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公开(公告)号：US20170044090A1
公开(公告)日：2017-02-16
申请号：US15306363
申请日：2014-10-29
申请人： Solazyme, Inc.
IPC分类号： C07C69/675 , C08G18/36
CPC分类号： C08G18/36 , A23L33/115 , C07C67/31 , C10L1/026 , C11C3/00 , C11C3/003 , C11D1/66 , C11D3/188 , C11D3/2024 , C11D3/2079 , C11D17/0017 , Y02E50/13 , Y02T50/678 , C07C69/708
摘要： This invention relates to a ring opening method. The method includes reacting an epoxidized fatty acid ester with an alcohol to open an epoxy ring of the ester. A salt of tetrafluoroboric acid is used as a catalyst in the reaction. In another embodiment, the invention relates to a method of producing a polyurethane. An epoxidized fatty acid ester is reacted with an alcohol to open an epoxy ring of the ester, thereby to produce a polyol. A salt of tetrafluoroboric acid is used as a catalyst in the reaction. The polyol is then reacted with an isocyanate to produce a polyurethane. In a further embodiment, the polyol is used to produce a product selected from polyurethanes, coatings, adhesives, sealants, elastomers and lubricants.
摘要翻译： 本发明涉及一种开环方法。 该方法包括使环氧化脂肪酸酯与醇反应以打开酯的环氧环。 在反应中使用四氟硼酸的盐作为催化剂。 在另一个实施方案中，本发明涉及一种生产聚氨酯的方法。 将环氧化脂肪酸酯与醇反应以打开酯的环氧环，由此制备多元醇。 在反应中使用四氟硼酸的盐作为催化剂。 然后将多元醇与异氰酸酯反应以制备聚氨酯。 在另一个实施方案中，多元醇用于生产选自聚氨酯，涂料，粘合剂，密封剂，弹性体和润滑剂的产品。
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公开(公告)号：US09290749B2
公开(公告)日：2016-03-22
申请号：US13837996
申请日：2013-03-15
申请人： Solazyme, Inc.
CPC分类号： C12P7/6409 , A23L33/115 , A23V2002/00 , C10L1/02 , C10L2200/0476 , C10L2290/26 , C12N9/16 , C12P7/6463 , C12Y301/02014
摘要： The invention features plant acyl-ACP thioesterase genes of the FatB class and proteins encoded by these genes. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Oleaginous microalga host cells with the new genes or previously identified FatB genes are disclosed. The microalgae cells produce triglycerides with useful fatty acid profiles.
摘要翻译： 本发明具有FatB类的植物酰基-ACP硫酯酶基因和由这些基因编码的蛋白质。 该基因可用于构建具有改变的脂肪酸谱的重组宿主细胞。 公开了具有新基因或先前鉴定的FatB基因的嗜酸微藻宿主细胞。 微藻细胞产生具有有用脂肪酸分布的甘油三酯。
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3.发明授权公开(公告)号：US09279136B2
公开(公告)日：2016-03-08
申请号：US13630757
申请日：2012-09-28
申请人： Scott Franklin , Aravind Somanchi , Janice Wee , George Rudenko , Jeffrey L. Moseley , Walt Rakitsky
发明人： Scott Franklin , Aravind Somanchi , Janice Wee , George Rudenko , Jeffrey L. Moseley , Walt Rakitsky
IPC分类号： C12N9/16 , C12N1/13 , C12N15/67 , C12N15/79 , C12N15/82 , C12P7/64 , A23D7/00 , A23D9/00 , A23K1/16 , C11B1/00 , C11C3/00
CPC分类号： C12N15/79 , A23D7/00 , A23D9/00 , A23K20/158 , C11B1/00 , C11C3/00 , C12N9/0071 , C12N9/16 , C12N9/2431 , C12N9/2465 , C12N15/67 , C12N15/8216 , C12P7/64 , C12P7/6445 , C12P7/6463 , C12Y114/19001 , C12Y301/02 , C12Y301/02014 , C12Y302/01022 , C12Y302/01026 , Y02E50/13 , Y02P30/20 , Y02T50/678
摘要： Methods and compositions for the production of food compositions, oils, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing food compositions, and transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
摘要翻译： 提供了用于在重组微生物中生产食品组合物，油，燃料，油脂化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪醛脱羰酶和/或酰基载体蛋白质可用于制造食品组合物，以及运输燃料如可再生柴油，生物柴油和可再生喷气燃料， 以及诸如功能流体，表面活性剂，肥皂和润滑剂的油脂化学品。
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公开(公告)号：US09109239B2
公开(公告)日：2015-08-18
申请号：US13650018
申请日：2012-10-11
申请人： Scott Franklin , Aravind Somanchi , Janice Wee , George Rudenko , Jeffrey L. Moseley , Walt Rakitsky
发明人： Scott Franklin , Aravind Somanchi , Janice Wee , George Rudenko , Jeffrey L. Moseley , Walt Rakitsky
IPC分类号： A23L2/00 , A23D7/00 , A23D9/00 , A23C9/12 , C11C1/00 , C07C51/00 , C11C3/00 , A01N55/06 , A61K31/305 , A01N37/00 , C12P7/64 , A23K1/16 , C11B1/00
CPC分类号： C12N15/79 , A23D7/00 , A23D9/00 , A23K20/158 , C11B1/00 , C11C3/00 , C12N9/0071 , C12N9/16 , C12N9/2431 , C12N9/2465 , C12N15/67 , C12N15/8216 , C12P7/64 , C12P7/6445 , C12P7/6463 , C12Y114/19001 , C12Y301/02 , C12Y301/02014 , C12Y302/01022 , C12Y302/01026 , Y02E50/13 , Y02P30/20 , Y02T50/678
摘要： Methods and compositions for the production of food compositions, oils, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing food compositions, and transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
摘要翻译： 提供了用于在重组微生物中生产食品组合物，油，燃料，油脂化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪醛脱羰酶和/或酰基载体蛋白质可用于制造食品组合物，以及输送燃料如可再生柴油，生物柴油和可再生喷气燃料， 以及诸如功能流体，表面活性剂，肥皂和润滑剂的油脂化学品。
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公开(公告)号：US09062294B2
公开(公告)日：2015-06-23
申请号：US13555009
申请日：2012-07-20
IPC分类号： C12N9/20 , C12N9/24 , C12P7/64 , C10L1/02 , C12N9/00 , C12N9/16 , C12P7/20 , C12N15/79 , C07C1/207
CPC分类号： C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
摘要： Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention.
