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    Method for quantifying initial concentration of nucleic acid from real-time nucleic acid amplification data
    2.
    发明授权
    Method for quantifying initial concentration of nucleic acid from real-time nucleic acid amplification data 有权
    从实时核酸扩增数据定量核酸初始浓度的方法

    公开(公告)号：US08386191B2

    公开(公告)日：2013-02-26

    申请号：US13161788

    申请日：2011-06-16

    申请人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

    发明人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

    IPC分类号： G01N33/48

    CPC分类号： C12Q1/6851

    摘要： Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

    摘要翻译： 提供了从实时核酸扩增数据定量核酸的初始浓度的方法。 使用酶扩增从生物或病毒提取的核酸（DNA或RNA）。 然后，通过计算由核酸的背景荧光强度减去的核酸的荧光强度具有其最大值的一半的特征扩增循环数或特异性扩增时间来发现核酸的初始浓度，或 扩增效率具有由核酸的背景荧光强度减去的核酸的最大值或最小值或以前的扩增荧光强度的特征扩增循环数或特征扩增时间。 因此，可以在不分化或整合的情况下计算核酸的初始浓度。

    Method and apparatus for concentrating and amplifying nucleic acid in single micro chamber
    4.
    发明授权
    Method and apparatus for concentrating and amplifying nucleic acid in single micro chamber 有权
    在单微室中浓缩和扩增核酸的方法和装置

    公开(公告)号：US07807360B2

    公开(公告)日：2010-10-05

    申请号：US11620961

    申请日：2007-01-08

    申请人： Young-rok Kim ,  Jun-hong Min ,  In-ho Lee ,  Young-sun Lee ,  Chang-eun Yoo ,  Ki-woong Han

    发明人： Young-rok Kim ,  Jun-hong Min ,  In-ho Lee ,  Young-sun Lee ,  Chang-eun Yoo ,  Ki-woong Han

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6806 ,  B01L3/5027 ,  B01L3/5088 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2537/149 ,  C12Q2527/125

    摘要： A method of sequentially performing concentration and amplification of nucleic acid in a single micro chamber includes: introducing a nucleic acid-containing sample and a solution including a kosmotropic salt to a micro chamber having a hydrophilic interior surface to concentrate the nucleic acid by binding the nucleic acid on the interior surface of the micro chamber; and performing a polymerase chain reaction (PCR) by adding a PCR mixture to the chamber. Since the nucleic acid is reversibly bound to the interior surface of the micro chamber, PCR yield is higher compared with a surface of aluminum oxide in which irreversible binding occurs. In addition, all processes are sequentially performed in a single micro chamber so that the number of samples, consumables, time, and labor for treatment and analysis can be reduced, detection sensitivity can be improved, and risk of sample cross contamination significantly reduced without sample loss by eliminating transporting of the sample. A complete automated system for concentration and amplification of nucleic acid is thus readily provided.

    摘要翻译： 在单个微室中依次进行核酸的浓缩和扩增的方法包括：将含核酸的样品和包含可染色盐的溶液引入具有亲水性内表面的微室中，以通过结合核酸来浓缩核酸 酸在微室的内表面上; 并通过向该室加入PCR混合物进行聚合酶链式反应（PCR）。 由于核酸可逆地结合到微室的内表面，与其中发生不可逆结合的氧化铝的表面相比，PCR产率更高。 此外，所有过程都在单个微室中顺序进行，以便可以减少样品数量，消耗品，时间和处理和分析的劳动力，可以提高检测灵敏度，并且样品交叉污染的风险在没有样品时显着降低 通过消除样品的运输而损失。 因此容易提供用于浓缩和扩增核酸的完整的自动化系统。

    Microfluidic device for electrochemically regulating pH of fluid therein and method of regulating pH of fluid using the microfluidic device
    5.
    发明授权
    Microfluidic device for electrochemically regulating pH of fluid therein and method of regulating pH of fluid using the microfluidic device 有权
    用于电化学调节流体pH值的微流体装置和使用微流体装置调节流体pH值的方法

    公开(公告)号：US07771573B2

    公开(公告)日：2010-08-10

    申请号：US11489289

    申请日：2006-07-19

    申请人： Hun-joo Lee ,  Joon-ho Kim ,  Chin-sung Park ,  Young-sun Lee ,  Jong-Suk Chung

    发明人： Hun-joo Lee ,  Joon-ho Kim ,  Chin-sung Park ,  Young-sun Lee ,  Jong-Suk Chung

    IPC分类号： C25B9/00 ,  C02F1/461

    CPC分类号： B01D67/0062 ,  B01D2311/2623

    摘要： Provided is a microfluidic device for electrochemically regulating the pH of a fluid. The microfluidic device includes: an ion-exchange material; an anode chamber having a surface defined by a surface of the ion-exchange material and an anode electrode disposed along an edge of the surface of the anode chamber; and a cathode chamber having a surface defined by a surface of the ion-exchange material and a cathode electrode disposed along an edge of the surface of the cathode chamber, wherein the anode chamber and the cathode chamber are separated by an insulation material.

    摘要翻译： 提供了用于电化学调节流体pH的微流体装置。 微流体装置包括：离子交换材料; 具有由所述离子交换材料的表面限定的表面的阳极室和沿着所述阳极室的表面的边缘设置的阳极电极; 以及阴极室，其具有由离子交换材料的表面限定的表面和沿着阴极室的表面的边缘设置的阴极电极，其中阳极室和阴极室由绝缘材料分隔开。

    Method for quantifying initial concentration of nucleic acid from real-time nucleic acid amplification data
    6.
    发明授权
    Method for quantifying initial concentration of nucleic acid from real-time nucleic acid amplification data 有权
    从实时核酸扩增数据定量核酸初始浓度的方法

    公开(公告)号：US07698072B2

    公开(公告)日：2010-04-13

    申请号：US11217694

    申请日：2005-09-01

    申请人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

    发明人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

    IPC分类号： G06F19/00

    CPC分类号： C12Q1/6851

    摘要： Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

    摘要翻译： 提供了从实时核酸扩增数据定量核酸的初始浓度的方法。 使用酶扩增从生物或病毒提取的核酸（DNA或RNA）。 然后，通过计算由核酸的背景荧光强度减去的核酸的荧光强度具有其最大值的一半的特征扩增循环数或特异性扩增时间来发现核酸的初始浓度，或 扩增效率具有由核酸的背景荧光强度减去的核酸的最大值或最小值或以前的扩增荧光强度的特征扩增循环数或特征扩增时间。 因此，可以在不分化或整合的情况下计算核酸的初始浓度。