Process for enzymatic ultrafiltration of deamidated protein
    2.
    发明授权
    Process for enzymatic ultrafiltration of deamidated protein 失效
    脱氨基蛋白的酶超滤方法

    公开(公告)号:US5250305A

    公开(公告)日:1993-10-05

    申请号:US544562

    申请日:1990-06-27

    申请人: Jamel S. Hamada

    发明人: Jamel S. Hamada

    摘要: This invention relates to methods for the large-scale enzymatic deamidation of foot proteins. Deamidating enzymes such as peptidoglutaminase are immobilized using a spiral membrane having a molecular weight cut-off, from about 3 to about 50 kd. The methods minimize loss of the enzymatic activity for subsequent reuse of the enzyme as well as increase deamidation product yield.

    摘要翻译: 本发明涉及足部蛋白质的大规模酶脱酰胺化方法。 使用分子量截留的约3至约50kd的螺旋膜固定脱酰氨基酶如肽聚谷氨酰胺酶。 该方法使酶活性的损失最小化以便随后重复使用酶以及增加脱酰胺产物产率。