摘要翻译： 本文公开了通过制备新型甘油三酯油然后化学改性油来制造可再生化学品的方法。 公开了酯交换，氢化，加氢裂化，脱氧，异构化，酯交换，羟基化，水解和皂化等方法。 还公开了含有C8，C10，C12或C14脂肪酸链长度的新型油，并且可用作本发明方法中的原料。
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公开(公告)号：US08951777B2
公开(公告)日：2015-02-10
申请号：US13852116
申请日：2013-03-28
申请人： Solazyme, Inc.
IPC分类号： C12N1/12 , C12N15/82 , C12N9/24 , C12P7/64 , C10L1/02 , C12N9/00 , C12N9/16 , C12P7/20 , C12N15/79 , C07C1/207
CPC分类号： C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
摘要： Disclosed herein are obligate heterotrophic microalgae cells containing an exogenous gene. In some embodiments the gene is a sucrose utilization gene, and further disclosed are methods of manufacturing triglyceride oils using sugar cane or sugar beets as a feedstock in a heterotrophic fermentation. In other embodiments the feedstock is depolymerized cellulosic material. Also disclosed are cells that produce medium chain fatty acids at levels not produced in non-recombinant cells of the same species and genus.
摘要翻译： 本文公开了含有外源基因的专性异养微藻细胞。 在一些实施方案中，该基因是蔗糖利用基因，并且进一步公开了使用甘蔗或甜菜作为异养发酵中的原料来制备甘油三酯油的方法。 在其它实施方案中，原料是解聚的纤维素材料。 还公开了以相同物种和属的非重组细胞中不产生的水平产生中链脂肪酸的细胞。
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公开(公告)号：US08772575B2
公开(公告)日：2014-07-08
申请号：US13628039
申请日：2012-09-26
CPC分类号： C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
摘要： Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.
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公开(公告)号：US08697427B2
公开(公告)日：2014-04-15
申请号：US13479200
申请日：2012-05-23
CPC分类号： C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
摘要： Disclosed herein are obligate heterotrophic microalgae cells containing an exogenous gene. In some embodiments the gene is a sucrose utilization gene, and further disclosed are methods of manufacturing triglyceride oils using sugar cane or sugar beets as a feedstock in a heterotrophic fermentation. In other embodiments the feedstock is depolymerized cellulosic material. Also disclosed are cells that produce medium chain fatty acids at levels not produced in non-recombinant cells of the same species and genus.
摘要翻译： 本文公开了含有外源基因的专性异养微藻细胞。 在一些实施方案中，该基因是蔗糖利用基因，并且进一步公开了使用甘蔗或甜菜作为异养发酵中的原料来制备甘油三酯油的方法。 在其它实施方案中，原料是解聚的纤维素材料。 还公开了以相同物种和属的非重组细胞中不产生的水平产生中链脂肪酸的细胞。
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公开(公告)号：US08674180B2
公开(公告)日：2014-03-18
申请号：US13621722
申请日：2012-09-17
CPC分类号： C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
摘要： Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.
摘要翻译： 公开了来自微藻的新型基因序列，以及可用于制备甘油三酯油的新型基因序列。 还公开了允许将微藻种植在甘蔗和甜菜作为原料的序列和载体。 在一些实施方案中，载体可用于进行对异养微藻的核基因组的靶向修饰。
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公开(公告)号：US08633012B2
公开(公告)日：2014-01-21
申请号：US13650024
申请日：2012-10-11
申请人： Solazyme, Inc.
发明人： Scott Franklin , Aravind Somanchi , Janice Wee , George Rudenko , Jeffrey L. Moseley , Walt Rakitsky , Xinhua Zhao , Riyaz Bhat
CPC分类号： C12P7/6463 , C07C69/52 , C10L1/02 , C10L2200/0476 , C10L2290/26 , C10L2290/30 , C12N9/0071 , C12N9/1029 , C12N9/16 , C12N15/52 , Y02E50/13
摘要： Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
摘要翻译： 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪酸羟化酶，去饱和酶，脂肪醛脱羰酶和/或酰基载体蛋白，可用于制造运输燃料如可再生柴油，生物柴油， 和可再生喷气燃料，以及油化学品如功能流体，表面活性剂，肥皂和润滑剂。
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