公开(公告)号：US20120238867A1

公开(公告)日：2012-09-20

申请号：US13485412

申请日：2012-05-31

申请人： Dan HASHIMSHONY

发明人： Dan HASHIMSHONY

IPC分类号： A61B5/00 ,  A61B5/053 ,  A61B6/00

CPC分类号： A61K49/00 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/0091 ,  A61B5/0538 ,  A61B5/065 ,  A61B5/411 ,  A61B5/416 ,  Y10S607/901

摘要： A method, apparatus and probe for examining tissue for the presence of target cells, particularly cancerous cells, by subjecting the tissue to be examined to a contrast agent containing small particles of a physical element conjugated with a biological carrier selectively bindable to the target cells. Energy pulses are applied to the examined tissue. The changes in impedance and/or optical characteristics of the examined tissue produced by the applied energy pulses are detected and utilized for determining the presence of the target cells in the examined tissue. In a described preferred embodiment, the applied energy pulses include laser pulses, and the physical element conjugated with a biological carrier is a light-sensitive semiconductor having an impedance which substantially decreases in the presence of light. The same probe used for detecting the targeted cells may also be used for destroying the cells so targeted.

摘要翻译： 一种用于通过使检查组织对包含与选择性地与靶细胞结合的生物载体结合的物理元素的小颗粒的造影剂来检查组织是否存在靶细胞特别是癌细胞的方法，装置和探针。 能量脉冲被施加到被检查的组织。 检测由所施加的能量脉冲产生的被检查组织的阻抗和/或光学特性的变化并用于确定所检查组织中靶细胞的存在。 在所描述的优选实施例中，所施加的能量脉冲包括激光脉冲，并且与生物载体共轭的物理元件是具有在存在光时显着降低的阻抗的光敏半导体。 用于检测靶细胞的相同探针也可用于破坏如此靶向的细胞。

公开(公告)号：US20090187109A1

公开(公告)日：2009-07-23

申请号：US12334565

申请日：2008-12-15

申请人： Dan HASHIMSHONY

发明人： Dan HASHIMSHONY

IPC分类号： A61B6/00 ,  A61B5/053

CPC分类号： A61K49/00 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/0091 ,  A61B5/0538 ,  A61B5/065 ,  A61B5/411 ,  A61B5/416 ,  Y10S607/901

摘要： A method, apparatus and probe for examining tissue for the presence of target cells, particularly cancerous cells, by subjecting the tissue to be examined to a contrast agent containing small particles of a physical element conjugated with a biological carrier selectively bindable to the target cells. Energy pulses are applied to the examined tissue. The changes in impedance and/or optical characteristics of the examined tissue produced by the applied energy pulses are detected and utilized for determining the presence of the target cells in the examined tissue. In a described preferred embodiment, the applied energy pulses include laser pulses, and the physical element conjugated with a biological carrier is a light-sensitive semiconductor having an impedance which substantially decreases in the presence of light. The same probe used for detecting the targeted cells may also be used for destroying the cells so targeted.

摘要翻译： 一种用于通过使检查组织对包含与选择性地与靶细胞结合的生物载体结合的物理元素的小颗粒的造影剂来检查组织是否存在靶细胞特别是癌细胞的方法，装置和探针。 能量脉冲被施加到被检查的组织。 检测由所施加的能量脉冲产生的被检查组织的阻抗和/或光学特性的变化并用于确定所检查组织中靶细胞的存在。 在所描述的优选实施例中，所施加的能量脉冲包括激光脉冲，并且与生物载体共轭的物理元件是具有在存在光时显着降低的阻抗的光敏半导体。 用于检测靶细胞的相同探针也可用于破坏如此靶向的细胞。

公开(公告)号：US20060035304A1

公开(公告)日：2006-02-16

申请号：US11157478

申请日：2005-06-20

申请人： Carlito Lebrilla ,  Hyun An ,  Kit Lam ,  Suzanne Miyamoto

发明人： Carlito Lebrilla ,  Hyun An ,  Kit Lam ,  Suzanne Miyamoto

IPC分类号： C12Q1/34

CPC分类号： G01N33/57415 ,  G01N33/57449 ,  G01N33/66 ,  Y10S607/901

摘要： The present invention provides methods for identifying oligosaccharides specific to cancer and methods for determining a strain of cancer in an individual. The present invention also provides methods for diagnosing cancer or a stage of cancer in an individual by detecting the presence or absence of specific cancer markers and methods for treating cancer by administering antibodies directed to such markers. In addition, the present invention provides cancer markers comprising O-linked oligosaccharides and kits for diagnosing or treating cancer.

