公开(公告)号：US20160074511A1

公开(公告)日：2016-03-17

申请号：US14849379

申请日：2015-09-09

申请人： Korea University Research and Business Foundation

发明人： Jeewon Lee ,  Koo-Chul Kwon

IPC分类号： A61K41/00 ,  A61N5/06 ,  A61K49/08 ,  C07K14/005 ,  A61K49/14

CPC分类号： A61N5/062 ,  A61K41/0052 ,  A61K47/62 ,  A61K49/0428 ,  C07K14/005 ,  C07K14/47 ,  C07K14/71 ,  C07K14/78 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/32 ,  C07K2319/33 ,  C07K2319/735 ,  C12N2730/10122 ,  C12N2730/10123 ,  C12N2730/10133 ,  C12N2730/10142 ,  C12N2810/855

摘要： The present invention relates to a recombinant self-assembled protein comprising a target-oriented peptide and a use thereof. The recombinant self-assembled protein according to the present invention, comprising a target-oriented peptide, does not require an additional process for providing target-orientedness, and is thus capable of delivering a desired drug to a target tissue or target cell without using additives, such as chemical binders or stabilizers; therefore, the protein can be used for photothermal therapy, drug delivery, imaging, or the like. In particular, according to the present invention, it is to possible to prepare gold-protein nanoparticle fusions in which uniform high-density gold nanoparticles having target-orientedness are bound to protein surfaces, without an additional process of surface stabilization or process for providing target-orientedness. Compared with conventional gold nanoparticles, the gold-protein nanoparticle fusions according to the present invention show structural stability against pH variation and concentration variation, and also have excellent target-orientedness; therefore, the fusions can bring a dramatic enhancement to the utilization of gold nanoparticles in photothermal therapy.

摘要翻译： 本发明涉及包含靶向取向肽的重组自组装蛋白及其用途。 包含目标取向肽的根据本发明的重组自组装蛋白质不需要另外的提供靶向的方法，因此能够在不使用添加剂的情况下将期望的药物递送至靶组织或靶细胞 ，如化学粘合剂或稳定剂; 因此，蛋白质可以用于光热治疗，药物递送，成像等。 特别地，根据本发明，可以制备金蛋白纳米颗粒融合物，其中均匀的具有靶向取向性的高密度金纳米颗粒与蛋白质表面结合，而没有额外的表面稳定化过程或用于提供靶标的方法 方向性 与传统的金纳米颗粒相比，本发明的金蛋白纳米颗粒融合体显示出对pH变化和浓度变化的结构稳定性，并具有优异的靶向性; 因此，融合可以显着提高金纳米粒子在光热疗中的应用。

公开(公告)号：US09913886B2

公开(公告)日：2018-03-13

申请号：US15201029

申请日：2016-07-01

申请人： AnGes, Inc.

发明人： Mariko Kyutoku ,  Hironori Nakagami ,  Hiroshi Koriyama ,  Futoshi Nakagami ,  Ryuichi Morishita

IPC分类号： C12N7/00 ,  A61K39/29 ,  A61K39/00 ,  C12N15/85

CPC分类号： A61K39/0012 ,  A61K39/292 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/575 ,  A61K2039/58 ,  C12N7/00 ,  C12N15/85 ,  C12N2730/10142 ,  C12N2730/10143 ,  C12N2730/10171

摘要： The present invention provides an agent for the treatment or prophylaxis of arteriosclerosis comprising an expression vector encoding a chimeric Hepatitis B virus core antigen polypeptide inserted with an amino acid sequence containing a specific epitope of apolipoprotein (a), wherein the amino acid sequence containing the specific epitope is inserted between the amino acid residues 80 and 81 of the hepatitis B virus core antigen polypeptide.

公开(公告)号：US09814907B2

公开(公告)日：2017-11-14

申请号：US14849379

申请日：2015-09-09

申请人： Korea University Research and Business Foundation

发明人： Jeewon Lee ,  Koo-Chul Kwon

IPC分类号： A61K47/69 ,  A61N5/06 ,  C07K14/005 ,  A61K49/04 ,  C07K14/71

CPC分类号： A61N5/062 ,  A61K41/0052 ,  A61K47/62 ,  A61K49/0428 ,  C07K14/005 ,  C07K14/47 ,  C07K14/71 ,  C07K14/78 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/32 ,  C07K2319/33 ,  C07K2319/735 ,  C12N2730/10122 ,  C12N2730/10123 ,  C12N2730/10133 ,  C12N2730/10142 ,  C12N2810/855

