C08G69/48

    CPC分类号： G01N33/54393 ,  C08G63/672 ,  C08G69/40 ,  C08G69/48 ,  C12Q1/28

    摘要： The present invention relates to compounds which are conjugates of two or more moieties capable of serving as substrates of an enzyme with peroxidase activity and one or more labels. The conjugate molecules of the invention are useful for detection of molecular targets, e.g. biological or chemical molecules, molecular structures, etc, in samples using a host of experimental schemes for detecting and visualizing such targets, e.g. immunohistochemistry (IHC), in situ hybridization (ISH), ELISA, Southern, Northern, and Western blotting, etc. The invention also relates to compositions comprising the conjugate molecules.

    IN SITU HYBRIDIZATION DETECTION METHOD
    7.
    发明申请
    IN SITU HYBRIDIZATION DETECTION METHOD 审中-公开
    原位杂交检测方法

    公开(公告)号：US20160273035A1

    公开(公告)日：2016-09-22

    申请号：US14659847

    申请日：2015-03-17

    申请人： DAKO DENMARK A/S

    发明人： Lars Winther ,  Jesper Lohse ,  Susanne Gabs ,  Kenneth Heesche Petersen

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6876 ,  C12Q1/6841 ,  C12Q2525/161 ,  C12Q2565/40

    摘要： The invention provides compositions and methods for the detection of targets in a sample; in particular, an in situ hybridization (ISH) sample. Probes and detectable labels may be provided in multiple layers in order to increase the flexibility of a detection system, and to allow for amplification to enhance the signal from a target. The layers may be created by incorporating probes and detectable labels into larger molecular units that interact through nucleic acids base-pairing, including peptide-nucleic acid (PNA) base-pairing. Optional non-natural bases allow for degenerate base pairing schemes. The compositions and methods are also compatible with immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry, enzyme immuno-assays (EIA), enzyme linked immuno-assays (ELISA), blotting methods (e.g. Western, Southern, and Northern), labeling inside electrophoresis systems or on surfaces or arrays, and precipitation, among other general detection assay formats. The invention is also compatible with many different types of targets, probes, and detectable labels.

    摘要翻译： 本发明提供用于检测样品中靶标的组合物和方法; 特别是原位杂交（ISH）样品。 可以以多层提供探针和可检测标记，以便增加检测系统的灵活性，并允许放大以增强来自靶的信号。 可以通过将探针和可检测标记掺入通过核酸碱基配对（包括肽 - 核酸（PNA）碱基配对）相互作用的较大分子单元中来产生层。 可选的非天然碱基允许简并碱基配对方案。 组合物和方法还与免疫组化（IHC），免疫细胞化学（ICC），流式细胞术，酶免疫测定（EIA），酶联免疫测定（ELISA），印迹方法（例如西方，南方和北方）相兼容， 标记电泳系统或表面或阵列，以及其他一般检测测定形式的沉淀。 本发明还与许多​​不同类型的靶，探针和可检测标记相兼容。

    Method for enzyme-mediated signal amplification
    8.
    发明授权
    Method for enzyme-mediated signal amplification 有权
    酶介导信号放大方法

    公开(公告)号：US09366675B2

    公开(公告)日：2016-06-14

    申请号：US14112808

    申请日：2012-04-19

    申请人： Jesper Lohse ,  Galina Skladtchikova

    发明人： Jesper Lohse ,  Galina Skladtchikova

    IPC分类号： G01N33/00 ,  C12Q1/26 ,  C12Q1/28 ,  G01N33/58 ,  G01N33/574

    CPC分类号： G01N33/581 ,  C12Q1/26 ,  C12Q1/28 ,  G01N33/57492

    摘要： The present invention relates to methods and compounds for enzyme-mediated amplification of target-associated signals for visualization of biological or chemical targets in samples, wherein the targets are immobilized, in particular the invention relates to the oxidoreductase-mediated signal amplification for visualization of targets in samples comprising cells. The methods of the invention are particular useful for qualitative and quantitative evaluation of biological markers relating to medical diagnostic and therapy.

    摘要翻译： 本发明涉及用于酶介导扩增靶标相关信号的方法和化合物，用于在样品中生物或化学靶标的可视化，其中靶标被固定，特别是本发明涉及用于目标可视化的氧化还原酶介导的信号放大 在包含细胞的样品中。 本发明的方法对于与医学诊断和治疗有关的生物学标记的定性和定量评估特别有用。