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1.发明申请METHODS AND SYSTEMS FOR NUCLEIC ACID SEQUENCING VALIDATION, CALIBRATION AND NORMALIZATION 审中-公开用于核酸序列确认，校准和正常化的方法和系统
公开(公告)号：WO2011103467A2
公开(公告)日：2011-08-25
申请号：PCT/US2011/025486
申请日：2011-02-18
Applicant: LIFE TECHNOLOGIES CORPORATION , SHEN, Min-Yi , GREINER, Douglas
Inventor： SHEN, Min-Yi , GREINER, Douglas
CPC classification number: C12Q1/6874 , B01J2219/005 , C12Q2533/107 , C12Q2535/122 , G01N2201/1242 , G06F19/22 , C12Q2563/149 , C12Q2545/101
Abstract: A system for performing quality control for nucleic acid sample sequencing is disclosed. The system comprises a set of solid supports, each solid support having attached thereto a plurality of nucleic acid sequences, wherein the set comprises plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived, and the nucleic acids sequences are designed such that the nucleic acid sequences produce a predefined pattern of detectable signals during a sequencing run. A method of preparing a quality control for performing nucleic acid sample sequencing, a method of validating a nucleic acid sequencing instrument during a nucleic acid sequencing experiment, and a method of processing nucleic acid sequencing data during a nucleic acid sequencing experiment are also disclosed.
Abstract translation: 公开了一种用于进行核酸样品测序的质量控制的系统。 该系统包含一组固体支持物，每个固体支持物附着有多个核酸序列，其中该组包含多组固体支持物，并且每组含有与其相连的相同核酸序列的固体支持物。 各组的核酸序列彼此不同。 核酸序列是合成衍生的，并且设计核酸序列使得核酸序列在测序运行期间产生可检测信号的预定模式。 还公开了一种制备用于进行核酸样品测序的质量控制的方法，在核酸测序实验期间验证核酸测序仪的方法以及在核酸测序实验期间处理核酸测序数据的方法。
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2.发明申请DEVICE AND METHOD FOR MAKING DISCRETE VOLUMES OF A FIRST FLUID IN CONTACT WITH A SECOND FLUID, WHICH ARE IMMISCIBLE WITH EACH OTHER 审中-公开用于制造与第二流体接触的第一流体的离散体积的装置和方法，其与任何其它物质不相容
公开(公告)号：WO2007024800A3
公开(公告)日：2007-12-13
申请号：PCT/US2006032640
申请日：2006-08-22
Applicant: APPLERA CORP , COX DAVID M , WOO SAM L , REEL RICHARD T , LEHTO DENNIS A , LEE LINDA G , WIYATNO WILLY , OLDHAM MARK F , NURSE JAMES C , GREINER DOUGLAS P , WOJTOWICZ JANUSZ B
Inventor： COX DAVID M , WOO SAM L , REEL RICHARD T , LEHTO DENNIS A , LEE LINDA G , WIYATNO WILLY , OLDHAM MARK F , NURSE JAMES C , GREINER DOUGLAS P , WOJTOWICZ JANUSZ B
CPC classification number: C12Q1/6869 , B01J2219/00353 , B01J2219/0036 , B01J2219/00364 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00657 , B01J2219/00659 , B01L3/0293 , B01L3/502715 , B01L3/502784 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2300/0864 , B01L2300/0867 , B01L2400/0421 , B01L2400/0487 , C12Q1/6806 , C12Q1/6874 , F15C5/00 , F16K99/0001 , F16K99/0011 , F16K99/0013 , F16K2099/0084 , G01N1/14 , G01N27/44743 , G01N27/44769 , G01N35/08 , Y10T137/0318 , Y10T137/4259 , Y10T137/85978 , Y10T137/85986 , Y10T137/86863 , Y10T436/2575 , C12Q2535/101
Abstract: Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fiuid-discrete volumes separated by an oil.