摘要： The present invention relates to a recombinant self-assembled protein comprising a target-oriented peptide and a use thereof. The recombinant self-assembled protein according to the present invention, comprising a target-oriented peptide, does not require an additional process for providing target-orientedness, and is thus capable of delivering a desired drug to a target tissue or target cell without using additives, such as chemical binders or stabilizers; therefore, the protein can be used for photothermal therapy, drug delivery, imaging, or the like. In particular, according to the present invention, it is possible to prepare gold-protein nanoparticle fusions in which uniform high-density gold nanoparticles having target-orientedness are bound to protein surfaces, without an additional process of surface stabilization or process for providing target-orientedness. Compared with conventional gold nanoparticles, the gold-protein nanoparticle fusions according to the present invention show structural stability against pH variation and concentration variation, and also have excellent target-orientedness; therefore, the fusions can bring a dramatic enhancement to the utilization of gold nanoparticles in photothermal therapy.

公开(公告)号：US11878054B2

公开(公告)日：2024-01-23

申请号：US16988659

申请日：2020-08-09

申请人： Inventprise, LLC

发明人： Subhash V. Kapre

IPC分类号： A61K39/29 ,  A61K39/295 ,  A61K47/42 ,  C07K14/025 ,  A61K39/12 ,  A61K9/00 ,  A61K9/19 ,  A61K47/10 ,  C07K14/005 ,  C12N7/00 ,  A61K39/00

CPC分类号： A61K39/12 ,  A61K9/0019 ,  A61K9/19 ,  A61K47/10 ,  A61K47/42 ,  C07K14/005 ,  C12N7/00 ,  A61K2039/5258 ,  C12N2710/20034 ,  C12N2730/10123 ,  C12N2730/10142

摘要： The invention is directed to compositions and methods for the stabilization of viral and bacterial vaccines. Vaccines of the invention are contained in VLPs with stabilizing agents such as, for example, sugar alcohols (e.g., sorbitol) and degraded gelatins. Preferably the gelatin has an average molecular weight of 10,000 kilodaltons or less. These vaccines have a substantially improved thermostability as well as long term stability. The invention is also directed to the manufacture of a vaccine or the invention and methods for the administration of a vaccine of the invention to patients.

公开(公告)号：US20160122420A1

公开(公告)日：2016-05-05

申请号：US14896343

申请日：2014-06-05

申请人： David J. ROWLANDS ,  George LOMONOSSOFF ,  Hadrien PEYRET

发明人： David J. ROWLANDS ,  George LOMONOSSOFF ,  Hadrien PEYRET

IPC分类号： C07K16/18 ,  C12N7/00 ,  G01N33/68 ,  C07K14/005

CPC分类号： C07K16/18 ,  C07K14/005 ,  C07K14/02 ,  C07K16/00 ,  C07K2317/22 ,  C07K2317/569 ,  C07K2319/35 ,  C07K2319/735 ,  C07K2319/75 ,  C12N7/00 ,  C12N2730/10122 ,  C12N2730/10123 ,  C12N2730/10134 ,  C12N2730/10142 ,  C12N2730/10151 ,  G01N33/6857 ,  G01N2333/02

摘要： The invention provides a protein comprising a first and a second copy of hepatitis B core antigen (HBcAg) in tandem, in which one or both of the copies of HBcAg comprises a single-domain antibody fragment in the el loop.

摘要翻译： 本发明提供一种包含乙型肝炎核心抗原（HBcAg）的第一和第二拷贝的蛋白质，其中HBcAg的一个或两个拷贝在el环中包含单结构域抗体片段。

公开(公告)号：US20150132231A1

公开(公告)日：2015-05-14

申请号：US14533783

申请日：2014-11-05

申请人： The Board of Trustees of the Leland Stanford Junior University

发明人： Benjamin Yen-Bin Ko ,  James Robert Swartz

IPC分类号： A61K49/18 ,  C12N7/00

CPC分类号： C12N7/00 ,  A61K49/1839 ,  B82Y5/00 ,  B82Y40/00 ,  C12N2730/10123 ,  C12N2730/10142

摘要： Method and compositions are provided that relate to superparamagnetic nanoparticles, including IONP with small diameter and high transverse relaxivity, that are stable in an aqueous environment. The nanoparticle surface coating is functionalized, while minimizing its thickness, to facilitate packaging within biological constructs.