摘要翻译： 本发明提供用于鉴定癌症特异性的寡糖的方法和用于确定个体中癌症菌株的方法。 本发明还提供了通过检测针对这种标记的抗体来检测特异性癌症标志物的存在或不存在以及用于治疗癌症的方法来诊断个体癌症或癌症阶段的方法。 此外，本发明提供包含O-连接的寡糖的癌症标志物和用于诊断或治疗癌症的试剂盒。

公开(公告)号：US5028621A

公开(公告)日：1991-07-02

申请号：US352774

申请日：1989-05-16

申请人： Thomas J. Dougherty ,  William R. Potter ,  Kenneth R. Weishaupt

发明人： Thomas J. Dougherty ,  William R. Potter ,  Kenneth R. Weishaupt

IPC分类号： A61B5/00 ,  A61K31/40 ,  A61K41/00 ,  A61K49/00 ,  A61K51/00 ,  A61N5/06 ,  A61P35/00 ,  A61P43/00 ,  C07D487/22 ,  C07D519/00 ,  C09B47/00 ,  G02B6/04 ,  G02B6/26 ,  G02B6/42

CPC分类号： A61K31/40 ,  A61B5/411 ,  A61B5/416 ,  A61K41/0071 ,  A61N5/0601 ,  A61N5/062 ,  C07D519/00 ,  G02B6/262 ,  G02B6/4204 ,  A61B5/0071 ,  A61B5/0084 ,  G02B6/4298 ,  Y10S607/901

摘要： To obtain tumor-selective, photosensitizing drugs useful in the localization of neoplastic tissue and treatment of abnormal neoplastic tissue such as tumors, one of two methods is used. In the first method, a hydrolyzed mixture of the products of reaction of hematoporphyrin with acetic acid and sulfuric acid is cycled through a microporous membrane system to exclude low molecular weight products. In the second method, drugs are synthesized or derived from other pyrrole compounds. The drugs (1) include two covalently bound groups, each with four rings, some of which are pyrroles such as phlorins, porphyrins, chlorins, substituted pyrroles, substituted chlorins or substituted phlorins, each group being arranged in a ring structure, connected covalently to another group and have a triplet energy state above 37.5 kilocalories per mole; (2) are soluble in water, forming an aggregate of over 10,000 molecular weight in water and have an affinity for each other compared to serum protein such that 10 to 100 percent remain self aggregated in serum protein; and (3) are lipophyllic and able to disaggregate and attach to cell plasma, nuclear membrane, mitochondria, lysosomes and tissue. The drug obtained by the first method has an empirical formula of approximately C.sub.68 H.sub.70 N.sub.8 O.sub.11 or C.sub.68 H.sub.66 N.sub.8 O.sub.11 Na.sub.4. Neoplastic tissue retains the drug after it has cleared normal tissues and illumination results in necrosis. Moreover, other photosensitizing materials may be combined with a carrier that enters undesirable tissues and cells of the reticular endothelial system such as macrophages. These photosensitizing materials: (1) must have a triplet energy state above 3.5 kilocalories per mole; (2) cannot be easily oxidized; and (3) not physically quench any required energy state. Preferably, this photosensitizing material should be lipophlic.