Abstract translation: 在本申请中描述的各种实施例涉及用于在管道内产生与第二流体不混溶的一种或多种流体的离散体积的装置，系统和方法。 离散体积可用于涉及小体积的生物化学或分子生物学过程，例如微升体积，纳升级体积或更小体积。 该系统可以包括一个装置，该装置包括至少一个管道，其可操作地连接到一个或多个泵，用于通过与离散体积的流体不混溶的流体彼此分开的离散体积，例如含水混溶液 - 油分离体积。
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3.发明申请METHODS AND SYSTEMS FOR NUCLEIC ACID SEQUENCING VALIDATION, CALIBRATION AND NORMALIZATION 审中-公开用于核酸序列确认，校准和正常化的方法和系统
公开(公告)号：WO2011103467A3
公开(公告)日：2012-01-05
申请号：PCT/US2011025486
申请日：2011-02-18
Applicant: LIFE TECHNOLOGIES CORP , SHEN MIN-YI , GREINER DOUGLAS
Inventor： SHEN MIN-YI , GREINER DOUGLAS
CPC classification number: C12Q1/6874 , B01J2219/005 , C12Q2533/107 , C12Q2535/122 , G01N2201/1242 , G06F19/22 , C12Q2563/149 , C12Q2545/101
Abstract: A system for performing quality control for nucleic acid sample sequencing is disclosed. The system comprises a set of solid supports, each solid support having attached thereto a plurality of nucleic acid sequences, wherein the set comprises plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived, and the nucleic acids sequences are designed such that the nucleic acid sequences produce a predefined pattern of detectable signals during a sequencing run. A method of preparing a quality control for performing nucleic acid sample sequencing, a method of validating a nucleic acid sequencing instrument during a nucleic acid sequencing experiment, and a method of processing nucleic acid sequencing data during a nucleic acid sequencing experiment are also disclosed.
Abstract translation: 公开了一种用于进行核酸样品测序的质量控制的系统。 该系统包含一组固体支持物，每个固体支持物附着有多个核酸序列，其中该组包含多组固体支持物，并且每组含有与其相连的相同核酸序列的固体支持物。 各组的核酸序列彼此不同。 核酸序列是合成衍生的，并且设计核酸序列使得核酸序列在测序运行期间产生可检测信号的预定模式。 还公开了制备用于进行核酸样品测序的质量控制的方法，在核酸测序实验期间验证核酸测序仪的方法，以及在核酸测序实验期间处理核酸测序数据的方法。
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公开(公告)号：WO2010117456A9
公开(公告)日：2011-03-31
申请号：PCT/US2010001052
申请日：2010-04-08
Applicant: APPLIED BIOSYSTEMS LLC , LAU ALDRICH N K , KARGER ACHIM , KINNEY PATRICK , NURSE JAMES , KEVEL AFTAB , GREINER DOUGLAS , GJERSTAD CARMAN
Inventor： LAU ALDRICH N K , KARGER ACHIM , KINNEY PATRICK , NURSE JAMES , KEVEL AFTAB , GREINER DOUGLAS , GJERSTAD CARMAN
CPC classification number: B01F3/0807 , B01F7/161 , B01F7/22 , B01F2215/0422 , B01F2215/0431 , B01F2215/0481
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5.发明申请METHODS OF MODIFYING SUPPORT SURFACES FOR THE IMMOBILIZATION OF PARTICLES AND THE USE OF THE IMMOBILIZED PARTICLES FOR ANALYZING NUCLEIC ACIDS 审中-公开修饰支持表面的方法，用于颗粒的固定化和使用固定化颗粒分析核酸
公开(公告)号：WO2009026546A2
公开(公告)日：2009-02-26
申请号：PCT/US2008/074075
申请日：2008-08-22
Applicant: APPLIED BIOSYSTEMS INC. , GREINER, Douglas , STUART, Jeremy , TAING, Meng , MENCHEN, Steven, M.
Inventor： GREINER, Douglas , STUART, Jeremy , TAING, Meng , MENCHEN, Steven, M.
IPC: C40B40/04 , C40B20/04 , C40B40/06 , C40B50/18 , C40B40/08 , C07C335/20 , C07H21/00 , C12Q1/68 , C40B80/00
CPC classification number: C40B20/04 , C07B2200/11 , C07C335/20 , C07H21/00 , C12Q1/6869 , C40B40/04 , C40B40/06 , C40B40/08 , C40B50/18 , C40B80/00 , C12Q2565/518 , C12Q2521/501 , C12Q2523/107 , C12Q2533/101
Abstract: Methods of modifying a nucleophilic surface of a support are described. The methods involve reacting a multifunctional electrophilic reagent with nucleophilic groups on the surface of the support. The resulting electrophilic surface can be used for the covalent attachment of particles (e.g., beads) having nucleophilic functional groups. For example, nucleic acid templates with nucleophilic (e.g., amine) groups can be attached to a surface of the particles. The nucleophilic groups on the nucleic acid templates can then be used to attach the particles to the modified surface of the support. The resulting support-bound particles can be used to analyze (e.g., sequence) the nucleic acid templates on the particles.
