公开(公告)号：US12234497B2

公开(公告)日：2025-02-25

申请号：US18104500

申请日：2023-02-01

Applicant: Kiverdi, Inc.

Inventor： John S. Reed ,  Lisa Dyson

IPC: C12P7/625 ,  C12M1/00 ,  C12N1/12 ,  C12N1/20 ,  C12P1/04 ,  C12P3/00 ,  C12P5/02 ,  C12P7/06 ,  C12P7/08 ,  C12P7/16 ,  C12P7/40 ,  C12P7/54 ,  C12P7/6463 ,  C12P7/649 ,  C12P21/00 ,  C25B1/04 ,  C25B15/02

Abstract: Compositions and methods for a hybrid biological and chemical process that captures and converts carbon dioxide and/or other forms of inorganic carbon and/or CI carbon sources including but not limited to carbon monoxide, methane, methanol, formate, or formic acid, and/or mixtures containing CI chemicals including but not limited to various syngas compositions, into organic chemicals including biofuels or other valuable biomass, chemical, industrial, or pharmaceutical products are provided. The present invention, in certain embodiments, fixes inorganic carbon or CI carbon sources into longer carbon chain organic chemicals by utilizing microorganisms capable of performing the oxyhydrogen reaction and the autotrophic fixation of CO2 in one or more steps of the process.

公开(公告)号：US12234463B2

公开(公告)日：2025-02-25

申请号：US17255352

申请日：2019-06-26

Applicant: Genomatica, Inc.

Inventor： Harish Nagarajan ,  Tae Hoon Yang ,  Ali Khodayari

IPC: C12N9/16 ,  C12N9/02 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P5/00 ,  C12P5/02 ,  C12P7/16 ,  C12P7/18 ,  C12P7/24 ,  C12P7/42 ,  C12P7/46 ,  C12P7/6409 ,  C12P13/00

Abstract: Described herein are engineered cells including ones having synthetic methylotrophy which include an NADH-dependent enzyme capable of converting G3P to 3PG (e.g., B. methanolicus gapN) and/or fructose-1,6-bisphosphatase, along with hexulose-6-phosphate synthase, 6-phospho-3-hexuloisomerase, a phosphoketolase, or a combination thereof. Engineered cells of the disclosure beneficially maintain adequate pool sizes of phosphorylated C3 and/or C4 compounds, and/or provide increased levels of NADPH. As such, the modifications allow for the generation of C6 compounds from C1 (e.g., a methanol feedstod) and C5 compounds, the regeneration of C5 compounds from C6 compounds by carbon rearrangement, and an improved balance between regeneration of C5 compounds and lower glycolysis. In turn, this allows the engineered microorganism to generate sufficient quantities of metabolic precursors (e.g., acetyl-CoA) which can be used in a bioproduct pathway, and the engineered cells can include further modifications to those pathway enzymes allowing for production of a desired bioproduct.

公开(公告)号：US12188074B2

公开(公告)日：2025-01-07

申请号：US17030914

申请日：2020-09-24

Applicant: Saudi Arabian Oil Company

Inventor： Wei Wang ,  Hooisweng Ow ,  Sehoon Chang

IPC: C12P7/16 ,  B82Y40/00 ,  C01B33/12 ,  C01C1/08 ,  C08G61/12 ,  C09K8/524 ,  C09K8/84 ,  C09K8/86 ,  E21B43/241

Abstract: A method for reducing condensate in a subsurface formation is disclosed. The method includes introducing a reactive mixture including an aqueous solution, urea, dopamine, a silica nanoparticle precursor, a silane grafting compound, and an alcohol compound into the subsurface formation. The method also includes allowing generation of ammonia through thermal decomposition of the urea and allowing the silica nanoparticle precursor to hydrolyze, thereby forming silica nanoparticles. The method further includes allowing the silane grafting compound to graft onto the silica nanoparticles, thereby forming functionalized silica nanoparticles. The method also includes allowing polymerization of the dopamine, thereby forming polydopamine. The method also includes allowing the functionalized silica nanoparticles to attach to the subsurface formation via the polydopamine, thereby reducing condensate in the subsurface formation.

公开(公告)号：US12188024B2

公开(公告)日：2025-01-07

申请号：US18316222

申请日：2023-05-11

Applicant: Utilization of Carbon Dioxide Institute Co., Ltd. ,  The University of Tokyo

Inventor： Hideaki Yukawa ,  Naoto Ohtani ,  Masaharu Ishii

IPC: C12N9/88 ,  C12N9/04 ,  C12N15/74 ,  C12P7/06 ,  C12P7/16

Abstract: A transformant obtained by introducing a DNA of (a1), (a2), or (a3) below, and (b) an alcohol dehydrogenase gene, into a bacterium of the genus Hydrogenophilus, can efficiently produce isobutanol utilizing carbon dioxide as a sole carbon source. (a1) DNA which consists of a base sequence of SEQ ID NO: 1; (a2) DNA which consists of a base sequence having 90% or more identity with SEQ ID NO: 1, the DNA encoding a polypeptide having 2-keto-acid decarboxylase activity; (a3) DNA which hybridizes with a DNA consisting of a base sequence complementary to SEQ ID NO: 1 under stringent conditions, and which encodes a polypeptide having 2-keto-acid decarboxylase activity.

公开(公告)号：US20250002949A1

公开(公告)日：2025-01-02

申请号：US18594617

申请日：2024-03-04

Applicant: Sasya Inc.