摘要翻译： 为了获得肿瘤选择性的光敏药物，可用于肿瘤组织的定位和治疗异常肿瘤组织如肿瘤，使用两种方法之一。 在第一种方法中，血卟啉与乙酸和硫酸的反应产物的水解混合物循环通过微孔膜系统以排除低分子量产物。 在第二种方法中，合成或衍生自其他吡咯化合物的药物。 药物（1）包括两个共价结合的基团，每个具有四个环，其中一些是吡咯，例如卟啉，卟啉，二氢卟酚，取代的吡咯，取代的二氢卟酚或取代的藜芦烯，每个组排列成环结构，共价连接到 另一组，三重态能量每摩尔高于37.5千卡; （2）可溶于水，与血清蛋白质相比，在水中形成了超过10,000分子量的聚集体，并且与血清蛋白质具有亲和力，使得10％至100％在血清蛋白中保持自我聚集; 和（3）是脂蛋白的并且能够解聚并附着于细胞血浆，核膜，线粒体，溶酶体和组织。 通过第一种方法获得的药物具有约C68H70N8O11或C68H66N8O11Na4的经验式。 肿瘤组织清除正常组织后保留药物，照明会导致坏死。 此外，其它光敏材料可以与进入网状内皮系统的不希望的组织和细胞如巨噬细胞的载体组合。 这些光敏材料：（1）必须具有每千克3.5千卡以上的三线态能量状态; （2）不易氧化; 和（3）不能物理地淬灭任何所需的能量状态。 优选地，该光敏材料应该是脂肪族的。

公开(公告)号：US4649151A

公开(公告)日：1987-03-10

申请号：US609991

申请日：1984-05-14

申请人： Thomas J. Dougherty ,  William R. Potter ,  Kenneth R. Weishaupt

发明人： Thomas J. Dougherty ,  William R. Potter ,  Kenneth R. Weishaupt

IPC分类号： A61B5/00 ,  A61K31/40 ,  A61K41/00 ,  A61K49/00 ,  A61K51/00 ,  A61N5/06 ,  A61P35/00 ,  A61P43/00 ,  C07D487/22 ,  C07D519/00 ,  C09B47/00 ,  G02B6/04 ,  G02B6/26 ,  G02B6/42

CPC分类号： A61K31/40 ,  A61B5/411 ,  A61B5/416 ,  A61K41/0071 ,  A61N5/0601 ,  A61N5/062 ,  C07D519/00 ,  G02B6/262 ,  G02B6/4204 ,  A61B5/0071 ,  A61B5/0084 ,  G02B6/4298 ,  Y10S607/901

摘要： To obtain tumor-selective, photosensitizing drugs useful in the localization of neoplastic tissue and treatment of abnormal neoplastic tissue such as tumors, one of two methods is used. In the first method, a hydrolyzed mixture of the products of reaction of hematoporphyrin with acetic acid and sulfuric acid is cycled through a microporous membrane system to exclude low molecular weight products. In the second method, drugs are synthesized or derived from other pyrrole compounds. The drugs: (1) include two covalently bound groups, each with four rings, some of which are pyrroles such as phlorins, porphyrins, chlorins, substituted pyrroles, substituted chlorins or substituted phlorins, each group being arranged in a ring structure, connected covalently to another group and have a triplet energy state above 37.5 kilocalories per mole; (2) are soluble in water, forming an aggregate of over 10,000 molecular weight in water and have an affinity for each other compared to serum protein such that 10 to 100 percent remain self aggregated in serum protein; and (3) are lipophyllic and able to disaggregate and attach to cell plasma, nuclear membrane, mitochondria, lysosomes and tissue. The drug obtained by the first method has an empirical formula of approximately C.sub.68 H.sub.70 N.sub.8 O.sub.11 or C.sub.68 H.sub.66 N.sub.8 O.sub.11 Na.sub.4.

