公开(公告)号：US20240271166A1

公开(公告)日：2024-08-15

申请号：US18355776

申请日：2023-07-20

申请人： LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC

发明人： Aaron ARGYROS ,  William R. KENEALY ,  Emily STONEHOUSE

IPC分类号： C12P7/06 ,  C07K14/395 ,  C12N1/22 ,  C12N1/32 ,  C12N9/04 ,  C12N15/81 ,  C12P7/04

CPC分类号： C12P7/06 ,  C07K14/395 ,  C12N1/22 ,  C12N1/32 ,  C12N9/0006 ,  C12N15/81 ,  C12P7/04 ,  C12Y101/01008 ,  Y02E50/10

摘要： The present invention provides for novel metabolic pathways to reduce or modulate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous proteins that function to import glycerol and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and/or heterologous proteins or enzymes is activated, upregulated, or downregulated. The invention also provides for a recombinant microorganism comprising one or more native or heterologous proteins that function to regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source to ethanol, wherein said one or more native and/or heterologous proteins or enzymes is activated, upregulated or downregulated. Also provided are methods for increasing cellular glycerol uptake and increasing recombinant production of fuels and other chemicals using the recombinant microorganisms of the invention.

公开(公告)号：US20230356144A1

公开(公告)日：2023-11-09

申请号：US18312893

申请日：2023-05-05

申请人： CEMVITA FACTORY, INC.

发明人： Roger Allen HARRIS ,  Rajesh GUPTA ,  Kevin Patrick KINCAID ,  Tahereh KARIMI ,  Matthew SHAPIRO ,  Nymul KHAN ,  Marcio Luis Busi DA SILVA ,  Edris TAHER

IPC分类号： B01D53/18 ,  B01D53/14 ,  C12N1/20 ,  C12M1/00 ,  C12M1/34 ,  C12N1/32

CPC分类号： B01D53/1475 ,  B01D53/1412 ,  B01D53/1425 ,  B01D53/18 ,  C12M23/58 ,  C12M29/08 ,  C12M41/12 ,  C12M41/26 ,  C12M41/34 ,  C12N1/20 ,  C12N1/32 ,  B01D2252/60

摘要： The present application provides a process for sequestering carbon dioxide to produce a biomass containing reaction product, the process comprising the steps of:

I. contacting a raw carbon dioxide-containing feedstock with an absorption or dissolution medium to form a reagent stream comprising dissolved or absorbed inorganic carbon at least in the form of HCO3- and CO32- wherein the HCO3- :CO32- molar ratio in the reagent stream is at least about 0.8;
II. contacting at least a portion of the reagent stream with a microbial broth in a bioreactor to produce a biomass-containing reaction product;
III. separating the biomass-containing reaction product into a biomass product and a liquid stream; and
IV. recycling at least a portion of the liquid stream to step i. of the process for use as, or as part of, the absorption or dissolution medium,

wherein the pH of the absorption or dissolution medium is controlled to maintain the HCO3- :CO32- molar ratio in the reagent stream at least about 0.8.

公开(公告)号：US20220411837A1

公开(公告)日：2022-12-29

申请号：US17632363

申请日：2020-08-03

申请人： STRING BIO PRIVATE LIMITED

发明人： Rajeev S. KUMAR ,  Vinita LUKOSE ,  Shruthi S ,  Ezhilkani SUBBIAN ,  Shirish GAJANAN GOLE ,  Deepika SINGH

IPC分类号： C12P17/16 ,  C12N15/52 ,  C12N1/20 ,  C12N1/32

摘要： The instant disclosure is in the field of biosciences, more particularly towards molecular and industrial biotechnology. The present disclosure relates to recombinant methanotrophic bacteria capable of synthesizing indigo from methane, a method of developing said recombinant methanotrophic bacteria, and a method of indigo biosynthesis by the recombinant methanotrophic bacteria in presence of a methane source.

公开(公告)号：US20220135929A1

公开(公告)日：2022-05-05

申请号：US17141600

申请日：2021-01-05

申请人： Tongji University

发明人： Xiaohu DAI ,  Lei LI ,  Jun GAO ,  Bin DONG ,  Lingling DAI

IPC分类号： C12M1/00 ,  C12N1/32 ,  C12P1/04 ,  C12P7/62

摘要： Anaerobic digestion is enhanced based on the coupling of electron transfer with microbial electrolytic cell. A traditional anaerobic digestion reactor is used as the main body, a microbial electrolytic cell applied with a micro voltage is constructed, and the electron transfer in the system is optimized by an immobilized conductor material, to establish an efficient electron output-transfer-consumption anaerobic digestion pathway to produce methane.

公开(公告)号：US20220002764A1

公开(公告)日：2022-01-06

申请号：US17293230

申请日：2019-11-14

申请人： Manus Bio Inc.