Inventor： Goutham Vemuri ,  Christopher Lindsay ,  Kevin Roberg-Perez ,  Christopher D. Snow ,  Elizabeth A. Cameron

IPC: C12P7/16 ,  C12N1/20 ,  C12N9/04 ,  C12N9/88 ,  C12N15/52

Abstract: The biological production of beta-hydroxyisovalerate (βHIV) using a non-natural microorganism. The non-natural microorganism for the biologically-derived βHIV provides more beta-hydroxyisovalerate synthase activity than the wild-type parent. The non-natural microorganism can host a non-natural enzyme, such as the non-natural enzyme expressed in a yeast or bacteria, wherein the non-natural microorganism comprises an active βHIV metabolic pathway for the production of βHIV. The biological derivation of βHIV eliminates toxic by-products and impurities that result from the chemical production of βHIV, such that βHIV produced by a non-natural microorganism prior to any isolation or purification process has not been in substantial contact with any halogen-containing component.

公开(公告)号：US12146132B2

公开(公告)日：2024-11-19

申请号：US18626055

申请日：2024-04-03

Applicant: Hubei University

Inventor： Shihui Yang ,  Mimi Hu ,  Qiaoning He

IPC: C12N1/20 ,  C12P7/06 ,  C12P7/16 ,  C12P7/42 ,  C12R1/01

Abstract: Genetically modified Zymomonas mobilis to utilize inorganic N source, methods and uses thereof are provided. The genetically modified Z. mobilis has a genome with the knockout of a locus ZMO1107 from Z. mobilis ZM4 could ferment inorganic N source.

公开(公告)号：US20240368638A1

公开(公告)日：2024-11-07

申请号：US18379079

申请日：2023-10-11

Applicant: PRECIGEN, INC.

Inventor： William J. COLEMAN ,  Genevieve M. VIDANES ,  Guillaume COTTAREL ,  Sheela MULEY ,  Roy KAMIMURA ,  Akbar F. JAVAN ,  Jianping SUN ,  Eli S. GROBAN

IPC: C12P7/16 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/52

Abstract: Multi-carbon compounds such as ethanol, n-butanol, sec-butanol, isobutanol, tert-butanol, fatty (or aliphatic long chain) alcohols, fatty acid methyl esters, 2,3-butanediol and the like, are important industrial commodity chemicals with a variety of applications. The present invention provides metabolically engineered host microorganisms which metabolize methane (CH4) as their sole carbon source to produce multi-carbon compounds for use in fuels (e.g., bio-fuel, bio-diesel) and bio-based chemicals. Furthermore, use of the metabolically engineered host microorganisms of the invention (which utilize methane as the sole carbon source) mitigate current industry practices and methods of producing multi-carbon compounds from petroleum or petroleum-derived feedstocks, and ameliorate much of the ongoing depletion of arable food source “farmland” currently being diverted to grow bio-fuel feedstocks, and as such, improve the environmental footprint of future bio-fuel, bio-diesel and bio-based chemical compositions.

公开(公告)号：US12071637B2

公开(公告)日：2024-08-27

申请号：US17740550

申请日：2022-05-10

Applicant: National University of Singapore

Inventor： Kun-Lin Yang ,  Kang Zhou ,  Yonghao Cui

IPC: C12N9/04 ,  C12N1/21 ,  C12N9/20 ,  C12P7/04 ,  C12P7/16

CPC classification number: C12N9/0006 ,  C12N9/20 ,  C12P7/04 ,  C12P7/16

Abstract: The present invention relates to a composition or combination for the production of butanol and isopropanol, comprising an acetone-butanol-ethanol (ABE)-producing Clostridium strain and a genetically engineered B. subtilis strain, wherein said genetically engineered B. subtilis strain has been transformed by at least one polynucleotide molecule; the at least one polynucleotide molecule comprising a secondary alcohol dehydrogenase gene operably linked to at least one promoter. The invention also relates to methods of producing butanol and isopropanol in a co-culture, methods of producing butyrate, isopropanol and butanol in a co-culture and methods of producing esters.

公开(公告)号：US20240279690A1

公开(公告)日：2024-08-22

申请号：US18444307

申请日：2024-02-16

Applicant: Filament Biosciences, LLC.

Inventor： Marcella Roe

IPC: C12P7/16 ,  C12P7/04 ,  C12P17/06 ,  C12P17/10

CPC classification number: C12P7/16 ,  C12P7/04 ,  C12P17/06 ,  C12P17/10

Abstract: A chemical extraction method comprising exposing a biomass to a protein-denaturing solution to produce a suspended biomass mixture, separating the solubilized biomass mixture into a supernatant phase and a decolorized biomass phase, hydrolyzing the decolorized biomass phase into a hydrolyzed product, fermenting the hydrolyzed product into an alcoholic solvent, mixing the alcoholic solvent and supernatant phase to produce a product suspension, and fractioning the product suspension into a bottom phase, middle phase, and top phase.

公开(公告)号：US20240076753A1

公开(公告)日：2024-03-07

申请号：US18501501

申请日：2023-11-03

Applicant: Fluid Quip Technologies, LLC

Inventor： Neal Jakel ,  Albert Pollmeier

IPC: C13K13/00 ,  A23L7/104 ,  A23L29/30 ,  C12M1/00 ,  C12P7/06 ,  C12P7/16 ,  C12P19/02 ,  C12P19/14 ,  C13K1/06

CPC classification number: C13K13/00 ,  A23L7/104 ,  A23L7/107 ,  A23L29/35 ,  C12M21/00 ,  C12P7/06 ,  C12P7/16 ,  C12P19/02 ,  C12P19/14 ,  C13K1/06

Abstract: An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biochemical production. In particular, after saccharification and prior to a sugar conversion process, a sugar/carbohydrate stream is removed from a saccharified stream. The sugar/carbohydrate stream includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose can be produced, with the such sugar stream being available for biochemical production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein and/or fiber. Sugar stream production occurs on the front end of the system and method.