摘要翻译： 提供了涉及超顺磁性纳米颗粒的方法和组合物，其包括在水性环境中稳定的小直径和高横向弛豫度的IONP。 将纳米颗粒表面涂层功能化，同时使其厚度最小化，以促进生物构建体内的包装。

公开(公告)号：US20060210588A1

公开(公告)日：2006-09-21

申请号：US10550580

申请日：2004-03-25

申请人： Martin Bachmann ,  Adrian Huber ,  Vania Manolova ,  Edwin Meijerink ,  Karl Proba ,  Alain Tissot

发明人： Martin Bachmann ,  Adrian Huber ,  Vania Manolova ,  Edwin Meijerink ,  Karl Proba ,  Alain Tissot

IPC分类号： A61K39/21 ,  A61K48/00

CPC分类号： A61K39/21 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/5258 ,  A61K2039/55561 ,  C07K2319/40 ,  C12N7/00 ,  C12N2710/22023 ,  C12N2710/22042 ,  C12N2730/10122 ,  C12N2730/10123 ,  C12N2730/10142 ,  C12N2740/16034 ,  C12N2740/16222 ,  C12N2760/10022 ,  C12N2795/18122 ,  C12N2795/18123 ,  C12N2795/18142 ,  Y02A50/467

摘要： The present invention is related to the fields of molecular biology, virology, immunology and medicine. The invention provides a modified virus-like particle (VLP) comprising a VLP which can be loaded with immunostimulatory substances, in particular with DNA oligonucleotides containing non-methylated C and G (CpGs), and particular HIV peptides linked thereto. Such CpG-VLPs are dramatically more immunogenic that their CpG-free counterparts and induce enhanced B and T cell responses. The immune response against HIV peptides optionally coupled, fused or attached otherwise to the VLPs is similarly enhanced as the immune response against HIV peptides are especially directed to the Th1 type. Antigens attached to CpG-loaded VLPs may therefore be ideal vaccines for prophylactic or therapeutic vaccination against allergies, tumors and other self-molecules and chronic viral diseases.

摘要翻译： 本发明涉及分子生物学，病毒学，免疫学和医学领域。 本发明提供了包含VLP的修饰病毒样颗粒（VLP），其可加载免疫刺激物质，特别是与含有非甲基化C和G（CpG）的DNA寡核苷酸以及与其连接的特定HIV肽。 这样的CpG-VLP显着地更具免疫原性，使其不含CpG的对应物并诱导增强的B和T细胞应答。 任何偶联，融合或附加到VLP的HIV肽的免疫应答相似地增强，因为针对HIV肽的免疫应答特别针对Th1型。 因此，连接到CpG负载的VLP的抗原可能是针对过敏，肿瘤和其他自身分子和慢性病毒性疾病的预防性或治疗性接种疫苗的理想疫苗。

公开(公告)号：US20160303211A1

公开(公告)日：2016-10-20

申请号：US15201029

申请日：2016-07-01

申请人： AnGesMG, Inc.

发明人： Mariko KYUTOKU ,  Hironori NAKAGAMI ,  Hiroshi KORIYAMA ,  Futoshi NAKAGAMI ,  Ryuichi MORISHITA

IPC分类号： A61K39/00 ,  C12N7/00 ,  A61K39/29

CPC分类号： A61K39/0012 ,  A61K39/292 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/575 ,  A61K2039/58 ,  C12N7/00 ,  C12N15/85 ,  C12N2730/10142 ,  C12N2730/10143 ,  C12N2730/10171

摘要： The present invention provides an agent for the treatment or prophylaxis of arteriosclerosis comprising an expression vector encoding a chimeric Hepatitis B virus core antigen polypeptide inserted with an amino acid sequence containing a specific epitope of apolipoprotein (a), wherein the amino acid sequence containing the specific epitope is inserted between the amino acid residues 80 and 81 of the hepatitis B virus core antigen polypeptide.

摘要翻译： 本发明提供一种用于治疗或预防动脉硬化的药剂，其包含编码嵌入了含有载脂蛋白（a）的特异性表位的氨基酸序列的嵌合型乙型肝炎病毒核心抗原多肽的表达载体，其中所述氨基酸序列含有特异性 表位插入在乙型肝炎病毒核心抗原多肽的氨基酸残基80和81之间。

公开(公告)号：US20140086944A1

公开(公告)日：2014-03-27

申请号：US14032804

申请日：2013-09-20

申请人： AnGesMG, Inc.