Abstract translation: 描述了改变载体的亲核表面的方法。 所述方法包括使多功能亲电试剂与载体表面上的亲核基团反应。 所得亲电表面可用于共价连接具有亲核官能团的颗粒（例如珠）。 例如，具有亲核（例如胺）基团的核酸模板可以附着于颗粒的表面。 然后核酸模板上的亲核基团可用于将颗粒附着到载体的改性表面上。 得到的载体结合颗粒可用于分析（例如，序列）颗粒上的核酸模板。
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Patent Agency Ranking
公开(公告)号：WO2010117456A2
公开(公告)日：2010-10-14
申请号：PCT/US2010/001052
申请日：2010-04-08
Applicant: APPLIED BIOSYSTEMS, LLC , LAU, Aldrich, N., K. , KARGER, Achim , KINNEY, Patrick , NURSE, James , KEVEL, Aftab , GREINER, Douglas , GJERSTAD, Carman
Inventor： LAU, Aldrich, N., K. , KARGER, Achim , KINNEY, Patrick , NURSE, James , KEVEL, Aftab , GREINER, Douglas , GJERSTAD, Carman
CPC classification number: B01F3/0807 , B01F7/161 , B01F7/22 , B01F2215/0422 , B01F2215/0431 , B01F2215/0481
Abstract: An enrichment module and method are provided for enriching a population of templated beads and separating them from non-templated beads. The method can include hybridizing a templated bead with an enrichment bead to form a complex, trapping the complex in a filtration medium, washing non-templated beads through the filtration medium while retaining the complex, and then eluting the templated bead from the complex. The module can include a column for enrichment and filtration material exhibiting desired size- exclusion properties.
Abstract translation: 提供了一种富集模块和方法，用于富集模板珠群并将其与非模板珠分离。 该方法可以包括将模板珠与富集珠杂交以形成复合物，将复合物捕获在过滤介质中，通过过滤介质洗涤非模板珠，同时保留复合物，然后从络合物洗脱模板珠。 该模块可以包括用于富集和过滤材料的柱，其具有期望的尺寸排除性质。
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7.发明申请METHODS OF MODIFYING SUPPORT SURFACES FOR THE IMMOBILIZATION OF PARTICLES AND THE USE OF THE IMMOBILIZED PARTICLES FOR ANALYZING NUCLEIC ACIDS 审中-公开修改用于固定颗粒的支撑表面以及使用固定的颗粒分析核酸的方法
公开(公告)号：WO2009026546A3
公开(公告)日：2009-05-14
申请号：PCT/US2008074075
申请日：2008-08-22
Applicant: APPLIED BIOSYSTEMS , GREINER DOUGLAS , STUART JEREMY , TAING MENG , MENCHEN STEVEN M
Inventor： GREINER DOUGLAS , STUART JEREMY , TAING MENG , MENCHEN STEVEN M
IPC: C40B40/04 , C07C335/20 , C07H21/00 , C12Q1/68 , C40B20/04 , C40B40/06 , C40B40/08 , C40B50/18 , C40B80/00
CPC classification number: C40B20/04 , C07B2200/11 , C07C335/20 , C07H21/00 , C12Q1/6869 , C40B40/04 , C40B40/06 , C40B40/08 , C40B50/18 , C40B80/00 , C12Q2565/518 , C12Q2521/501 , C12Q2523/107 , C12Q2533/101
Abstract: Methods of modifying a nucleophilic surface of a support are described. The methods involve reacting a multifunctional electrophilic reagent with nucleophilic groups on the surface of the support. The resulting electrophilic surface can be used for the covalent attachment of particles (e.g., beads) having nucleophilic functional groups. For example, nucleic acid templates with nucleophilic (e.g., amine) groups can be attached to a surface of the particles. The nucleophilic groups on the nucleic acid templates can then be used to attach the particles to the modified surface of the support. The resulting support-bound particles can be used to analyze (e.g., sequence) the nucleic acid templates on the particles.
Abstract translation: 描述了改变载体的亲核表面的方法。 该方法包括使多官能亲电子试剂与载体表面上的亲核基团反应。 所得到的亲电表面可以用于共价连接具有亲核官能团的颗粒（例如珠粒）。 例如，具有亲核（例如胺）基团的核酸模板可以连接到颗粒的表面。 然后可以使用核酸模板上的亲核基团将颗粒附着到载体的改性表面。 得到的载体结合颗粒可用于分析（例如，测序）颗粒上的核酸模板。
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8.发明申请
公开(公告)号：WO2010028366A3
公开(公告)日：2010-03-11
申请号：PCT/US2009/056225
申请日：2009-09-08
Applicant: LIFE TECHNOLOGIES CORPORATION , GREINER, Douglas, P. , GJERSTAD, Carmen , ZIEGLE, Janet, S. , JONES, Lee, W. , SHEN, Min-Yi , CHIN, Jason , BREU, Heinz
Inventor： GREINER, Douglas, P. , GJERSTAD, Carmen , ZIEGLE, Janet, S. , JONES, Lee, W. , SHEN, Min-Yi , CHIN, Jason , BREU, Heinz
Abstract: A system for performing quality control for nucleic acid sample sequencing is disclosed. The system has a set of solid supports, each support having attached thereto a plurality of nucleic acid sequences. The set has plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived. A method of preparing a quality control for performing nucleic acid sample sequencing and a method of validating a nucleic acid sequencing instrument are also disclosed.
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公开(公告)号：WO2005114132A3
公开(公告)日：2007-03-22
申请号：PCT/US2005017813
申请日：2005-05-19
Applicant: QIAGEN SCIENCES INC , BELISLE CHRISTOPHER M , WALKER JOHN A II , NGOLA SARAH M , GREINER DOUGLAS P , LEVY MARK J , ZHAO XIAOXIA , CHEN IRENE Y , STOLOWITZ MARK L , PAQUIN DONALD P
Inventor： BELISLE CHRISTOPHER M , WALKER JOHN A II , NGOLA SARAH M , GREINER DOUGLAS P , LEVY MARK J , ZHAO XIAOXIA , CHEN IRENE Y , STOLOWITZ MARK L , PAQUIN DONALD P
CPC classification number: B01L3/5085 , B01L3/502707 , B01L3/502792 , B01L3/5088 , B01L2200/12 , B01L2300/087 , B01L2300/089 , B82Y15/00 , B82Y30/00 , C23C26/00 , C23C30/00 , Y10T436/24 , Y10T436/25
Abstract: The present invention relates to sample presentation devices useful in performing analytical measurements. These devices have been configured to enable various aspects of liquid handling such as: retention, storage, transport, concentration, positioning, and transfer. Additionally, these devices can enhance the detection and characterization of analytes. The sample presentation devices of the present invention are comprised of one or more substrates having a plurality of zones of differing wettability. Methods of analyzing samples using the sample presentation device of the invention, as well as methods of making the sample presentation devices are disclosed.
Abstract translation: 本发明涉及可用于执行分析测量的样本呈现装置。 这些装置已被配置成能够实现液体处理的各个方面，例如：保留，储存，运输，集中，定位和转移。 此外，这些设备可以增强分析物的检测和表征。 本发明的样品展示装置由具有不同润湿性的多个区域的一个或多个基材组成。 公开了使用本发明的样品呈现装置分析样品的方法，以及制备样品显示装置的方法。
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10.发明申请METHODS AND SYSTEMS FOR NUCLEIC ACID SEQUENCING VALIDATION, CALIBRATION AND NORMALIZATION 审中-公开用于核酸测序，校准和正常化的方法和系统
公开(公告)号：WO2010028366A2
公开(公告)日：2010-03-11
申请号：PCT/US2009056225
申请日：2009-09-08
Applicant: LIFE TECHNOLOGIES CORP , GREINER DOUGLAS P , GJERSTAD CARMEN , ZIEGLE JANET S , JONES LEE W , SHEN MIN-YI , CHIN JASON , BREU HEINZ
Inventor： GREINER DOUGLAS P , GJERSTAD CARMEN , ZIEGLE JANET S , JONES LEE W , SHEN MIN-YI , CHIN JASON , BREU HEINZ
CPC classification number: C12Q1/6874 , C12Q2545/101 , C12Q2563/149
Abstract: A system for performing quality control for nucleic acid sample sequencing is disclosed. The system has a set of solid supports, each support having attached thereto a plurality of nucleic acid sequences. The set has plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived. A method of preparing a quality control for performing nucleic acid sample sequencing and a method of validating a nucleic acid sequencing instrument are also disclosed.
Abstract translation: 公开了一种用于执行核酸样品测序的质量控制的系统。 该系统具有一组固体支持物，每个支持物附着有多个核酸序列。 该组具有多组固体支持物，并且每组包含具有与其相连的相同核酸序列的固体支持物。 各组的核酸序列彼此不同。 核酸序列是合成来源的。 还公开了制备用于执行核酸样品测序的质量控制的方法和验证核酸测序仪器的方法。
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