发明人： Ryan A. PHILIPPE ,  Ajikumar Parayil KUMARAN ,  Christine Nicole S. SANTOS ,  Lu CHEN

IPC分类号： C12P7/42 ,  C12N9/12 ,  C12N15/52 ,  C12N1/16 ,  C12N1/32 ,  C12N1/20 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N9/00

摘要： Enzymes involved in cannabinoid biosynthesis are recombinantly expressed in a host cell. The host cell may be a prokaryote (e.g. Escherichia coli) or a eukaryote (e.g. Yarrowia lipolytica). The enzymes include a heterologous cannabigerolic acid synthase as well as additional enzymes involved in the biosynthesis of cannabinoid precursors such as geranyl diphosphate, olivetol, olivetolic acid, divarin and/or divarinic acid. Methods are provided for producing C5-cannabinoids and/or C3-cannabinoids by fermentation of the recombinant host cell. Alternatively, cannabinoids can be produced by biotransformation of cannabinoid precursors in recombinant cells or by disrupted recombinant cells.

公开(公告)号：US20210309959A1

公开(公告)日：2021-10-07

申请号：US17354218

申请日：2021-06-22

申请人： BioSherpa, LLC

发明人： Thomas A. Cumbo ,  Christopher L. Hutson ,  Destin J. Radder

IPC分类号： C12N1/04 ,  C12N1/32 ,  C12N1/20

摘要： The present application relates to systems and methods for transporting biological samples, including a transport medium configured to protect bacterial viability and optimize sample quality, for enhanced diagnostic accuracy. The disclosed systems and methods can include a transport media, a collection tube including a screen and sampling brush, and a transport case. The disclosed systems and methods allow samples to be frozen and thawed while preserving cellular wall integrity during transport or storage, and furthermore can preserve the survival of the bacterial sample, enabling more accurate detection of an infectious agent.

公开(公告)号：US10851398B2

公开(公告)日：2020-12-01

申请号：US16145196

申请日：2018-09-28

申请人： Ikeda Food Research Co., Ltd. ,  PHC Corporation

发明人： Hironori Omura ,  Hirokazu Sanada ,  Takako Yada ,  Ayaka Atsumi ,  Tetsunari Morita ,  Emi Ishimaru

IPC分类号： C12Q1/00 ,  C12N9/02 ,  C12N1/32 ,  C12N9/04 ,  G01N27/327 ,  C12Q1/32

摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.

公开(公告)号：US20190078120A1

公开(公告)日：2019-03-14

申请号：US16080928

申请日：2017-03-03

申请人： NIIGATA UNIVERSITY ,  ADEKA CORPORATION

发明人： Tsutomu SATO ,  Kotone OKUNO ,  Toshihiko TAKEHANA ,  Seiji KOIKE

IPC分类号： C12P7/04 ,  C12N9/90 ,  C12N1/32 ,  C12N1/20 ,  C12N9/88 ,  C12N9/04

摘要： An object of the present invention is to provide a method for preparing ambrein, which can easily obtain the ambrein. The object can be solved by a mutated tetraprenyl-β-curcumene cyclase wherein a fourth amino acid residue of a DXDD motif, aspartic acid, is substituted with an amino acid other than aspartic acid, (a) having a QXXXGX(W/F) motif, and a QXXXX(G/A)X(F/W/Y) motif on the N-terminal side, and a QXXXGX(F/W/Y) motif, and a QXXXGXW motif and a QXXXGX(F/W) motif on the C-terminal side, and not having a QXXXGXW motif at a position separated by 170 amino acid residues or more on the C-terminal side, with respect to the DXDD motif, (b) having 40% or more identity with the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 13, (c) exhibiting ambrein production activity using squalene as a substrate.

公开(公告)号：US10017729B2

公开(公告)日：2018-07-10

申请号：US15164062

申请日：2016-05-25

申请人： CHR. HANSEN A/S

发明人： Henrik Møllgaard ,  Henrik Rømer ,  Asger Geppel

IPC分类号： C12N1/20 ,  C12N1/26 ,  C12N1/32

CPC分类号： C12N1/20 ,  C12N1/26 ,  C12N1/32

摘要： This invention relates to an improved process for culturing bacteria of the family Streptococcaceae (such as of the genus lactococcus), a medium for culturing the bacteria, and the obtained bacteria cells.

公开(公告)号：US20170173087A1

公开(公告)日：2017-06-22

申请号：US15129611

申请日：2015-03-27

申请人： CONJUGON, INC.

发明人： Steven R. Watt ,  Caleb W. Dorsey ,  Joshua A. Smith

IPC分类号： A61K35/741 ,  C12N1/20 ,  A61K9/00 ,  C12N1/32 ,  A61M25/00 ,  A61L29/14

CPC分类号： A61K35/741 ,  A61K9/0034 ,  A61K35/74 ,  A61L29/005 ,  A61L29/085 ,  A61L29/145 ,  A61L2400/10 ,  A61M25/0017 ,  A61M25/0045 ,  A61M2025/0046 ,  C12N1/20 ,  C12N1/32 ,  C12N1/38 ,  C12N2500/05 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/60 ,  Y02A50/473

摘要： The invention relates to methods of differentiation, isolation, propagation, and storage of small colony variants (SCVs) of E. coli, preferably E. coli 83972 or E. coli HU2117, or modified or variant forms thereof, and methods for using the pre-pared SCV bacteria to establish probiotic biofilms in treated subjects and/or on treated medical devices.