发明人： Mariko KYUTOKU ,  Hironori NAKAGAMI ,  Hiroshi KORIYAMA ,  Futoshi NAKAGAMI ,  Ryuichi MORISHITA

IPC分类号： A61K39/00 ,  A61K39/29 ,  C12N15/85

CPC分类号： A61K39/0012 ,  A61K39/292 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/575 ,  A61K2039/58 ,  C12N7/00 ,  C12N15/85 ,  C12N2730/10142 ,  C12N2730/10143 ,  C12N2730/10171

摘要： The present invention provides an agent for the treatment or prophylaxis of arteriosclerosis comprising an expression vector encoding a chimeric Hepatitis B virus core antigen polypeptide inserted with an amino acid sequence containing a specific epitope of apolipoprotein (a), wherein the amino acid sequence containing the specific epitope is inserted between the amino acid residues 80 and 81 of the hepatitis B virus core antigen polypeptide.

摘要翻译： 本发明提供一种用于治疗或预防动脉硬化的药剂，其包含编码嵌入了含有载脂蛋白（a）的特异性表位的氨基酸序列的嵌合型乙型肝炎病毒核心抗原多肽的表达载体，其中所述氨基酸序列含有特异性 表位插入在乙型肝炎病毒核心抗原多肽的氨基酸残基80和81之间。

公开(公告)号：US20090155302A1

公开(公告)日：2009-06-18

申请号：US12201731

申请日：2008-08-29

申请人： Martin F. BACHMANN ,  Gary Jennings ,  Ivo Sonderegger

发明人： Martin F. BACHMANN ,  Gary Jennings ,  Ivo Sonderegger

IPC分类号： A61K39/12 ,  A61P37/08 ,  A61P31/12

CPC分类号： A61K39/0007 ,  A61K38/00 ,  A61K38/162 ,  A61K39/0005 ,  A61K39/0008 ,  A61K39/001 ,  A61K39/35 ,  A61K39/385 ,  A61K39/39 ,  A61K47/646 ,  A61K47/6901 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/6075 ,  A61K2039/627 ,  C07K14/005 ,  C07K14/523 ,  C07K14/5409 ,  C07K14/5437 ,  C07K16/00 ,  C07K16/082 ,  C07K16/22 ,  C07K16/2863 ,  C07K16/2875 ,  C07K16/40 ,  C07K16/4291 ,  C07K2317/34 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2319/00 ,  C07K2319/30 ,  C12N2730/10122 ,  C12N2730/10123 ,  C12N2730/10142 ,  C12N2795/18122 ,  C12N2810/852

摘要： The present invention is related to the fields of molecular biology, virology, immunology and medicine. The invention provides a composition comprising an ordered and repetitive antigen or antigenic determinant array, and in particular an array comprising a protein or peptide of IL-5, IL-13 or eotaxin. More specifically, the invention provides a composition comprising a virus-like particle and at least one protein, or peptide of IL-5, IL-13 and/or eotaxin bound thereto. The invention also provides a process for producing the conjugates and the ordered and repetitive arrays, respectively. The compositions of the invention are useful in the production of vaccines for the treatment of allergic diseases with an eosinophilic component and as a pharmaccine to prevent or cure allergic diseases with an eosinophilic component and to efficiently induce immune responses, in particular antibody responses. Furthermore, the compositions of the invention are particularly useful to efficiently induce self-specific immune responses within the indicated context.

摘要翻译： 本发明涉及分子生物学，病毒学，免疫学和医学领域。 本发明提供包含有序和重复抗原或抗原决定簇的组合物，特别是包含IL-5，IL-13或嗜酸细胞活化趋化因子的蛋白质或肽的阵列。 更具体地，本发明提供包含病毒样颗粒和与其结合的至少一种IL-5，IL-13和/或嗜酸性粒细胞趋化因子的蛋白质或肽的组合物。 本发明还提供了分别制备共轭物和有序和重复阵列的方法。 本发明的组合物可用于生产用于用嗜酸性成分治疗过敏性疾病的疫苗和作为药物以预防或治愈嗜酸性成分的过敏性疾病并有效诱导免疫应答，特别是抗体应答的疫苗。 此外，本发明的组合物特别有用于在指定的上下文中有效诱导自身特异性免疫应答。