Process for producing a peptide using a serine or thiol proteinase
    10.
    发明授权
    Process for producing a peptide using a serine or thiol proteinase 失效
    使用丝氨酸或硫醇蛋白酶生产肽的方法

    公开(公告)号:US4086136A

    公开(公告)日:1978-04-25

    申请号:US732543

    申请日:1976-10-14

    摘要: A peptide having the formulaX--A--B--Y--wherein A and B are the same or different and each represents an amino acid residue or a peptide residue, X represents an amino protective group, Y represents a carboxyl protective group selected from the group consisting of tertiary alkoxy, and benzyloxy, benzylamino and benzhydrylamino which can be substituted with an inert substituent, is prepared by a process which comprises reacting an amino acid or peptide having an N-terminal protective group, or a salt thereof of the formula:X--A--OHwith an amino acid or peptide having a C-terminal protective group or a salt thereof of the formula:H--B--Yin the presence of a thiol proteinase or serine proteinase enzyme in an aqueous solution having a pH sufficient to maintain the enzyme activity of said thiol proteinase or serine proteinase.

    摘要翻译: 一种具有式XABY的肽,其中A和B相同或不同,各自表示氨基酸残基或肽残基,X表示氨基保护基,Y表示选自叔烷氧基, 和可以被惰性取代基取代的苄氧基,苄氨基和二苯甲基氨基,是通过包括使具有下式的N-末端保护基的氨基酸或肽或其盐反应的方法制备的: