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    LABEL-FREE HIGH THROUGHPUT BIOMOLECULAR SCREENING SYSTEM AND METHOD
    2.
    发明申请
    LABEL-FREE HIGH THROUGHPUT BIOMOLECULAR SCREENING SYSTEM AND METHOD 失效
    无标签的高通量生物分子筛选系统和方法

    公开(公告)号：US20090247427A1

    公开(公告)日：2009-10-01

    申请号：US12480886

    申请日：2009-06-09

    申请人： Stephen J. Caracci ,  Volker H.O. Eckelt ,  Anthony G. Frutos ,  Mark F. Krol ,  Thomas C. Moore ,  David A. Pastel ,  Gordon M. Shedd

    发明人： Stephen J. Caracci ,  Volker H.O. Eckelt ,  Anthony G. Frutos ,  Mark F. Krol ,  Thomas C. Moore ,  David A. Pastel ,  Gordon M. Shedd

    IPC分类号： C40B60/12

    CPC分类号： G01N35/028 ,  G01N2035/00158 ,  G01N2035/00356 ,  G01N2035/0097

    摘要： A screening system and method are described herein which provide a unique and practical solution for enabling label-free high throughput screening (HTS) to aid in the discovery of new drugs. In one embodiment, the screening system enables direct binding assays to be performed in which a biomolecular interaction of a chemical compound (drug candidate) with a biomolecule (therapeutic target) can be detected using assay volumes and concentrations that are compatible with the current practices of HTS in the pharmaceutical industry. The screening system also enables the detection of bio-chemical interactions that occur in the wells of a microplate which incorporates biosensors and surface chemistry to immobilize the therapeutic target at the surface of the biosensors. The screening system also includes fluid handling and plate handling devices to help perform automated HTS assays.

    摘要翻译： 本文描述了一种筛选系统和方法，其提供用于实现无标记高通量筛选（HTS）以帮助发现新药物的独特且实用的解决方案。 在一个实施方案中，筛选系统使得能够进行直接结合测定，其中化学化合物（药物候选物）与生物分子（治疗靶标）的生物分子相互作用可以使用与当前实践相兼容的测定体积和浓度来检测 HTS在制药行业。 筛选系统还能够检测在微板的孔中发生的生物化学相互作用，其结合生物传感器和表面化学以将治疗靶固定在生物传感器的表面。 筛选系统还包括流体处理和板处理装置，以帮助执行自动HTS测定。

    SUBSTRATES WITH STABLE SURFACE CHEMISTRY FOR BIOLOGICAL MEMBRANE ARRAYS AND METHOD FOR FABRICATING THEREOF
    3.
    发明申请
    SUBSTRATES WITH STABLE SURFACE CHEMISTRY FOR BIOLOGICAL MEMBRANE ARRAYS AND METHOD FOR FABRICATING THEREOF 审中-公开
    用于生物膜阵列的稳定表面化学物质的基底及其制备方法

    公开(公告)号：US20090215650A1

    公开(公告)日：2009-08-27

    申请号：US12437893

    申请日：2009-05-08

    申请人： Ye Fang ,  Anthony G. Frutos ,  Joydeep Lahiri

    发明人： Ye Fang ,  Anthony G. Frutos ,  Joydeep Lahiri

    IPC分类号： C40B40/10

    CPC分类号： G01N33/54393 ,  B01J19/0046 ,  B01J2219/00385 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00635 ,  B01J2219/00659 ,  B01J2219/0074 ,  C40B60/14 ,  G01N33/54366

    摘要： The present invention provides a method for preparing a physically stable array of biological membranes, including membrane proteins, on a surface, and the resultant article of manufacture. The method comprises providing a substrate; creating either a polar surface or reactive surface by coating the substrate with a material that either: (1) enhances the stability of lipid spots during withdrawing through a water/air interface and washing and drying protocols; or (2) gives rise to minimal non-specific binding of a labeled target to a background surface, and high specific binding to a probe receptor in said membrane array, or (3) both; and depositing an array of biological-membrane microspots on the substrate. The method may further comprise applying a reagent that includes a soluable protein to stabilize the biological membranes on the surface. Also provided is an article having biological-membrane microspots that are associated in a stable fashion with a substrate surface embodying these properties.

    摘要翻译： 本发明提供了一种在表面上制备物理上稳定的生物膜阵列，包括膜蛋白的方法，以及所得制品。 该方法包括提供基底; 通过用以下材料涂覆基材来产生极性表面或反应性表面：（1）在通过水/空气界面排出并洗涤和干燥方案期间提高脂质斑点的稳定性; 或（2）导致标记的靶与背景表面的最小非特异性结合，以及所述膜阵列中与探针受体的高特异性结合，或（3）两者; 以及在衬底上沉积生物膜微点阵列。 该方法还可以包括施加包含可溶蛋白质的试剂以稳定表面上的生物膜。 还提供了具有生物膜微点的物品，其以稳定的方式与体现这些性质的基底表面相关联。

    Spatially scanned optical reader system and method for using same
    8.
    发明授权
    Spatially scanned optical reader system and method for using same 失效
    空间扫描光学阅读器系统及其使用方法

    公开(公告)号：US07604984B2

    公开(公告)日：2009-10-20

    申请号：US11027547

    申请日：2004-12-29

    申请人： Anthony G. Frutos ,  Jacques Gollier ,  Jinlin Peng ,  Garrett A. Piech ,  Michael B. Webb

    发明人： Anthony G. Frutos ,  Jacques Gollier ,  Jinlin Peng ,  Garrett A. Piech ,  Michael B. Webb

    IPC分类号： C12M1/34 ,  G01N21/00 ,  G01N21/01 ,  G01J3/00 ,  G01J3/28 ,  G02B6/00 ,  G02B6/34 ,  G02B26/08 ,  G02F1/07

    CPC分类号： G01N21/253 ,  G01N21/554 ,  G01N21/7743

    摘要： An optical reader system is described herein that uses a scanned optical beam to interrogate a biosensor to determine if a biomolecular binding event occurred on a surface of the biosensor. In one embodiment, the optical reader system includes a light source, a detector and a processor (e.g., computer, DSP). The light source outputs an optical beam which is scanned across a moving biosensor and while this is happening the detector collects the optical beam which is reflected from the biosensor. The computer processes the collected optical beam and records the resulting raw spectral or angle data which is a function of a position (and possibly time) on the biosensor. The processor can then analyze the raw data to create a spatial map of resonant wavelength (peak position) or resonant angle which indicates whether or not a biomolecular binding event occurred on the biosensor. Several other uses of the raw data are also described herein.

    摘要翻译： 本文描述了一种光学阅读器系统，其使用扫描的光束来询问生物传感器以确定生物分子结合事件是否在生物传感器的表面上发生。 在一个实施例中，光学读取器系统包括光源，检测器和处理器（例如，计算机，DSP）。 光源输出沿着移动的生物传感器扫描的光束，并且当发生这种情况时，检测器收集从生物传感器反射的光束。 计算机处理所收集的光束并记录所生成的原始光谱或角度数据，该数据是生物传感器上的位置（可能是时间）的函数。 然后，处理器可以分析原始数据以创建谐振波长（峰值位置）或共振角的空间图，其指示生物传感器上是否发生生物分子结合事件。 这里还描述了原始数据的几种其他用途。

    LABEL-FREE HIGH THROUGHPUT BIOMOLECULAR SCREENING SYSTEM AND METHOD
    10.
    发明申请
    LABEL-FREE HIGH THROUGHPUT BIOMOLECULAR SCREENING SYSTEM AND METHOD 审中-公开
    无标签的高通量生物分子筛选系统和方法

    公开(公告)号：US20120010105A1

    公开(公告)日：2012-01-12

    申请号：US13237316

    申请日：2011-09-20

    申请人： Stephen J. Caracci ,  Volker H.O. Eckelt ,  Anthony G. Frutos ,  Mark F. Krol ,  Thomas C. Moore ,  David A. Pastel ,  Gordon M. Shedd

    发明人： Stephen J. Caracci ,  Volker H.O. Eckelt ,  Anthony G. Frutos ,  Mark F. Krol ,  Thomas C. Moore ,  David A. Pastel ,  Gordon M. Shedd

    IPC分类号： C40B60/12 ,  C40B30/10 ,  C40B30/00

    CPC分类号： G01N35/028 ,  G01N2035/00158 ,  G01N2035/00356 ,  G01N2035/0097

    摘要： A screening system and method are described herein which provide a unique and practical solution for enabling label-free high throughput screening (HTS) to aid in the discovery of new drugs. In one embodiment, the screening system enables direct binding assays to be performed in which a biomolecular interaction of a chemical compound (drug candidate) with a biomolecule (therapeutic target) can be detected using assay volumes and concentrations that are compatible with the current practices of HTS in the pharmaceutical industry. The screening system also enables the detection of bio-chemical interactions that occur in the wells of a microplate which incorporates biosensors and surface chemistry to immobilize the therapeutic target at the surface of the biosensors. The screening system also includes fluid handling and plate handling devices to help perform automated HTS assays.

    摘要翻译： 本文描述了一种筛选系统和方法，其提供用于实现无标记高通量筛选（HTS）以帮助发现新药物的独特且实用的解决方案。 在一个实施方案中，筛选系统使得能够进行直接结合测定，其中化学化合物（药物候选物）与生物分子（治疗靶标）的生物分子相互作用可以使用与当前实践相兼容的测定体积和浓度来检测 HTS在制药行业。 筛选系统还能够检测在微板的孔中发生的生物化学相互作用，其结合生物传感器和表面化学以将治疗靶固定在生物传感器的表面。 筛选系统还包括流体处理和板处理装置，以帮助执行自动HTS测定。