摘要翻译： 为了获得肿瘤选择性的光敏药物，可用于肿瘤组织的定位和治疗异常肿瘤组织如肿瘤，使用两种方法之一。 在第一种方法中，血卟啉与乙酸和硫酸的反应产物的水解混合物循环通过微孔膜系统以排除低分子量产物。 在第二种方法中，合成或衍生自其他吡咯化合物的药物。 药物：（1）包括两个共价结合的基团，每个具有四个环，其中一些是吡咯，例如卟啉，卟啉，二氢卟酚，取代的吡咯，取代的二氢卟酚或取代的藜芦烯，每个组排列成环结构，共价连接 到另一组，并具有高于每摩尔37.5千卡的三线态能量状态; （2）可溶于水，与血清蛋白质相比，在水中形成了超过10,000分子量的聚集体，并且与血清蛋白质具有亲和力，使得10％至100％在血清蛋白中保持自我聚集; 和（3）是脂蛋白的并且能够解聚并附着于细胞血浆，核膜，线粒体，溶酶体和组织。 通过第一种方法获得的药物具有约C68H70N8O11或C68H66N8O11Na4的经验式。

公开(公告)号：US09226979B2

公开(公告)日：2016-01-05

申请号：US13485412

申请日：2012-05-31

申请人： Dan Hashimshony

发明人： Dan Hashimshony

IPC分类号： A61B5/00 ,  A61K49/00 ,  A61B5/053 ,  A61B5/06

CPC分类号： A61K49/00 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/0091 ,  A61B5/0538 ,  A61B5/065 ,  A61B5/411 ,  A61B5/416 ,  Y10S607/901

摘要： A method, apparatus and probe for examining tissue for the presence of target cells, particularly cancerous cells, by subjecting the tissue to be examined to a contrast agent containing small particles of a physical element conjugated with a biological carrier selectively bindable to the target cells. Energy pulses are applied to the examined tissue. The changes in impedance and/or optical characteristics of the examined tissue produced by the applied energy pulses are detected and utilized for determining the presence of the target cells in the examined tissue. In a described preferred embodiment, the applied energy pulses include laser pulses, and the physical element conjugated with a biological carrier is a light-sensitive semiconductor having an impedance which substantially decreases in the presence of light. The same probe used for detecting the targeted cells may also be used for destroying the cells so targeted.

公开(公告)号：US07651847B2

公开(公告)日：2010-01-26

申请号：US11157478

申请日：2005-06-20

申请人： Carlito B. Lebrilla ,  Hyun Joo An ,  Kit S. Lam ,  Suzanne Miyamoto

发明人： Carlito B. Lebrilla ,  Hyun Joo An ,  Kit S. Lam ,  Suzanne Miyamoto

IPC分类号： C12Q1/34 ,  C12Q1/02 ,  C12N5/00 ,  C12N5/02 ,  C12N5/08 ,  C12N5/16 ,  C12N5/22

CPC分类号： G01N33/57415 ,  G01N33/57449 ,  G01N33/66 ,  Y10S607/901

摘要： The present invention provides methods for identifying oligosaccharides specific to cancer and methods for determining a strain of cancer in an individual. The present invention also provides methods for diagnosing cancer or a stage of cancer in an individual by detecting the presence or absence of specific cancer markers and methods for treating cancer by administering antibodies directed to such markers. In addition, the present invention provides cancer markers comprising O-linked oligosaccharides and kits for diagnosing or treating cancer.

摘要翻译： 本发明提供用于鉴定癌症特异性的寡糖的方法和用于确定个体中癌症菌株的方法。 本发明还提供了通过检测针对这种标记的抗体来检测特异性癌症标志物的存在或不存在以及用于治疗癌症的方法来诊断个体癌症或癌症阶段的方法。 此外，本发明提供包含O-连接的寡糖的癌症标志物和用于诊断或治疗癌症的试剂盒。

公开(公告)号：US6061591A

公开(公告)日：2000-05-09

申请号：US824649

申请日：1997-03-24

申请人： Lutz Freitag ,  Franz Dankwart-Eder

发明人： Lutz Freitag ,  Franz Dankwart-Eder

IPC分类号： A61B10/00 ,  A61B1/00 ,  A61B1/06 ,  A61B5/00

CPC分类号： A61B5/0084 ,  A61B1/00186 ,  A61B1/043 ,  A61B1/0638 ,  A61B1/0646 ,  A61B5/0071 ,  Y10S607/901

摘要： The invention relates to an arrangement and method for diagnosing malignant tissue by fluorescence observation with an endoscope. A stroboscope is used as a white light source in order to illuminate the interesting tissue region through the endosocope. A laser is simultaneously used in order to stimulate the fluorescence. At a time, the analyzer is activated in the dark phase between two light flashes of the stroboscope and deactivated during a visible light flash. The tissue, with a continuous fluorescence stimulation by exposure to light with a stimulating wavelength, may be observed under a pseudo-white light. The spectrogram may be represented on a screen which displays a normal white light endoscopic picture.

摘要翻译： 本发明涉及一种通过内窥镜进行荧光观察来诊断恶性组织的装置和方法。 频闪仪用作白光源，以通过内窥镜照射感兴趣的组织区域。 同时使用激光来刺激荧光。 一次，分析仪在闪光阶段的两个闪光之间的暗相中被激活，并在可见光闪光期间被去激活。 在伪白光下可以观察到具有通过暴露于具有刺激波长的光的连续荧光刺激的组织。 光谱图可以在显示正常的白光内窥镜图像的屏幕上表示。

公开(公告)号：US5775339A

公开(公告)日：1998-07-07

申请号：US914272

申请日：1997-08-19

申请人： Kathryn W. Woodburn ,  Qing Fan ,  Stuart W. Young

发明人： Kathryn W. Woodburn ,  Qing Fan ,  Stuart W. Young

IPC分类号： A61K31/407 ,  A61K41/00 ,  A61B19/00

CPC分类号： A61K31/407 ,  A61K41/0033 ,  A61K41/0076 ,  Y10S607/901

摘要： The present invention involves the use of a photosensitive texaphyrin for the photodynamic treatment of a pigmented lesion, such as a melanodermic lesion, or a lesion obscured by pigmented tissue such as melaniferous tissue in a subject. In particular, the invention provides a treatment for the metastatic process of melanoma, and a method for inhibiting growth of established metastases of melanoma. The photosensitive texaphyrin may be a free-base texaphyrin or may be metallated with a diamagnetic metal. Preferably, the texaphyrin is metallated with lutetium. Heretofore, melanoma has been refractory to treatment with photodynamic therapy.

摘要翻译： 本发明涉及使用感光性泰克萨菲瑞用于色素沉着病变如黑素瘤病变的光动力学治疗或被受试者中着色组织如黑色素组织遮盖的病变。 特别地，本发明提供了黑素瘤的转移过程的治疗方法，以及抑制已有的黑色素瘤转移的生长的方法。 感光性泰克萨菲瑞可以是游离碱泰克萨菲瑞，也可以用抗磁性金属进行金属化。 泰克萨菲瑞优选用镥金属化。 迄今为止，黑素瘤已经用光动力疗法治疗难以治愈。

公开(公告)号：US5560357A

公开(公告)日：1996-10-01

申请号：US379994

申请日：1995-01-27

申请人： Mark L. Faupel ,  Steven Hahn, deceased

发明人： Mark L. Faupel ,  Steven Hahn, deceased

IPC分类号： A61B5/04 ,  A61B5/0408 ,  A61B5/05 ,  A61B5/00

CPC分类号： A61B5/04 ,  A61B5/04002 ,  A61B5/0408 ,  A61B5/04087 ,  A61B5/05 ,  A61B2562/0238 ,  A61B2562/08 ,  Y10S607/901

摘要： The apparatus and method for screening and diagnosing trauma or disease in body tissues involves the use of DC biopotential sensing electrodes having DC offset potentials within an acceptable range and electrical characteristics which enhance the accuracy of the biopotential measurement. The apparatus checks the electrodes connected thereto prior to a measurement cycle and will not initiate the measurement cycle if improper electrodes are present. The electrodes may be formed to selectively pass only certain ions thereby enhancing the sensitivity of the electrodes.

摘要翻译： 用于筛选和诊断身体组织中的创伤或疾病的装置和方法涉及使用具有在可接受范围内的DC偏移电位的DC生物电势感测电极和提高生物电位测量的精度的电特性。 该装置在测量周期之前检查连接到其上的电极，如果存在不正确的电极，则不会启动测量周期。 可以形成电极以选择性地仅通过某些离子，从而增强电极的灵敏度。